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Article ; Online: Bacillus thuringiensis exopolysaccharides induced systemic resistance against Sclerotinia sclerotiorum in Brassica campestris L

Wang, Meiling / Geng, Lili / Jiao, Siming / Wang, Kui / Xu, Wenyue / Shu, Changlong / Zhang, Jie

Biological Control. 2023 Aug., v. 183 p.105267-

2023

Abstract: Bacillus thuringiensis is known as an entomopathogenic bacterium active against different agricultural pests. Recently, some novel functions have been explored, including plant growth-promoting activities. In our previous work, B. thuringiensis strains ... ...

Abstract	Bacillus thuringiensis is known as an entomopathogenic bacterium active against different agricultural pests. Recently, some novel functions have been explored, including plant growth-promoting activities. In our previous work, B. thuringiensis strains were found to effectively suppress Sclerotinia sclerotiorum by inducing systemic resistance (ISR) in Brassica campestris (L.). However, the mechanism by which exogenous signals from B. thuringiensis are perceived by plants is still not clear. Here, based on challenge-inoculation assays, we found that exopolysaccharides (EPSs) of B. thuringiensis strains could limit the spread of S. sclerotiorum by ISR in B. campestris. We purified and characterized the Bt 4F5 EPS, and the molecular weight was 66.2 kDa. The main monosaccharide units of Bt 4F5 EPS were determined to be mannose, GluA, glucose and galactose. Cellular defense response markers such as defense-related enzymes were induced after the 4F5 EPSs pretreatment in the leaves. Moreover, quantitative real-time PCR results indicated that the defense-related genes HEL and PDF1.2 were upregulated, suggesting an activation of the JA/ET signaling pathways by Bt 4F5 EPS. In addition, complete genome sequencing of the Bt 4F5 strain revealed twenty-one candidate genes involved in exopolysaccharide synthesis in chromosome. In summary, the EPSs of Bt strains play an important role in micro-associated molecular pattern (MAMP) perception during the process of Bt-triggered systemic resistance.
Keywords	Bacillus thuringiensis ; Brassica rapa subsp. oleifera ; Sclerotinia sclerotiorum ; biological control ; chromosomes ; entomopathogenic bacteria ; exopolysaccharides ; galactose ; genome ; glucose ; mannose ; molecular weight ; quantitative polymerase chain reaction ; Induced systemic resistance ; Extracellular polysaccharide ; Micro-associated molecular patterns ; Biocontrol of Sclerotinia sclerotiorum
Language	English
Dates of publication	2023-08
Publishing place	Elsevier Inc.
Document type	Article ; Online
ZDB-ID	1149971-0
ISSN	1049-9644
ISSN	1049-9644
DOI	10.1016/j.biocontrol.2023.105267
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Article ; Online: [Preparation, characterization and activity evaluation of

Sun, Ruijie / Xu, Tong / Liu, Yangyang / Zhang, Liming / Jiao, Siming / Zhang, Yuchen / Gao, Xiaodong / Wang, Zhuo / DU, Yuguang

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

2023 Volume 39, Issue 10, Page(s) 4135–4149

Abstract: The biofilms formed by pathogenic microorganisms seriously threaten human health and significantly enhance drug resistance, which urgently call for developing drugs specifically targeting on biofilms. Chitooligosaccharides extracted from shrimp and crab ... ...

Abstract	The biofilms formed by pathogenic microorganisms seriously threaten human health and significantly enhance drug resistance, which urgently call for developing drugs specifically targeting on biofilms. Chitooligosaccharides extracted from shrimp and crab shells are natural alkaline oligosaccharides with excellent antibacterial effects. Nevertheless, their inhibition efficacy on biofilms still needs to be improved.
MeSH term(s)	Humans ; Spirulina ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Chitosan/pharmacology ; Biofilms ; Chitin/pharmacology
Chemical Substances	oligochitosan ; Anti-Bacterial Agents ; Chitosan (9012-76-4) ; Chitin (1398-61-4)
Language	Chinese
Publishing date	2023-10-25
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1042206-7
ISSN	1872-2075 ; 1042-749X
ISSN (online)	1872-2075
ISSN	1042-749X
DOI	10.13345/j.cjb.230146
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Zs.A 3505: Show issues

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Article ; Online: Variations in metabolic enzymes cause differential changes of heparan sulfate and hyaluronan in high glucose treated cells on chip.

