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  1. Article ; Online: Decisional conflict in American parents regarding newborn circumcision.

    Botkin, Hannah / Juhr, Denise / Storm, Douglas W / Cooper, Christopher S / Edwards, Angelena / Lockwood, Gina M

    Journal of pediatric urology

    2023  Volume 19, Issue 5, Page(s) 608–618

    Abstract: Background: Decisional conflict surrounding the topic of circumcision in the newborn male is assumed in some parents but has not been quantified or qualified. It is known that parents often base their decision on cultural and social factors and that ... ...

    Abstract Background: Decisional conflict surrounding the topic of circumcision in the newborn male is assumed in some parents but has not been quantified or qualified. It is known that parents often base their decision on cultural and social factors and that physician discussions do affect ultimate decision-making. Information on parents' decision-making surrounding newborn circumcision and ways to mitigate conflict or uncertainty around the decision-making process is needed to better counsel them appropriately.
    Objectives: To identify the presence or absence of decisional conflict in parents-to-be deciding whether or not to circumcise their child as well as to identify determinants of this conflict to direct future educational measures.
    Study design: Parents presenting to obstetrics clinic as well as contacted by institutional email were recruited using convenience sampling and completed the validated Decisional Conflict Scale (DCS). A smaller subset of subjects were recruited via institutional email to complete semi-structured interviews regarding the decision-making process and specifically uncertainty regarding the decision. Descriptive statistics and unpaired t tests were used for analysis of survey data. For interview data, an iterative, grounded theory methodology was used.
    Results: 173 subjects completed the DCS. 12% of all participants had high decisional conflict. Intuitively, those who had not yet decided whether to circumcise had the highest proportion of high DCS (69%), followed by those who had decided to circumcise (9.3%) and those who had decided not to circumcise (1.7%). 24 subjects were interviewed, and based on their DCS scores and interview responses were classified as low, intermediate and high conflict. Three primary themes emerged delineating the high from low conflict groups. There were notable differences in the feelings of subjects regarding knowledge and feeling informed, the importance of particular values and clarity of the roles of these values in decision-making, and feelings of supported decision-making. These themes were used to create a visual model depicting the individual needs of each decision-maker (Fig. 1).
    Discussion: This study highlights the need for decision support for parents that is not only information-based but focuses on values clarity and supported decision-making. This study provides a jumping-off point for creation of shared decision-making tools directed at individual needs. The limitations of this study are a single institution design and homogeneous population, so when designing materials, additional unrecognized needs will likely be identified.
    Conclusion: A small, but real proportion of parents-to-be experience significant uncertainty around the decision to circumcise their newborn boys. Identified needs of parents include feeling informed, feeling supported and clarification of important values related to the problem.
    MeSH term(s) Humans ; Infant, Newborn ; Male ; Circumcision, Male ; Decision Making ; Emotions ; Parents ; Surveys and Questionnaires ; United States
    Language English
    Publishing date 2023-05-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 2237683-5
    ISSN 1873-4898 ; 1477-5131
    ISSN (online) 1873-4898
    ISSN 1477-5131
    DOI 10.1016/j.jpurol.2023.05.015
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  2. Article ; Online: A Child's urine is not sterile: A pilot study evaluating the Pediatric Urinary Microbiome.

    Storm, Douglas W / Copp, Hillary L / Halverson, Thomas M / Du, Jingjie / Juhr, Denise / Wolfe, Alan J

    Journal of pediatric urology

    2022  Volume 18, Issue 3, Page(s) 383–392

    Abstract: Introduction: A bladder microbiome (urobiome) exists in adults. Data supports the effects of the adult urobiome on urinary tract health with associations between dysbiotic urobiomes and lower urinary tract disorders. Understanding urobiome origin is ... ...

