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  1. Article ; Online: Describing the intestinal microbiota of Holstein Fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central Colombia.

    Angie L Ramírez / Giovanny Herrera / Marina Muñoz / Laura Vega / Lissa Cruz-Saavedra / Diego García-Corredor / Martin Pulido-Medellín / Diana M Bulla-Castañeda / Julio Cesar Giraldo / María Consuelo Bernal / José Fernández-Manrique / Luis R Vásquez-Arteaga / Juan David Ramírez

    PLoS Neglected Tropical Diseases, Vol 15, Iss 8, p e

    2021  Volume 0009658

    Abstract: The ability to identify compositional changes in the intestinal microbiota of parasitized hosts is important for understanding the physiological processes that may affect animal productivity. Within the field of host-parasite interactions, many studies ... ...

    Abstract The ability to identify compositional changes in the intestinal microbiota of parasitized hosts is important for understanding the physiological processes that may affect animal productivity. Within the field of host-parasite interactions, many studies have suggested that helminths can influence the microbial composition of their hosts via their immunomodulatory effects. Bovine fascioliasis is a helminthiasis widely studied by immunologists, but with little information available regarding gut microbial communities. Thus, we aimed to describe the composition of the intestinal microbiota of Holstein Fasciola-positive and -negative cattle using parasitological methods and ELISA (enzyme-linked immunosorbent assay). Bovine fecal samples (n = 65) were obtained from livestock slaughter plants in the Cundi-Boyacense Colombian highlands (a hyperendemic region for bovine fascioliasis) and studied by amplicon-based next-generation 16S-rRNA and 18S-rRNA gene sequencing. From these samples, 35 were Fasciola hepatica-negative and, 30 were F. hepatica-positive in our detection analysis. Our results showed a reduction in the relative abundance of Bacteroidetes and Ascomycota in the Fasciola-positive samples, along with decreased relative abundances of the commensal taxa previously associated with fermentation and digestion processes. However, metabolomic approaches and functional analyzes of the intestinal microbiota are necessary to support these hypothesis. These findings are a small first step in the development of research aimed at understanding how microbial populations in bovines are modulated in liver helminth infections.
    Keywords Arctic medicine. Tropical medicine ; RC955-962 ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2021-08-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Sinópsis de la Evaluación de un Método de Extracción de Semen y Morfometría Espermática de Lapa Cuniculus paca en un Núcleo de Reubicación de Cormacarena

    J Cuan-Barrera / Jessica Gómez-Guerrero / C Parra-Sandoval / Ricardo Murillo-Pacheco / José Fernández-Manrique

    Memorias de la Conferencia Interna en Medicina y Aprovechamiento de Fauna Silvestre, Exótica y no Convencional, Vol 9, Iss 1, Pp 15-

    2013  Volume 16

    Abstract: La lapa (Cuniculus paca) es un roedor utilizado como fuente de proteína en la Orinoquia. La Corporación Autónoma Regional de Macarena (CORMACARENA) viene promocionando zoocriaderos no comerciales con el propósito de disminuir la cacería y prevenir la ... ...

    Abstract La lapa (Cuniculus paca) es un roedor utilizado como fuente de proteína en la Orinoquia. La Corporación Autónoma Regional de Macarena (CORMACARENA) viene promocionando zoocriaderos no comerciales con el propósito de disminuir la cacería y prevenir la extinción. Teniendo en cuenta el decreto 309 del 2000 del Ministerio de Ambiente sobre investigación con diversidad biológica y la Ley 84 de 1989 Estatuto sobre la protección de los animales en Colombia, el objetivo de este trabajo fue determinar la efectividad del método de obtención de semen directamente de la cola del epidídimo. Un macho adulto de 8 Kg de peso, proveniente del núcleo de reubicación El Turpial, vereda Brisas del Ocoa, municipio de Villavicencio, animal con historia de agresividad con sus congéneres y cuidadores, se le aplicó protocolo anestésico, atropina (0,044 mg/kg) y Zoletil® 50 (5 mg/kg), y se realizó orquiectomia.
    Keywords Cuniculus paca ; orquiectomía ; semen epididimal ; aspirado seminal ; Animal culture ; SF1-1100 ; Agriculture ; S
    Language Spanish
    Publishing date 2013-08-01T00:00:00Z
    Publisher Asociacion de Veterinarios de Vida Silvestre
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Evaluacion por Western Blot, Inmunofluorescencia Indirecta y ELISA de Perros Infectados con Leishmania (Leishmania) infantum Western blot, ELISA and indirect immunofluorescence test evaluation of Leishmania (Leishmania) infantum-infected dogs

