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  1. Article ; Online: Recurrence of Human Babesiosis Caused by Reinfection

    Jonathan Ho / Erin Carey / Dennis E. Carey / Peter J. Krause

    Emerging Infectious Diseases, Vol 27, Iss 10, Pp 2658-

    2021  Volume 2661

    Abstract: Babesiosis developed in a 62-year-old immunocompetent physician, who had an uneventful recovery after receiving atovaquone and azithromycin. Three years later, babesiosis developed again, and he was again successfully given treatment. Clinical and ... ...

    Abstract Babesiosis developed in a 62-year-old immunocompetent physician, who had an uneventful recovery after receiving atovaquone and azithromycin. Three years later, babesiosis developed again, and he was again successfully given treatment. Clinical and laboratory evidence were highly supportive of Babesia reinfection. Healthcare professionals should be aware that reinfection might occur in babesiosis.
    Keywords babesiosis ; human babesiosis ; reinfection ; recurrence ; Babesia microti ; parasites ; Medicine ; R ; Infectious and parasitic diseases ; RC109-216
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Centers for Disease Control and Prevention
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Warren Alpert Medical School of Brown University Annual Research Forum

    Jonathan Ho / Mica Kane / Mohammed Haji / Nirav Haribhakti / Thomas Fritz Siegert / Tiffany Ho / Matthew Murphy

    Brown Journal of Hospital Medicine, Vol 2, Iss

    Resident Project Winners

    2023  Volume 3

    Keywords Medicine ; R
    Language English
    Publishing date 2023-07-01T00:00:00Z
    Publisher Department of Medicine, Warren Alpert Medical School at Brown University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: The Formation of Stromules In Vitro from Chloroplasts Isolated from Nicotiana benthamiana.

    Jonathan Ho / Steven M Theg

    PLoS ONE, Vol 11, Iss 2, p e

    2016  Volume 0146489

    Abstract: Stromules are stroma-containing tubules that have been observed to emanate from the main plastidic body in vivo. These structures have been shown to require cytoskeletal components for movement. Though numerous studies have shown a close association with ...

    Abstract Stromules are stroma-containing tubules that have been observed to emanate from the main plastidic body in vivo. These structures have been shown to require cytoskeletal components for movement. Though numerous studies have shown a close association with the endoplasmic reticulum, nucleus, mitochondria, and other plastids, the mechanism of formation and their overall function remain unknown. A limiting factor in studying these structures has been the lack of a reconstituted system for in vitro stromule formation. In this study, stromule formation was induced in vitro by adding a plant extract fraction that is greater than 100 kDa to a population of isolated chloroplasts. Kinetic measurements show that stromule formation occurs within ~10 seconds after the addition of the plant extract fraction. Heat inactivation and apyrase treatment reveal that the stromule stimulating compound found in the extract fraction is a protein or protein complex 100 kDa or greater. The formation of the stromules in vitro with isolated chloroplasts and a concentrated fraction of cell extract opens an avenue for the biochemical dissection of this process that has heretofore been studied only in vivo.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2016-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Odor blocking of stress hormone responses

    Eun Jeong Lee / Luis R. Saraiva / Naresh K. Hanchate / Xiaolan Ye / Gregory Asher / Jonathan Ho / Linda B. Buck

    Scientific Reports, Vol 12, Iss 1, Pp 1-

    2022  Volume 11

    Abstract: Abstract Scents have been employed for millennia to allay stress, but whether or how they might do so is largely unknown. Fear and stress induce increases in blood stress hormones controlled by hypothalamic corticotropin releasing hormone neurons (CRHNs). ...

    Abstract Abstract Scents have been employed for millennia to allay stress, but whether or how they might do so is largely unknown. Fear and stress induce increases in blood stress hormones controlled by hypothalamic corticotropin releasing hormone neurons (CRHNs). Here, we report that two common odorants block mouse stress hormone responses to three potent stressors: physical restraint, predator odor, and male–male social confrontation. One odorant inhibits restraint and predator odor activation of excitatory neurons upstream of CRHNs in the bed nucleus of the stria terminalis (BNSTa). In addition, both activate inhibitory neurons upstream of CRHNs in the hypothalamic ventromedial nucleus (VMH) and silencing of VMH inhibitory neurons hinders odor blocking of stress. Together, these findings indicate that odor blocking can occur via two mechanisms: (1) Inhibition of excitatory neurons that transmit stress signals to CRHNs and (2) activation of inhibitory neurons that act directly or indirectly to inhibit stressor activation of CRHNs.
    Keywords Medicine ; R ; Science ; Q
    Subject code 590
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Detection of cathinone and mephedrone in plasma by LC-MS/MS using standard addition quantification technique

    Shu-Yuan Cheng / Theron Ng-A-Qui / Bruce Eng / Jonathan Ho

    Journal of Analytical Science and Technology, Vol 8, Iss 1, Pp 1-

    2017  Volume 6

    Abstract: Abstract Background Cathinones better known as bath salts have been listed as illicit drugs since 2011. Few studies have focused on the analytical extraction techniques and the matrix effect affecting their detection and quantification in biological ... ...

