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Article ; Online: Distribuição das artérias coronárias e análise histopatológica do coração de Bradypus variegatus

Priscilla Virgínio de Albuquerque / Sandra Maria de Torres / Emanuela Polimeni de Mesquita / Júlio Cézar dos Santos Nascimento / Apolônio Gomes Ribeiro / Joaquim Evêncio Neto / Gilcifran Prestes de Andrade / Adelmar Afonso de Amorim Júnior / Marleyne José Afonso Accioly Lins Amorim

Ciência Animal Brasileira, Vol

2024 Volume 25

Abstract: A preguiça-comum (Bradypus variegatus) tem sido vítima das investidas antrópicas desordenadas, o que tem elevado o número de acidentes e doenças para a espécie. Propôs-se com esse estudo, realizar uma descrição das artérias coronárias, assim como apontar ...

Abstract	A preguiça-comum (Bradypus variegatus) tem sido vítima das investidas antrópicas desordenadas, o que tem elevado o número de acidentes e doenças para a espécie. Propôs-se com esse estudo, realizar uma descrição das artérias coronárias, assim como apontar os achados histopatológicos do coração do bicho-preguiça, a fim de favorecer o diagnóstico por imagem, semiologia e clínica médica das espécies. Foram dissecados 18 exemplares de B. variegatus obtidos após morte natural. Quinze deles, sendo 5 machos e 9 fêmeas adultas e 1 jovem, foram fixados com formaldeído e conservados em solução salina. Em 2 machos e 1 fêmea, foi injetado, a partir da artéria carótida comum esquerda, látex corado em vermelho, para tornar as coronárias mais evidentes. Na fêmea também foi injetado através da veia jugular externa direita, látex corado em azul, para evitar equívoco na identificação arterial. Os animais tiveram o tórax aberto a fim de avaliar e retirar o coração para descrição detalhada das artérias coronárias. Para análise histopatológica, foram confeccionadas lâminas histológicas oriundas de corações coletados de 4 animais (3 adultos e 1 jovem). Com base nas análises arteriais, as preguiças possuem duas coronárias: uma a direita e uma a esquerda, que irrigam suas respectivas faces. O coração é composto por endocárdio, miocárdio e epicárdio, semelhante aos mamíferos em geral. Todavia, observou-se necroses endocárdicas, de fibras musculares cardíacas, além de degenerações mixomatosas de válvulas e trombos no lúmen vascular. Esses eventos necróticos, mais extensos, estão relacionados a infartos, enquanto os mais discretos podem ser associados a situações de estresse vivenciadas pelos espécimes.
Keywords	Agriculture ; S ; Animal culture ; SF1-1100
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Universidade Federal de Goiás
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Tracheal post-vaccinal reaction to different strains of Newcastle disease vírus

Priscilla M.C. Rocha / Maria E.G. Barros / Jéssica T. Bandeira / Juliana F.V. Braga / Renato S.M.M. Morais / Francisco A.L. Souza / Joaquim Evêncio-Neto

Pesquisa Veterinária Brasileira, Vol

2022 Volume 42

Abstract: ABSTRACT: The aim of the present study was to evaluate the post-vaccinal reaction to two lentogenic vaccine strains of Newcatle disease virus (NDV) and a recombinant turkey herpesvirus (rHVT) vaccine expressing the fusion glycoprotein of NDV in broiler ... ...

