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  1. Article ; Online: A technique for a single-stage off-pump repair of Kommerell diverticulum with antegrade branch vessel reconstruction.

    Bedeir, Kareem / Johnson, William E

    JTCVS techniques

    2022  Volume 17, Page(s) 14–17

    Language English
    Publishing date 2022-11-03
    Publishing country United States
    Document type Journal Article
    ISSN 2666-2507
    ISSN (online) 2666-2507
    DOI 10.1016/j.xjtc.2022.10.015
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  2. Article ; Online: Concomitant carotid-carotid-axillary bypass with zone 0 branched endograft and transcatheter aortic valve-in-valve replacement via retroperitoneal access.

    Bedeir, Kareem / McRee, Chad / Johnson, William E

    JTCVS techniques

    2023  Volume 21, Page(s) 26–31

    Language English
    Publishing date 2023-08-11
    Publishing country United States
    Document type Journal Article
    ISSN 2666-2507
    ISSN (online) 2666-2507
    DOI 10.1016/j.xjtc.2023.07.025
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Sirtuin 2 inhibition modulates chromatin landscapes genome-wide to induce senescence in ATRX-deficient malignant glioma.

    Malgulwar, Prit Benny / Danussi, Carla / Dharmaiah, Sharvari / Johnson, William E / Rao, Arvind / Huse, Jason T

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Inactivating mutations in : One sentence summary: Our study demonstrates that SIRT2 inhibition promotes senescence in ATRX-deficient glioma model systems through global epigenomic remodeling, impacting key downstream transcriptional profiles. ...

    Abstract Inactivating mutations in
    One sentence summary: Our study demonstrates that SIRT2 inhibition promotes senescence in ATRX-deficient glioma model systems through global epigenomic remodeling, impacting key downstream transcriptional profiles.
    Language English
    Publishing date 2023-01-10
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.01.09.523324
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: The Comparative Effects of Mesenchymal Stem Cell Transplantation Therapy for Spinal Cord Injury in Humans and Animal Models: A Systematic Review and Meta-Analysis.

    Johnson, Louis D V / Pickard, Mark R / Johnson, William E B

    Biology

    2021  Volume 10, Issue 3

    Abstract: Animal models have been used in preclinical research to examine potential new treatments for spinal cord injury (SCI), including mesenchymal stem cell (MSC) transplantation. MSC transplants have been studied in early human trials. Whether the animal ... ...

    Abstract Animal models have been used in preclinical research to examine potential new treatments for spinal cord injury (SCI), including mesenchymal stem cell (MSC) transplantation. MSC transplants have been studied in early human trials. Whether the animal models represent the human studies is unclear. This systematic review and meta-analysis has examined the effects of MSC transplants in human and animal studies. Following searches of PubMed, Clinical Trials and the Cochrane Library, published papers were screened, and data were extracted and analysed. MSC transplantation was associated with significantly improved motor and sensory function in humans, and significantly increased locomotor function in animals. However, there are discrepancies between the studies of human participants and animal models, including timing of MSC transplant post-injury and source of MSCs. Additionally, difficulty in the comparison of functional outcome measures across species limits the predictive nature of the animal research. These findings have been summarised, and recommendations for further research are discussed to better enable the translation of animal models to MSC-based human clinical therapy.
    Language English
    Publishing date 2021-03-16
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology10030230
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: ST2-Conditioned Medium Fosters Dorsal Horn Cell Excitability and Synaptic Transmission in Cultured Mouse Spinal Cord.

    Juárez, Esri H / Wood, Chelsea R / Davies, Rebecca / Kehoe, Oksana / Johnson, William E B / Merighi, Adalberto / Ferrini, Francesco

    Stem cell reviews and reports

    2023  Volume 19, Issue 8, Page(s) 2918–2928

    Abstract: Conditioned medium obtained from bone marrow-derived stem cells has been proposed as a novel cell-free therapy in spinal cord injury and neuropathic pain, yet the direct effect on spinal neuron function has never been investigated. Here, we adopted ... ...

