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  1. Article ; Online: Journal clubs in the time of preprints

    Prachee Avasthi / Alice Soragni / Joshua N Bembenek

    eLife, Vol

    2018  Volume 7

    Abstract: Early-career researchers can learn about peer review by discussing preprints at journal clubs and sending feedback to the authors. ...

    Abstract Early-career researchers can learn about peer review by discussing preprints at journal clubs and sending feedback to the authors.
    Keywords peer review ; preprints ; early-career researchers ; journal clubs ; open science ; graduate training ; Medicine ; R ; Science ; Q ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2018-06-01T00:00:00Z
    Publisher eLife Sciences Publications Ltd
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Conservation of the separase regulatory domain

    Michael Melesse / Joshua N. Bembenek / Igor B. Zhulin

    Biology Direct, Vol 13, Iss 1, Pp 1-

    2018  Volume 7

    Abstract: Abstract ᅟ We report a protein sequence analysis of the cell cycle regulatory protease, separase. The sequence and structural conservation of the C-terminal protease domain has long been recognized, whereas the N-terminal regulatory domain of separase ... ...

    Abstract Abstract ᅟ We report a protein sequence analysis of the cell cycle regulatory protease, separase. The sequence and structural conservation of the C-terminal protease domain has long been recognized, whereas the N-terminal regulatory domain of separase was reported to lack detectable sequence similarity. Here we reveal significant sequence conservation of the separase regulatory domain and report a discovery of a cysteine motif (CxCxxC) conserved in major lineages of Metazoa including nematodes and vertebrates. This motif is found in a solvent exposed linker region connecting two TPR-like helical motifs. Mutation of this motif in Caenorhabditis elegans separase leads to a temperature sensitive hypomorphic protein. Conservation of this motif in organisms ranging from C. elegans to humans suggests its functional importance. Reviewers This article was reviewed by Lakshminarayan Iyer and Michael Galperin.
    Keywords Separase ; Conservation ; PSI-BLAST ; Cysteine motif ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2018-04-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Protease-dead separase is dominant negative in the C. elegans embryo.

    Diana M Mitchell / Lindsey R Uehlein-Klebanow / Joshua N Bembenek

    PLoS ONE, Vol 9, Iss 9, p e

    2014  Volume 108188

    Abstract: Separase is a protease that promotes chromosome segregation at anaphase by cleaving cohesin. Several non-proteolytic functions of separase have been identified in other organisms. We created a transgenic C. elegans line that expresses protease-dead ... ...

    Abstract Separase is a protease that promotes chromosome segregation at anaphase by cleaving cohesin. Several non-proteolytic functions of separase have been identified in other organisms. We created a transgenic C. elegans line that expresses protease-dead separase in embryos to further characterize separase function. We find that expression of protease-dead separase is dominant-negative in C. elegans embryos, not previously reported in other systems. The C. elegans embryo is an ideal system to study developmental processes in a genetically tractable system. However, a major limitation is the lack of an inducible gene expression system for the embryo. We have developed two methods that allow for the propagation of lines carrying dominant-negative transgenes and have applied them to characterize expression of protease-dead separase in embryos. Using these methods, we show that protease-dead separase causes embryo lethality, and that protease-dead separase cannot rescue separase mutants. These data suggest that protease-dead separase interferes with endogenous separase function, possibly by binding substrates and protecting them from cleavage.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: BUB-1 targets PP2A:B56 to regulate chromosome congression during meiosis I in C. elegans oocytes

    Laura Bel Borja / Flavie Soubigou / Samuel J P Taylor / Conchita Fraguas Bringas / Jacqueline Budrewicz / Pablo Lara-Gonzalez / Christopher G Sorensen Turpin / Joshua N Bembenek / Dhanya K Cheerambathur / Federico Pelisch

    eLife, Vol

    2020  Volume 9

    Abstract: Protein Phosphatase 2A (PP2A) is a heterotrimer composed of scaffolding (A), catalytic (C), and regulatory (B) subunits. PP2A complexes with B56 subunits are targeted by Shugoshin and BUBR1 to protect centromeric cohesion and stabilise kinetochore– ... ...