Wei, Jinhua / Liu, Dongdong / Xu, Tong / Zhu, Limeng / Jiao, Siming / Yuan, Xubing / Wang, Zhuo A / Li, Jianjun / Du, Yuguang

International journal of biological macromolecules

2023 Volume 253, Issue Pt 1, Page(s) 126627

Abstract: Glycocalyx dysfunction is believed as the first step in diabetic vascular disease. However, few studies have systematically investigated the influence of HG on the glycocalyx as a whole and its major constituent glycans towards one type of cell. ... ...

Abstract	Glycocalyx dysfunction is believed as the first step in diabetic vascular disease. However, few studies have systematically investigated the influence of HG on the glycocalyx as a whole and its major constituent glycans towards one type of cell. Furthermore, most studies utilized traditional two-dimensional (2D) cultures in vitro, which can't provide the necessary fluid environment for glycocalyx. Here, we utilized vascular glycocalyx on chips to evaluate the changes of glycocalyx and its constituent glycans in HG induced HUVECs. Fluorescence microscopy showed up-regulation of hyaluronan (HA) but down-regulation of heparan sulfate (HS). By analyzing the metabolic enzymes of both glycans, a decrease in the ratio of synthetic/degradative enzymes for HA and an increase in that for HS were demonstrated. Two substrates (UDP-GlcNAc, UDP-GlcA) for the synthesis of both glycans were increased according to omics analysis. Since they were firstly pumped into Golgi apparatus to synthesize HS, less substrates may be left for HA synthesis. Furthermore, the differential changes of HA and HS were confirmed in vessel slides from db/db mice. This study would deepen our understanding of impact of HG on glycocalyx formation and diabetic vascular disease.
MeSH term(s)	Mice ; Animals ; Hyaluronic Acid/metabolism ; Heparitin Sulfate/metabolism ; Diabetic Angiopathies ; Glucose ; Uridine Diphosphate
Chemical Substances	Hyaluronic Acid (9004-61-9) ; Heparitin Sulfate (9050-30-0) ; Glucose (IY9XDZ35W2) ; Uridine Diphosphate (58-98-0)
Language	English
Publishing date	2023-09-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.126627
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Article ; Online: Unveiling the inverse antimicrobial impact of a hetero-chitooligosaccharide on Candida tropicalis growth and biofilm formation.

Liu, Yangyang / Li, Ruilian / Zhang, Yuchen / Jiao, Siming / Xu, Tong / Zhou, Yuhang / Wang, Yujing / Wei, Jinhua / Du, Wei / Fujita, Morihisa / Du, Yuguang / Wang, Zhuo A

Carbohydrate polymers

2024 Volume 333, Page(s) 121999

Abstract: Chitosan and chitooligosaccharide (COS) are renowned for their potent antimicrobial prowess, yet the precise antimicrobial efficacy of COS remains elusive due to scant structural information about the utilized saccharides. This study delves into the ... ...

Abstract	Chitosan and chitooligosaccharide (COS) are renowned for their potent antimicrobial prowess, yet the precise antimicrobial efficacy of COS remains elusive due to scant structural information about the utilized saccharides. This study delves into the antimicrobial potential of COS, spotlighting a distinct hetero-chitooligosaccharide dubbed DACOS. In contrast to other COS, DACOS remarkably fosters the growth of Candida tropicalis planktonic cells and fungal biofilms. Employing gradient alcohol precipitation, DACOS was fractionated, unveiling diverse structural characteristics and differential impacts on C. tropicalis. Notably, in a murine model of systemic candidiasis, DACOS, particularly its 70 % alcohol precipitates, manifests a promotive effect on Candida infection. This research unveils a new pathway for exploring the intricate nexus between the structural attributes of chitosan oligosaccharides and their physiological repercussions, underscoring the imperative of crafting chitosan and COS with meticulously defined structural configurations.
MeSH term(s)	Animals ; Mice ; Candida tropicalis ; Chitosan/pharmacology ; Chitosan/chemistry ; Anti-Infective Agents ; Antifungal Agents/pharmacology ; Biofilms ; Oligosaccharides
Chemical Substances	oligochitosan ; Chitosan (9012-76-4) ; Anti-Infective Agents ; Antifungal Agents ; Oligosaccharides
Language	English
Publishing date	2024-02-28
Publishing country	England
Document type	Journal Article
ZDB-ID	1501516-6
ISSN	1879-1344 ; 0144-8617
ISSN (online)	1879-1344
ISSN	0144-8617
DOI	10.1016/j.carbpol.2024.121999
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Article ; Online: Characterization and application of active human α2,6-sialyltransferases ST6GalNAc V and ST6GalNAc VI recombined in Escherichia coli.

