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  1. Article ; Online: Radiofrequency septal reduction in symptomatic hypertrophic obstructive cardiomyopathy.

    Crossen, Karl / Jones, Marsha / Erikson, Christopher

    Heart rhythm

    2016  Volume 13, Issue 9, Page(s) 1885–1890

    Abstract: Background: Alcohol septal ablation remains the only approved nonsurgical therapeutic alternative for patients with drug-resistant hypertrophic obstructive cardiomyopathy (HOCM). Radiofrequency (RF) ablation offers another option for treating HOCM.: ... ...

    Abstract Background: Alcohol septal ablation remains the only approved nonsurgical therapeutic alternative for patients with drug-resistant hypertrophic obstructive cardiomyopathy (HOCM). Radiofrequency (RF) ablation offers another option for treating HOCM.
    Objective: The purpose of this study was to determine whether irrigated RF ablation can reduce symptomatic outflow tract obstruction in adults with HOCM.
    Methods: Patients with symptomatic HOCM and an outflow gradient of >50 mm Hg despite medication were offered RF ablation. In 11 patients, the hypertrophied interventricular septum was localized on a 3-dimensional mapping system and ablated via a transmitral or retrograde aortic approach.
    Results: Ten of 11(91%) patients had a significant and persistent reduction in resting and provocable left ventricular outflow tract gradients. Mean resting gradients at 12 months postprocedure were reduced by 85% (66.7 mm Hg at baseline to 10.0 mm Hg at 12 months); mean provocable gradients were reduced by 85% from baseline (136.2 mm Hg at baseline to 20.0 mm Hg at 12 months). Functional New York Heart Association heart classification improved from class 3.0 ± 0.0 in all patients to class mean of 1.8 ± 0.8.
    Conclusion: RF septal ablation for the treatment of HOCM is a safe therapeutic option that allows for significant reduction in left ventricular outflow tract gradients, improvement in symptoms, and increase in efficacy rates comparable to reported rates for alcohol septal ablation.
    Language English
    Publishing date 2016-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2229357-7
    ISSN 1556-3871 ; 1547-5271
    ISSN (online) 1556-3871
    ISSN 1547-5271
    DOI 10.1016/j.hrthm.2016.04.018
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Keratinocytes as modulators of sensory afferent firing.

    Ritter-Jones, Marsha / Najjar, Sarah / Albers, Kathryn M

    Pain

    2016  Volume 157, Issue 4, Page(s) 786–787

    MeSH term(s) Afferent Pathways/physiology ; Humans ; Keratinocytes/cytology ; Neurons, Afferent/cytology ; Skin/cytology ; Skin Physiological Phenomena
    Language English
    Publishing date 2016-02-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 193153-2
    ISSN 1872-6623 ; 0304-3959
    ISSN (online) 1872-6623
    ISSN 0304-3959
    DOI 10.1097/j.pain.0000000000000490
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Intrathecal cannabinoid-1 receptor agonist prevents referred hyperalgesia in acute acrolein-induced cystitis in rats.

    Jones, Marsha Ritter / Wang, Zun-Yi / Bjorling, Dale E

    American journal of clinical and experimental urology

    2015  Volume 3, Issue 1, Page(s) 28–35

    Abstract: We investigated the capacity of intrathecal arachidonyl-2'-chloroethylamide (ACEA), a cannabinoid-1 receptor (CB1R) agonist, to inhibit referred hyperalgesia and increased bladder contractility resulting from acute acrolein-induced cystitis in rats. 24 ... ...

