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Article ; Online: Network pharmacology-based mechanism prediction and pharmacological validation of Bushenhuoxue formula attenuating postmenopausal osteoporosis in ovariectomized mice.

Xia, Chenjie / Zhu, Haowei / Li, Jin / Jin, Hongting / Fu, Danqing

Journal of orthopaedic surgery and research

2023 Volume 18, Issue 1, Page(s) 200

Abstract: Background: Bushenhuoxue (BSHX) formula, a ten-compound herbal decoction, is widely used to treat postmenopausal osteoporosis (PMOP) in China. However, the mechanism is not clear yet.: Methods: The underlying biological processes and signaling ... ...

Abstract	Background: Bushenhuoxue (BSHX) formula, a ten-compound herbal decoction, is widely used to treat postmenopausal osteoporosis (PMOP) in China. However, the mechanism is not clear yet. Methods: The underlying biological processes and signaling pathways were predicted by network pharmacology. In vivo experimental study, 24 female C57BL/6 J mice were randomly divided into sham, ovariectomized (OVX) and BSHX formula groups. Mice in the latter two groups were subjected to bilateral ovariectomy, and mice in the BSHX formula group were extra treated by BSHX formula at an oral dosage of 0.2 mL/10 g for 8 weeks. The femur samples were harvested for tissue analyses including μCT assay, histology and immunohistochemical (IHC) staining of VEGF signaling. Results: A total of 218 active ingredients and 274 related targets were identified in BSHX formula. After matching with 292 targets of PMOP, 64 overlapping genes were obtained. GO and KEGG enrichment analyses on these 64 genes revealed that angiogenesis and VEGF signaling were considered as the potential therapeutic mechanism of BSHX formula against PMOP. Animal experiments showed that mice in the BSHX formula-treated group presented increased bone mass, microstructural parameters, blood vessel numbers and an activation of VEGF signaling (VEGF, COX2, eNOS and CD31) compared to the OVX mice. Conclusion: This study revealed that BSHX formula exerts anti-PMOP effects possibly through activating VEGF signaling-mediated angiogenesis.
MeSH term(s)	Humans ; Mice ; Female ; Animals ; Osteoporosis, Postmenopausal/drug therapy ; Osteoporosis, Postmenopausal/metabolism ; Vascular Endothelial Growth Factor A ; Network Pharmacology ; Mice, Inbred C57BL ; Signal Transduction ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Ovariectomy
Chemical Substances	Vascular Endothelial Growth Factor A ; Drugs, Chinese Herbal
Language	English
Publishing date	2023-03-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2252548-8
ISSN	1749-799X ; 1749-799X
ISSN (online)	1749-799X
ISSN	1749-799X
DOI	10.1186/s13018-023-03696-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Study of the curative effect of Zhang's Xibi formula and its underlying mechanism involving inhibition of inflammatory responses and delay of knee osteoarthritis.

Sun, Qi / Jin, Hongting / Li, Wuyin / Tong, Peijian / Yuan, Wenhua

Journal of orthopaedic surgery and research

2023 Volume 18, Issue 1, Page(s) 963

Abstract: Objective: To verify the clinical efficacy of Zhang's Xibi formula (ZSXBF) and explain the mechanism underlying its therapeutic effect.: Methods: Preliminary elucidation of the clinical efficacy of ZSXBF in treating KOA in self-control studies, ... ...

Abstract	Objective: To verify the clinical efficacy of Zhang's Xibi formula (ZSXBF) and explain the mechanism underlying its therapeutic effect. Methods: Preliminary elucidation of the clinical efficacy of ZSXBF in treating KOA in self-control studies, exploration of its mechanism of action with network pharmacology methods, and validation in animal experiments. Results: In clinical studies, ZSXBF administration effectively improved patient quality of life and reduce pain. Network pharmacology was used to explore the possible mechanisms underlying its treatment effect, and after verification in clinical experience and animal experiments, it was found that ZSXBF regulated the expression of immune-related proteins such as IL-17, ERK1, and TP53 in mouse knee joints. Conclusion: ZSXBF, which is a traditional Chinese medicine compound that is used to clear heat and detoxify, can effectively improve the clinical symptoms of KOA patients, and its underlying mechanism includes the regulation of human immune-related proteins.
MeSH term(s)	Humans ; Animals ; Mice ; Osteoarthritis, Knee/drug therapy ; Quality of Life ; Knee Joint ; Hot Temperature ; Medicine, Chinese Traditional ; Drugs, Chinese Herbal
Chemical Substances	Drugs, Chinese Herbal
Language	English
Publishing date	2023-12-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2252548-8
ISSN	1749-799X ; 1749-799X
ISSN (online)	1749-799X
ISSN	1749-799X
DOI	10.1186/s13018-023-04453-6
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Article ; Online: Protective effects of Shensuitongzhi formula on intervertebral disc degeneration via downregulation of NF-κB signaling pathway and inflammatory response.