    Abstract Protein Phosphatase 2A (PP2A) is a heterotrimer composed of scaffolding (A), catalytic (C), and regulatory (B) subunits. PP2A complexes with B56 subunits are targeted by Shugoshin and BUBR1 to protect centromeric cohesion and stabilise kinetochore–microtubule attachments in yeast and mouse meiosis. In Caenorhabditis elegans, the closest BUBR1 orthologue lacks the B56-interaction domain and Shugoshin is not required for meiotic segregation. Therefore, the role of PP2A in C. elegans female meiosis is unknown. We report that PP2A is essential for meiotic spindle assembly and chromosome dynamics during C. elegans female meiosis. BUB-1 is the main chromosome-targeting factor for B56 subunits during prometaphase I. BUB-1 recruits PP2A:B56 to the chromosomes via a newly identified LxxIxE motif in a phosphorylation-dependent manner, and this recruitment is important for proper chromosome congression. Our results highlight a novel mechanism for B56 recruitment, essential for recruiting a pool of PP2A involved in chromosome congression during meiosis I.
    Keywords PP2A ; meiosis ; Bub1 ; B56 ; SLiM ; Medicine ; R ; Science ; Q ; Biology (General) ; QH301-705.5
    Subject code 571
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher eLife Sciences Publications Ltd
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

    Chen, Bi-Chang / Alex T. Ritter / Anne-Cecile Reymann / Brian P. English / Chris Janetopoulos / Daniel E. Milkie / Daniel P. Kiehart / Daniel P. Romero / Diana M. Mitchell / Eric Betzig / Geraldine Seydoux / Jennifer Lippincott-Schwartz / Jennifer T. Wang / John A. Hammer / Joshua N. Bembenek / Kai Wang / Lillian Fritz-Laylin / Lin Shao / Michael W. Davidson /
    R. Dyche Mullins / Ralph Böhme / Stephan W. Grill / U. Serdar Tulu / Wesley R. Legant / Xufeng S. Wu / Yuko Mimori-Kiyosue / Zhe Liu

    Science. 2014 Oct. 24, v. 346, no. 6208

    2014  

    Abstract: From single molecules to embryos in living color Animation defines life, and the three-dimensional (3D) imaging of dynamic biological processes occurring within living specimens is essential to understand life. However, in vivo imaging, especially in 3D, ...

    Abstract From single molecules to embryos in living color Animation defines life, and the three-dimensional (3D) imaging of dynamic biological processes occurring within living specimens is essential to understand life. However, in vivo imaging, especially in 3D, involves inevitable tradeoffs of resolution, speed, and phototoxicity. Chen et al. describe a microscope that can address these concerns. They used a class of nondiffracting beams, known as 2D optical lattices, which spread the excitation energy across the entire field of view while simultaneously eliminating out-of-focus excitation. Lattice light sheets increase the speed of image acquisition and reduce phototoxicity, which expands the range of biological problems that can be investigated. The authors illustrate the power of their approach using 20 distinct biological systems ranging from single-molecule binding kinetics to cell migration and division, immunology, and embryonic development. Science , this issue 10.1126/science.1257998
    Keywords cell movement ; color ; embryogenesis ; energy ; image analysis ; immunology ; light ; microscopy ; phototoxicity
    Language English
    Dates of publication 2014-1024
    Size p. 1257998.
    Publishing place American Association for the Advancement of Science
    Document type Article
    ZDB-ID 128410-1
    ISSN 1095-9203 ; 0036-8075
    ISSN (online) 1095-9203
    ISSN 0036-8075
    DOI 10.1126/science.1257998
    Database NAL-Catalogue (AGRICOLA)

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