    Abstract We investigated the capacity of intrathecal arachidonyl-2'-chloroethylamide (ACEA), a cannabinoid-1 receptor (CB1R) agonist, to inhibit referred hyperalgesia and increased bladder contractility resulting from acute acrolein-induced cystitis in rats. 24 female rats were divided into 4 groups: 1) intrathecal vehicle/intravesical saline; 2) intrathecal vehicle/intravesical acrolein; 3) intrathecal ACEA/intravesical saline; and 4) intrathecal ACEA/intravesical acrolein. Bladder catheters were placed 4-6 days prior to the experiment. On the day of the experiment, rats were briefly anesthetized with isoflurane to recover the external end of the cystostomy catheter. After recovery from anesthesia, pre-treatment cystometry was performed, and mechanical sensitivity of the hindpaws was determined. Rats were again briefly anesthetized with isoflurane to inject ACEA or vehicle into the intrathecal space between L5-L6. Beginning 10 minutes after intrathecal injection, saline or acrolein was infused into the bladder for 30 minutes. Post-treatment cystometry and mechanical sensitivity testing were performed. Rats were euthanized, and bladders were collected, weighed, and fixed for histology. The intrathecal vehicle/intravesical acrolein group developed mechanical hyperalgesia with post-treatment mechanical sensitivity of 6 ± 0.3 g compared to pretreatment of 14 ± 0.4 g (p < 0.01). Pre- and post-treatment hind paw mechanical sensitivity was statistically similar in rats that received intrathecal ACEA prior to intravesical infusion of acrolein (15 ± 0.2 g and 14 ± 0.4 g, respectively). Acrolein treatment increased basal bladder pressure and maximal voiding pressure and decreased intercontraction interval and voided volume. However, intrathecal ACEA was ineffective in improving acrolein-related urodynamic changes. In addition, bladder histology demonstrated submucosal and muscularis edema that was similar for all acrolein-treated groups, irrespective of ACEA treatment. Intravesical saline had no effect on results of cystometry or mechanical sensitivity of the hind paws, regardless of intrathecal treatment. Intrathecal ACEA prevented referred hyperalgesia associated with acute acrolein-induced cystitis. However, in this experimental model, ACEA did not ameliorate the associated urodynamic changes. These findings suggest that pain arising from cystitis may be inhibited by activation of spinal CB1R but the acute local response of the bladder appeared to be unaffected by stimulation of spinal CB1R.
    Language English
    Publishing date 2015-04-25
    Publishing country United States
    Document type Journal Article
    ISSN 2330-1910
    ISSN 2330-1910
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Cutaneous TRPV1

    Cohen, Jonathan A / Edwards, Tara N / Liu, Andrew W / Hirai, Toshiro / Jones, Marsha Ritter / Wu, Jianing / Li, Yao / Zhang, Shiqun / Ho, Jonhan / Davis, Brian M / Albers, Kathryn M / Kaplan, Daniel H

    Cell

    2019  Volume 178, Issue 4, Page(s) 919–932.e14

    Abstract: Cutaneous ... ...

    Abstract Cutaneous TRPV1
    MeSH term(s) Animals ; Candida albicans/immunology ; Immunity, Innate/immunology ; Inflammation/immunology ; Interleukin-23/metabolism ; Interleukin-6/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Optogenetics/methods ; Sensory Receptor Cells/immunology ; Skin/immunology ; Skin/microbiology ; Staphylococcus aureus/immunology ; TRPV Cation Channels/genetics ; TRPV Cation Channels/metabolism ; Tumor Necrosis Factor-alpha/metabolism
    Chemical Substances Interleukin-23 ; Interleukin-6 ; TRPV Cation Channels ; TRPV1 protein, mouse ; Tumor Necrosis Factor-alpha ; interleukin-6, mouse
    Language English
    Publishing date 2019-07-25
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2019.06.022
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Cutaneous TRPV1+ Neurons Trigger Protective Innate Type 17 Anticipatory Immunity

    Cohen, Jonathan A / Edwards, Tara N / Liu, Andrew W / Hirai, Toshiro / Jones, Marsha Ritter / Wu, Jianing / Li, Yao / Zhang, Shiqun / Ho, Jonhan / Davis, Brian M / Albers, Kathryn M / Kaplan, Daniel H

    Cell. 2019 Aug. 08, v. 178, no. 4

    2019  

    Abstract: Cutaneous TRPV1+ neurons directly sense noxious stimuli, inflammatory cytokines, and pathogen-associated molecules and are required for innate immunity against some skin pathogens. Important unanswered questions are whether TRPV1+ neuron activation in ... ...

