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  1. Article ; Online: Lung type II alveolar epithelial cells collaborate with CCR2+ inflammatory monocytes in host defense against poxvirus infection

    Ning Yang / Joseph M. Luna / Peihong Dai / Yi Wang / Charles M. Rice / Liang Deng

    Nature Communications, Vol 13, Iss 1, Pp 1-

    2022  Volume 17

    Abstract: Smallpox is a highly contagious respiratory pathogen associated with a high mortality rate. Here the authors utilize a mouse model of intranasal vaccinia virus infection and show a C7 gene encoded virulence factor attenuates type I IFN release by lung ... ...

    Abstract Smallpox is a highly contagious respiratory pathogen associated with a high mortality rate. Here the authors utilize a mouse model of intranasal vaccinia virus infection and show a C7 gene encoded virulence factor attenuates type I IFN release by lung type II alveolar epithelial cells and reduces lung inflammatory monocyte responses.
    Keywords Science ; Q
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Global mapping of miRNA-target interactions in cattle (Bos taurus)

    Troels K. H. Scheel / Michael J. Moore / Joseph M. Luna / Eiko Nishiuchi / John Fak / Robert B. Darnell / Charles M. Rice

    Scientific Reports, Vol 7, Iss 1, Pp 1-

    2017  Volume 13

    Abstract: Abstract With roles in development, cell proliferation and disease, micro-RNA (miRNA) biology is of great importance and a potential therapeutic target. Here we used cross-linking immunoprecipitation (CLIP) and ligation of miRNA-target chimeras on the ... ...

    Abstract Abstract With roles in development, cell proliferation and disease, micro-RNA (miRNA) biology is of great importance and a potential therapeutic target. Here we used cross-linking immunoprecipitation (CLIP) and ligation of miRNA-target chimeras on the Argonaute (AGO) protein to globally map miRNA interactions in the cow. The interactome is the deepest reported to date. miRNA targeting principles are consistent with observations in other species, but with expanded pairing rules. Experimental mapping robustly predicted functional miR-17 regulatory sites. From miRNA-specific targeting for >5000 mRNAs we determined gene ontologies (GO). This confirmed repression of genes important for embryonic development and cell cycle progress by the let-7 family, and repression of those involved in cell cycle arrest by the miR-17 family, but also suggested a number of unappreciated miRNA functions. Our results provide a significant resource for understanding of bovine and species-conserved miRNA regulation, and demonstrate the power of experimental methods for establishing comprehensive interaction maps.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2017-08-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Correction

    Yingpu Yu / Troels K H Scheel / Joseph M Luna / Hachung Chung / Eiko Nishiuchi / Margaret A Scull / Natalia Echeverría / Inna Ricardo-Lax / Amit Kapoor / W Ian Lipkin / Thomas J Divers / Douglas F Antczak / Bud C Tennant / Charles M Rice

    PLoS Pathogens, Vol 14, Iss 9, p e

    miRNA independent hepacivirus variants suggest a strong evolutionary pressure to maintain miR-122 dependence.

    2018  Volume 1007303

    Abstract: This corrects the article DOI:10.1371/journal.ppat.1006694.]. ...

    Abstract [This corrects the article DOI:10.1371/journal.ppat.1006694.].
    Keywords Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2018-09-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: miRNA independent hepacivirus variants suggest a strong evolutionary pressure to maintain miR-122 dependence.

    Yingpu Yu / Troels K H Scheel / Joseph M Luna / Hachung Chung / Eiko Nishiuchi / Margaret A Scull / Natalia Echeverría / Inna Ricardo-Lax / Amit Kapoor / W Ian Lipkin / Thomas J Divers / Douglas F Antczak / Bud C Tennant / Charles M Rice

    PLoS Pathogens, Vol 13, Iss 10, p e

    2017  Volume 1006694

    Abstract: Hepatitis C virus (HCV) requires the liver specific micro-RNA (miRNA), miR-122, to replicate. This was considered unique among RNA viruses until recent discoveries of HCV-related hepaciviruses prompting the question of a more general miR-122 dependence. ... ...