    Abstract Abstract Background Cathinones better known as bath salts have been listed as illicit drugs since 2011. Few studies have focused on the analytical extraction techniques and the matrix effect affecting their detection and quantification in biological samples. Matrix suppression of the signal of cathinones has been previously observed in urine sample by LC-MS/MS. This study is aimed to use the standard addition method to overcome the plasma matrix effect on the quantification of cathinone and mephedrone by LC-MS/MS. Findings The results showed the matrix effect for cathinone at lowest tested concentration (10 ng/ml) was significantly reduced from 210.9 to 133.7%, but not for mephedrone (from 196.8 to 191.9%) by using standard addition method. At the higher tested concentrations of samples, the matrix effects were significantly reduced for both cathinone and mephedrone by using standard addition technique. Conclusions Standard addition quantitative technique can serve as an alternative quantitative method for LC-MS/MS when suitable internal standards are not available.
    Keywords Cathinone ; Mephedrone ; LC-MS/MS ; Plasma ; Standard addition ; Chemistry ; QD1-999 ; Analytical chemistry ; QD71-142
    Subject code 621
    Language English
    Publishing date 2017-09-01T00:00:00Z
    Publisher SpringerOpen
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Sustainable nano-sodium silicate and silver nitrate impregnated locally made ceramic filters for point-of-use water treatments in sub-Sahara African households.

    Maxwell, Omeje / Oghenerukevwe, Orere Faith / Adewoyin Olusegun, O / Joel, Emmanuel Sunday / Daniel, Ozieme Arinze / Oluwasegun, Ayanbisi / Jonathan, Hassana O / Samson, Taiwo O / Adeleye, Ngozi / Michael, Orosun Muyiwa / Omeje Uchechukwu, A / Akinwumi Oluwasayo, A / Akinpelu, Akinwumi / L, Akinyemi M / Oladokun, Olagoke

    Heliyon

    2021  Volume 7, Issue 12, Page(s) e08470

    Abstract: The poor access to water quality for Nigerians has pushed for the designing of new trend silver nitrate impregnated locally made Point-Of-Use (POU) ceramic filters to enhance water purification efficiency for household use. This study utilized silver ... ...

    Abstract The poor access to water quality for Nigerians has pushed for the designing of new trend silver nitrate impregnated locally made Point-Of-Use (POU) ceramic filters to enhance water purification efficiency for household use. This study utilized silver nitrate-molded ceramic filters prepared with Kaolin from Owode, silt soil, sodium silicate, sawdust, and distilled water in three varying proportions to ascertain pollution removal efficiencies. Heating was carried out by firing the filters at 900 °C and further preheating at 400 °C after dipping in silver nitrate solution. Silver nanoparticle and dissociated particle discharge from filter pot painted with 0.03 mg/g casein-covered nAg or AgNO
    Language English
    Publishing date 2021-11-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2021.e08470
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: NAD+ Repletion Rescues Female Fertility during Reproductive Aging

    Michael J. Bertoldo / Dave R. Listijono / Wing-Hong Jonathan Ho / Angelique H. Riepsamen / Dale M. Goss / Dulama Richani / Xing L. Jin / Saabah Mahbub / Jared M. Campbell / Abbas Habibalahi / Wei-Guo Nicholas Loh / Neil A. Youngson / Jayanthi Maniam / Ashley S.A. Wong / Kaisa Selesniemi / Sonia Bustamante / Catherine Li / Yiqing Zhao / Maria B. Marinova /
    Lynn-Jee Kim / Laurin Lau / Rachael M. Wu / A. Stefanie Mikolaizak / Toshiyuki Araki / David G. Le Couteur / Nigel Turner / Margaret J. Morris / Kirsty A. Walters / Ewa Goldys / Christopher O’Neill / Robert B. Gilchrist / David A. Sinclair / Hayden A. Homer / Lindsay E. Wu

    Cell Reports, Vol 30, Iss 6, Pp 1670-1681.e

    2020  Volume 7

    Abstract: Summary: Reproductive aging in female mammals is an irreversible process associated with declining oocyte quality, which is the rate-limiting factor to fertility. Here, we show that this loss of oocyte quality with age accompanies declining levels of the ...

    Abstract Summary: Reproductive aging in female mammals is an irreversible process associated with declining oocyte quality, which is the rate-limiting factor to fertility. Here, we show that this loss of oocyte quality with age accompanies declining levels of the prominent metabolic cofactor nicotinamide adenine dinucleotide (NAD+). Treatment with the NAD+ metabolic precursor nicotinamide mononucleotide (NMN) rejuvenates oocyte quality in aged animals, leading to restoration in fertility, and this can be recapitulated by transgenic overexpression of the NAD+-dependent deacylase SIRT2, though deletion of this enzyme does not impair oocyte quality. These benefits of NMN extend to the developing embryo, where supplementation reverses the adverse effect of maternal age on developmental milestones. These findings suggest that late-life restoration of NAD+ levels represents an opportunity to rescue female reproductive function in mammals. : Declining oocyte quality is considered an irreversible feature of aging and is rate limiting for human fertility. Bertoldo et al. show that reversing an age-dependent decline in NAD(P)H restores oocyte quality, embryo development, and functional fertility in aged mice. These findings may be relevant to reproductive medicine. Keywords: oocyte, nicotinamide adenine dinucleotide (NAD+), nicotinamide mononucleotide (NMN), SIRT2, female fertility, infertility, reproductive aging, aging, embryo development, in vitro fertilization
    Keywords Biology (General) ; QH301-705.5
    Subject code 336
    Language English
    Publishing date 2020-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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