    Jimmy J Vargas-Duarte / Myriam C López-Páez / Jesús E Escovar-Castro / José Fernández-Manrique

    Revista de Salud Pública, Vol 11, Iss 4, Pp 641-

    2009  Volume 652

    Abstract: Objetivo Evaluar el desempeño de las pruebas empleadas en Colombia para el diagnóstico de la leishmaniasis visceral canina y adaptar una técnica de Western blot empleando animales experimental y naturalmente infectados. Metodología Se obtuvieron sueros ... ...

    Abstract Objetivo Evaluar el desempeño de las pruebas empleadas en Colombia para el diagnóstico de la leishmaniasis visceral canina y adaptar una técnica de Western blot empleando animales experimental y naturalmente infectados. Metodología Se obtuvieron sueros de 10 perros infectados experimentalmente con L. infantum, 5 perros infectados naturalmente, 16 perros sanos, 26 de reacción cruzada (infectados con Babesia canis, Erhlichia canis, Dirofilaria immitis, Trypanosoma cruzi, Leishmania (Viannia) spp.), 40 de zonas no endémicas y 150 de zona endémica. Todos fueron evaluados mediante las pruebas de inmunofluorescencia indirecta (IFI), ELISA y Western blot (WB). Resultados Se encontró que IFI tuvo el mayor porcentaje de positividad en los perros infectados (73 %) mientras que el menor porcentaje de falsos positivos se obtuvo por WB (2,5 %). La prueba de ELISA fue la menos eficiente. Fueron reconocidas 24 fracciones antigénicas, las bandas de 29, 34, 50, 69, 75, 86, 99 y 123 kDa fueron responsables de reacciones inespecíficas en los sueros de perros sanos, de zona no endémica y de reacción cruzada. Las bandas por debajo de 29 kDa mostraron ser potencialmente diagnósticas, especialmente la fracción de 13 kDa. Conclusiones Los métodos directos y serológicos pueden subdiagnosticar la infección por Leishmania, solamente un constructo que combine tanto pruebas directas como indirectas sería la forma más eficiente de diagnóstico. Objective Evaluating canine visceral leishmaniasis diagnostic test performance in Colombia and adapting the Western blot test in naturally and experimentally infected dogs. Methods Sera were obtained from 10 experimentally L. Infantum-infected dogs, 5 naturally infected dogs, 16 healthy dogs, 26 Babesia canis, Erhlichia canis, Dirofilaria immitis, Trypanosoma cruzi and Leishmania (Viannia) spp infected dogs, 40 dogs from non-endemic areas and 150 from endemic areas. Sera were tested for L. infantum infection using immunofluorescent antibody (IFAT), ELISA and Western blot (WB) tests. Results Positives results were obtained for 73 % of known infected dogs by the IFAT test and false positives were obtained for 2.5 % of non-infected dogs using WB. ELISA was not efficient for diagnosis. 24 antigenic fractions were recognised in tested sera using WB; however, 29, 34, 50, 69, 75, 86, 99 and 123 kDa bands were recognised in sera from dogs from non-endemic areas, healthy dogs and Trypanosoma cruzi, Erhlichia canis, Dirofilaria immitis and Babesia canis infected dogs. The 13 kDa fraction proved potentially useful for diagnosing canine visceral leishmaniasis. ConclusionsThe separate use of parasitological and serological test could lead to misdiagnosis of Leishmania infection; using both kinds of technique simultaneously is thus highly recommended.
    Keywords Leishmaniasis Visceral ; Western blotting ; prueba ELISA ; pruebas inmunológicas ; reservorios ; Leishmaniasis ; immunoblotting ; enzyme-linked immunosorbent assay (ELISA) ; fluorescent antibody technique ; immunological test ; reservoir ; Public aspects of medicine ; RA1-1270 ; Medicine ; R ; DOAJ:Public Health ; DOAJ:Health Sciences
    Publishing date 2009-08-01T00:00:00Z
    Publisher Instituto de Salud Publica, Facultad de Medicina - Universidad Nacional de Colombia
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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