Abstract	ABSTRACT: The aim of the present study was to evaluate the post-vaccinal reaction to two lentogenic vaccine strains of Newcatle disease virus (NDV) and a recombinant turkey herpesvirus (rHVT) vaccine expressing the fusion glycoprotein of NDV in broiler chickens through histomorphometric and histopathologic analyses of the trachea. The experiment involved 245 chicks housed in randomized blocks with three different enclosures under controlled conditions of temperature, light and ventilation. Each enclosure represented a vaccine strain and was divided into groups according to the administration route. Each block also had its own control group composed of unvaccinated birds. The vaccine strains PHY.LMV.42 (PL42) and La Sota (LS) were selected according to the Intracerebral Pathogenicity Index (ICPI) and the rHVT-NDV Serotype 3 strain (ST3) was selected for representing non-NDV infection. At two, four, seven, 14 and 21 days post vaccination, fragments from the middle third of the trachea were collected and submitted to routine histological processing. For the histomorphometric analysis, the slides were photographed, and the thickness of the tracheal mucosa was measured. Statistical analysis involved two-way ANOVA and Tukey’s post-hoc test with a 5% significance level. For the histopathological evaluation, lesions were described as to the degree of intensity and distribution. At four and 14 days post vaccination with the LS strain administered by the ocular route, the means of thickening of the tracheal mucosa (20.85±7.31μm and 26.97±5.50μm, respectively) were significantly higher (p<0.05) than for all other strains, which was related to the severe histopathological lesions found in this group, characterized by hyperemia, hyperplasia of the mucous glands, moderate deciliation and multifocal lymphohistiocytic inflammatory infiltrate. At 21 days, broiler chickens vaccinated with the ST3 strain showed more discrete lesions and less thickening of the tracheal mucosa (23.23±7.62μm; p<0.05) in comparison with other ...
Keywords	Newcastle disease ; broiler ; post-vaccinal reaction ; trachea ; lentogenic vaccine ; enterotropic vaccine ; vector vaccine ; Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Colégio Brasileiro de Patologia Animal (CBPA)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Pre or perioperative period

Grazielle Anahy de Sousa Aleixo / Maria Cristina de Oliveira Coelho / Miriam Nogueira Teixeira / Lilian Sabrina Silvestre Andrade / Maria Clara Cunha Paranhos de Oliveira / Flávia Corrêa Maia / Ana Luiza Neves Guimarães Bessa / Joaquim Evêncio-Neto / Emanuela Polimeni Mesquita

Medicina Veterinária, Vol 16, Iss

when is the best time to produce platelet-rich plasma in dogs?

2022 Volume 3

Abstract: When evaluating published studies on the production of platelet-rich plasma (PRP), it is possible to observe that there is no consensus on when blood should be collected and processed. Some perform blood collection before surgery, prior to the ... ...

Abstract	When evaluating published studies on the production of platelet-rich plasma (PRP), it is possible to observe that there is no consensus on when blood should be collected and processed. Some perform blood collection before surgery, prior to the administration of drugs, fluids, or any surgical trauma, while others collect blood during the perioperative period. The present objective was to determine the best moment for PRP production based on the platelet count. Blood samples were collected from 20 healthy female dogs at two distinct moments: before surgery, prior to anesthesia and fluid therapy (M0), and later, in the operation room, after administration of the anesthetics and intravenous fluids (M1). To obtain PRP, blood was centrifuged at 1200 rpm for ten minutes, then at 1600 rpm for ten minutes. Results showed that the blood collected at M1 had a 29% decrease in platelet count compared to M0 (highly significant statistical difference). It is recommended that blood collection, which is an essential step for producing platelet-rich plasma, shall be done during the pre-operative period.
Keywords	surgery ; platelet concentrate ; growth factors ; Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	Universidade Federal Rural de Pernambuco
Document type	Article ; Online
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Article ; Online: Análise clínica e histológica de feridas cirúrgicas de cadelas tratadas com um composto fitoterápico de Calendula officinalis, Aloe vera, Symphytum officinale L., Vitis vinifera, D-Pantenol e Benzoato de Denatônio®

Maria Sheila da Silva Ferreira / Grazielle Anahy de Sousa Aleixo / Fábio de Souza Mendonça / Joaquim Evêncio Neto / Mauro Araujo Penaforte Junior / Sabrina Cândido Trajano / Kleyton Domingos Melo / Saulo Romero Felix Gonçalves / Karine Silva Camargo / Gabriela Ratis Galeas

Medicina Veterinária, Vol 16, Iss

2022 Volume 1

Abstract: Esse experimento teve como objetivo avaliar o potencial cicatricial de uma loção fitoterápica formulada com Calendula officinalis – 1,0%, Aloe vera – 1,0%, Symphytum officinale L. – 1,0%, Vitis vinifera – 1,0%, D-Pantenol – 3,0% e Benzoato de Denatônio – ...