    Abstract Hepatitis C virus (HCV) requires the liver specific micro-RNA (miRNA), miR-122, to replicate. This was considered unique among RNA viruses until recent discoveries of HCV-related hepaciviruses prompting the question of a more general miR-122 dependence. Among hepaciviruses, the closest known HCV relative is the equine non-primate hepacivirus (NPHV). Here, we used Argonaute cross-linking immunoprecipitation (AGO-CLIP) to confirm AGO binding to the single predicted miR-122 site in the NPHV 5'UTR in vivo. To study miR-122 requirements in the absence of NPHV-permissive cell culture systems, we generated infectious NPHV/HCV chimeric viruses with the 5' end of NPHV replacing orthologous HCV sequences. These chimeras were viable even in cells lacking miR-122, although miR-122 presence enhanced virus production. No other miRNAs bound this region. By random mutagenesis, we isolated HCV variants partially dependent on miR-122 as well as robustly replicating NPHV/HCV variants completely independent of any miRNAs. These miRNA independent variants even replicate and produce infectious particles in non-hepatic cells after exogenous delivery of apolipoprotein E (ApoE). Our findings suggest that miR-122 independent HCV and NPHV variants have arisen and been sampled during evolution, yet miR-122 dependence has prevailed. We propose that hepaciviruses may use this mechanism to guarantee liver tropism and exploit the tolerogenic liver environment to avoid clearance and promote chronicity.
    Keywords Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
    Subject code 500
    Language English
    Publishing date 2017-10-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.

    Pradeep D Uchil / Brian D Quinlan / Wai-Tsing Chan / Joseph M Luna / Walther Mothes

    PLoS Pathogens, Vol 4, Iss 2, p e

    2008  Volume 16

    Abstract: Members of the TRIpartite interaction Motif (TRIM) family of E3 ligases have been shown to exhibit antiviral activities. Here we report a near comprehensive screen for antiretroviral activities of 55 TRIM proteins (36 human, 19 mouse). We identified ... ...

    Abstract Members of the TRIpartite interaction Motif (TRIM) family of E3 ligases have been shown to exhibit antiviral activities. Here we report a near comprehensive screen for antiretroviral activities of 55 TRIM proteins (36 human, 19 mouse). We identified approximately 20 TRIM proteins that, when transiently expressed in HEK293 cells, affect the entry or release of human immunodeficiency virus 1 (HIV), murine leukemia virus (MLV), or avian leukosis virus (ALV). While TRIM11 and 31 inhibited HIV entry, TRIM11 enhanced N-MLV entry by interfering with Ref1 restriction. Strikingly, many TRIM proteins affected late stages of the viral life cycle. Gene silencing of endogenously expressed TRIM 25, 31, and 62 inhibited viral release indicating that they play an important role at late stages of the viral life cycle. In contrast, downregulation of TRIM11 and 15 enhanced virus release suggesting that these proteins contribute to the endogenous restriction of retroviruses in cells.
    Keywords Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2008-02-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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  6. Article ; Online: Male germ cells support long-term propagation of Zika virus

    Christopher L. Robinson / Angie C. N. Chong / Alison W. Ashbrook / Ginnie Jeng / Julia Jin / Haiqi Chen / Elizabeth I. Tang / Laura A. Martin / Rosa S. Kim / Reyn M. Kenyon / Eileen Do / Joseph M. Luna / Mohsan Saeed / Lori Zeltser / Harold Ralph / Vanessa L. Dudley / Marc Goldstein / Charles M. Rice / C. Yan Cheng /
    Marco Seandel / Shuibing Chen

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Volume 11

    Abstract: Zika virus (ZIKV) can persist for months in semen and sperm. Here, the authors show that germ cells, compared to other cell types in the reproductive tract, are most susceptible to ZIKV and produce high levels of progeny virus, which coincides with ... ...

    Abstract Zika virus (ZIKV) can persist for months in semen and sperm. Here, the authors show that germ cells, compared to other cell types in the reproductive tract, are most susceptible to ZIKV and produce high levels of progeny virus, which coincides with decreased expression of the interferon-stimulated gene Ifi44l.
    Keywords Science ; Q
    Language English
    Publishing date 2018-05-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Male germ cells support long-term propagation of Zika virus

    Christopher L. Robinson / Angie C. N. Chong / Alison W. Ashbrook / Ginnie Jeng / Julia Jin / Haiqi Chen / Elizabeth I. Tang / Laura A. Martin / Rosa S. Kim / Reyn M. Kenyon / Eileen Do / Joseph M. Luna / Mohsan Saeed / Lori Zeltser / Harold Ralph / Vanessa L. Dudley / Marc Goldstein / Charles M. Rice / C. Yan Cheng /
    Marco Seandel / Shuibing Chen

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Volume 11

    Abstract: Zika virus (ZIKV) can persist for months in semen and sperm. Here, the authors show that germ cells, compared to other cell types in the reproductive tract, are most susceptible to ZIKV and produce high levels of progeny virus, which coincides with ... ...

