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Book ; Online: The Figurativeness of the Language of Mystical Experience

Bakešová, Václava / Brezováková, Monika / Brodňanová, Silvia / Campana, Silvia / Civáňová, Zuzana / Fermín, Javier / Gallik, Ján / Gritti, Fabiano / Horka, Róbert / Hornáčková Klapicová, Edita / Juhásová, Jana / Kaizerová, Petra / Knapík, Ján / Mancebi, Juan / Pariláková, Eva / Príhodová, Edita / Rathouzská, Lucie / Rybárová, Silvia / Šavelová, Monika /

Štěpánek, Pavel / Tierraseca, Mónica / Raušerová, Andrea / Sancho Fermín, Francisco / Barnés, Antonio / Kučerková, Magda

Particularities and Interpretations

2021

Keywords	Literary studies: general ; mystical experience, individual and spontaneous experience, tradition, imagery, literary form, mystery, art
Language	0\|e
Size	1 electronic resource (278 pages)
Publisher	Masaryk University Press
Document type	Book ; Online
Note	English ; Open Access
HBZ-ID	HT021610904
ISBN	9788021099975 ; 8021099976
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Surface Microdialysis for Detection of Colorectal Anastomosis Ischemia-An Experimental Study.

Ryska, Ondrej / Kalvach, Jaroslav / Pazin, Jaroslav / Hadac, Jan / Martinek, Jan / Juhas, Stefan / Juhasova, Jana

The Journal of surgical research

2021 Volume 261, Page(s) 293–300

Abstract: Background: Inadequate blood supply is one of the major risk factors for anastomotic leak after low anterior rectal resection. Early detection of ischemia might predict complicated healing and enable anastomotic salvage, which is associated with better ... ...

Abstract	Background: Inadequate blood supply is one of the major risk factors for anastomotic leak after low anterior rectal resection. Early detection of ischemia might predict complicated healing and enable anastomotic salvage, which is associated with better outcomes. A microdialysis offers a real-time evaluation of adequate bowel perfusion through monitoring of tissue metabolism. In this experimental study, we assessed the role of microdialysis in detecting early ischemia after colorectal anastomosis. Materials and methods: Colorectal anastomosis was performed in six miniature pigs. A microdialysis catheter was placed on the bowel serosa 5 mm proximal to the anastomosis. Metabolic changes were monitored hourly before and after initiating ischemia, which was induced by ligation of the inferior mesenteric artery and skeletonization of the mesocolon. Results: Significant increase in tissue levels of lactate was detected after 60 min of ischemia (13.6 [10.4-16.1] versus 6.75 [1.8-9.6] mmol/L at baseline; P < 0.005). The lactate/pyruvate ratio increased accordingly. The concentration of glycerol increased significantly after 2 hours-from a baseline value of 29.5 (3-84) to 125 (79-201) mmol/L (P < 0.005). The decrease in glucose levels was also significant after 60 minutes-0 (0-0.2) versus 4.7 (3.3-6.8) mmol/L at baseline. However, its values started to decline before ischemia. Conclusions: Surface microdialysis can detect ischemic changes early and may be a promising method in postoperative monitoring of colorectal anastomosis.
MeSH term(s)	Anastomosis, Surgical/adverse effects ; Animals ; Blood Glucose/analysis ; Female ; Glycerol/metabolism ; Ischemia/diagnosis ; Ischemia/etiology ; Ischemia/metabolism ; Lactic Acid/metabolism ; Male ; Microdialysis/methods ; Postoperative Complications/diagnosis ; Postoperative Complications/etiology ; Postoperative Complications/metabolism ; Swine ; Swine, Miniature
Chemical Substances	Blood Glucose ; Lactic Acid (33X04XA5AT) ; Glycerol (PDC6A3C0OX)
Language	English
Publishing date	2021-01-18
Publishing country	United States
Document type	Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80170-7
ISSN	1095-8673 ; 0022-4804
ISSN (online)	1095-8673
ISSN	0022-4804
DOI	10.1016/j.jss.2020.12.021
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Zs.A 178: Show issues

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Article ; Online: Impact factors on cervical dilation rates in the first stage of labor.