Wang, Xu / Zeng, Qinghe / Ge, Qinwen / Hu, Songfeng / Jin, Hongting / Wang, Ping-Er / Li, Ju

Journal of orthopaedic surgery and research

2024 Volume 19, Issue 1, Page(s) 80

Abstract: Low back pain (LBP) is a common orthopedic disease over the world. Lumbar intervertebral disc degeneration (IDD) is regarded as an important cause of LBP. Shensuitongzhi formula (SSTZF) is a drug used in clinical treatment for orthopedic diseases. It has ...

Abstract	Low back pain (LBP) is a common orthopedic disease over the world. Lumbar intervertebral disc degeneration (IDD) is regarded as an important cause of LBP. Shensuitongzhi formula (SSTZF) is a drug used in clinical treatment for orthopedic diseases. It has been found that SSTZF can have a good treatment for IDD. But the exact mechanism has not been clarified. The results showed that SSTZF protects against LSI-induced degeneration of cartilage endplates and intervertebral discs. Meanwhile, SSTZF treatment dramatically reduces the expression of inflammatory factor as well as the expression of catabolism protein and upregulates the expression of anabolism protein in LSI-induced mice. In addition, SSTZF delayed the progression of LSI-induced IDD via downregulation the level of NF-κB signaling key gene RELA and phosphorylation of key protein P65 in endplate chondrocytes. Our study has illustrated the treatment as well as the latent mechanism of SSTZF in IDD.
MeSH term(s)	Mice ; Animals ; NF-kappa B/metabolism ; Intervertebral Disc Degeneration/genetics ; Down-Regulation ; Signal Transduction ; Intervertebral Disc/metabolism
Chemical Substances	NF-kappa B
Language	English
Publishing date	2024-01-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2252548-8
ISSN	1749-799X ; 1749-799X
ISSN (online)	1749-799X
ISSN	1749-799X
DOI	10.1186/s13018-023-04391-3
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Article ; Online: Dnmt3b ablation affects fracture repair process by regulating apoptosis.

Wang, Xu / Ge, Qinwen / Zeng, Qinghe / Zou, Kaiao / Bao, Zhengsheng / Ying, Jun / Wu, Zhen / Jin, Hongting / Chen, Jiali / Xu, Taotao

BMC musculoskeletal disorders

2024 Volume 25, Issue 1, Page(s) 180

Abstract: Purpose: Previous studies have shown that DNA methyltransferase 3b (Dnmt3b) is the only Dnmt responsive to fracture repair and Dnmt3b ablation in Prx1-positive stem cells and chondrocyte cells both delayed fracture repair. Our study aims to explore the ... ...