    Abstract Zika virus (ZIKV) can persist for months in semen and sperm. Here, the authors show that germ cells, compared to other cell types in the reproductive tract, are most susceptible to ZIKV and produce high levels of progeny virus, which coincides with decreased expression of the interferon-stimulated gene Ifi44l.
    Keywords Science ; Q
    Language English
    Publishing date 2018-05-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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  8. Article ; Online: DRUL for school

    Mayu O Frank / Nathalie E Blachere / Salina Parveen / Ezgi Hacisuleyman / John Fak / Joseph M Luna / Eleftherios Michailidis / Samara Wright / Pamela Stark / Ann Campbell / Ashley Foo / Thomas P Sakmar / Virginia Huffman / Marissa Bergh / Audrey Goldfarb / Andres Mansisidor / Agata L Patriotis / Karl H Palmquist / Nicolas Poulton /
    Rachel Leicher / César D M Vargas / Irene Duba / Arlene Hurley / Joseph Colagreco / Nicole Pagane / Dana E Orange / Kevin Mora / Jennifer L Rakeman / Randal C Fowler / Helen Fernandes / Michelle F Lamendola-Essel / Nicholas Didkovsky / Leopolda Silvera / Joseph Masci / Machelle Allen / Charles M Rice / Robert B Darnell

    PLoS ONE, Vol 16, Iss 6, p e

    Opening Pre-K with safe, simple, sensitive saliva testing for SARS-CoV-2.

    2021  Volume 0252949

    Abstract: To address the need for simple, safe, sensitive, and scalable SARS-CoV-2 tests, we validated and implemented a PCR test that uses a saliva collection kit use at home. Individuals self-collected 300 μl saliva in vials containing Darnell Rockefeller ... ...

    Abstract To address the need for simple, safe, sensitive, and scalable SARS-CoV-2 tests, we validated and implemented a PCR test that uses a saliva collection kit use at home. Individuals self-collected 300 μl saliva in vials containing Darnell Rockefeller University Laboratory (DRUL) buffer and extracted RNA was assayed by RT-PCR (the DRUL saliva assay). The limit of detection was confirmed to be 1 viral copy/μl in 20 of 20 replicate extractions. Viral RNA was stable in DRUL buffer at room temperature up to seven days after sample collection, and safety studies demonstrated that DRUL buffer immediately inactivated virus at concentrations up to 2.75x106 PFU/ml. Results from SARS-CoV-2 positive nasopharyngeal (NP) swab samples collected in viral transport media and assayed with a standard FDA Emergency Use Authorization (EUA) test were highly correlated with samples placed in DRUL buffer. Direct comparison of results from 162 individuals tested by FDA EUA oropharyngeal (OP) or NP swabs with co-collected saliva samples identified four otherwise unidentified positive cases in DRUL buffer. Over six months, we collected 3,724 samples from individuals ranging from 3 months to 92 years of age. This included collecting weekly samples over 10 weeks from teachers, children, and parents from a pre-school program, which allowed its safe reopening while at-risk pods were quarantined. In sum, we validated a simple, sensitive, stable, and safe PCR-based test using a self-collected saliva sample as a valuable tool for clinical diagnosis and screening at workplaces and schools.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Human TRIM gene expression in response to interferons.

    Laetitia Carthagena / Anna Bergamaschi / Joseph M Luna / Annie David / Pradeep D Uchil / Florence Margottin-Goguet / Walther Mothes / Uriel Hazan / Catherine Transy / Gianfranco Pancino / Sébastien Nisole

    PLoS ONE, Vol 4, Iss 3, p e

    2009  Volume 4894

    Abstract: Background Tripartite motif (TRIM) proteins constitute a family of proteins that share a conserved tripartite architecture. The recent discovery of the anti-HIV activity of TRIM5alpha in primate cells has stimulated much interest in the potential role of ...

    Abstract Background Tripartite motif (TRIM) proteins constitute a family of proteins that share a conserved tripartite architecture. The recent discovery of the anti-HIV activity of TRIM5alpha in primate cells has stimulated much interest in the potential role of TRIM proteins in antiviral activities and innate immunity. Principal findings To test if TRIM genes are up-regulated during antiviral immune responses, we performed a systematic analysis of TRIM gene expression in human primary lymphocytes and monocyte-derived macrophages in response to interferons (IFNs, type I and II) or following FcgammaR-mediated activation of macrophages. We found that 27 of the 72 human TRIM genes are sensitive to IFN. Our analysis identifies 9 additional TRIM genes that are up-regulated by IFNs, among which only 3 have previously been found to display an antiviral activity. Also, we found 2 TRIM proteins, TRIM9 and 54, to be specifically up-regulated in FcgammaR-activated macrophages. Conclusions Our results present the first comprehensive TRIM gene expression analysis in primary human immune cells, and suggest the involvement of additional TRIM proteins in regulating host antiviral activities.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2009-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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