    Abstract Introduction: A bladder microbiome (urobiome) exists in adults. Data supports the effects of the adult urobiome on urinary tract health with associations between dysbiotic urobiomes and lower urinary tract disorders. Understanding urobiome origin is important since other microbiomes establish around birth and microbiome alterations are linked to disease development. However, the pediatric urobiome has not been well studied.
    Objectives: We sought to determine the age when the urobiome develops, compare the pediatric urobiome to microbiomes of adjacent urogenital niches, and compare the urobiomes between boys and girls and across age groups.
    Study design: Seventy-four children less than 18 years of age without recent antibiotic exposure were recruited, including 48 males and 26 females, aged 2 weeks to 209 months of age. Transurethral catheterized urine samples and samples from the perineum, urethra, vagina, and foreskin were collected. Specimens were assessed using the expanded quantitative urine culture protocol and by 16S rRNA gene sequencing. Dada2 was used to profile microbial compositions, and BLCA was used to identify microbial taxa.
    Results: Bacteria were detected in 90.5% of urine samples and identified in children as young as 2 weeks of age. Microbial communities and compositions of the female bladder and other urogenital niches (urethra, perineum, and vagina) differed significantly by age. Lactobacillus predominated the bladder, urethral, and vaginal microbiomes in post-pubertal girls. Compared to female urinary microbiomes, those of males differed less substantially. Only perineal microbiomes differed significantly by age, whereas male urethral and foreskin microbiomes did not differ significantly.
    Discussion: We identified that a urinary microbiome is established as early as infancy. In addition, the female urobiome changes throughout childhood, until the post-pubertal bacterial taxa becomes consistent with that seen in adult females. Whereas in boys, the urinary microbiome changed very little over time. In addition, the surrounding urogenital microbiomes differed less in boys as compared to females. Microbiomes established at a young age may have long-term influences on immune, metabolic, and neurobehavioral traits. The same may be true for the urobiome. Our study provides a foundation for future research to determine the influence of the pediatric urobiome on the development of urinary and even non-urinary disorders.
    Conclusions: A pediatric urobiome exists, with differences between males and females and can be detected at a young age with changes occurring throughout childhood. Similarities and differences are also seen between the pediatric urobiome and adjacent niches.
    MeSH term(s) Adolescent ; Adult ; Bacteria ; Child ; Female ; Humans ; Male ; Microbiota/genetics ; Pilot Projects ; RNA, Ribosomal, 16S/genetics ; Urethra ; Urinary Bladder ; Urine/microbiology
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2022-03-04
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2237683-5
    ISSN 1873-4898 ; 1477-5131
    ISSN (online) 1873-4898
    ISSN 1477-5131
    DOI 10.1016/j.jpurol.2022.02.025
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  3. Article: Mechanisms by which statins protect endothelial cells from radiation-induced injury in the carotid artery.

    Ait-Aissa, Karima / Leng, Linette N / Lindsey, Nathanial R / Guo, Xutong / Juhr, Denise / Koval, Olha M / Grumbach, Isabella M

    Frontiers in cardiovascular medicine

    2023  Volume 10, Page(s) 1133315

    Abstract: Background: The incidental use of statins during radiation therapy has been associated with a reduced long-term risk of developing atherosclerotic cardiovascular disease. However, the mechanisms by which statins protect the vasculature from irradiation ... ...

    Abstract Background: The incidental use of statins during radiation therapy has been associated with a reduced long-term risk of developing atherosclerotic cardiovascular disease. However, the mechanisms by which statins protect the vasculature from irradiation injury remain poorly understood.
    Objectives: Identify the mechanisms by which the hydrophilic and lipophilic statins pravastatin and atorvastatin preserve endothelial function after irradiation.
    Methods: Cultured human coronary and umbilical vein endothelial cells irradiated with 4 Gy and mice subjected to 12 Gy head-and-neck irradiation were pretreated with statins and tested for endothelial dysfunction, nitric oxide production, oxidative stress, and various mitochondrial phenotypes at 24 and 240 h after irradiation.
    Results: Both pravastatin (hydrophilic) and atorvastatin (lipophilic) were sufficient to prevent the loss of endothelium-dependent relaxation of arteries after head-and-neck irradiation, preserve the production of nitric oxide by endothelial cells, and suppress the cytosolic reactive oxidative stress associated with irradiation. However, only pravastatin inhibited irradiation-induced production of mitochondrial superoxide; damage to the mitochondrial DNA; loss of electron transport chain activity; and expression of inflammatory markers.
    Conclusions: Our findings reveal some mechanistic underpinnings of the vasoprotective effects of statins after irradiation. Whereas both pravastatin and atorvastatin can shield from endothelial dysfunction after irradiation, pravastatin additionally suppresses mitochondrial injury and inflammatory responses involving mitochondria. Clinical follow-up studies will be necessary to determine whether hydrophilic statins are more effective than their lipophilic counterparts in reducing the risk of cardiovascular disease in patients undergoing radiation therapy.
    Language English
    Publishing date 2023-06-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2781496-8
    ISSN 2297-055X
    ISSN 2297-055X
    DOI 10.3389/fcvm.2023.1133315
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  4. Article ; Online: Reply by Authors.

