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  1. Article: [Regulatory effects of bio-intensity electric field on transformation of human skin fibroblasts].

    Wang, W P / Ji, R / Zhang, Z / Wu, Y T / Zhang, H S / Zhang, Q / Jiang, X P / Teng, Miao

    Zhonghua shao shang yu chuang mian xiu fu za zhi

    2022  Volume 38, Issue 4, Page(s) 354–362

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Actins/biosynthesis ; Cell Differentiation/physiology ; Cell Movement/physiology ; Electric Stimulation Therapy ; Electricity ; Fibroblasts/metabolism ; Fibroblasts/physiology ; Humans ; Myofibroblasts/metabolism ; Myofibroblasts/physiology ; Proliferating Cell Nuclear Antigen/biosynthesis ; Skin/cytology
    Chemical Substances ACTA2 protein, human ; Actins ; Proliferating Cell Nuclear Antigen
    Language Chinese
    Publishing date 2022-04-21
    Publishing country China
    Document type Journal Article
    ISSN 2097-1109
    ISSN 2097-1109
    DOI 10.3760/cma.j.cn501120-20210112-00017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: [Aesthetic reconstruction of the scar contracture deformity in chin and neck with expanded flaps based on the "MRIS" principle].

    Zhang, J P / Yuan, X / Jiang, X P / Liu, J / Chen, Z / Li, Y P / Wang, H X

    Zhonghua shao shang yu chuang mian xiu fu za zhi

    2022  Volume 38, Issue 4, Page(s) 306–312

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Chin/surgery ; Cicatrix/surgery ; Contracture/etiology ; Contracture/surgery ; Female ; Free Tissue Flaps ; Humans ; Male ; Perforator Flap ; Plastic Surgery Procedures ; Skin Transplantation ; Surgical Wound ; Treatment Outcome
    Language Chinese
    Publishing date 2022-04-21
    Publishing country China
    Document type Journal Article ; Observational Study
    ISSN 2097-1109
    ISSN 2097-1109
    DOI 10.3760/cma.j.cn501120-20211130-00401
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: [Effects of bio-strength electric field on the motility and CD9 expression of human epidermal cell line HaCaT and mouse epidermal cells].

    Ji, R / Zhang, Z / Wang, W P / Zhang, Q / Lyu, Y L / Jiang, X P / Teng, M

    Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns

    2021  Volume 37, Issue 1, Page(s) 34–41

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Animals ; Cell Line ; Cell Movement ; Epidermal Cells ; Epidermis ; Female ; Humans ; Mice ; Mice, Inbred BALB C
    Language Chinese
    Publishing date 2021-01-22
    Publishing country China
    Document type Journal Article
    ZDB-ID 2229222-6
    ISSN 1009-2587
    ISSN 1009-2587
    DOI 10.3760/cma.j.cn501120-20200115-00023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6330: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: [Regulatory effects of bio-intensity electric field on microtubule acetylation in human epidermal cell line HaCaT].

    Wu, Y T / Zhang, Z / Ji, R / Zhang, S H / Wang, W P / Wu, C / Zhang, J P / Jiang, X P / Zhang, Hengshu

    Zhonghua shao shang yu chuang mian xiu fu za zhi

    2022  Volume 38, Issue 11, Page(s) 1066–1072

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Humans ; Acetylation ; Tubulin/analysis ; Tubulin/metabolism ; Microtubules/chemistry ; Microtubules/metabolism ; Electricity ; Epidermal Cells/chemistry ; Epidermal Cells/metabolism
    Chemical Substances Tubulin
    Language Chinese
    Publishing date 2022-11-23
    Publishing country China
    Document type English Abstract ; Journal Article
    ISSN 2097-1109
    ISSN 2097-1109
    DOI 10.3760/cma.j.cn501120-20211105-00377
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Mitochondria organelle transplantation: introduction of normal epithelial mitochondria into human cancer cells inhibits proliferation and increases drug sensitivity.

    Elliott, R L / Jiang, X P / Head, J F

    Breast cancer research and treatment

    2012  Volume 136, Issue 2, Page(s) 347–354

    Abstract: Mitochondrial dysfunction of cancer cells includes increased aerobic glycolysis, elevated levels of ROS, decreased apoptosis, and resistance to chemotherapeutic agents. We hypothesized that the introduction of normal mitochondria into cancer cells might ... ...