Abstract	Purpose: Previous studies have shown that DNA methyltransferase 3b (Dnmt3b) is the only Dnmt responsive to fracture repair and Dnmt3b ablation in Prx1-positive stem cells and chondrocyte cells both delayed fracture repair. Our study aims to explore the influence of Dnmt3b ablation in Gli1-positive stem cells in fracture healing mice and the underlying mechanism. Methods: We generated Gli1-CreERT2; Dnmt3bflox/flox (Dnmt3b Results: The cartilaginous callus significantly decrease in ablation of Dnmt3b in Gli1-positive stem cells during fracture repair. The chondrogenic and osteogenic indicators (Sox9 and Runx2) in the fracture healing tissues in Dnmt3b Conclusions: Our study provides an evidence that Dnmt3b ablation Gli1-positive stem cells can affect fracture healing and lead to poor fracture healing by regulating apoptosis to decrease chondrocyte hypertrophic maturation.
MeSH term(s)	Animals ; Mice ; Apoptosis ; Bony Callus/pathology ; Fracture Healing/physiology ; Tibial Fractures/surgery ; Zinc Finger Protein GLI1
Chemical Substances	Zinc Finger Protein GLI1 ; Dnmt3b protein, mouse (EC 2.1.1.-)
Language	English
Publishing date	2024-02-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2041355-5
ISSN	1471-2474 ; 1471-2474
ISSN (online)	1471-2474
ISSN	1471-2474
DOI	10.1186/s12891-024-07283-7
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Article ; Online: Catalpol promotes articular cartilage repair by enhancing the recruitment of endogenous mesenchymal stem cells.

Wu, Congzi / Shi, Zhenyu / Ge, Qinwen / Xu, HuiHui / Wu, Zhen / Tong, Peijian / Jin, Hongting

Journal of cellular and molecular medicine

2024 Volume 28, Issue 7, Page(s) e18242

Abstract: Articular cartilage defect is challenged by insufficient regenerative ability of cartilage. Catalpol (CA), the primary active component of Rehmanniae Radix, could exert protective effects against various diseases. However, the impact of CA on the ... ...

Abstract	Articular cartilage defect is challenged by insufficient regenerative ability of cartilage. Catalpol (CA), the primary active component of Rehmanniae Radix, could exert protective effects against various diseases. However, the impact of CA on the treatment of articular cartilage injuries is still unclear. In this study, full-thickness articular cartilage defect was induced in a mouse model via surgery. The animals were intraperitoneally injected with CA for 4 or 8 weeks. According to the results of macroscopic observation, micro-computed tomography CT (μCT), histological and immunohistochemistry staining, CA treatment could promote mouse cartilage repair, resulting in cartilage regeneration, bone structure improvement and matrix anabolism. Specifically, an increase in the expression of CD90, the marker of mesenchymal stem cells (MSCs), in the cartilage was observed. In addition, we evaluated the migratory and chondrogenic effects of CA on MSCs. Different concentration of CA was added to C3H10 T1/2 cells. The results showed that CA enhanced cell migration and chondrogenesis without affecting proliferation. Collectively, our findings indicate that CA may be effective for the treatment of cartilage defects via stimulation of endogenous MSCs.
MeSH term(s)	Animals ; Mice ; Cartilage, Articular/pathology ; X-Ray Microtomography ; Cell Differentiation ; Cartilage Diseases/metabolism ; Mesenchymal Stem Cells ; Mesenchymal Stem Cell Transplantation/methods ; Chondrogenesis ; Iridoid Glucosides
Chemical Substances	catalpol (2415-24-9) ; Iridoid Glucosides
Language	English
Publishing date	2024-03-20
Publishing country	England
Document type	Journal Article
ZDB-ID	2074559-X
ISSN	1582-4934 ; 1582-4934 ; 1582-1838
ISSN (online)	1582-4934
ISSN	1582-4934 ; 1582-1838
DOI	10.1111/jcmm.18242
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Article ; Online: Novel nomograms for predicting the risk of low distal bone strength: development and validation in a Chinese population-based observational study.

Wu, Congzi / Liu, Ting / Shi, Zhenyu / Fang, Liang / Jin, Hongting / Tong, Peijian

Journal of orthopaedic surgery and research

2023 Volume 18, Issue 1, Page(s) 70

Abstract: Background: This study aims to develop nomogram models based on the speed of sound (SOS) measurements results along with demographic information to predict the risk of low bone strength (LBS) of radius appropriate to the Chinese population of a broad ... ...

