Article ; Online: Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic Application.
2023 Volume 13, Issue 2
Abstract: With the move of molecular tests from diagnostic labs to on-site testing becoming more common, there is a sudden rise in demand for nucleic acid-based diagnostic tools that are selective, sensitive, flexible to terrain changes, and cost-effective to ... ...
| Abstract | With the move of molecular tests from diagnostic labs to on-site testing becoming more common, there is a sudden rise in demand for nucleic acid-based diagnostic tools that are selective, sensitive, flexible to terrain changes, and cost-effective to assist in point-of-care systems for large-scale screening and to be used in remote locations in cases of outbreaks and pandemics. CRISPR-based biosensors comprise a promising new approach to nucleic acid detection, which uses Cas effector proteins (Cas9, Cas12, and Cas13) as extremely specialized identification components that may be used in conjunction with a variety of readout approaches (such as fluorescence, colorimetry, potentiometry, lateral flow assay, etc.) for onsite analysis. In this review, we cover some technical aspects of integrating the CRISPR Cas system with traditional biosensing readout methods and amplification technologies such as polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), and recombinase polymerase amplification (RPA) and continue to elaborate on the prospects of the developed biosensor in the detection of some major viral and bacterial diseases. Within the scope of this article, we also discuss the recent COVID pandemic and the numerous CRISPR biosensors that have undergone development since its advent. Finally, we discuss some challenges and future prospects of CRISPR Cas systems in point-of-care testing. |
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| MeSH term(s) | Humans ; Point-of-Care Systems ; COVID-19 ; Point-of-Care Testing ; Biological Assay ; Biosensing Techniques ; Nucleic Acids ; Nucleic Acid Amplification Techniques ; COVID-19 Testing |
| Chemical Substances | Nucleic Acids |
| Language | English |
| Publishing date | 2023-01-29 |
| Publishing country | Switzerland |
| Document type | Journal Article ; Review |
| ZDB-ID | 2662125-3 |
| ISSN | 2079-6374 ; 2079-6374 |
| ISSN (online) | 2079-6374 |
| ISSN | 2079-6374 |
| DOI | 10.3390/bios13020202 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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