Abstract	Esse experimento teve como objetivo avaliar o potencial cicatricial de uma loção fitoterápica formulada com Calendula officinalis – 1,0%, Aloe vera – 1,0%, Symphytum officinale L. – 1,0%, Vitis vinifera – 1,0%, D-Pantenol – 3,0% e Benzoato de Denatônio – 0,02%, (PSK Repair System®) que foi aplicada topicamente sobre feridas cirúrgicas de cadelas. O grupo experimental foi composto por 30 cadelas que foram submetidas à ovariohisterectomia (OH), sendo realizada uma incisão de cinco centímetros na linha abdominal ventral para acesso cirúrgico, e a ferida foi dividida em duas partes iguais de 2,5 cm. No pós-operatório foi aplicada diariamente, durante sete dias, sobre a parte cranial a loção fitoterápica (Grupo Tratado - GT), enquanto que na parte caudal foi aplicada uma loção à base de vaselina (Grupo Controle - GC). Com sete (D7), 14 (D14) e 21 (D21) dias foram escolhidas aleatoriamente cinco pacientes de cada grupo para realização de análise macroscópica da ferida cirúrgica e exame histopatológico. Clinicamente se observou que no GT houve melhora no processo de reparação tecidual, fato esse que foi reforçado pelo exame histopatológico no qual foi possível observar que a presença de infiltrados mononucleares foi menor, enquanto que a presença de fibroblastos e neovascularização se apresentaram de forma mais efetiva que no GC. Diante dos achados, conclui-se que a loção fitoterápica agiu sobre a ferida cirúrgica ajudando no reparo tecidual, suscitando subsídios científicos que fundamentem seu uso como cicatrizante na rotina cirúrgica veterinária.
Keywords	cão ; cicatrização ; fitoterapia ; pele ; Veterinary medicine ; SF600-1100
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	Universidade Federal Rural de Pernambuco
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Análise morfométrica da parede intestinal e dinâmica de mucinas secretadas no jejuno de frangos suplementados com probiótico Bacillus subtilis cepa C3102

Priscilla M.C. Rocha / Maria E.G. Barros / Joaquim Evêncio-Neto

Pesquisa Veterinária Brasileira, Vol 36, Iss 4, Pp 312-

2016 Volume 316

Abstract: Resumo O objetivo deste estudo foi analisar a morfometria da parede intestinal e a dinâmica de mucinas secretadas no jejuno de frangos de corte suplementadas com probiótico Bacillus subtilis cepa C3102. Foram utilizados 720 pintinhos com um dia de vida, ... ...

Abstract	Resumo O objetivo deste estudo foi analisar a morfometria da parede intestinal e a dinâmica de mucinas secretadas no jejuno de frangos de corte suplementadas com probiótico Bacillus subtilis cepa C3102. Foram utilizados 720 pintinhos com um dia de vida, divididos em dois grupos: GA (Grupo controle) - aves alimentadas com ração sem suplementação de Bacillus subtilis cepa C3102 (1º ao 35º dia) e GB (Grupo tratado) - aves alimentadas com ração com suplementação de Bacillus subtilis cepa C3102 (1º ao 35º dia). Aos 7 e 35 dias 2cm do jejuno foram coletados e submetidos à rotina de processamento histológico. Cortes transversais de 1μm foram corados pela técnica do Alcian Blue (AB) para detecção de sialomucinas (pH 0,5) e sulfomucinas (pH 2,5), como também para contagem de células caliciformes presentes. A análise histoquímica revelou uma maior quantidade de sialomucinas presentes no jejuno dos animais que foram suplementados com probiótico Bacillus subtilis cepa C3102 (GB), quando comparados com os animais do grupo controle (GA). O número de células caliciformes presentes no grupo tratado (GB) foi superior ao grupo controle (GA).
Keywords	Frangos de corte ; Bacillus subtilis ; células caliciformes ; mucinas ; Veterinary medicine ; SF600-1100
Language	English
Publishing date	2016-04-01T00:00:00Z
Publisher	Colégio Brasileiro de Patologia Animal (CBPA)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Evaluation of platelet-rich plasma gel as an angiogenesis-inducing agent in canineadvancement skin flaps