Pei, Caixia / Peng, Xinlv / Wu, Yiran / Jiao, Runmiao / Li, Tiehai / Jiao, Siming / Zhou, Lei / Li, Jianjun / Du, Yuguang / Qian, Eika W

Enzyme and microbial technology

2024 Volume 177, Page(s) 110426

Abstract: Eukaryotic sialyltransferases play key roles in many physiological and pathological events. The expression of active human recombinant sialyltransferases in bacteria is still challenging. In the current study, the genes encoding human N- ... ...

Abstract	Eukaryotic sialyltransferases play key roles in many physiological and pathological events. The expression of active human recombinant sialyltransferases in bacteria is still challenging. In the current study, the genes encoding human N-acetylgalactosaminide α2,6-sialyltransferase V (hST6GalNAc V) and N-acetylgalactosaminide α2,6-sialyltransferase VI (hST6GalNAc VI) lacking the N-terminal transmembrane domains were cloned into the expression vectors, pET-32a and pET-22b, respectively. Soluble and active forms of recombinant hST6GalNAc V and hST6GalNAc VI when coexpressed with the chaperone plasmid pGro7 were successfully achieved in Escherichia coli. Further, lactose (Lac), Lacto-N-triose II (LNT II), lacto-N-tetraose (LNT), and sialyllacto-N-tetraose a (LSTa) were used as acceptor substrates to investigate their activities and substrate specificities. Unexpectedly, both can transfer sialic acid onto all those substrates. Compared with hST6GalNAc V expressed in the mammalian cells, the recombinant two α2,6-sialyltransferases in bacteria displayed flexible substrate specificities and lower enzymatic efficiency. In addition, an important human milk oligosaccharide disialyllacto-N-tetraose (DSLNT) can be synthesized by both human α2,6-sialyltransferases expressed in E. coli using LSTa as an acceptor substrate. To the best of our knowledge, these two active human α2,6-sialyltransferases enzymes were expressed in bacteria for the first time. They showed a high potential to be applied in biotechnology and investigating the molecular mechanisms of biological and pathological interactions related to sialylated glycoconjugates.
MeSH term(s)	Humans ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Sialyltransferases/genetics ; Sialyltransferases/metabolism ; Recombinant Proteins/metabolism ; Recombinant Proteins/genetics ; Lactose/metabolism ; Substrate Specificity ; Oligosaccharides/metabolism ; Cloning, Molecular ; Animals
Chemical Substances	Sialyltransferases (EC 2.4.99.-) ; Recombinant Proteins ; Lactose (J2B2A4N98G) ; Oligosaccharides ; lacto-N-neotetraose (BY63N40B1L)
Language	English
Publishing date	2024-03-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	423729-8
ISSN	1879-0909 ; 0141-0229
ISSN (online)	1879-0909
ISSN	0141-0229
DOI	10.1016/j.enzmictec.2024.110426
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Article: Glucosamine Ameliorates Symptoms of High-Fat Diet-Fed Mice by Reversing Imbalanced Gut Microbiota.

Yuan, Xubing / Zheng, Junping / Ren, Lishi / Jiao, Siming / Feng, Cui / Du, Yuguang / Liu, Hongtao

Frontiers in pharmacology

2021 Volume 12, Page(s) 694107

Abstract: Glucosamine (GlcN) is used as a supplement for arthritis and joint pain and has been proved to have effects on inflammation, cancer, and cardiovascular diseases. However, there are limited studies on the regulatory mechanism of GlcN against glucose and ... ...