    Abstract Cutaneous TRPV1+ neurons directly sense noxious stimuli, inflammatory cytokines, and pathogen-associated molecules and are required for innate immunity against some skin pathogens. Important unanswered questions are whether TRPV1+ neuron activation in isolation is sufficient to initiate innate immune responses and what is the biological function for TRPV1+ neuron-initiated immune responses. We used TRPV1-Ai32 optogenetic mice and cutaneous light stimulation to activate cutaneous neurons in the absence of tissue damage or pathogen-associated products. We found that TRPV1+ neuron activation was sufficient to elicit a local type 17 immune response that augmented host defense to C. albicans and S. aureus. Moreover, local neuron activation elicited type 17 responses and augmented host defense at adjacent, unstimulated skin through a nerve reflex arc. These data show the sufficiency of TRPV1+ neuron activation for host defense and demonstrate the existence of functional anticipatory innate immunity at sites adjacent to infection that depends on antidromic neuron activation.
    Keywords Candida albicans ; Staphylococcus aureus ; cytokines ; immune response ; innate immunity ; mice ; nerve tissue ; neurons ; optogenetics ; pathogens
    Language English
    Dates of publication 2019-0808
    Size p. 919-932.e14.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2019.06.022
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Method for screening and MALDI-TOF MS sequencing of encoded combinatorial libraries.

    Hu, Bi-Huang / Jones, Marsha Ritter / Messersmith, Phillip B

    Analytical chemistry

    2007  Volume 79, Issue 19, Page(s) 7275–7285

    Abstract: We describe a new method for encoded synthesis, efficient on-resin screening, and rapid unambiguous sequencing of combinatorial peptide libraries. An improved binary tag system for encoding peptide libraries during synthesis was designed to facilitate ... ...

    Abstract We describe a new method for encoded synthesis, efficient on-resin screening, and rapid unambiguous sequencing of combinatorial peptide libraries. An improved binary tag system for encoding peptide libraries during synthesis was designed to facilitate unequivocal assignment of isobaric residues by MALDI-TOF MS analysis. The improved method for encoded library synthesis was combined with a new versatile on-resin screening strategy that permitted multiple stages and types of screening to be employed successively on one library under mild conditions. The new method facilitated a combinatorial study of transglutaminase (TGase) enzyme substrate peptides, revealing new details of the effect of amino acid composition on TGase substrates. The approach was first demonstrated for an encoded library (130,321 compounds) of lysine pentapeptide substrates of TGase, synthesized using the "split-mix" method. The library was reacted on-resin with TGase enzyme and a soluble desthiobiotin labeled glutamine substrate. Initial screening was performed by adsorbing streptavidin-coated magnetic microparticles onto library beads, followed by magnetic separation. The differential binding affinities of desthiobiotin and biotin for streptavidin were exploited to release the magnetic microparticles and regenerate the desthiobiotin-labeled resin beads for further screening by flow-cytometry-based automated bead sorting, resulting in 345 beads that were sequenced by MALDI-TOF MS analysis. A second library consisted of encoded glutamine hexapeptide substrates, which was reacted on-resin with TGase enzyme and a soluble desthiobiotin-labeled cadaverine. Two-stage screening identified 267 glutamine peptides as TGase-reactive, of which 21 were further analyzed by solution-phase enzyme kinetics. Kinetic results indicated that the peptide PQQQYV from the library has a 68-fold greater substrate specificity than the best known glutamine substrate QQIV. The new encoding and screening strategies described here are expected to be broadly applicable to synthesis and screening of combinatorial peptide libraries in the future.
    MeSH term(s) Combinatorial Chemistry Techniques ; Magnetics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
    Language English
    Publishing date 2007-10-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/ac070418g
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Facile coupling of synthetic peptides and peptide-polymer conjugates to cartilage via transglutaminase enzyme.

    Jones, Marsha Elizabeth Ritter / Messersmith, Phillip B

    Biomaterials

    2007  Volume 28, Issue 35, Page(s) 5215–5224

    Abstract: Covalent attachment of synthetic and biological molecules to tissue surfaces can be used to enhance local drug delivery, reduce adhesions after surgery, and attach reconstructive biomaterials and tissue-engineered matrices to tissues. We present here a ... ...