Juhasova, Jana / Kreft, Martina / Zimmermann, Roland / Kimmich, Nina

Journal of perinatal medicine

2017 Volume 46, Issue 1, Page(s) 59–66

Abstract: Aims: To assess cervical dilation rates of nulliparous and multiparous women in the active first stage of labor and to evaluate significant impact factors.: Methods: In a retrospective cohort study between January 2007 and July 2014 at the University ...

Abstract	Aims: To assess cervical dilation rates of nulliparous and multiparous women in the active first stage of labor and to evaluate significant impact factors. Methods: In a retrospective cohort study between January 2007 and July 2014 at the University Hospital of Zurich in Switzerland, we analyzed 8378 women with singleton pregnancies in vertex presentation with a vaginal delivery at 34+0 to 42+5 gestational weeks. Median cervical dilation rates were calculated and different impact factors evaluated. Results: Cervical dilation rates increase during labor progress with faster rates in multiparous compared with nulliparous women (P<0.001). Dilation rates exceed 1 cm/h at a dilatation of 6-7 cm, but are very individual. Accelerating impact factors are multiparity, a greater amount of cervical dilation and fetal occipitoanterior position, whereas the use of epidural anesthesia, a higher fetal weight and head circumference decelerate dilation (P<0.001). Conclusion: Cervical dilation is a hyperbolic increasing process, with faster dilation rates in multiparous compared to nulliparous women and a reversal point of labor around 6-7 cm, respectively. Besides, cervical dilation is highly individual and affected by several impact factors. The diagnosis of labor arrest or prolonged labor should therefore be based on such rates and on the individual evaluation of every woman.
MeSH term(s)	Adult ; Cervix Uteri/physiology ; Female ; Humans ; Labor Stage, First ; Parity ; Pregnancy ; Retrospective Studies ; Young Adult
Language	English
Publishing date	2017-06-29
Publishing country	Germany
Document type	Journal Article
ZDB-ID	123512-6
ISSN	1619-3997 ; 0300-5577 ; 0936-174X
ISSN (online)	1619-3997
ISSN	0300-5577 ; 0936-174X
DOI	10.1515/jpm-2016-0284
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Article ; Online: Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors:

Shigyo, Michiko / Kobayashi, Yoshiomi / Platoshyn, Oleksandr / Marsala, Silvia / Kato, Tomohisa / Takamura, Naoki / Yoshida, Kenji / Kishino, Akiyoshi / Bravo-Hernandez, Mariana / Juhas, Stefan / Juhasova, Jana / Studenovska, Hana / Proks, Vladimir / Ciacci, Joseph D / Marsala, Martin

Cell transplantation

2023 Volume 32, Page(s) 9636897231163232

Abstract: The critical requirements in developing clinical-grade human-induced pluripotent stem cells-derived neural precursors (hiPSCs-NPCs) are defined by expandability, genetic stability, ... ...

Abstract	The critical requirements in developing clinical-grade human-induced pluripotent stem cells-derived neural precursors (hiPSCs-NPCs) are defined by expandability, genetic stability, predictable
MeSH term(s)	Humans ; Rats ; Animals ; Induced Pluripotent Stem Cells ; Neural Stem Cells ; Sendai virus/genetics ; Leukocytes, Mononuclear ; Neurons/metabolism ; Cell Differentiation
Language	English
Publishing date	2023-04-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1135816-6
ISSN	1555-3892 ; 0963-6897
ISSN (online)	1555-3892
ISSN	0963-6897
DOI	10.1177/09636897231163232
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Article ; Online: Nanofiber Wound Dressing Materials-A Comparative Study of Wound Healing on a Porcine Model.

Menclová, Katerina / Svoboda, Petr / Hadač, Jan / Juhás, Štefan / Juhásová, Jana / Pejchal, Jaroslav / Mandys, Václav / Eminger, Karel / Ryska, Miroslav

Military medicine

2021 Volume 188, Issue 1-2, Page(s) e133–e139

Abstract: Background: Nanofiber wound dressings remain the domain of in vitro studies. The purpose of our study was to verify the benefits of chitosan (CTS) and polylactide (PLA)-based nanofiber wound dressings on a porcine model of a naturally contaminated ... ...

