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  1. Article ; Online: Naturally occurring quercetin and myricetin as potent inhibitors for human ectonucleotide pyrophosphatase/phosphodiesterase 1

    Peeradon Duangiad / Bodee Nutho / Thawatchai Chaijarasphong / Noppawan Phumala Morales / Thunyarat Pongtharangkul / Itaru Hamachi / Akio Ojida / Jirarut Wongkongkatep

    Scientific Reports, Vol 14, Iss 1, Pp 1-

    2024  Volume 13

    Abstract: Abstract Ecto-nucleotide pyrophosphatases/phosphodiesterases 1 (ENPP1) is a key enzyme in purinergic signaling pathways responsible for cell-to-cell communications and regulation of several fundamental pathophysiological processes. In this study, Kyoto ... ...

    Abstract Abstract Ecto-nucleotide pyrophosphatases/phosphodiesterases 1 (ENPP1) is a key enzyme in purinergic signaling pathways responsible for cell-to-cell communications and regulation of several fundamental pathophysiological processes. In this study, Kyoto Green, a rapid chemical sensor of pyrophosphate, was employed to screen for effective ENPP1 inhibitors among five representative flavonoids (quercetin, myricetin, morin, kaempferol, and quercetin-3-glucoside), five nucleosides (adenosine, guanosine, inosine, uridine, and cytidine), and five deoxynucleosides (2′- and 3′-deoxyadenosine, 2′-deoxyguanosine, 2′-deoxyinosine, and 2′-deoxyuridine). Conventional colorimetric, fluorescence, and bioluminescence assays revealed that ENPP1 was effectively inhibited by quercetin (K i ~ 4 nM) and myricetin (K i ~ 32 nM) when ATP was used as a substrate at pH 7.4. In silico analysis indicated that the presence of a chromone scaffold, particularly one containing a hydroxyl group at the 3′ position on the B ring, may promote binding to the active site pocket of ENPP1 and enhance inhibition. This study demonstrated that the naturally derived quercetin and myricetin could effectively inhibit ENPP1 enzymatic activity and may offer health benefits in arthritis management.
    Keywords Medicine ; R ; Science ; Q
    Subject code 572
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Viable but nonculturable state of Escherichia coli, Pseudomonas putida and Lactococcus lactis during exposure to toxic chemicals, as revealed by headspace gas chromatography and indirect conductivity techniques

    Panwajee Payongsri / Nattapat Charoenrat / Thunyarat Pongtharangkul / Jirarut Wongkongkatep

    Songklanakarin Journal of Science and Technology (SJST), Vol 42, Iss 6, Pp 1164-

    2020  Volume 1170

    Abstract: The bioconversion of water-immiscible chemicals by microbes may occur through viable but nonculturable cells (VBNC) and the evaluation of such cellular activity is important for a deeper understanding of the metabolic process. In this study, the ... ...

    Abstract The bioconversion of water-immiscible chemicals by microbes may occur through viable but nonculturable cells (VBNC) and the evaluation of such cellular activity is important for a deeper understanding of the metabolic process. In this study, the metabolic CO2 production in a bacteria interface emulsion, consisting of bacteria, chitosan and solvent, has been monitored using headspace gas chromatography (GC) and indirect conductivity (IC). The results from GC in comparison to the standard culture technique revealed the presence of VBNC state when E. coli DH5α and P. putida F1 were in contact with nhexane. L. lactis IO-1 was the most sensitive strain, but the VBNC cells were obvious in the case of soybean and n-decane. Although GC showed better detection sensitivity, the IC technique was more practical and cost-effective. Therefore, GC and IC could be extremely simple and useful methods for monitoring the VBNC state of bacteria, which generally stems from environmental stresses.
    Keywords gas chromatography ; indirect conductivity ; metabolic activity ; viable but nonculturable (vbnc) ; emulsion ; Technology ; T ; Technology (General) ; T1-995 ; Science ; Q ; Science (General) ; Q1-390
    Subject code 621
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher Prince of Songkla University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Improved polarized light microscopic detection of gouty crystals via dissolution with formalin and ethylenediamine tetraacetic acid

    Ruedee Hemstapat / Peeradon Duangiad / Borwornporn Tangketsarawan / Thitiya Phuagpan / Sinthida Chienwiwattanawong / Nuttinee Tangsrianugul / Akio Ojida / Jirarut Wongkongkatep

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    2023  Volume 11

    Abstract: Abstract Conventional polarized light microscopy has been widely used to detect gouty crystals, but its limited sensitivity increases the risk of misidentification. In this study, a number of methods were investigated to improve the sensitivity of ... ...

