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  1. Article ; Online: Optimization of Plant Production by Seed Treatment in Two Wild Subspecies of Narcissus pseudonarcissus Rich in Alkaloids

    Raquel Herranz / Miguel A. Copete / José M. Herranz / Elena Copete / Pablo Ferrandis

    Molecules, Vol 25, Iss 4439, p

    2020  Volume 4439

    Abstract: The daffodil Narcissus pseudonarcissus L. contains alkaloids of pharmaceutical interest. Wild daffodil populations have diverse genetic backgrounds and various genetic traits of possible importance. Developing protocols for plant production from seeds ... ...

    Abstract The daffodil Narcissus pseudonarcissus L. contains alkaloids of pharmaceutical interest. Wild daffodil populations have diverse genetic backgrounds and various genetic traits of possible importance. Developing protocols for plant production from seeds may ensure the availability of a large reservoir of individuals as well as being important for species with bulbs that are difficult to acquire. The closely related Narcissus pseudonarcissus subsp. munozii-garmendiae and subsp. nevadensis were investigated in this study because the alkaloids isolated from both are of high pharmacological interest. At the dispersal time, the seeds of both were dormant with underdeveloped embryos, i.e., morphophysiological dormancy (MPD). Experiments were conducted outdoors and under controlled laboratory conditions. Embryo growth and the percentages of radicle and seedling emergence were calculated under different temperature–light stratifications. In N. munozii-garmendiae , embryo growth occurred during warm stratification (28/14 °C or 25/10 °C) and the radicle then emerged when the temperature decreased, but the shoot was dormant. In N. nevadensis , the seeds germinated when cold stratified (5 °C) and then incubated at cool temperatures. Thus, N. munozii-garmendiae and N. nevadensis exhibit different levels of MPD, i.e., deep simple epicotyl and intermediate complex, respectively. Plant production protocols from seeds were established for both taxa in this study.
    Keywords alkaloid ; Amaryllidaceae ; deep simple epicotyl morphophysiological dormancy ; dormancy breakage ; embryo growth ; germination ; Organic chemistry ; QD241-441
    Subject code 580
    Language English
    Publishing date 2020-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Análisis comparativo del área y morfología foliar en taxones ibéricos amenazados del género Coincya (Cruciferae) Comparative analysis of leaf area and morphology in threatened Iberian Coincya (Cruciferae) taxa

    MIGUEL A COPETE / JOSÉ M HERRANZ / PABLO FERRANDIS

    Revista Chilena de Historia Natural, Vol 83, Iss 3, Pp 365-

    2010  Volume 374

    Abstract: Se evalúa la variación del parámetro área foliar y un índice de morfología foliar (índice de forma) en plantas de 1, 2 y 3 meses de edad pertenecientes a cuatro taxones del género Coincya (Cruciferae) relacionados filogenéticamente. Para todas las edades ...

    Abstract Se evalúa la variación del parámetro área foliar y un índice de morfología foliar (índice de forma) en plantas de 1, 2 y 3 meses de edad pertenecientes a cuatro taxones del género Coincya (Cruciferae) relacionados filogenéticamente. Para todas las edades el área foliar alcanzó sus valores más altos en las formas que tienen frutos con rostros gruesos (C. rupestris), aumentando progresivamente según el siguiente orden: C. monensis subsp. orophila, C. longirostra, C. rupestris subsp. leptocarpa y C. rupestris subsp. rupestris. Se plantea que el aumento del área foliar en los taxones cuyos frutos poseen rostros gruesos puede constituir otra ventaja adaptativa adicional a las ya mencionadas para estos en trabajos anteriores a la vez que refuerza el esquema evolutivo propuesto para la totalidad del grupo. En cuanto a la morfología foliar, el índice de forma discrimina nítidamente los taxones extremos de esta línea filogenética: C. rupestris subsp. rupestris (más evolucionado) y C monensis subsp. orophila (más arcaico) una vez alcanzada la fase adulta; las formas intermedias, representadas por C. rupestris subsp. leptocarpa y C. longirostra, quedan encuadradas en un mismo grupo casi en su totalidad. Variation in leaf area and in a leaf morphological index (shape index) was evaluated in seedlings 1, 2, and 3-month aged in four taxa of Coincya genus (Cruciferae) phylogenetically related. For all ages, leaf area reached higher values in types having thick-beak fruits (C. rupestris), and gradually increased according to the following order: C. monensis subsp. orophila, C. longirostra, C. rupestris subsp. leptocarpa, and C. rupestris subsp. rupestris. We suggest that the increase of leaf area in thick-beak taxa may be an adaptive advantage, additional to those already suggested in previous studies, reinforcing as well the evolutive schema proposed for the entire group. With regard to leaf morphology, shape index neatly discriminated extreme taxa in the phylogenetical line (i.e., C. rupestris subsp. rupestris in the most ...
    Keywords área foliar ; morfología foliar ; rostro engrosado ; ventaja adaptativa ; adaptive advantage ; leaf area ; leaf morphology ; thick fruit-beak ; Zoology ; QL1-991 ; Botany ; QK1-989
    Language English
    Publishing date 2010-01-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Reproductive biology of the critically endangered endemic Mediterranean plant Coincya rupestris subsp. rupestris (Spain)