    Abstract Conditioned medium obtained from bone marrow-derived stem cells has been proposed as a novel cell-free therapy in spinal cord injury and neuropathic pain, yet the direct effect on spinal neuron function has never been investigated. Here, we adopted spinal cord organotypic cultures (SCOCs) as an experimental model to probe the effect of ST2 murine mesenchymal stem cells-conditioned medium (ST2-CM) on dorsal horn (DH) neuron functional properties. Three days of SCOC exposure to ST2-CM increased neuronal activity measured by Fos expression, as well as spontaneous or induced firing. We showed that the increase in neuronal excitability was associated with changes in both intrinsic membrane properties and an enhanced excitatory drive. The increased excitability at the single-cell level was substantiated at the network level by detecting synchronous bursts of calcium waves across DH neurons. Altogether, SCOCs represent a viable tool to probe mesenchymal cells' effect on intact neuronal networks. Our findings indicate that ST2-CM enhances neuronal activity and synaptic wiring in the spinal dorsal horn. Our data also support the trophic role of mesenchymal cells CM in maintaining network activity in spinal circuits.
    MeSH term(s) Animals ; Mice ; Culture Media, Conditioned/pharmacology ; Culture Media, Conditioned/metabolism ; Posterior Horn Cells/metabolism ; Spinal Cord Dorsal Horn/metabolism ; Synaptic Transmission
    Chemical Substances Culture Media, Conditioned
    Language English
    Publishing date 2023-09-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2495577-2
    ISSN 2629-3277 ; 1558-6804 ; 1550-8943
    ISSN (online) 2629-3277 ; 1558-6804
    ISSN 1550-8943
    DOI 10.1007/s12015-023-10618-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: The Comparative Effects of Mesenchymal Stem Cell Transplantation Therapy for Spinal Cord Injury in Humans and Animal Models: A Systematic Review and Meta-Analysis

    Johnson, Louis D. V / Pickard, Mark R / Johnson, William E. B

    Biology. 2021 Mar. 16, v. 10, no. 3

    2021  

    Abstract: Animal models have been used in preclinical research to examine potential new treatments for spinal cord injury (SCI), including mesenchymal stem cell (MSC) transplantation. MSC transplants have been studied in early human trials. Whether the animal ... ...

    Abstract Animal models have been used in preclinical research to examine potential new treatments for spinal cord injury (SCI), including mesenchymal stem cell (MSC) transplantation. MSC transplants have been studied in early human trials. Whether the animal models represent the human studies is unclear. This systematic review and meta-analysis has examined the effects of MSC transplants in human and animal studies. Following searches of PubMed, Clinical Trials and the Cochrane Library, published papers were screened, and data were extracted and analysed. MSC transplantation was associated with significantly improved motor and sensory function in humans, and significantly increased locomotor function in animals. However, there are discrepancies between the studies of human participants and animal models, including timing of MSC transplant post-injury and source of MSCs. Additionally, difficulty in the comparison of functional outcome measures across species limits the predictive nature of the animal research. These findings have been summarised, and recommendations for further research are discussed to better enable the translation of animal models to MSC-based human clinical therapy.
    Keywords animal injuries ; animal research ; cell transplantation ; humans ; mesenchymal stromal cells ; meta-analysis ; systematic review
    Language English
    Dates of publication 2021-0316
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology10030230
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Mesenchymal stem cell conditioned medium increases glial reactivity and decreases neuronal survival in spinal cord slice cultures.

    Wood, Chelsea R / Juárez, Esri H / Ferrini, Francesco / Myint, Peter / Innes, John / Lossi, Laura / Merighi, Adalberto / Johnson, William E B

    Biochemistry and biophysics reports

    2021  Volume 26, Page(s) 100976

    Abstract: ... Ex ... ...

    Abstract Ex vivo
    Language English
    Publishing date 2021-03-03
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2831046-9
    ISSN 2405-5808 ; 2405-5808
    ISSN (online) 2405-5808
    ISSN 2405-5808
    DOI 10.1016/j.bbrep.2021.100976
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  8. Article ; Online: An In Vitro Comparison of the Neurotrophic and Angiogenic Activity of Human and Canine Adipose-Derived Mesenchymal Stem Cells (MSCs): Translating MSC-Based Therapies for Spinal Cord Injury.