Abstract	Background: This study aims to develop nomogram models based on the speed of sound (SOS) measurements results along with demographic information to predict the risk of low bone strength (LBS) of radius appropriate to the Chinese population of a broad age spectrum. Methods: A population-based cross-sectional study was conducted in 5 outpatient clinics located in Zhejiang, the southern part of China. A total of 38,699 participants from 2013 to 2017 were included. Baseline measurements included SOS of the distal radius and clinical risk factor evaluation. Logistic regression models were used to evaluate prognosis and identify independent predictive factors, which were then utilized to establish nomograms for predicting the low bone strength of radius. The discrimination and calibration of nomograms were validated using the calibration plots, the decision curve analysis (DCA), and the receiver operating characteristics curve (ROC). Results: A total of 19,845 of the 38,904 participants ranged in age from 10 to 88 years were selected in this process. LBP nomogram model 1 was constructed based on age, weight, height, BMI, and gender. LBP nomogram model 2 was constructed based on age, height, BMI, and gender. The AUCs for model 1 and model 2 were 0.838 (95% CI: 0.832-0.844) and 0.837 (95% CI: 0.831-0.843), respectively. High-quality calibration plots and DCA in nomogram models were noticed, indicated that the constructed nomogram models were clinically useful. Conclusions: Our study demonstrates that the nomograms established in this study could effectively evaluate the high-risk population groups of distal radius fracture in China.
MeSH term(s)	Humans ; Child ; Adolescent ; Young Adult ; Adult ; Middle Aged ; Aged ; Aged, 80 and over ; Nomograms ; Cross-Sectional Studies ; East Asian People ; Ambulatory Care Facilities ; Area Under Curve ; ROC Curve ; Prognosis ; Retrospective Studies
Language	English
Publishing date	2023-01-30
Publishing country	England
Document type	Observational Study ; Journal Article
ZDB-ID	2252548-8
ISSN	1749-799X ; 1749-799X
ISSN (online)	1749-799X
ISSN	1749-799X
DOI	10.1186/s13018-023-03546-6
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Article ; Online: Osteosclerosis and osteoporosis - different bone impairments from high bone turnover of skeletal fluorosis: a case study on the femoral head.

Fang, Liang / Xia, Chenjie / Li, Shaoguang / Xiao, Luwei / Jin, Hongting

Pathology

2021

Language	English
Publishing date	2021-04-03
Publishing country	England
Document type	Letter
ZDB-ID	7085-3
ISSN	1465-3931 ; 0031-3025
ISSN (online)	1465-3931
ISSN	0031-3025
DOI	10.1016/j.pathol.2020.11.014
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Article: Case Report: First Documented Hip Arthroplasty on Chinese Patient with Ochronotic Arthropathy.

Ying, Zhibo / Lu, Jufeng / Wang, Xucheng / Zeng, Qinghe / Jin, Hongting / He, Bangjian

Frontiers in surgery

2022 Volume 9, Page(s) 875777

Abstract: Alkaptonuria (AKU) is a rare autosomal recessive disorder caused by homogentisc acid (HGA) accumulation, the deposition of which in the joints usually causes ochronotic arthropathy. With no specific therapy for AKU currently, total joint arthroplasty in ... ...

Abstract	Alkaptonuria (AKU) is a rare autosomal recessive disorder caused by homogentisc acid (HGA) accumulation, the deposition of which in the joints usually causes ochronotic arthropathy. With no specific therapy for AKU currently, total joint arthroplasty in ochronotic arthropathy is applied to relieve the symptoms. A 63-year-old female patient came to our Orthopedic Surgery Department in 2019, complaining of severe limitation of movement and pain in the right hip for more than one year. A right total hip arthroplasy (THA) was performed due to the ineffective conservative therapy. At a follow-up of more than 15 months, the woman had full mobility with no complaining of pains. Since there is no relevant case reported about THA therapy for Chinese AKU patients, this report provides a feasible scheme, which makes clinical data more comprehensive.
Language	English
Publishing date	2022-05-09
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2773823-1
ISSN	2296-875X
ISSN	2296-875X
DOI	10.3389/fsurg.2022.875777
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Article: Disruption of Col9a2 expression leads to defects in osteochondral homeostasis and osteoarthritis-like phenotype in mice.

Dong, Rui / Xu, Huihui / Wang, Pinger / Fang, Liang / Xiao, Luwei / Lv, Shuaijie / Tong, Peijian / Jin, Hongting

Journal of orthopaedic translation

2023 Volume 41, Page(s) 33–41

Abstract: Background/objective: As one of the branched chains of Type IX collagen (Col9), Collagen IX alpha2 (Col9a2) has been reported to be associated with several orthopedic conditions. However, the relationship between Col9a2 and knee osteoarthritis (KOA) ... ...