Abstract	Background: Nanofiber wound dressings remain the domain of in vitro studies. The purpose of our study was to verify the benefits of chitosan (CTS) and polylactide (PLA)-based nanofiber wound dressings on a porcine model of a naturally contaminated standardized wound and compare them with the conventional dressings, i.e., gauze and Inadine. Material and methods: The study group included 32 pigs randomized into four homogeneous groups according to the wound dressing type. Standardized wounds were created on their backs, and wound dressings were regularly changed. We evaluated difficulty of handling individual dressing materials and macroscopic appearance of the wounds. Wound swabs were taken for bacteriological examination. Blood samples were obtained to determine blood count values and serum levels of acute phase proteins (serum amyloid A, C-reactive protein, and haptoglobin). The crucial point of the study was histological analysis. Microscopic evaluation was focused on the defect depth and tissue reactions, including formation of the fibrin exudate with neutrophil granulocytes, the layer of granulation and cellular connective tissue, and the reepithelialization. Statistical analysis was performed by using SPSS software. The analysis was based on the Kruskal-Wallis H test and Mann-Whitney U test followed by Bonferroni correction. Significance was set at P < .05. Results: Macroscopic examination did not show any difference in wound healing among the groups. However, evaluation of histological findings demonstrated that PLA-based nanofiber dressing accelerated the proliferative (P = .025) and reepithelialization (P < .001) healing phases, while chitosan-based nanofiber dressing potentiated and accelerated the inflammatory phase (P = .006). No statistically significant changes were observed in the blood count or acute inflammatory phase proteins during the trial. Different dynamics were noted in serum amyloid A values in the group treated with PLA-based nanofiber dressing (P = .006). Conclusion: Based on the microscopic examination, we have documented a positive effect of nanofiber wound dressings on acceleration of individual phases of the healing process. Nanofiber wound dressings have a potential to become in future part of the common wound care practice.
MeSH term(s)	Animals ; Swine ; Nanofibers/therapeutic use ; Chitosan/pharmacology ; Chitosan/therapeutic use ; Serum Amyloid A Protein/pharmacology ; Wound Healing ; Bandages
Chemical Substances	Chitosan (9012-76-4) ; Serum Amyloid A Protein
Language	English
Publishing date	2021-05-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	391061-1
ISSN	1930-613X ; 0026-4075
ISSN (online)	1930-613X
ISSN	0026-4075
DOI	10.1093/milmed/usab155
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Article ; Online: Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors:

Kobayashi, Yoshiomi / Shigyo, Michiko / Platoshyn, Oleksandr / Marsala, Silvia / Kato, Tomohisa / Takamura, Naoki / Yoshida, Kenji / Kishino, Akiyoshi / Bravo-Hernandez, Mariana / Juhas, Stefan / Juhasova, Jana / Studenovska, Hana / Proks, Vladimir / Driscoll, Shawn P / Glenn, Thomas D / Pfaff, Samuel L / Ciacci, Joseph D / Marsala, Martin

Cell transplantation

2023 Volume 32, Page(s) 9636897221107009

Abstract: One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, ...

Abstract	One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that are simple to use is critical for potential future dissemination of any spinally targeted cell-replacement therapy into general medical practice. Here, we compared the engraftment properties of established human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cell (NPCs) line once cells were harvested fresh from the cell culture or previously frozen and then grafted into striata or spinal cord of the immunodeficient rat. A newly developed human spinal injection device equipped with a spinal cord pulsation-cancelation magnetic needle was also tested for its safety in an adult immunosuppressed pig. Previously frozen NPCs showed similar post-grafting survival and differentiation profile as was seen for freshly harvested cells. Testing of human injection device showed acceptable safety with no detectable surgical procedure or spinal NPCs injection-related side effects.
MeSH term(s)	Adult ; Animals ; Humans ; Rats ; Cell Differentiation/physiology ; Cellular Reprogramming/genetics ; Cellular Reprogramming/physiology ; Genetic Vectors/genetics ; Graft Survival/physiology ; Induced Pluripotent Stem Cells/physiology ; Induced Pluripotent Stem Cells/transplantation ; Injections, Spinal/adverse effects ; Injections, Spinal/instrumentation ; Injections, Spinal/methods ; Neural Stem Cells/physiology ; Neural Stem Cells/transplantation ; Sendai virus ; Specimen Handling/methods ; Stem Cell Transplantation/adverse effects ; Stem Cell Transplantation/instrumentation ; Stem Cell Transplantation/methods ; Swine ; Tissue and Organ Harvesting/methods ; Treatment Outcome ; Brain ; Spinal Cord
Language	English
Publishing date	2023-05-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1135816-6
ISSN	1555-3892 ; 0963-6897
ISSN (online)	1555-3892
ISSN	0963-6897
DOI	10.1177/09636897221107009
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Article ; Online: Huntingtin Co-Isolates with Small Extracellular Vesicles from Blood Plasma of TgHD and KI-HD Pig Models of Huntington's Disease and Human Blood Plasma.

