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  1. Article: Hepatopancreas Proteomic Analysis Reveals Key Proteins and Pathways in Regulatory of Ovary Maturation of

    Jiang, Sufei / Qiao, Hui / Fu, Hongtuo / Gu, Zemao

    Animals : an open access journal from MDPI

    2023  Volume 13, Issue 6

    Abstract: A TMT-based (Tandem Mass Tag) liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomics approach was employed to explore differentially expressed proteins (DEPs) and KEGG pathways in hepatopancreas of 5 ovary stages. In total, 17,999 peptides ... ...

    Abstract A TMT-based (Tandem Mass Tag) liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomics approach was employed to explore differentially expressed proteins (DEPs) and KEGG pathways in hepatopancreas of 5 ovary stages. In total, 17,999 peptides were detected, among which 3395 proteins were identified. Further analysis revealed 26, 24, 37, and 308 DEPs in HE-I versus HE-II, HE-II versus HE-Ⅲ, HE-Ⅲ versus HE-Ⅳ, and HE-Ⅳ versus HE-Ⅴ, respectively (HE-I, HE-II, HE-III, HE-IV, and HE-V means hepatopancreas sampled from ovary stage I to V.). Gene ontology (GO) analysis indicated that DEPs were significantly enriched in "catalytic activity", "metabolic process", and "cell" of 4 comparison groups in turn. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results showed that in hepatopancreas, as the ovaries developed to maturation, carbohydrate metabolism, lipid metabolism, amino acid metabolism, and lysosome played important roles in turn. The mRNA expression of 15 selected DEPs were consistent with proteome results by qPCR analysis. Further mRNA expression investigation results suggested 4 proteins (fatty acid-binding protein, NPC intracellular cholesterol transporter 1, Serine hydroxymethyltransferase, and Crustapin) were involved in ovary maturation. These results enhance the understanding of the regulatory role of hepatopancreas in
    Language English
    Publishing date 2023-03-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13060977
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Hepatopancreas Proteomic Analysis Reveals Key Proteins and Pathways in Regulatory of Ovary Maturation of Macrobrachium nipponense

    Jiang, Sufei / Qiao, Hui / Fu, Hongtuo / Gu, Zemao

    Animals. 2023 Mar. 08, v. 13, no. 6

    2023  

    Abstract: A TMT-based (Tandem Mass Tag) liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomics approach was employed to explore differentially expressed proteins (DEPs) and KEGG pathways in hepatopancreas of 5 ovary stages. In total, 17,999 peptides ... ...

    Abstract A TMT-based (Tandem Mass Tag) liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomics approach was employed to explore differentially expressed proteins (DEPs) and KEGG pathways in hepatopancreas of 5 ovary stages. In total, 17,999 peptides were detected, among which 3395 proteins were identified. Further analysis revealed 26, 24, 37, and 308 DEPs in HE-I versus HE-II, HE-II versus HE-Ⅲ, HE-Ⅲ versus HE-Ⅳ, and HE-Ⅳ versus HE-Ⅴ, respectively (HE-I, HE-II, HE-III, HE-IV, and HE-V means hepatopancreas sampled from ovary stage I to V.). Gene ontology (GO) analysis indicated that DEPs were significantly enriched in “catalytic activity”, “metabolic process”, and “cell” of 4 comparison groups in turn. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results showed that in hepatopancreas, as the ovaries developed to maturation, carbohydrate metabolism, lipid metabolism, amino acid metabolism, and lysosome played important roles in turn. The mRNA expression of 15 selected DEPs were consistent with proteome results by qPCR analysis. Further mRNA expression investigation results suggested 4 proteins (fatty acid-binding protein, NPC intracellular cholesterol transporter 1, Serine hydroxymethyltransferase, and Crustapin) were involved in ovary maturation. These results enhance the understanding of the regulatory role of hepatopancreas in M. nipponense ovary maturation and provide new insights for understanding the crustacean regulation mechanisms.
    Keywords Macrobrachium nipponense ; amino acid metabolism ; carbohydrate metabolism ; cholesterol ; fatty acid-binding proteins ; gene expression ; gene expression regulation ; gene ontology ; genome ; glycine hydroxymethyltransferase ; hepatopancreas ; lipid metabolism ; liquid chromatography ; lysosomes ; peptides ; proteome ; proteomics ; tandem mass spectrometry
    Language English
    Dates of publication 2023-0308
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13060977
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Identification of Candidate Male-Reproduction-Related Genes from the Testis and Androgenic Gland of