    Abstract Mitochondrial dysfunction of cancer cells includes increased aerobic glycolysis, elevated levels of ROS, decreased apoptosis, and resistance to chemotherapeutic agents. We hypothesized that the introduction of normal mitochondria into cancer cells might restore mitochondrial function and inhibit cancer cell growth, and reverse chemoresistance. First, in the present study, we tested if mitochondria of immortalized, untransformed mammary epithelial MCF-12A cells could enter into human cancer cell lines. Second, if introducing normal mitochondria into cancer cells would inhibit proliferation. And third, would the addition of normal mitochondria increase the sensitivity of human breast cancer MCF-7 cells to chemotherapy. We found that JC-1-stained mitochondria of immortalized, untransformed mammary epithelial MCF-12A cells can enter into the cancer cell lines MCF-7, MDA-MB-231, and NCI/ADR-Res, but cannot enter immortalized, untransformed MCF-12A cells. The normal mitochondria from immortalized, untransformed MCF-12A cells suppressed the proliferation of MCF-7 and NCI/ADR-Res cells in a dose-dependent pattern, but did not affect the proliferation of immortalized, untransformed MCF-12A cells. The normal mitochondria from immortalized, untransformed MCF-12A cells increased the sensitivity of human breast cancer MCF-7 cells to doxorubicin, Abraxane, and carboplatin. In conclusion, the introduction of normal mammary mitochondria into human breast cancer cells inhibits cancer cell proliferation and increases the sensitivity of the MCF-7 human breast cancer cell line to doxorubicin, Abraxane, and carboplatin. These results support the role of mitochondrial dysfunction in cancer and suggest the possible use of targeted mitochondria for cancer therapeutics.
    MeSH term(s) Breast Neoplasms/metabolism ; Cell Line, Tumor ; Cell Proliferation ; Drug Resistance, Neoplasm ; Epithelial Cells/chemistry ; Epithelial Cells/metabolism ; Female ; Humans ; MCF-7 Cells ; Mammary Glands, Human ; Mitochondria/metabolism ; Mitochondria/transplantation
    Language English
    Publishing date 2012-11
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 604563-7
    ISSN 1573-7217 ; 0167-6806
    ISSN (online) 1573-7217
    ISSN 0167-6806
    DOI 10.1007/s10549-012-2283-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1655: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Audio / Video: Comparing antibiotic resistance in commensal and pathogenic bacteria isolated from wild-caught South Carolina shrimps vs. farm-raised imported shrimps

    Boinapally, K / Jiang, X.P

    Canadian journal of microbiology. 2007 July, v. 53, no. 7

    2007  

    Keywords shrimp ; seafoods ; imports ; shrimp culture ; bacteria ; animal pathogenic bacteria ; antibiotic resistance ; tetracycline ; cephalosporins ; Salmonella ; Vibrio vulnificus ; Escherichia coli ; food contamination ; bacterial contamination ; drug resistance ; ceftriaxone ; South Carolina
    Language English
    Dates of publication 2007-07
    Size p. 919-924.
    Document type Article ; Audio / Video
    Note Summary in French.
    ZDB-ID 280534-0
    ISSN 1480-3275 ; 0008-4166
    ISSN (online) 1480-3275
    ISSN 0008-4166
    DOI 10.1139/W07-019 |
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 71/748: Show issues

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  7. Article ; Online: Constitutive expression, purification and characterization of bovine prochymosin in Pichia pastoris GS115.

    Jiang, X P / Yin, M L / Chen, P / Yang, Q

    World journal of microbiology & biotechnology

    2012  Volume 28, Issue 5, Page(s) 2087–2093

    Abstract: Chymosin can specifically break down the Phe105-Met106 peptide bond of milk κ-casein to form insoluble para-κ-casein, resulting in milk coagulation, a process that is used in making cheese. In this study, in order to obtain an alternative milk coagulant ... ...

