Article ; Online: Photon counting imaging with an electron-bombarded CCD: towards a parallel-processing photoelectronic time-to-amplitude converter.
The Review of scientific instruments
2014 Volume 85, Issue 12, Page(s) 123102
Abstract: We have used an electron-bombarded CCD for optical photon counting imaging. The photon event pulse height distribution was found to be linearly dependent on the gain voltage. We propose on this basis that a gain voltage sweep during exposure in an ... ...
Abstract | We have used an electron-bombarded CCD for optical photon counting imaging. The photon event pulse height distribution was found to be linearly dependent on the gain voltage. We propose on this basis that a gain voltage sweep during exposure in an electron-bombarded sensor would allow photon arrival time determination with sub-frame exposure time resolution. This effectively uses an electron-bombarded sensor as a parallel-processing photoelectronic time-to-amplitude converter, or a two-dimensional photon counting streak camera. Several applications that require timing of photon arrival, including Fluorescence Lifetime Imaging Microscopy, may benefit from such an approach. A simulation of a voltage sweep performed with experimental data collected with different acceleration voltages validates the principle of this approach. Moreover, photon event centroiding was performed and a hybrid 50% Gaussian/Centre of Gravity + 50% Hyperbolic cosine centroiding algorithm was found to yield the lowest fixed pattern noise. Finally, the camera was mounted on a fluorescence microscope to image F-actin filaments stained with the fluorescent dye Alexa 488 in fixed cells. |
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Language | English |
Publishing date | 2014-12 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 209865-9 |
ISSN | 1089-7623 ; 0034-6748 |
ISSN (online) | 1089-7623 |
ISSN | 0034-6748 |
DOI | 10.1063/1.4901935 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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