    Jin, Shubo / Xiong, Yiwei / Zhang, Wenyi / Qiao, Hui / Wu, Yan / Jiang, Sufei / Fu, Hongtuo

    International journal of molecular sciences

    2024  Volume 25, Issue 3

    Abstract: The previous publication identified that pyruvate dehydrogenase E1 ( ...

    Abstract The previous publication identified that pyruvate dehydrogenase E1 (
    MeSH term(s) Animals ; Male ; Palaemonidae/genetics ; Testis/metabolism ; Pyruvate Dehydrogenase (Lipoamide)/genetics ; Androgens/metabolism ; Gene Expression Profiling/methods ; Reproduction ; Transcriptome
    Chemical Substances Pyruvate Dehydrogenase (Lipoamide) (EC 1.2.4.1) ; Androgens
    Language English
    Publishing date 2024-02-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25031940
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Identification of genes regulated by 20-Hydroxyecdysone in Macrobrachium nipponense using comparative transcriptomic analysis.

    Yuan, Huwei / Cai, Pengfei / Zhang, Wenyi / Jin, Shubo / Jiang, Sufei / Xiong, Yiwei / Gong, Yongsheng / Qiao, Hui / Fu, Hongtuo

    BMC genomics

    2024  Volume 25, Issue 1, Page(s) 35

    Abstract: Background: Macrobrachium nipponense is a freshwater prawn of economic importance in China. Its reproductive molt is crucial for seedling rearing and directly impacts the industry's economic efficiency. 20-hydroxyecdysone (20E) controls various ... ...

    Abstract Background: Macrobrachium nipponense is a freshwater prawn of economic importance in China. Its reproductive molt is crucial for seedling rearing and directly impacts the industry's economic efficiency. 20-hydroxyecdysone (20E) controls various physiological behaviors in crustaceans, among which is the initiation of molt. Previous studies have shown that 20E plays a vital role in regulating molt and oviposition in M. nipponense. However, research on the molecular mechanisms underlying the reproductive molt and role of 20E in M. nipponense is still limited.
    Results: A total of 240.24 Gb of data was obtained from 18 tissue samples by transcriptome sequencing, with > 6 Gb of clean reads per sample. The efficiency of comparison with the reference transcriptome ranged from 87.05 to 92.48%. A total of 2532 differentially expressed genes (DEGs) were identified. Eighty-seven DEGs associated with molt or 20E were screened in the transcriptomes of the different tissues sampled in both the experimental and control groups. The reliability of the RNA sequencing data was confirmed using Quantitative Real-Time PCR. The expression levels of the eight strong candidate genes showed significant variation at the different stages of molt.
    Conclusion: This study established the first transcriptome library for the different tissues of M. nipponense in response to 20E and demonstrated the dominant role of 20E in the molting process of this species. The discovery of a large number of 20E-regulated strong candidate DEGs further confirms the extensive regulatory role of 20E and provides a foundation for the deeper understanding of its molecular regulatory mechanisms.
    MeSH term(s) Female ; Animals ; Transcriptome ; Ecdysterone/pharmacology ; Palaemonidae/genetics ; Reproducibility of Results ; Gene Expression Profiling
    Chemical Substances Ecdysterone (5289-74-7)
    Language English
    Publishing date 2024-01-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041499-7
    ISSN 1471-2164 ; 1471-2164
    ISSN (online) 1471-2164
    ISSN 1471-2164
    DOI 10.1186/s12864-023-09927-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Effects of Alkalinity Exposure on Antioxidant Status, Metabolic Function, and Immune Response in the Hepatopancreas of