    Abstract Chymosin can specifically break down the Phe105-Met106 peptide bond of milk κ-casein to form insoluble para-κ-casein, resulting in milk coagulation, a process that is used in making cheese. In this study, in order to obtain an alternative milk coagulant which is safe and efficient, and simultaneously can produce cheese with a good taste, bovine prochymosin B was chosen and constitutively expressed to a high level in Pichia pastoris. The recombinant chymosin was expressed mainly as a secretory form, and it exhibited milk-clotting activity. It was purified by ammonium sulfate fractionation, anion exchange, followed by cation exchange chromatography. A final yield of 24.2% was obtained for the purified enzyme, which appeared as a single band in SDS-PAGE having a molecular mass of approximate 36 kDa. Proteolysis assay showed that it specifically hydrolyzed κ-casein. It was stable at 25-50°C and had optimal activity at 37°C and pH 4.0. The activity of the recombinant chymosin was activated by cations such as Mn(2+), Fe(3+), Mg(2+) and Na(+), but inhibited by K(+), Co(2+), Zn(2+), Ni(2+), and to a lesser extent by Cu(2+). These results suggested that recombinant bovine chymosin is an acid milk coagulant, and it could be considered as a safe and efficient enzyme suitable for use in cheese production.
    MeSH term(s) Animals ; Caseins/metabolism ; Cattle ; Chemical Precipitation ; Chromatography, Ion Exchange ; Chymosin/biosynthesis ; Chymosin/chemistry ; Chymosin/genetics ; Chymosin/isolation & purification ; Electrophoresis, Polyacrylamide Gel ; Enzyme Activators/metabolism ; Enzyme Precursors/biosynthesis ; Enzyme Precursors/chemistry ; Enzyme Precursors/genetics ; Enzyme Precursors/isolation & purification ; Enzyme Stability ; Gene Expression ; Hydrogen-Ion Concentration ; Hydrolysis ; Metals/metabolism ; Milk/metabolism ; Molecular Weight ; Pichia/genetics ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification ; Temperature
    Chemical Substances Caseins ; Enzyme Activators ; Enzyme Precursors ; Metals ; Recombinant Proteins ; prorennin (EC 3.4.23.-) ; Chymosin (EC 3.4.23.4)
    Language English
    Publishing date 2012-02-10
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1499109-3
    ISSN 1573-0972 ; 0959-3993
    ISSN (online) 1573-0972
    ISSN 0959-3993
    DOI 10.1007/s11274-012-1012-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: [Role of FibroScan in liver fibrosis evaluation in patients with chronic hepatitis B virus infection and related influencing factors].

    Xie, Q X / Xu, N / Jiang, X P / Zhang, Y F / Zhang, Z H / Li, J B / Hu, X Y / Li, X

    Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology

    2016  Volume 24, Issue 9, Page(s) 659–664

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Adult ; Alanine Transaminase/blood ; Biomarkers/blood ; Chronic Disease ; Elasticity ; Elasticity Imaging Techniques ; Female ; Hepatitis B e Antigens ; Hepatitis B, Chronic/complications ; Hepatitis B, Chronic/diagnostic imaging ; Hepatitis B, Chronic/physiopathology ; Humans ; Liver/diagnostic imaging ; Liver/pathology ; Liver Cirrhosis/complications ; Liver Cirrhosis/diagnostic imaging ; Liver Cirrhosis/physiopathology ; Male ; Middle Aged ; ROC Curve ; Serologic Tests
    Chemical Substances Biomarkers ; Hepatitis B e Antigens ; Alanine Transaminase (EC 2.6.1.2)
    Language Chinese
    Publishing date 2016-09-20
    Publishing country China
    Document type Journal Article
    ISSN 1007-3418
    ISSN 1007-3418
    DOI 10.3760/cma.j.issn.1007-3418.2016.09.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Hepatitis B Surface Antigen S Gene is an Effective Carrier Molecule for Developing GnRH DNA Immunocastration Vaccine in Mice.

    Han, Y G / Ye, W J / Liu, G Q / Jiang, X P / Ijaz, N / Zhao, J Y / Tesema, B

    Reproduction in domestic animals = Zuchthygiene

    2016  Volume 51, Issue 3, Page(s) 445–450

    Abstract: Relatively molecular mass of GnRH antigens is small and hence needs to couple to a large carrier molecule to enhance its immunogenicity. This study investigated whether hepatitis B surface antigen S (HBsAg-S) gene can be used as an effective carrier ... ...