Grazielle A.S. Aleixo / Maria C.O.C. Coelho / Telga L.A. Almeida / Márcia F. Pereira / Miriam N. Teixeira / Lilian S.S. Andrade / Ana Luiza N.G. Bessa / Joaquim Evêncio-Neto

Pesquisa Veterinária Brasileira, Vol 40, Iss 6, Pp 474-

2020 Volume 478

Abstract: ABSTRACT: This work aimed to evaluate the effect of platelet-rich plasma (PRP) on advancement skin flaps in dogs regarding improvement of vascularization, with focus on increasing its viable area, since there are reports that it is a potential ... ...

Abstract	ABSTRACT: This work aimed to evaluate the effect of platelet-rich plasma (PRP) on advancement skin flaps in dogs regarding improvement of vascularization, with focus on increasing its viable area, since there are reports that it is a potential angiogenesis stimulator. The experimental group was composed of eight adult bitches, in which two advancement skin flaps were made in the ventral abdominal region. No product was applied in the control flap (CF), while PRP was used in the contralateral flap, called treated flap (TF). The areas were clinically evaluated every two days until the 7th postoperative day regarding skin color and presence of necrosis. At 10 days, both flaps were removed and submitted to histological examination and blood vessel morphometry. The vessels counted in each group were statistically analyzed by the F-test at 1% probability. Results showed no significant difference in macroscopic changes in the wound, or CF and TF vascularization, thus suggesting that PRP gel did not improve advancement skin flap angiogenesis in bitches under the experimental conditions in which this research was developed.
Keywords	Platelet-rich plasma gel ; angiogenesis ; canine ; skin flap ; platelet gel ; growth factors ; dogs ; Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2020-08-01T00:00:00Z
Publisher	Colégio Brasileiro de Patologia Animal (CBPA)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Morphological description of ovary and uterus of the nurse shark (Ginglymostoma cirratum) caught off at the Fortaleza coast, Northeast Brazil

Mariana G. Rêgo / Maria Lucia G. Araujo / Maria Edna G. Barros / Lorena D’Andrade Aires / Paulo G.V. Oliveira / Fábio H.V. Hazin / John L. Fitzpatrick / Joaquim Evêncio-Neto

Pesquisa Veterinária Brasileira, Vol 39, Iss 12, Pp 997-

2020 Volume 1004

Abstract: ABSTRACT: The nurse shark, Ginglymostoma cirratum (Bonnaterre, 1778) is one of the most studied species of elasmobranchs. However, the knowledge of their reproductive biology is still relatively rare, particularly in the western South Atlantic. This ... ...