Ananbeh, Hanadi / Novak, Jaromir / Juhas, Stefan / Juhasova, Jana / Klempir, Jiri / Doleckova, Kristyna / Rysankova, Irena / Turnovcova, Karolina / Hanus, Jaroslav / Hansikova, Hana / Vodicka, Petr / Kupcova Skalnikova, Helena

International journal of molecular sciences

2022 Volume 23, Issue 10

Abstract: 1) Background: Huntington's disease (HD) is rare incurable hereditary neurodegenerative disorder caused by CAG repeat expansion in the gene coding for the protein huntingtin (HTT). Mutated huntingtin (mHTT) undergoes fragmentation and accumulation, ... ...

Abstract	(1) Background: Huntington's disease (HD) is rare incurable hereditary neurodegenerative disorder caused by CAG repeat expansion in the gene coding for the protein huntingtin (HTT). Mutated huntingtin (mHTT) undergoes fragmentation and accumulation, affecting cellular functions and leading to neuronal cell death. Porcine models of HD are used in preclinical testing of currently emerging disease modifying therapies. Such therapies are aimed at reducing mHTT expression, postpone the disease onset, slow down the progression, and point out the need of biomarkers to monitor disease development and therapy efficacy. Recently, extracellular vesicles (EVs), particularly exosomes, gained attention as possible carriers of disease biomarkers. We aimed to characterize HTT and mHTT forms/fragments in blood plasma derived EVs in transgenic (TgHD) and knock-in (KI-HD) porcine models, as well as in HD patients' plasma. (2) Methods: Small EVs were isolated by ultracentrifugation and HTT forms were visualized by western blotting. (3) Results: The full length 360 kDa HTT co-isolated with EVs from both the pig model and HD patient plasma. In addition, a ~70 kDa mutant HTT fragment was specific for TgHD pigs. Elevated total huntingtin levels in EVs from plasma of HD groups compared to controls were observed in both pig models and HD patients, however only in TgHD were they significant (
MeSH term(s)	Animals ; Biomarkers ; Extracellular Vesicles/metabolism ; Humans ; Huntington Disease/metabolism ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; Plasma/metabolism ; Swine
Chemical Substances	Biomarkers ; Nerve Tissue Proteins
Language	English
Publishing date	2022-05-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23105598
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Article: Stenting to prevent esophageal stricture after circumferential endoscopic submucosal dissection: an experimental study.

Martinek, Jan / Dolezel, Radek / Walterova, Bara / Kollar, Marek / Juhas, Stefan / Juhasova, Jana / Vackova, Zuzana / Hustak, Rastislav / Erben, Jakub

Endoscopy international open

2020 Volume 8, Issue 11, Page(s) E1698–E1706

Abstract: Background and study ... ...

Abstract	Background and study aims
Language	English
Publishing date	2020-10-22
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2761052-4
ISSN	2196-9736 ; 2364-3722
ISSN (online)	2196-9736
ISSN	2364-3722
DOI	10.1055/a-1261-3103
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Article ; Online: A novel postgraduate endoscopic course using a large animal model of secondary Crohn's disease stricture.