Abstract	Background/objective: As one of the branched chains of Type IX collagen (Col9), Collagen IX alpha2 (Col9a2) has been reported to be associated with several orthopedic conditions. However, the relationship between Col9a2 and knee osteoarthritis (KOA) remains to be elucidated. Methods: To probe the relationship between Col9a2 and KOA, we performed a systematic analysis of Col9a2-deficient (Col9a2 Results: We found that the subchondral bone (SCB) in the knee joint of Col9a2 Conclusion: Overall, Col9a2 is essential for maintaining osteochondral homeostasis in the knee joint of mice, and the absence of this gene is accompanied by distinct sclerosis of the SCB and a reduction in load-bearing capacity; in the late stage, in the lack of SCB stress inhibition, excessive load is consistently exerted on the cartilage, ultimately leading to osteoarthritic-like articular cartilage damage.
Language	English
Publishing date	2023-08-14
Publishing country	Singapore
Document type	Journal Article
ZDB-ID	2747531-1
ISSN	2214-031X
ISSN	2214-031X
DOI	10.1016/j.jot.2023.08.005
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Article ; Online: Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH.

Xu, Huihui / Zeng, Qinghe / Zou, Kaiao / Huang, Haipeng / Chen, Jiali / Wang, Pinger / Yuan, Wenhua / Xiao, Luwei / Tong, Peijian / Jin, Hongting

Apoptosis : an international journal on programmed cell death

2023 Volume 28, Issue 9-10, Page(s) 1332–1345

Abstract: Background: This study aimed to investigate the pathogenic factors of glucocorticoids (GCs)-induced osteonecrosis of the femoral head (GONFH) and its underlying pathogenesis in vivo and in vitro.: Methods: Radiographical (µCT) scanning, ... ...

Abstract	Background: This study aimed to investigate the pathogenic factors of glucocorticoids (GCs)-induced osteonecrosis of the femoral head (GONFH) and its underlying pathogenesis in vivo and in vitro. Methods: Radiographical (µCT) scanning, histopathological, immunohistochemical, reactive oxygen species (ROS) and tunel staining were conducted on GONFH patients and rats. ROS, tunel, flow cytometry, alkaline phosphatase, Oil red O staining, reverse transcription‑quantitative PCR and western blotting were applied to elucidate the exact pathogenesis mechanism. Results: Clinical and animal studies demonstrated increased levels of ROS, aggravated oxidative stress (OS) microenvironment, augmented apoptosis and imbalance in osteogenic/lipogenic in the GONFH group compared to the control group. The fate of mesenchymal stem cells (MSCs) directed by GCs is a crucial factor in determining GONFH. In vitro studies further revealed that GCs promote excessive ROS production through the expression of NOX family proteins, leading to a deterioration of the OS microenvironment in MSCs, ultimately resulting in apoptosis and imbalance in osteogenic/lipogenic differentiation. Furthermore, our results confirmed that the NOX inhibitor-diphenyleneiodonium chloride and the NF-κB inhibitor-BAY 11-7082 ameliorated apoptosis and osteogenic/lipogenic differentiation imbalance of MSCs induced by an excess of GCs. Conclusion: We demonstrated for the first time that the aggravation of the OS microenvironment in MSCs caused by high doses of GCs leading to apoptosis and differentiation imbalance is a crucial factor in the pathogenesis of GONFH, mediated through activating the NOX/ROS/NF-κB signaling pathway.
MeSH term(s)	Humans ; Rats ; Animals ; NF-kappa B/genetics ; NF-kappa B/metabolism ; Reactive Oxygen Species/metabolism ; Glucocorticoids/adverse effects ; Glucocorticoids/metabolism ; Apoptosis ; Signal Transduction ; Mesenchymal Stem Cells
Chemical Substances	NF-kappa B ; Reactive Oxygen Species ; Glucocorticoids
Language	English
Publishing date	2023-06-12
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1452360-7
ISSN	1573-675X ; 1360-8185
ISSN (online)	1573-675X
ISSN	1360-8185
DOI	10.1007/s10495-023-01860-2
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