Abstract	ABSTRACT: The nurse shark, Ginglymostoma cirratum (Bonnaterre, 1778) is one of the most studied species of elasmobranchs. However, the knowledge of their reproductive biology is still relatively rare, particularly in the western South Atlantic. This study aimed to describe the morphology of the uterus and the ovary of G. cirratum, based on specimens caught off at the Fortaleza/CE coast, northeast Brazil. Samples were collected from September 2012 to June 2013, from regular landings of artisanal fishing, which commercialize this species freely. A total of ten females were collected. The methodologies followed for analyzing the ovaries and uterus of those females included both macroscopic and histological analysis. G. cirratum has internal type ovary morphology, with invaginations of connective tissue, which defines compartments and separate oocyte groups in ovigerous lots. The epithelium lining the ovary changes from simple columnar ciliated in the area without ovigerous lots, which turns into a simple cubic epithelium in the coating portion of the epigonal organ where ovarian tissue is absent. The uterine mucosa has secretory cells denoted by Alcian Blue staining, indicating the production of mucopolysaccharides, even in immature individuals. This lecithotrophic shark has a uterine vascularized mucosa that is one characteristic of viviparous elasmobranch species.
Keywords	Morphology ; ovary ; uterus ; nurse shark ; Ginglymostoma cirratum ; Fortaleza ; Brazil ; female ; reproduction ; histology ; sharks ; Veterinary medicine ; SF600-1100
Subject code	590
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Colégio Brasileiro de Patologia Animal (CBPA)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Equine coital exanthema caused by equid alphaherpesvirus 3

Givaldo B. Silva Filho / Hisadora A.S.C Bom / Silvio M.C. Fonseca / Érica A. Costa / Beatriz S.A.S. Santos / Renato L. Santos / Francisco A.L. Souza / Joaquim Evêncio Neto / Fábio S. Mendonça

Pesquisa Veterinária Brasileira, Vol

a report of an outbreak in northeastern Brazil

2021 Volume 41

Abstract: ABSTRACT: Equine coital exanthema is a venereal infectious disease poorly reported in horses in Brazil and was never described in the northeastern region of the country. This work aims to describe the clinical and pathological aspects of an outbreak of ... ...

Abstract	ABSTRACT: Equine coital exanthema is a venereal infectious disease poorly reported in horses in Brazil and was never described in the northeastern region of the country. This work aims to describe the clinical and pathological aspects of an outbreak of equine coital exanthema caused by equid alphaherpesvirus 3, occurred in a herd of horses at the semiarid region of the State of Rio Grande do Norte. Main clinical signs consisted of anorexia, hiporexia, fibrinous or purulent secretion in the penis mucosa and vagina. Two mares presented mild to minimal lesions that consisted of scars in the mucosa of the vagina and in the perivulvar region. In a stallion the disease consisted of severe, multifocal, umbilicated-exanthematous ulcers of approximately 1cm in diameter on the penis mucosa. Other areas where ulcers and crusts were focally observed included the skin of the scrotum and on the lips and mucocutaneous junctions of the oral cavity. Histologically, the main lesion consisted of multifocal severe ulcerative and fibrinous necrotizing balanoposthitis and mild multifocal necrotizing, lymphocytic dermatitis in the lips and scrotum. The equide alphaherpesvirus 3 DNA was amplified in blood samples and penis mucosa using the PCR technique. This is the first report of molecular diagnosis of equine coital exanthema affecting horses in northeastern Brazil. Further studies should be carried out in order to investigate the epidemiology and the importance of this herpetic disease in the country.
Keywords	Equine coital exanthema ; equid alphaherpesvirus 3 ; Brazil ; horses ; balanopostitis ; vaginitis ; Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2021-07-01T00:00:00Z
Publisher	Colégio Brasileiro de Patologia Animal (CBPA)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Phytochemical screening and antimicrobial activity testing of crude hydroalcoholic extract from leaves of Sphagneticola trilobata (Asteraceae)

Ana Greice Borba Leite / Eulina Tereza Nery Farias / Ana Paula de Oliveira / Ruan Emmanuell Franco de Abreu / Mateus Matiuzzi da Costa / Jackson Roberto Guedes da Silva Almeida / Lígia Reis de Moura Estevão / Joaquim Evêncio-Neto

Ciência Rural, Vol 49, Iss

2019 Volume 4

Abstract: ABSTRACT: This study aimed to perform phytochemical analysis and to test the antimicrobial activity of the crude hydroalcoholic extract obtained from the leaves of Sphagneticola trilobata. Classes of secondary metabolites present in the extract were ... ...