    Delfi, Ibtesam R T Al / Wood, Chelsea R / Johnson, Louis D V / Snow, Martyn D / Innes, John F / Myint, Peter / Johnson, William E B

    Biomolecules

    2020  Volume 10, Issue 9

    Abstract: The majority of research into the effects of mesenchymal stem cell (MSC) transplants on spinal cord injury (SCI) is performed in rodent models, which may help inform on mechanisms of action, but does not represent the scale and wound heterogeneity seen ... ...

    Abstract The majority of research into the effects of mesenchymal stem cell (MSC) transplants on spinal cord injury (SCI) is performed in rodent models, which may help inform on mechanisms of action, but does not represent the scale and wound heterogeneity seen in human SCI. In contrast, SCI in dogs occurs naturally, is more akin to human SCI, and can be used to help address important aspects of the development of human MSC-based therapies. To enable translation to the clinic and comparison across species, we have examined the paracrine, regenerative capacity of human and canine adipose-derived MSCs in vitro. MSCs were initially phenotyped according to tissue culture plastic adherence, cluster of differentiation (CD) immunoprofiling and tri-lineage differentiation potential. Conditioned medium (CM) from MSC cultures was then assessed for its neurotrophic and angiogenic activity using established cell-based assays. MSC CM significantly increased neuronal cell proliferation, neurite outgrowth, and βIII tubulin immunopositivity. In addition, MSC CM significantly increased endothelial cell migration, cell proliferation and the formation of tubule-like structures in Matrigel assays. There were no marked or significant differences in the capacity of human or canine MSC CM to stimulate neuronal cell or endothelial cell activity. Hence, this study supports the use of MSC transplants for canine SCI; furthermore, it increases understanding of how this may subsequently provide useful information and translate to MSC transplants for human SCI.
    MeSH term(s) Animals ; Cell Line ; Cell Movement/physiology ; Cell Proliferation/physiology ; Collagen ; Culture Media, Conditioned ; Dogs ; Drug Combinations ; Endothelial Cells/physiology ; Humans ; In Vitro Techniques ; Laminin ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/physiology ; Neovascularization, Physiologic ; Neuronal Outgrowth/physiology ; Neurons/physiology ; Paracrine Communication ; Proteoglycans ; Spinal Cord Injuries/therapy ; Tubulin/metabolism
    Chemical Substances Culture Media, Conditioned ; Drug Combinations ; Laminin ; Proteoglycans ; Tubulin ; matrigel (119978-18-6) ; Collagen (9007-34-5)
    Language English
    Publishing date 2020-09-09
    Publishing country Switzerland
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom10091301
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Exposing mesenchymal stem cells to chondroitin sulphated proteoglycans reduces their angiogenic and neuro-adhesive paracrine activity.

    Wood, Chelsea R / Al Delfi, Ibtesam R T / Innes, John F / Myint, Peter / Johnson, William E B

    Biochimie

    2018  Volume 155, Page(s) 26–36

    Abstract: The multifactorial complexity of spinal cord injuries includes the formation of a glial scar, of which chondroitin sulphated proteoglycans (CSPG) are an integral component. Previous studies have shown CSPG to have inhibitory effects on endothelial and ... ...