    MIGUEL A COPETE / JOSÉ M HERRANZ / PABLO FERRANDIS

    Revista Chilena de Historia Natural, Vol 81, Iss 3, Pp 345-

    the effects of competition and summer drought on seedling establishment Biología reproductiva de la planta endémica mediterránea amenazada Coincya rupestris subsp. rupestris (España): efectos de la competencia y sequía estival en el establecimiento de plántulas

    2008  Volume 359

    Abstract: Flower, fruit, seed production, and flowering phenology (duration, intensity, moment and synchrony) were studied in the two main populations (south east Spain) of the critically endangered endemic Mediterranean plant Coincya rupestris subsp. rupestris ( ... ...

    Abstract Flower, fruit, seed production, and flowering phenology (duration, intensity, moment and synchrony) were studied in the two main populations (south east Spain) of the critically endangered endemic Mediterranean plant Coincya rupestris subsp. rupestris (Cruciferae). Production of flowers and fruits (mean ± SD) ranged from 483 (± 688) to 748 (± 636), and from 317 (± 518) to 553 (± 500), respectively, between populations. In addition, the average seed production per plant was 1,607-2,798, thus concluding that fertility was not responsible for the rarity of this taxon. The fruit/flower ratio ranged from 0.60 to 0.75, showing significant inter-population differences. Flowering extended from February-March to the end of spring, with high synchrony (= 85 %). This parameter was negatively correlated with duration of the flowering period. The role of pollinator insects on reproductive success, and the effect of watering treatments and the elimination of competitors on seedling recruitment were analysed in the classical locality. The exclusion of pollinators dramatically affected fructification, reducing reproductive success from moderate values in plants exposed to insects (= 0.5) to null values in those where insects were experimentally excluded. Seedling emergence was autumnal and no influence of the factors analysed (i.e., water availability and inter-specific competition) was detected on seedling establishment. A high interannual variability in the size and survival of cohorts originated each autumn was observed. It should be emphasized that the rarity of the taxon is not due to fecundity restrictions. Para el endemismo amenazado mediterráneo Coincya rupestris subsp. rupestris (Cruciferae) se analizaron diferentes aspectos de su biología reproductiva como la producción de flores, frutos y semillas, y la fenología de la floración a través de las variables representativas de la misma (duración, intensidad, momento y sincronía), de forma independiente en las dos poblaciones principales del taxon (sureste de España). La ...
    Keywords endemismo ibérico ; fenología de la floración ; éxito reproductivo ; reclutamiento de plántulas ; Iberian endemism ; flowering phenology ; reproductive success ; seedling recruitment ; Zoology ; QL1-991 ; Botany ; QK1-989
    Language English
    Publishing date 2008-09-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Dual Pharmacological Targeting of HDACs and PDE5 Inhibits Liver Disease Progression in a Mouse Model of Biliary Inflammation and Fibrosis

    Alex Claveria-Cabello / Leticia Colyn / Iker Uriarte / Maria Ujue Latasa / Maria Arechederra / Jose M. Herranz / Laura Alvarez / Jesus M. Urman / Maria L. Martinez-Chantar / Jesus M. Banales / Bruno Sangro / Krista Rombouts / Julen Oyarzabal / Jose J. G. Marin / Carmen Berasain / Matias A. Avila / Maite G. Fernandez-Barrena

    Cancers, Vol 12, Iss 3748, p

    2020  Volume 3748

    Abstract: Liver fibrosis, a common hallmark of chronic liver disease (CLD), is characterized by the accumulation of extracellular matrix secreted by activated hepatic fibroblasts and stellate cells (HSC). Fibrogenesis involves multiple cellular and molecular ... ...