Abstract	Glucosamine (GlcN) is used as a supplement for arthritis and joint pain and has been proved to have effects on inflammation, cancer, and cardiovascular diseases. However, there are limited studies on the regulatory mechanism of GlcN against glucose and lipid metabolism disorder. In this study, we treated high-fat diet (HFD)-induced diabetic mice with GlcN (1 mg/ml, in drinking water) for five months. The results show that GlcN significantly reduced the fasting blood glucose of HFD-fed mice and improved glucose tolerance. The feces of intestinal contents in mice were analyzed using 16s rDNA sequencing. It was indicated that GlcN reversed the imbalanced gut microbiota in HFD-fed mice. Based on the PICRUSt assay, the signaling pathways of glucolipid metabolism and biosynthesis were changed in mice with HFD feeding. By quantitative real-time PCR (qPCR) and hematoxylin and eosin (H&E) staining, it was demonstrated that GlcN not only inhibited the inflammatory responses of colon and white adipose tissues, but also improved the intestinal barrier damage of HFD-fed mice. Finally, the correlation analysis suggests the most significantly changed intestinal bacteria were positively or negatively related to the occurrence of inflammation in the colon and fat tissues of HFD-fed mice. In summary, our studies provide a theoretical basis for the potential application of GlcN to glucolipid metabolism disorder through the regulation of gut microbiota.
Language	English
Publishing date	2021-06-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2021.694107
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Article: Exploring Effects of Chitosan Oligosaccharides on Mice Gut Microbiota in

Zhang, Chen / Jiao, Siming / Wang, Zhuo A / Du, Yuguang

Frontiers in microbiology

2018 Volume 9, Page(s) 2388

Abstract: Chitosan oligosaccharides (COS) have shown positive effects on host gut health and influence on intestinal microbial community. However, the bioactivity and mechanism of COS on gut microbiota is still poorly understood. Here, we presented systematic ... ...

Abstract	Chitosan oligosaccharides (COS) have shown positive effects on host gut health and influence on intestinal microbial community. However, the bioactivity and mechanism of COS on gut microbiota is still poorly understood. Here, we presented systematic studies of COS on mice fecal/gut microbiota. During
Language	English
Publishing date	2018-10-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2018.02388
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: A review on the preparation of chitosan oligosaccharides and application to human health, animal husbandry and agricultural production

Yuan, Xubing / Cheng, Gong / Du, Yuguang / Feng, Cui / Jiao, Siming / Liu, Hongtao / Zheng, Junping

Carbohydrate polymers. 2019 Sept. 15, v. 220

2019

Abstract: Chitosan oligosaccharides (COS) are the degraded products of chitin or chitosan prepared by chemical or enzymatic hydrolysis. As compared to chitosan, COS not only exhibit some specific physicochemical properties such as excellent water solubility, ... ...

Abstract	Chitosan oligosaccharides (COS) are the degraded products of chitin or chitosan prepared by chemical or enzymatic hydrolysis. As compared to chitosan, COS not only exhibit some specific physicochemical properties such as excellent water solubility, biodegradability and biocompatibility, but also have a variety of functionally biological activities including anti-inflammation, anti-bacteria, immunomodulation, neuroprotection and so on. This review aims to summarize the preparation and structural characterization methods of COS, and will discuss the application of COS or their derivatives to human health, animal husbandry and agricultural production. COS have been demonstrated to prevent the occurrence of human health-related diseases, enhance the resistance to diseases of livestock and poultry, and improve the growth and quality of crops in plant cultivation. Overall, COS have presented a broad developmental potential and application prospect in the healthy field that deserves further exploration.
Keywords	animal husbandry ; biocompatibility ; biodegradability ; chitin ; chitosan ; crops ; disease resistance ; enzymatic hydrolysis ; human health ; humans ; immunomodulation ; livestock ; livestock diseases ; neuroprotective effect ; oligosaccharides ; water solubility
Language	English
Dates of publication	2019-0915
Size	p. 60-70.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1501516-6
ISSN	1879-1344 ; 0144-8617
ISSN (online)	1879-1344
ISSN	0144-8617
DOI	10.1016/j.carbpol.2019.05.050
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Article ; Online: Overexpression and biochemical characterization of a truncated endo-α (1 → 3)-fucoidanase from alteromonas sp. SN-1009.