    Abstract The multifactorial complexity of spinal cord injuries includes the formation of a glial scar, of which chondroitin sulphated proteoglycans (CSPG) are an integral component. Previous studies have shown CSPG to have inhibitory effects on endothelial and neuronal cell growth, highlighting the difficulty of spinal cord regeneration. Mesenchymal stem/stromal cells (MSC) are widely used as a cell therapy, and there is mounting evidence for their angiogenic and neurotrophic paracrine properties. However, in vivo studies have observed poor engraftment and survival of MSC when injected into SCI. Currently, it is not known whether increasing CSPG concentrations seen after SCI may affect MSC; therefore we have investigated the effects of CSPG exposure to MSC in vitro. CSPG-mediated inhibition of MSC adhesion was observed when MSC were cultured on substrates of increasing CSPG concentration, however MSC viability was not affected even up to five days of culture. Culture conditioned medium harvested from these cultures (primed MSC CM) was used as both culture substrata and soluble medium for EA.hy926 endothelial cells and SH-SY5Y neuronal cells. MSC CM was angiogenic, promoting endothelial cell adhesion, proliferation and tubule formation. However, exposing MSC to CSPG reduced the effects of CSPG-primed MSC CM on endothelial cell adhesion and proliferation, but did not reduce MSC-induced endothelial tubule formation. Primed MSC CM also promoted neuronal cell adhesion, which was reduced following exposure to CSPG. There were no marked differences in neurite outgrowth in MSC CM from CSPG primed MSC cultures versus control conditions, although non-primed MSC CM from the same donors was found to significantly enhance neurite outgrowth. Taken together, these studies demonstrate that MSC are resilient to CSPG exposure, but that there is a marked effect of CSPG on their paracrine regenerative activity. The findings increase our understanding of how the wound microenvironment after SCI can mitigate the beneficial effects of MSC transplantation.
    MeSH term(s) Cell Adhesion ; Cell Line, Tumor ; Chondroitin Sulfates/metabolism ; Humans ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism ; Neovascularization, Physiologic ; Neurites/metabolism ; Paracrine Communication ; Proteoglycans/metabolism
    Chemical Substances Proteoglycans ; Chondroitin Sulfates (9007-28-7)
    Language English
    Publishing date 2018-04-19
    Publishing country France
    Document type Journal Article
    ZDB-ID 120345-9
    ISSN 1638-6183 ; 0300-9084
    ISSN (online) 1638-6183
    ISSN 0300-9084
    DOI 10.1016/j.biochi.2018.04.011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: The influence of pH and fluid dynamics on the antibacterial efficacy of 45S5 Bioglass.

    Begum, Saima / Johnson, William E / Worthington, Tony / Martin, Richard A

    Biomedical materials (Bristol, England)

    2016  Volume 11, Issue 1, Page(s) 15006

    Abstract: In recent years, there has been considerable interest in the potential antibacterial properties that bioactive glasses may possess. However, there have been several conflicting reports on the antibacterial efficacy of 45S5 Bioglass(®). Various mechanisms ...

    Abstract In recent years, there has been considerable interest in the potential antibacterial properties that bioactive glasses may possess. However, there have been several conflicting reports on the antibacterial efficacy of 45S5 Bioglass(®). Various mechanisms regarding its mode of action have been proposed, such as changes in the environmental pH, increased osmotic pressure, and 'needle-like' sharp glass debris which could potentially damage prokaryotic cell walls and thus inactivate bacteria. In this current study, a systematic investigation was undertaken on the antibacterial efficacy of 45S5 Bioglass(®) on Escherichia coli NCTC 10538 and Staphylococcus aureus ATCO 6538 under a range of clinically relevant scenarios including varying Bioglass(®) concentration, direct and indirect contact between Bioglass(®) and microorganisms, static and shaking incubation conditions, elevated and neutralised pH environments. The results demonstrated that, under elevated pH conditions, Bioglass(®) particles have no antibacterial effect on S. aureus while a concentration dependent antibacterial effect against E. coli was observed. However, the antibacterial activity ceased when the pH of the media was neutralised. The results of this current study, therefore, suggest that the mechanism of antibacterial activity of Bioglass(®) is associated with changes in the environmental pH; an environment that is less likely to occur in vivo due to buffering of the system.
    MeSH term(s) Anti-Bacterial Agents/administration & dosage ; Anti-Bacterial Agents/chemistry ; Bacterial Adhesion/drug effects ; Bacterial Adhesion/physiology ; Bacterial Physiological Phenomena/drug effects ; Bone Substitutes/chemistry ; Bone Substitutes/pharmacology ; Cell Survival ; Ceramics/chemistry ; Ceramics/pharmacology ; Glass/chemistry ; Hydrogen-Ion Concentration ; Materials Testing ; Rheology/methods
    Chemical Substances Anti-Bacterial Agents ; Bone Substitutes ; bioactive glass 45S5
    Language English
    Publishing date 2016-02-02
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2265222-X
    ISSN 1748-605X ; 1748-6041
    ISSN (online) 1748-605X
    ISSN 1748-6041
    DOI 10.1088/1748-6041/11/1/015006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6626: Show issues Location:
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