    Jin, Shubo / Xu, Mingjia / Gao, Xuanbin / Jiang, Sufei / Xiong, Yiwei / Zhang, Wenyi / Qiao, Hui / Wu, Yan / Fu, Hongtuo

    Antioxidants (Basel, Switzerland)

    2024  Volume 13, Issue 1

    Abstract: The oriental river ... ...

    Abstract The oriental river prawn
    Language English
    Publishing date 2024-01-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox13010129
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Role of

    Cai, Pengfei / Zhang, Wenyi / Jiang, Sufei / Xiong, Yiwei / Qiao, Hui / Yuan, Huwei / Gao, Zijian / Zhou, Yongkang / Jin, Shubo / Fu, Hongtuo

    International journal of molecular sciences

    2024  Volume 25, Issue 3

    Abstract: This study investigates the role of lysosomal acid ... ...

    Abstract This study investigates the role of lysosomal acid lipase
    MeSH term(s) Animals ; Male ; Palaemonidae/metabolism ; Semen/metabolism ; Gonadal Steroid Hormones/metabolism ; Cholesterol/metabolism ; Triglycerides/metabolism ; Arthropod Proteins/genetics ; Arthropod Proteins/metabolism
    Chemical Substances Gonadal Steroid Hormones ; Cholesterol (97C5T2UQ7J) ; Triglycerides ; Arthropod Proteins
    Language English
    Publishing date 2024-01-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25031399
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Identifying Relationships between

    Gao, Xuanbin / Gao, Zijian / Zhang, Minglei / Qiao, Hui / Jiang, Sufei / Zhang, Wenyi / Xiong, Yiwei / Jin, Shubo / Fu, Hongtuo

    Animals : an open access journal from MDPI

    2024  Volume 14, Issue 5

    Abstract: Investigating hypoxia tolerance and growth trait single nucleotide polymorphisms (SNPs) ... ...

    Abstract Investigating hypoxia tolerance and growth trait single nucleotide polymorphisms (SNPs) in
    Language English
    Publishing date 2024-02-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani14050666
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Identification of Male Sex-Related Genes Regulated by

    Gao, Zijian / Zhang, Wenyi / Jiang, Sufei / Yuan, Huwei / Cai, Pengfei / Jin, Shubo / Fu, Hongtuo

    International journal of molecular sciences

    2023  Volume 24, Issue 17

    Abstract: The oriental river prawn ( ...

    Abstract The oriental river prawn (
    MeSH term(s) Male ; Animals ; Palaemonidae/genetics ; RNA Interference ; Sperm Motility ; Gene Expression Profiling ; Androgens ; Insulin
    Chemical Substances Androgens ; Insulin
    Language English
    Publishing date 2023-08-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241713176
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: RNAi Analysis of Potential Functions of Cyclin B3 in Reproduction of Male Oriental River Prawns (Macrobrachium nipponense)

    Jin, Shubo / Zhou, Zhenyu / Zhang, Wenyi / Xiong, Yiwei / Qiao, Hui / Gong, Yongsheng / Wu, Yan / Jiang, Sufei / Fu, Hongtuo

    Animals. 2023 May 21, v. 13, no. 10

    2023  

    Abstract: Cyclin B3 (CycB3) is involved in the metabolic pathway of the cell cycle, playing essential roles in the regulation of cell proliferation and mitosis. CycB3 is also predicted to be involved in the reproduction of male oriental river prawns (Macrobrachium ...