Zhu, Chenlu / Liu, Zebin / Ren, Lishi / Jiao, Siming / Zhang, Xuebing / Wang, Qiukuan / Li, Zhimin / Du, Yuguang / Li, Jian-Jun

Food chemistry

2021 Volume 353, Page(s) 129460

Abstract: Endo-fucoidanases are important in structural analysis of fucoidans and preparation of fuco-oligosaccharides. However their enzymological properties and analysis of degradation products are scarcely investigated. Truncated endo-α (1 → 3)-fucoidanase Fda1 ...

Abstract	Endo-fucoidanases are important in structural analysis of fucoidans and preparation of fuco-oligosaccharides. However their enzymological properties and analysis of degradation products are scarcely investigated. Truncated endo-α (1 → 3)-fucoidanase Fda1 (tFda1B from Alteromonas sp. was overexpressed and characterized, showing highest activity at pH 7.0, 35 °C, and 1.0 M NaCl. Its K
MeSH term(s)	Alteromonas/enzymology ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Catalytic Domain ; Enzyme Stability ; Escherichia coli/genetics ; Gene Expression Regulation, Bacterial ; Hydrogen-Ion Concentration ; Hydrolases/chemistry ; Hydrolases/genetics ; Hydrolases/metabolism ; Mutagenesis, Site-Directed ; Oligosaccharides/chemistry ; Phaeophyceae/chemistry ; Phaeophyceae/metabolism ; Phylogeny ; Polysaccharides/chemistry ; Polysaccharides/metabolism ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Spectrometry, Mass, Electrospray Ionization ; Substrate Specificity ; Tandem Mass Spectrometry
Chemical Substances	Bacterial Proteins ; Oligosaccharides ; Polysaccharides ; Recombinant Proteins ; fucoidan (9072-19-9) ; Hydrolases (EC 3.-)
Language	English
Publishing date	2021-03-07
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2021.129460
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Article: Blood-Brain Barrier Permeable Chitosan Oligosaccharides Interfere with β-Amyloid Aggregation and Alleviate β-Amyloid Protein Mediated Neurotoxicity and Neuroinflammation in a Dose- and Degree of Polymerization-Dependent Manner

Zhu, Limeng / Li, Ruilian / Jiao, Siming / Wei, Jinhua / Yan, Yalu / Wang, Zhuo A / Li, Jianjun / Du, Yuguang

Marine drugs. 2020 Sept. 25, v. 18, no. 10

2020

Abstract: It is proven that β-amyloid (Aβ) aggregates containing cross-β-sheet structures led to oxidative stress, neuroinflammation, and neuronal loss via multiple pathways. Therefore, reduction of Aβ neurotoxicity via inhibiting aggregation of Aβ or dissociating ...

Abstract	It is proven that β-amyloid (Aβ) aggregates containing cross-β-sheet structures led to oxidative stress, neuroinflammation, and neuronal loss via multiple pathways. Therefore, reduction of Aβ neurotoxicity via inhibiting aggregation of Aβ or dissociating toxic Aβ aggregates into nontoxic forms might be effective therapeutic methods for Alzheimer’s disease (AD) treatment. This study was designed to explore interference of chitosan oligosaccharides (COS) on β-(1-42)-amyloid protein (Aβ42) aggregation and Aβ42-induced cytotoxicity. Here it was demonstrated that COS showed good blood-brain barrier (BBB) penetration ability in vitro and in vivo. The experimental results showed that COS efficiently interfered with Aβ42 aggregation in dose- and degree of polymerization (DP)-dependent manners, and COS monomer with DP6 showed the best effect on preventing conformational transition into β-sheet-rich structures. Based on the binding affinity analysis by microscale thermophoresis (MST), it was confirmed that COS could directly bind with Aβ42 in a DP-dependent manner. Our findings demonstrated that different performance of COS monomers with different DPs against Aβ42 assembly was, to some extent, attributable to their different binding capacities with Aβ42. As a result, COS significantly ameliorated Aβ42-induced cytotoxicity. Taken together, our studies would point towards a potential role of COS in treatment of AD.
Keywords	Alzheimer disease ; binding capacity ; blood-brain barrier ; chitosan ; cytotoxicity ; drugs ; methodology ; neurons ; neurotoxicity ; oligosaccharides ; oxidative stress ; polymerization ; therapeutics
Language	English
Dates of publication	2020-0925
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-light
ZDB-ID	2175190-0
ISSN	1660-3397
ISSN	1660-3397
DOI	10.3390/md18100488
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