LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 4 of total 4

Search options

  1. Article ; Online: Detection of Variants of B.1.617 Lineage from Five Returning Chinese Nationals at a Guangxi-Vietnam Border Port - Guangxi Zhuang Autonomous Region, China, April 2021.

    Kang, Ning / Liu, Yinpin / Bi, Fuyin / Wang, Jing / Chen, Huafeng / Liang, Zhenli / Chen, Min / Wei, Guangwu / Li, Aiming / Li, Yonghong / Lu, Bao / Jiang, Zhenling / Tan, Yi / Zhong, Ge / Lin, Mei / Ju, Yu

    China CDC weekly

    2021  Volume 3, Issue 30, Page(s) 653–655

    Language English
    Publishing date 2021-09-14
    Publishing country China
    Document type Journal Article
    ISSN 2096-7071
    ISSN (online) 2096-7071
    DOI 10.46234/ccdcw2021.147
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article: [Epidemiological characteristics of typhoid fever and antibiotic susceptibility testing of Salmonella Typhi isolates in Guangxi, 1994-2013].

    Wang, Mingliu / Kan, Biao / Yang, Jin / Lin, Mei / Yan, Meiying / Zeng, Jun / Quan, Yi / Liao, Hezhuang / Zhou, Lingyun / Jiang, Zhenling / Huang, Dehui

    Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi

    2014  Volume 35, Issue 8, Page(s) 930–934

    Abstract: Objective: Through analyzing the typhoid epidemics and to determine and monitor regional resistance characteristics of the shift of drug resistant profile on Salmonella (S.) Typhi, to understand the related epidemiological characteristics of typhoid ... ...

    Abstract Objective: Through analyzing the typhoid epidemics and to determine and monitor regional resistance characteristics of the shift of drug resistant profile on Salmonella (S.) Typhi, to understand the related epidemiological characteristics of typhoid fever and to provide evidence for the development of strategies, in Guangxi.
    Methods: Data of typhoid fever from surveillance and reporting system between 1994 to 2013 was collected and statistically analyzed epidemiologically. The susceptibility of 475 S. Typhi isolates from patients on ten antibiotics was tested by broth micro-dilution method and minimum inhibition concentration was obtained and interpreted based on the CLSI standard.
    Results: From 1994 to 2013, a total of 57 928 cases of typhoid fever were reported in Guangxi province with an annual incidence of 6.29/100 000 and mortality as 0.03%. The higher incidence was observed in the population under 20 years of age. There was no significant difference on incidence between male and female, but farmers and students were among the hardest hit groups. More cases were seen from the northern part of the province. Cases appeared all year round with the peak from May to October. A total of 13 major outbreaks during 2001 to 2013 were reported and the main transmission route was water-borne. All the strains were sensitive to third generation cephalosporins cefotaxime and fluoroquinolones norfloxacin. The susceptibility rates to tetracycline, chloramphenicol, ampicillin and gentamicin was around 98% but relative lower susceptible rate to ciprofloxacin was seen as 89.89% . The lowest susceptibility was found for streptomycin and sulfamethoxazole agents, with the rates as 67.73% and 65.89% , respectively. One strain was found to have been resistant to ciprofloxacin and another 47 isolates with reduced susceptibility to ciprofloxacin. Twenty eight isolates were found to be resistant to multiple antibiotics and one displayed ampicillin, chloramphenicol, streptomycin, sulfamethoxazole tetracycline and nalidixic acid (ACSSxT-NAL) resistance profile. This was the first report in China. Multi-drug resistant strains were frequently isolated from small scale outbreaks of typhoid fever.
    Conclusion: The incidence of typhoid fever in Guangxi was still high and some strains showed multi-drug resistance and reduced susceptibility to ciprofloxacin, indicating that the surveillance and monitor programs on drug resistance of S. Typhi should be strengthened, to prevent large scale outbreaks of typhoid fever in this province.
    MeSH term(s) China/epidemiology ; Disease Outbreaks ; Drug Resistance, Multiple, Bacterial ; Female ; Humans ; Male ; Salmonella typhi/drug effects ; Salmonella typhi/isolation & purification ; Typhoid Fever/epidemiology
    Language Chinese
    Publishing date 2014-08
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 645026-x
    ISSN 0254-6450
    ISSN 0254-6450
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 3276: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article: [Optimization of T7-based RNA amplification system for cDNA microarray].

    Li, Tao / Li, Yao / Han, Zhi-Yong / Chen, Qin / Qiu, Min-Yan / Ni, Sheng / Jiang, Zhen-Ling / Xie, Yi / Mao, Yu-Min

    Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

    2003  Volume 35, Issue 5, Page(s) 441–448

    Abstract: cDNA microarrays are powerful parallel tools for gene expression profiling analysis, which help us to understand the molecular mechanism of diseases and to identify potential targets for therapeutic intervention. However, their broader application are ... ...