    Abstract Relatively molecular mass of GnRH antigens is small and hence needs to couple to a large carrier molecule to enhance its immunogenicity. This study investigated whether hepatitis B surface antigen S (HBsAg-S) gene can be used as an effective carrier molecule for developing GnRH DNA immunocastration vaccine. Two copies of human GnRH gene were fused with HBsAg-S gene for constructing a recombinant plasmid pVAX-HBsAg-S-2GnRH that coded for 27 kDa target fusion protein. Ten male mice were divided into two equal groups, treatment and control. The vaccine (50 μg/mice) prepared in saline solution was injected into male mice at weeks 0, 1, 2, 4 and 7 of the experiment. Vaccine's efficacy was evaluated in terms of GnRH-specific IgG antibody response, plasma testosterone levels, testicular weight and extent of the testicular tissue damage. The specific anti-GnRH antibody titre in vaccinated animals was significantly higher than in controls in only 4th week of immunization (p < 0.05). In addition, vaccinated animals showed lower testicular weight than those of the controls (p < 0.05). Spermatogenesis in seminiferous tubules in vaccinated animals was suppressed. In conclusion, in this study, the engineered plasmid to be used as a GnRH DNA vaccine induced antibody response and suppressed spermatogenesis in mice. This suggests that HBsAg-S gene can be an effective carrier molecule for developing GnRH DNA immunocastration vaccine when relatively molecular mass of the aimed antigens is small.
    MeSH term(s) Animals ; Gonadotropin-Releasing Hormone/genetics ; Gonadotropin-Releasing Hormone/immunology ; Hepatitis B Surface Antigens/genetics ; Humans ; Immunization ; Immunoglobulin G/blood ; Male ; Mice ; Organ Size ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/immunology ; Spermatogenesis/immunology ; Sterilization, Reproductive/methods ; Testis/anatomy & histology ; Testosterone/blood ; Vaccines, DNA
    Chemical Substances Hepatitis B Surface Antigens ; Immunoglobulin G ; Recombinant Fusion Proteins ; Vaccines, DNA ; Gonadotropin-Releasing Hormone (33515-09-2) ; Testosterone (3XMK78S47O)
    Language English
    Publishing date 2016-06
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1015187-4
    ISSN 1439-0531 ; 0936-6768
    ISSN (online) 1439-0531
    ISSN 0936-6768
    DOI 10.1111/rda.12692
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 2117: Show issues

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  10. Article: Constitutive expression, purification and characterization of bovine prochymosin in Pichia pastoris GS115

    Jiang, X. P / Yin, M. L / Chen, P / Yang, Q

    World journal of microbiology and biotechnology. 2012 May, v. 28, no. 5

    2012  

    Abstract: Chymosin can specifically break down the Phe105–Met106 peptide bond of milk κ-casein to form insoluble para-κ-casein, resulting in milk coagulation, a process that is used in making cheese. In this study, in order to obtain an alternative milk coagulant ... ...

    Abstract Chymosin can specifically break down the Phe105–Met106 peptide bond of milk κ-casein to form insoluble para-κ-casein, resulting in milk coagulation, a process that is used in making cheese. In this study, in order to obtain an alternative milk coagulant which is safe and efficient, and simultaneously can produce cheese with a good taste, bovine prochymosin B was chosen and constitutively expressed to a high level in Pichia pastoris. The recombinant chymosin was expressed mainly as a secretory form, and it exhibited milk-clotting activity. It was purified by ammonium sulfate fractionation, anion exchange, followed by cation exchange chromatography. A final yield of 24.2% was obtained for the purified enzyme, which appeared as a single band in SDS–PAGE having a molecular mass of approximate 36 kDa. Proteolysis assay showed that it specifically hydrolyzed κ-casein. It was stable at 25–50°C and had optimal activity at 37°C and pH 4.0. The activity of the recombinant chymosin was activated by cations such as Mn2+, Fe3+, Mg2+ and Na+, but inhibited by K+, Co2+, Zn2+, Ni2+, and to a lesser extent by Cu2+. These results suggested that recombinant bovine chymosin is an acid milk coagulant, and it could be considered as a safe and efficient enzyme suitable for use in cheese production.
    Keywords Pichia pastoris ; ammonium sulfate ; anion exchange ; cation exchange ; cations ; cattle ; cheesemaking ; cheeses ; chromatography ; chymosin ; coagulation ; fractionation ; gene expression ; milk ; molecular weight ; pH ; proteolysis ; taste
    Language English
    Dates of publication 2012-05
    Size p. 2087-2093.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 1499109-3
    ISSN 1573-0972 ; 0959-3993
    ISSN (online) 1573-0972
    ISSN 0959-3993
    DOI 10.1007/s11274-012-1012-7
    Database NAL-Catalogue (AGRICOLA)

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