Abstract	ABSTRACT: This study aimed to perform phytochemical analysis and to test the antimicrobial activity of the crude hydroalcoholic extract obtained from the leaves of Sphagneticola trilobata. Classes of secondary metabolites present in the extract were identified through phytochemical screening using analytical thin-layer chromatography. Antimicrobial activity was evaluated by testing cultures of Staphylococcus aureus, S. epidermidis, Staphylococcus spp., Escherichia coli, Serratia marcescens, Enterococcus faecalis, Pseudomonas aeruginosa, Salmonella Typhimurium, and Klebsiella pneumoniae isolated from human skin and those of Staphylococcus spp. isolated from dog skin using the broth microdilution method. In the phytochemical screening, classes of anthracenic derivatives and mono-, sesqui-, and diterpenes were identified. Colorimetric analysis showed total phenol and total flavonoid contents of 21.7 ± 0.009 mg of gallic acid equivalents per gram of sample and 0.23 ± 0.005 mg of catechin equivalents per gram of sample, respectively. Microbiological analysis revealed that the hydroalcoholic extract of S. trilobata exhibited antimicrobial activity against cultures of Staphylococcus spp., E. coli, S. marcescens, and E. faecalis isolated from human skin and those of Staphylococcus spp. isolated from dog skin. Thus, crude hydroalcoholic extract of leaves of S. trilobata contained flavonoids and terpenoids as secondary metabolites, which contributed to its antimicrobial activity against skin bacteria isolated from different sources.
Keywords	antibacteriano ; estudo fitoquímico ; compostos fenólicos ; Agriculture ; S ; Agriculture (General) ; S1-972
Subject code	571
Language	English
Publishing date	2019-04-01T00:00:00Z
Publisher	Universidade Federal de Santa Maria
Document type	Article ; Online
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Article ; Online: HISTOCHEMICAL ANALYSIS OF THE TONGUE IN NEOTROPICAL FRUGIVOROUS BATS (MAMMALIA – CHIROPTERA) WITH REPORT OF EPITHELIAL PIGMENTATION

Eveline de Cássia Batista de Almeida Alves / Maria Juliana Gomes Arandas / Nivaldo Bernardo de Lima-Junior / Joaquim Evêncio Neto / Katharine Raquel Pereira dos Santos

Ciência Animal Brasileira, Vol 18, Iss

2017

Abstract: The tongue is regarded as a key organ for bats, since the specialized characters are used for taxonomic and systematic purposes, cladistic studies, and elucidation of patterns of eating habits. This study aimed to characterize morphologically the tongue ... ...

Abstract	The tongue is regarded as a key organ for bats, since the specialized characters are used for taxonomic and systematic purposes, cladistic studies, and elucidation of patterns of eating habits. This study aimed to characterize morphologically the tongue in the species Artibeus lituratus, Artibeus planirostris, Artibeus obscurus, and Dermanura cinerea, and to report the first evidence of melanin in the lingual epithelium of bats. We used 20 specimens collected in the municipalities of Vitória de Santo Antão and Sirinhaém, in Pernambuco State, Brazil. The tongues were extracted and submitted to routine histological techniques, stained with hematoxylin and eosin (HE) and Fontana-Masson. In the four species, the keratinized stratified pavement epithelium was observed, but with the presence of melanin granules on the basal layer only in the species A. lituratus, A. planirostris, and A. obscurus, evidenced through the Fontana-Masson staining technique. The melanin found in the tongue of these species is characterized as the first record for the order Chiroptera and it may be related to oral asepsis, and/or inhibition of toxic substances observed in plants. Therefore, there is a need for further studies, aiming to improve our knowledge of the function of pigment in this organ. Keywords: Artibeus; Dermanura; melanin; bats; lingual morphology.
Keywords	Artibeus ; Dermanura ; melanina ; morcegos ; morfologia lingual ; Agriculture ; S ; Animal culture ; SF1-1100
Subject code	590
Language	English
Publishing date	2017-03-01T00:00:00Z
Publisher	Universidade Federal de Goiás
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