    Abstract Covalent attachment of synthetic and biological molecules to tissue surfaces can be used to enhance local drug delivery, reduce adhesions after surgery, and attach reconstructive biomaterials and tissue-engineered matrices to tissues. We present here a mild approach to coupling polymers to tissue surfaces through an enzyme catalyzed reaction between peptide modified polymer and native protein components of the tissue extracellular matrix (ECM). Tissue transglutaminase (tTG), a Ca2+-dependent enzyme that catalyzes the reaction between lysine and glutamine residues to form a epsilon(gamma-glutaminyl) lysine isopeptide bond, was incubated with cartilage in the presence of lysine (FKG-NH2) and glutamine (GQQQLG-NH2) peptides as well as peptide functionalized poly(ethylene glycol) (PEG). Immunohistochemistry was used to detect the presence of covalently bound PEG polymer at the tissue surface as well as to a depth of as much as 10 microm below the surface. Collagen II, fibronectin, osteopontin and osteonectin were found to react with the peptides and peptide modified PEG in the presence of tTG in solution, suggesting these cartilage ECM components as being substrates in the tissue reaction. The results illustrate the use of tTG as a simple, effective and biologically compatible method of coupling synthetic and biological molecules to cartilage and other tissues containing ECM proteins that are substrates of tTG.
    MeSH term(s) Animals ; Biocompatible Materials/metabolism ; Cartilage, Articular/enzymology ; Cartilage, Articular/metabolism ; Cattle ; Extracellular Matrix/enzymology ; Extracellular Matrix/metabolism ; GTP-Binding Proteins/physiology ; Guinea Pigs ; Peptides/chemical synthesis ; Peptides/metabolism ; Transglutaminases/physiology
    Chemical Substances Biocompatible Materials ; Peptides ; transglutaminase 2 (EC 2.3.2.-) ; Transglutaminases (EC 2.3.2.13) ; GTP-Binding Proteins (EC 3.6.1.-)
    Language English
    Publishing date 2007-12
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 603079-8
    ISSN 0142-9612
    ISSN 0142-9612
    DOI 10.1016/j.biomaterials.2007.08.026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: In situ forming biomaterials.

    Ritter Jones, Marsha / Messersmith, Phillip B

    Oral and maxillofacial surgery clinics of North America

    2007  Volume 14, Issue 1, Page(s) 29–38

    Language English
    Publishing date 2007-12-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1316546-x
    ISSN 1558-1365 ; 1042-3699
    ISSN (online) 1558-1365
    ISSN 1042-3699
    DOI 10.1016/s1042-3699(02)00015-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: The American Heart Association 2030 Impact Goal: A Presidential Advisory From the American Heart Association.

    Angell, Sonia Y / McConnell, Michael V / Anderson, Cheryl A M / Bibbins-Domingo, Kirsten / Boyle, Douglas S / Capewell, Simon / Ezzati, Majid / de Ferranti, Sarah / Gaskin, Darrell J / Goetzel, Ron Z / Huffman, Mark D / Jones, Marsha / Khan, Yosef M / Kim, Sonia / Kumanyika, Shiriki K / McCray, Alexa T / Merritt, Robert K / Milstein, Bobby / Mozaffarian, Dariush /
    Norris, Tyler / Roth, Gregory A / Sacco, Ralph L / Saucedo, Jorge F / Shay, Christina M / Siedzik, David / Saha, Somava / Warner, John J

    Circulation

    2020  Volume 141, Issue 9, Page(s) e120–e138

    Abstract: Each decade, the American Heart Association (AHA) develops an Impact Goal to guide its overall strategic direction and investments in its research, quality improvement, advocacy, and public health programs. Guided by the AHA's new Mission Statement, to ... ...