    Abstract Liver fibrosis, a common hallmark of chronic liver disease (CLD), is characterized by the accumulation of extracellular matrix secreted by activated hepatic fibroblasts and stellate cells (HSC). Fibrogenesis involves multiple cellular and molecular processes and is intimately linked to chronic hepatic inflammation. Importantly, it has been shown to promote the loss of liver function and liver carcinogenesis. No effective therapies for liver fibrosis are currently available. We examined the anti-fibrogenic potential of a new drug (CM414) that simultaneously inhibits histone deacetylases (HDACs), more precisely HDAC1, 2, and 3 (Class I) and HDAC6 (Class II) and stimulates the cyclic guanosine monophosphate (cGMP)-protein kinase G (PKG) pathway activity through phosphodiesterase 5 (PDE5) inhibition, two mechanisms independently involved in liver fibrosis. To this end, we treated Mdr2 -KO mice, a clinically relevant model of liver inflammation and fibrosis, with our dual HDAC/PDE5 inhibitor CM414. We observed a decrease in the expression of fibrogenic markers and collagen deposition, together with a marked reduction in inflammation. No signs of hepatic or systemic toxicity were recorded. Mechanistic studies in cultured human HSC and cholangiocytes (LX2 and H69 cell lines, respectively) demonstrated that CM414 inhibited pro-fibrogenic and inflammatory responses, including those triggered by transforming growth factor β (TGFβ). Our study supports the notion that simultaneous targeting of pro-inflammatory and fibrogenic mechanisms controlled by HDACs and PDE5 with a single molecule, such as CM414, can be a new disease-modifying strategy.
    Keywords liver fibrosis ; hepatobiliary carcinogenesis ; histone deacetylases ; cGMP phosphodiesterase inhibitor ; HDAC inhibitor ; precision medicine ; Neoplasms. Tumors. Oncology. Including cancer and carcinogens ; RC254-282
    Subject code 610
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Book: Caracterización florística de los pinares naturales de Pinus pinaster Aiton en Castilla-La Mancha

    Herranz Sanz, José M / Carreño, Miguel Ángel Copete Carreño / Copete Carreño, Miguel Ángel / Herrero, Javier Martín / Sanz, José M. Herranz

    (Monografías INIA : serie forestal ; 16)

    2008  

    Abstract: Literaturverz. S. 89 - ... ...

    Author's details Autores José M. Herranz Sanz, Javier Martín Herrero, Miguel Ángel Copete Carreño
    Series title Monografías INIA : serie forestal ; 16
    Abstract Literaturverz. S. 89 - 91
    Keywords Cluster pine ; Vegetation ; Strandkiefer ; Pinienwald ; Kastilien- La Mancha
    Language Spanish
    Size 91 S., Ill., graph. Darst., Kt, 24 cm
    Document type Book
    Note Sprache der Zusammenfassung: Englisch
    ISBN 9788474985238 ; 8474985234
    Database Johann Heinrich von Thünen-Institut, Federal Research Institute for Rural Areas, Forestry and Fisheries

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  6. Article: Influence of a low-intensity fire on a Pinus halepensis Mill. forest seed bank and its consequences on the early stages of plant succession

    , PABLO FERRANDIS / , JORGE DE LAS HERAS / , JOSÉ M. HERRANZ / , JUAN J. MARTíNEZ-SÁNCHEZ / , JOSÉ M. HERRANZ

    Israel journal of plant sciences

    Volume v. 49,, Issue no. 2

    Abstract: The effect of an experimental low-intensity fire on the soil seed bank and its consequences on the early stages of post-disturbance succession were analyzed. The hypothesis that low-intensity fire may produce a population decline of hard-seeded species ... ...

    Abstract The effect of an experimental low-intensity fire on the soil seed bank and its consequences on the early stages of post-disturbance succession were analyzed. The hypothesis that low-intensity fire may produce a population decline of hard-seeded species by the elimination of established plants and the lack of a seed germination promotion effect was investigated. Consecutive pre- and post-burning soil samplings revealed extremely low impact of fire on seed banks. In contrast, some specific seed banks increased with fire, probably due to a seed rain from fire-opening of capsule fruits. Fire neither promoted germination of hard-coated seeds nor affected their viability. Both the length of the seed-unproductive juvenile phase and post-fire habitat conditions were revealed as critical in determining the recovery of hard-seeded species after a low-intensity fire. Species with a short life cycle produced seeds in the year subsequent to the disturbance, ensuring a progressive moderate recovery from the few individuals which emerged after fire. This was particularly true in the southern exposure, where other seed-softening factors not exclusively related to fire may operate in a more intense way, compensating the null effect on germination of the low-fire intensity. For Cistus clusii, with a relatively long juvenile-phase span (3 years), low post-fire germination coincided with a long seed-unproductive period, which finally overlapped with a dramatic seed bank viability depletion (63 68%). In the northern exposure, fire resulted in a complete extinction of this species.
    Keywords forests ; habitats ; fruits ; juveniles ; seed germination ; seed dispersal ; Pinus halepensis ; ecological succession ; buried seeds ; Cistus ; viability ; extinction ; population dynamics ; soil
    Language English
    Document type Article
    ISSN 0792-9978
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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