    Abstract Abstract Conventional polarized light microscopy has been widely used to detect gouty crystals, but its limited sensitivity increases the risk of misidentification. In this study, a number of methods were investigated to improve the sensitivity of polarized light microscopy for the detection of monosodium urate monohydrate (MSUM) and calcium pyrophosphate dihydrate (CPPD) crystals. We found that coating glass slides with poly-L-lysine, a positively charged polymer, improved the attachment of crystals to the glass surface, resulting in clearer crystal images compared to non-coated slides. Additionally, the sensitivity of detection was further enhanced by selective dissolution, in which 40% v/v formalin phosphate buffer was employed to dissolve MSUM crystals but not CPPD while 10% ethylenediamine tetraacetic acid (EDTA) was employed to dissolved CPPD but not MSUM. The other possible interferences were dissolved in both EDTA and formalin solution. These methods were successfully applied to detect gouty crystals in biological milieu, including spiked porcine synovial fluid and inflamed rat subcutaneous air pouch tissues.
    Keywords Medicine ; R ; Science ; Q
    Subject code 500
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Fluorescence Differentiation of ATP-Related Multiple Enzymatic Activities in Synovial Fluid as a Marker of Calcium Pyrophosphate Deposition Disease Using Kyoto Green

    Nattha Yongwattana / Nutsara Mekjinda / Tulyapruek Tawonsawatruk / Itaru Hamachi / Akio Ojida / Jirarut Wongkongkatep

    Molecules, Vol 25, Iss 5, p

    2020  Volume 1116

    Abstract: Calcium pyrophosphate deposition disease (CPPD) is a crystal induced inflammation in joints, and causes severe pain in elderly people. The accumulation of pyrophosphate (PPi) in synovial fluid (SF) results from several enzymatic reactions, especially the ...

    Abstract Calcium pyrophosphate deposition disease (CPPD) is a crystal induced inflammation in joints, and causes severe pain in elderly people. The accumulation of pyrophosphate (PPi) in synovial fluid (SF) results from several enzymatic reactions, especially the highly activated e-NPPs, which catalyze the conversion of ATP to PPi. This study demonstrates the detection of relative catalytic activity of 3 enzymes—ecto-nucleotide pyrophosphatase/phosphodiesterases (e-NPPs), tissue nonspecific alkaline phosphatase (TNAP), and ecto-nucleoside triphosphate diphosphohydrolases (e-NTPDases)—using a single molecular sensor called Kyoto Green. Kyoto Green exhibits excellent performance in sensing the catalytic activity of the commercial representatives of the e-NPPs, TNAP, and e-NTPDases, which are ENPP1, PPase, and apyrase, respectively, in both single-enzyme and multi-enzyme assays. Analysis of SF enzymes in 19 SF samples from human and swine revealed moderate activity of e-NPPs, high activity of e-NTPDases, and low activity of TNAP. Our newly developed method for analysis of multiple enzymatic activities using Kyoto Green in biological SF will assist improvement in accuracy of the CPPD prognosis/diagnosis, which will minimize unnecessary medical procedures.
    Keywords cppd ; pseudogout ; enpp1 ; ppase ; apyrase ; synovial fluid ; fluorescence detection ; molecular sensor ; Organic chemistry ; QD241-441
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: A multicolor and ratiometric fluorescent sensing platform for metal ions based on arene–metal-ion contact

    Anna Kanegae / Yusuke Takata / Ippei Takashima / Shohei Uchinomiya / Ryosuke Kawagoe / Kazuteru Usui / Akira Yamashita / Jirarut Wongkongkatep / Manabu Sugimoto / Akio Ojida

    Communications Chemistry, Vol 4, Iss 1, Pp 1-

    2021  Volume 10

    Abstract: Fluorescent probes can detect metal ions with high sensitivity, but their design typically relies on a limited number of sensing mechanisms. Here the use of arene–metal-ion contact as a sensing mechanism allows ratiometric detection of metal ions across ... ...

    Abstract Fluorescent probes can detect metal ions with high sensitivity, but their design typically relies on a limited number of sensing mechanisms. Here the use of arene–metal-ion contact as a sensing mechanism allows ratiometric detection of metal ions across a broad wavelength range.
    Keywords Chemistry ; QD1-999
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Turning hydrophilic bacteria into biorenewable hydrophobic material with potential antimicrobial activity via interaction with chitosan

    Hanpanich, Orakan / Jirarut Wongkongkatep / Pravit Wongkongkatep / Thunyarat Pongtharangkul

    Bioresource technology. 2017 Apr., v. 230

    2017  

    Abstract: Alteration of a bacteriocin-producing hydrophilic bacterium, Lactococcus lactis IO-1, into a hydrophobic material with potential antimicrobial activity using chitosan was investigated and compared with five other bacterial species with industrial ... ...