Lukas, Martin / Kolar, Martin / Ryska, Ondrej / Juhas, Stefan / Juhasova, Jana / Kalvach, Jaroslav / Pazin, Jaroslav / Kocisova, Tereza / Foltan, Ondrej / Kristianova, Hana / Ptacnik, Jan / Vitkova, Ivana / Bortlik, Martin / Lukas, Milan

Surgical endoscopy

2021 Volume 35, Issue 6, Page(s) 3199–3204

Abstract: Background: The increasing complexity of advanced endoscopic techniques places a high demand on the endoscopist's expertise. Thus, live porcine models have been more frequently used for training. We briefly describe a hands-on postgraduate endoscopic ... ...

Abstract	Background: The increasing complexity of advanced endoscopic techniques places a high demand on the endoscopist's expertise. Thus, live porcine models have been more frequently used for training. We briefly describe a hands-on postgraduate endoscopic course regarding a novel method of treatment of anastomotic strictures in a porcine model. Methods: The porcine model of Crohn's disease anastomotic stricture with two artificial side-to-side ileo-colonic anastomoses was used. Participants performed endoscopic stricturotomy under supervision at one of two equipped endoscopic stations. Available animals were endoscopically re-examined 3 months after the course. Results: Twelve anastomoses were prepared for the course. Eleven circumferential stricturotomies together with horizontal cut and clip placement were conducted. All anastomoses were passable for the scope after the procedure, and no case of perforation or bleeding occurred. All anastomoses available for re-examination remained passable for the endoscope after 3 months. Conclusion: We successfully organised the first endoscopic hands-on course for the training of endoscopic stricturotomy on a large animal model.
MeSH term(s)	Animals ; Constriction, Pathologic/etiology ; Constriction, Pathologic/surgery ; Crohn Disease/complications ; Crohn Disease/surgery ; Dilatation ; Disease Models, Animal ; Humans ; Swine ; Treatment Outcome
Language	English
Publishing date	2021-03-04
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639039-0
ISSN	1432-2218 ; 0930-2794
ISSN (online)	1432-2218
ISSN	0930-2794
DOI	10.1007/s00464-021-08360-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 2214: Show issues

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Article: Isolation and Characterization of Small Extracellular Vesicles from Porcine Blood Plasma, Cerebrospinal Fluid, and Seminal Plasma.

Skalnikova, Helena Kupcova / Bohuslavova, Bozena / Turnovcova, Karolina / Juhasova, Jana / Juhas, Stefan / Rodinova, Marie / Vodicka, Petr

Proteomes

2019 Volume 7, Issue 2

Abstract: Extracellular vesicles (EVs) are a highly attractive subject of biomedical research as possible carriers of nucleic acid and protein biomarkers. EVs released to body fluids enable indirect access to inner organs by so-called "liquid biopsies". Obtaining ... ...

Abstract	Extracellular vesicles (EVs) are a highly attractive subject of biomedical research as possible carriers of nucleic acid and protein biomarkers. EVs released to body fluids enable indirect access to inner organs by so-called "liquid biopsies". Obtaining a high-quality EV sample with minimum contaminants is crucial for proteomic analyses using LC-MS/MS or other techniques. However, the EV content in various body fluids largely differs, which may hamper subsequent analyses. Here, we present a comparison of extracellular vesicle yields from blood plasma, cerebrospinal fluid, and seminal plasma using an experimental pig model. Pigs are widely used in biomedical research as large animal models with anatomy and physiology close to those of humans and enable studies (e.g., of the nervous system) that are unfeasible in humans. EVs were isolated from body fluids by differential centrifugation followed by ultracentrifugation. EVs were characterized according to protein yields and to the quality of the isolated vesicles (e.g., size distribution, morphology, positivity for exosome markers). In our experimental setting, substantial differences in EV amounts were identified among body fluids, with the seminal plasma being the richest EV source. The yields of pellet proteins from ultracentrifugation of 1 mL of porcine body fluids may help to estimate body fluid input volumes to obtain sufficient samples for subsequent proteomic analyses.
Language	English
Publishing date	2019-04-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720995-7
ISSN	2227-7382
ISSN	2227-7382
DOI	10.3390/proteomes7020017
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