    Abstract Each decade, the American Heart Association (AHA) develops an Impact Goal to guide its overall strategic direction and investments in its research, quality improvement, advocacy, and public health programs. Guided by the AHA's new Mission Statement, to be a relentless force for a world of longer, healthier lives, the 2030 Impact Goal is anchored in an understanding that to achieve cardiovascular health for all, the AHA must include a broader vision of health and well-being and emphasize health equity. In the next decade, by 2030, the AHA will strive to equitably increase healthy life expectancy beyond current projections, with global and local collaborators, from 66 years of age to at least 68 years of age across the United States and from 64 years of age to at least 67 years of age worldwide. The AHA commits to developing additional targets for equity and well-being to accompany this overarching Impact Goal. To attain the 2030 Impact Goal, we recommend a thoughtful evaluation of interventions available to the public, patients, providers, healthcare delivery systems, communities, policy makers, and legislators. This presidential advisory summarizes the task force's main considerations in determining the 2030 Impact Goal and the metrics to monitor progress. It describes the aspiration that these goals will be achieved by working with a diverse community of volunteers, patients, scientists, healthcare professionals, and partner organizations needed to ensure success.
    MeSH term(s) Aged ; American Heart Association ; Cardiovascular Diseases/diagnosis ; Cardiovascular Diseases/epidemiology ; Cardiovascular Diseases/mortality ; Cardiovascular Diseases/prevention & control ; Global Health ; Health Status ; Humans ; Middle Aged ; Policy Making ; Population Surveillance ; Preventive Health Services/standards ; Risk Assessment ; Risk Factors ; Time Factors ; United States/epidemiology
    Language English
    Publishing date 2020-01-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80099-5
    ISSN 1524-4539 ; 0009-7322 ; 0069-4193 ; 0065-8499
    ISSN (online) 1524-4539
    ISSN 0009-7322 ; 0069-4193 ; 0065-8499
    DOI 10.1161/CIR.0000000000000758
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Thermal gelation and tissue adhesion of biomimetic hydrogels.

    Burke, Sean A / Ritter-Jones, Marsha / Lee, Bruce P / Messersmith, Phillip B

    Biomedical materials (Bristol, England)

    2007  Volume 2, Issue 4, Page(s) 203–210

    Abstract: Marine and freshwater mussels are notorious foulers of natural and manmade surfaces, secreting specialized protein adhesives for rapid and durable attachment to wet substrates. Given the strong and water-resistant nature of mussel adhesive proteins, ... ...

    Abstract Marine and freshwater mussels are notorious foulers of natural and manmade surfaces, secreting specialized protein adhesives for rapid and durable attachment to wet substrates. Given the strong and water-resistant nature of mussel adhesive proteins, significant potential exists for mimicking their adhesive characteristics in bioinspired synthetic polymer materials. An important component of these proteins is L-3,4-dihydroxylphenylalanine (DOPA), an amino acid believed to contribute to mussel glue solidification through oxidation and crosslinking reactions. Synthetic polymers containing DOPA residues have previously been shown to crosslink into hydrogels upon the introduction of oxidizing reagents. Here we introduce a strategy for stimuli responsive gel formation of mussel adhesive protein mimetic polymers. Lipid vesicles with a bilayer melting transition of 37 degrees C were designed from a mixture of dipalmitoyl and dimyristoyl phosphatidylcholines and exploited for the release of a sequestered oxidizing reagent upon heating from ambient to physiologic temperature. Colorimetric studies indicated that sodium-periodate-loaded liposomes released their cargo at the phase transition temperature, and when used in conjunction with a DOPA-functionalized poly(ethylene glycol) polymer gave rise to rapid solidification of a crosslinked polymer hydrogel. The tissue adhesive properties of this biomimetic system were determined by in situ thermal gelation of liposome/polymer hydrogel between two porcine dermal tissue surfaces. Bond strength measurements showed that the bond formed by the adhesive hydrogel (mean = 35.1 kPa, SD = 12.5 kPa, n = 11) was several times stronger than a fibrin glue control tested under the same conditions. The results suggest a possible use of this biomimetic strategy for repair of soft tissues.
    MeSH term(s) Adhesiveness ; Animals ; Biomimetic Materials/chemistry ; Cell Adhesion/physiology ; Dihydroxyphenylalanine/chemistry ; Hot Temperature ; Hydrogels/chemistry ; In Vitro Techniques ; Lipid Bilayers/chemistry ; Materials Testing ; Phospholipids/chemistry ; Skin Physiological Phenomena ; Swine ; Tissue Adhesives/chemistry
    Chemical Substances Hydrogels ; Lipid Bilayers ; Phospholipids ; Tissue Adhesives ; Dihydroxyphenylalanine (63-84-3)
    Language English
    Publishing date 2007-09-24
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2265222-X
    ISSN 1748-605X ; 1748-6041
    ISSN (online) 1748-605X
    ISSN 1748-6041
    DOI 10.1088/1748-6041/2/4/001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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