    Abstract Alteration of a bacteriocin-producing hydrophilic bacterium, Lactococcus lactis IO-1, into a hydrophobic material with potential antimicrobial activity using chitosan was investigated and compared with five other bacterial species with industrial importance. The negatively charged bacterial cells were neutralized by positively charged chitosan, resulting in a significant increase in the hydrophobicity of the bacterial cell surface. The largest Gram-positive B. megaterium ATCC 14581 showed a moderate response to chitosan while the smaller E. coli DH5α, L. lactis IO-1 and P. putida F1 exhibited a significant response to an increase in chitosan concentration. Because L. lactis IO-1 is a good source for natural peptide lantibiotic that is highly effective against several strains of food spoilage organisms and pathogens, hydrophobic material derived from L. lactis IO-1 and chitosan is a promising novel material with antimicrobial activity for the food and pharmaceutical industries.
    Keywords anti-infective properties ; bacteria ; chitosan ; Escherichia coli ; food spoilage ; hydrophilicity ; hydrophobicity ; Lactococcus lactis ; pathogens ; pharmaceutical industry
    Language English
    Dates of publication 2017-04
    Size p. 97-102.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2017.01.047
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 3258: Show issues

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  7. Article: Influence of pH and ionic strength on the physical and rheological properties and stability of whey protein stabilized o/w emulsions containing xanthan gum

    Sriprablom, Jiratthitikan / Jirarut Wongkongkatep / Manop Suphantharika / Pairoj Luangpituksa / Thunyarat Pongtharangkul

    Journal of food engineering. 2019 Feb., v. 242

    2019  

    Abstract: The influence of pH (3–7) and ionic strength (0–250 mM NaCl) on the physical and rheological properties and stability of soybean oil-in-water emulsions containing whey protein isolate (WPI) as an emulsifier and anionic polysaccharide (xanthan gum, XG) as ...

    Abstract The influence of pH (3–7) and ionic strength (0–250 mM NaCl) on the physical and rheological properties and stability of soybean oil-in-water emulsions containing whey protein isolate (WPI) as an emulsifier and anionic polysaccharide (xanthan gum, XG) as a stabilizer was studied. The influence of ionic strength was found to be dependent on whether the pH was below or above the isoelectric point (pI ≈ 4.6) of the emulsion droplets. At pH below the pI, the ζ-potential of the emulsions increased from negative to positive values with increasing ionic strength. The emulsions exhibited more extensive droplet flocculation, more viscous, and more structured, but less creaming stability at low ionic strength than at high ionic strength. At pH above the pI, the ζ-potential of the emulsions was always negative and its magnitude decreased with increasing ionic strength. The emulsions exhibited a slight increase in droplet flocculation, decrease in viscosity, viscoelasticity, and creaming stability with increasing ionic strength. This study could potentially serve for applications in the food industry to design the emulsion-based food products with improved physical and rheological properties and stability over a wide range of pH and ionic strength conditions.
    Keywords creaming ; droplets ; emulsifiers ; emulsions ; flocculation ; food industry ; foods ; ionic strength ; isoelectric point ; pH ; sodium chloride ; soybeans ; stabilizers ; viscoelasticity ; viscosity ; whey protein isolate ; xanthan gum ; zeta potential
    Language English
    Dates of publication 2019-02
    Size p. 141-152.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 622518-4
    ISSN 0260-8774
    ISSN 0260-8774
    DOI 10.1016/j.jfoodeng.2018.08.031
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 5086: Show issues

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  8. Article ; Online: Fluorescence Detection of Deoxyadenosine in Cordyceps spp. by Indicator Displacement Assay

    Arinta Agnie Dewantari / Nattha Yongwattana / Panwajee Payongsri / Sawinee Seemakhan / Suparerk Borwornpinyo / Akio Ojida / Jirarut Wongkongkatep

    Molecules, Vol 25, Iss 2045, p

    2020  Volume 2045

    Abstract: A rapid, sensitive and reliable indicator displacement assay (IDA) for specific detection of 2′- and 3′-deoxyadenosine (2′-dAde and 3′-dAde), the latter is also known as cordycepin, was established. The formation of inclusion complex between protonated ... ...

    Abstract A rapid, sensitive and reliable indicator displacement assay (IDA) for specific detection of 2′- and 3′-deoxyadenosine (2′-dAde and 3′-dAde), the latter is also known as cordycepin, was established. The formation of inclusion complex between protonated acridine orange (AOH + ) and cucurbit[7]uril (CB7) resulted in the hypochromic shift of fluorescent emission from 530 nm to 512 nm. Addition of cordycepin to the highly fluorescent AOH + /CB7 complex resulted in a unique tripartite AOH + /CB7/dAde complex with diminished fluorescence, and such reduction in emission intensity serves as the basis for our novel sensing system. The detection limits were 11 and 82 μM for 2′- and 3′-deoxyadenosine, respectively. The proposed method also demonstrated high selectivity toward 2′- and 3′-deoxyadenosine, owing to the inability of other deoxynucleosides, nucleosides and nucleotides commonly found in Cordyceps spp. to displace the AOH + from the AOH + /CB7 complex, which was confirmed by isothermal titration calorimetry (ITC), UV-Visible and proton nuclear magnetic resonance ( 1 H-NMR) spectroscopy. Our method was successfully implemented in the analysis of cordycepin in commercially available Ophiocordyceps and Cordyceps supplements, providing a novel and effective tool for quality assessment of these precious fungi with several health benefits.
    Keywords cordycepin ; fungi ; indicator displacement assay ; fluorescence ; detection ; cucurbit uril ; Organic chemistry ; QD241-441
    Subject code 333
    Language English
    Publishing date 2020-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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