    Abstract cDNA microarrays are powerful parallel tools for gene expression profiling analysis, which help us to understand the molecular mechanism of diseases and to identify potential targets for therapeutic intervention. However, their broader application are hampered by the large amount of RNA required: up to 200 microg of total RNA or 5 microg of mRNA for one chip, making analysis of small samples difficult. In this work, combined with a template switching effect, the T7 RNA linear amplification procedure was optimized, providing multiple copies of anti-sense RNA with reduced sample inputs: no more than 3 microg total RNA for one chip. Using the same RNA sample in all cases, the anti-sense RNA labeling method was compared with standard total RNA and mRNA methods by two sets of self-comparison experiments. Furthermore, the three methods in profiling analysis were compared with the same pair RNA samples. All the results indicated that the fidelity, reproducibility, and reliability showed no significant difference with conventional total RNA or mRNA microarrays.
    MeSH term(s) DNA-Directed RNA Polymerases/metabolism ; Gene Expression Profiling ; Nucleic Acid Amplification Techniques ; Oligonucleotide Array Sequence Analysis/methods ; RNA/analysis ; RNA, Messenger/analysis ; Viral Proteins
    Chemical Substances RNA, Messenger ; Viral Proteins ; RNA (63231-63-0) ; bacteriophage T7 RNA polymerase (EC 2.7.7.-) ; DNA-Directed RNA Polymerases (EC 2.7.7.6)
    Language Chinese
    Publishing date 2003-05
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2175256-4
    ISSN 1745-7270 ; 0582-9879 ; 1672-9145
    ISSN (online) 1745-7270
    ISSN 0582-9879 ; 1672-9145
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Systematic comparison of the fidelity of aRNA, mRNA and T-RNA on gene expression profiling using cDNA microarray.

    Li, Yao / Li, Tao / Liu, Sanzhen / Qiu, Minyan / Han, Zhiyong / Jiang, Zhenling / Li, Rongyu / Ying, Kang / Xie, Yi / Mao, Yumin

    Journal of biotechnology

    2003  Volume 107, Issue 1, Page(s) 19–28

    Abstract: In cDNA microarray technology, there are three main reverse transcription based RNA labeling methods, using total RNA (T-RNA), mRNA, and amplified antisense RNA (aRNA), respectively. However, despite the common use of the three types of RNAs, limited ... ...

    Abstract In cDNA microarray technology, there are three main reverse transcription based RNA labeling methods, using total RNA (T-RNA), mRNA, and amplified antisense RNA (aRNA), respectively. However, despite the common use of the three types of RNAs, limited data are available regarding their differences and concordances. In this report, we compared the three methods through two sets of self-comparison experiments using the same RNA sample in all cases. Within each method, duplicate hybridizations are highly reproducible with low biases, which are randomly produced. When combining different RNAs within a single array, correlation coefficients between the two channels are rather low, while the discrepancies are persistent. Furthermore, the fidelity of aRNA and mRNA microarrays in the expression profile study shows no significant difference with standard T-RNA based labeling methods. These results suggest that some RNA abundance are selectively changed during aRNA amplification/mRNA purification processes, but it will not affect the gene expression ratio of the two samples if the same type RNA are used. Therefore all three types of RNAs can be used in expression profiling analysis as long as the test and reference samples are generated by identical method within single study.
    MeSH term(s) Carcinoma, Hepatocellular/genetics ; Carcinoma, Hepatocellular/metabolism ; Gene Expression Profiling/methods ; Humans ; Liver/metabolism ; Liver Neoplasms/genetics ; Liver Neoplasms/metabolism ; Nucleic Acid Amplification Techniques ; Oligonucleotide Array Sequence Analysis/methods ; RNA/analysis ; RNA/genetics ; RNA, Antisense/analysis ; RNA, Antisense/genetics ; RNA, Messenger/analysis ; RNA, Messenger/genetics ; RNA, Transfer/analysis ; RNA, Transfer/genetics ; Reproducibility of Results ; Sensitivity and Specificity ; Staining and Labeling/methods
    Chemical Substances RNA, Antisense ; RNA, Messenger ; RNA (63231-63-0) ; RNA, Transfer (9014-25-9)
    Language English
    Publishing date 2003-11-05
    Publishing country Netherlands
    Document type Comparative Study ; Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't ; Validation Studies
    ZDB-ID 843647-2
    ISSN 1873-4863 ; 0168-1656 ; 1389-0352
    ISSN (online) 1873-4863
    ISSN 0168-1656 ; 1389-0352
    DOI 10.1016/j.jbiotec.2003.09.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2299: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top