Article ; Online: Accurate prostate cancer detection based on enrichment and characterization of prostate cancer specific circulating tumor cells.
2023 Volume 12, Issue 8, Page(s) 9116–9127
Abstract: Background: The low specificity of serum PSA resulting in the inability to effectively differentiate prostate cancer from benign prostate conditions is a persistent clinical challenge. The low sensitivity of serum PSA results in false negatives and can ... ...
| Abstract | Background: The low specificity of serum PSA resulting in the inability to effectively differentiate prostate cancer from benign prostate conditions is a persistent clinical challenge. The low sensitivity of serum PSA results in false negatives and can miss high-grade prostate cancers. We describe a non-invasive test for detection of prostate cancer based on functional enrichment of prostate adenocarcinoma associated circulating tumor cells (PrAD-CTCs) from blood samples followed by their identification by immunostaining for pan-cytokeratins (PanCK), prostate specific membrane antigen (PSMA), alpha methyl-acyl coenzyme-A racemase (AMACR), epithelial cell adhesion molecule (EpCAM), and common leucocyte antigen (CD45). Methods: Analytical validation studies were performed to establish the performance characteristics of the test using VCaP prostate cancer cells spiked into healthy donor blood (HDB). The clinical performance characteristics of the test were evaluated in a case-control study with 160 known prostate cancer cases and 800 healthy males, followed by a prospective clinical study of 210 suspected cases of prostate cancer. Results: Analytical validation established analyte stability as well as acceptable performance characteristics. The test showed 100% specificity and 100% sensitivity to differentiate prostate cancer cases from healthy individuals in the case control study and 91.2% sensitivity and 100% specificity to differentiate prostate cancers from benign prostate conditions in the prospective clinical study. Conclusions: The test accurately detects PrAD-CTCs with high sensitivity and specificity irrespective of stage, serum PSA or Gleason score, which translates into low risks of false negatives or overdiagnosis. The high accuracy of the test could offer advantages over PSA based prostate cancer detection. |
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| MeSH term(s) | Male ; Humans ; Prostate-Specific Antigen ; Prostate/pathology ; Case-Control Studies ; Neoplastic Cells, Circulating ; Prospective Studies ; Prostatic Neoplasms/pathology ; Biomarkers, Tumor |
| Chemical Substances | Prostate-Specific Antigen (EC 3.4.21.77) ; Biomarkers, Tumor |
| Language | English |
| Publishing date | 2023-01-30 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2659751-2 |
| ISSN | 2045-7634 ; 2045-7634 |
| ISSN (online) | 2045-7634 |
| ISSN | 2045-7634 |
| DOI | 10.1002/cam4.5649 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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