    Abstract Cyclin B3 (CycB3) is involved in the metabolic pathway of the cell cycle, playing essential roles in the regulation of cell proliferation and mitosis. CycB3 is also predicted to be involved in the reproduction of male oriental river prawns (Macrobrachium nipponense). In this study, the potential functions of CycB3 in M. nipponense were investigated using quantitative real-time PCR, RNA interference, and histological observations. The full-length DNA sequence of CycB3 in M. nipponense was 2147 base pairs (bp) long. An open reading frame of 1500 bp was found, encoding 499 amino acids. A highly conserved destruction box and two conserved cyclin motifs were found in the protein sequence of Mn-CycB3. Phylogenetic tree analysis revealed that this protein sequence was evolutionarily close to that of CycB3s of crustacean species. Quantitative real-time PCR analysis results suggested that CycB3 was involved in the process of spermiogenesis, oogenesis, and embryogenesis in M. nipponense. RNA interference analysis showed that CycB3 had a positive regulatory relationship with insulin-like androgenic gland hormone (IAG) in M. nipponense. In addition, sperm were rarely observed in the testis of double-stranded CycB3-injected prawns after 14 days of treatment, and sperm abundance was dramatically lower than that in the double-stranded GFP-injected prawns on the same day. This result indicated that CycB3 can regulate the testis reproduction in M. nipponense through inhibiting the IAG expressions. Overall, these results indicated that CycB3 plays essential roles in the regulation of male reproduction in M. nipponense, which may promote the studies of male reproduction in other crustacean species.
    Keywords Macrobrachium nipponense ; RNA interference ; amino acid sequences ; biochemical pathways ; cell proliferation ; cyclins ; embryogenesis ; histology ; males ; mitosis ; nucleotide sequences ; oogenesis ; phylogeny ; quantitative polymerase chain reaction ; rivers ; spermatozoa ; spermiogenesis ; testes
    Language English
    Dates of publication 2023-0521
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13101703
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Mn-XRN1 Has an Inhibitory Effect on Ovarian Reproduction in Macrobrachium nipponense

    Chen, Tianyong / Yuan, Huwei / Qiao, Hui / Jiang, Sufei / Zhang, Wenyi / Xiong, Yiwei / Fu, Hongtuo / Jin, Shubo

    Genes (Basel). 2023 July 16, v. 14, no. 7

    2023  

    Abstract: XRN1 is an exoribonuclease that degrades mRNA in the cytoplasm along the 5′–3′ direction. A previous study indicated that it may be involved in the reproduction of Macrobrachium nipponense. Quantitative real-time PCR was used to detect the spatiotemporal ...

    Abstract XRN1 is an exoribonuclease that degrades mRNA in the cytoplasm along the 5′–3′ direction. A previous study indicated that it may be involved in the reproduction of Macrobrachium nipponense. Quantitative real-time PCR was used to detect the spatiotemporal expression pattern of Mn-XRN1. At the tissue level, Mn-XRN1 was significantly expressed in the ovary. During development, Mn-XRN1 was significantly expressed at the CS stage of the embryo, on the 10th day post-larval and in the O2 stage of ovarian reproduction. The in situ hybridization results showed the location of Mn-XRN1 in the ovary. The expression of Mn-VASA was significantly increased after in vivo injection of Mn-XRN1 dsRNA. This suggests that Mn-XRN1 negatively regulates the expression of Mn-VASA. Furthermore, we counted the number of M. nipponense at various stages of ovarian reproduction on different days after RNAi. The results showed that ovarian development was significantly accelerated. In general, the results of the present study indicate that Mn-XRN1 has an inhibitory effect on the ovarian maturation of M. nipponense. The inhibitory effect might be through negative regulation of Mn-VASA.
    Keywords Macrobrachium nipponense ; cytoplasm ; exoribonucleases ; hybridization ; ovarian development ; postlarvae ; quantitative polymerase chain reaction
    Language English
    Dates of publication 2023-0716
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14071454
    Database NAL-Catalogue (AGRICOLA)

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