    Gutierrez, Wade R / Luo, Yi / Dahmoush, Laila / Oleson, Jacob J / Schlaepfer, Charles H / Breyer, Benjamin N / Elliott, Sean P / Myers, Jeremy B / Vanni, Alex J / Juhr, Denise / Christel, Katherine N / Erickson, Bradley A

    The Journal of urology

    2024  , Page(s) 101097JU0000000000004003

    Language English
    Publishing date 2024-05-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3176-8
    ISSN 1527-3792 ; 0022-5347
    ISSN (online) 1527-3792
    ISSN 0022-5347
    DOI 10.1097/JU.0000000000004003
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  5. Article ; Online: Deep Phenotyping the Anterior Urethral Stricture: Characterizing the Relationship Between Inflammation, Fibrosis, Patient History, and Disease Pathophysiology.

    Gutierrez, Wade R / Luo, Yi / Dahmoush, Laila / Oleson, Jacob J / Schlaepfer, Charles H / Breyer, Benjamin N / Elliott, Sean P / Myers, Jeremy B / Vanni, Alex J / Juhr, Denise / Christel, Katherine N / Erickson, Bradley A

    The Journal of urology

    2024  , Page(s) 101097JU0000000000003962

    Abstract: Purpose: Anterior urethral stricture disease (aUSD) is a complex, heterogeneous condition that is idiopathic in origin for most men. This gap in knowledge rarely affects the current management strategy for aUSD, as urethroplasty does not generally ... ...

    Abstract Purpose: Anterior urethral stricture disease (aUSD) is a complex, heterogeneous condition that is idiopathic in origin for most men. This gap in knowledge rarely affects the current management strategy for aUSD, as urethroplasty does not generally consider etiology. However, as we transition towards personalized, minimally invasive treatments for aUSD and begin to consider aUSD prevention strategies, disease pathophysiology will become increasingly important. The purpose of this study was to perform a deep phenotype of men undergoing anterior urethroplasty for aUSD. We hypothesized that unique biologic signatures and potential targets for intervention would emerge based on stricture presence/absence, stricture etiology, and the presence/absence of stricture inflammation.
    Materials and methods: Men with aUSD undergoing urethroplasty were recruited from one of 5 participating centers. Enrollees provided urethral stricture tissue and blood/serum on the day of surgery and completed patient-reported outcome measure questionnaires both pre- and postoperatively. The initial study had 3 aims: (1) to determine pediatric and adult subacute and repeated perineal trauma (SRPT) exposures using a study-specific SRPT questionnaire, (2) to determine the degree of inflammation and fibrosis in aUSD and peri-aUSD (normal urethra) tissue, and (3) to determine levels of systemic inflammatory and fibrotic cytokines. Two controls groups provided serum (normal vasectomy patients) and urethral tissue (autopsy patients). Cohorts were based on the presence/absence of stricture, by presumed stricture etiology (idiopathic, traumatic/iatrogenic, lichen sclerosus [LS]), and by the presence/absence of stricture inflammation.
    Results: Of 138 enrolled men (120 tissue/serum; 18 stricture tissue only), 78 had idiopathic strictures, 33 had trauma-related strictures, and 27 had LS-related strictures. BMI, stricture length, and stricture location significantly differed between cohorts (
    Conclusions: The most common aUSD etiology is idiopathic. Though convention has implicated SRPT as causative for idiopathic strictures, here we found that patients with idiopathic strictures had low SRPT rates that were similar to rates in patients with a known stricture etiology. Stricture and stricture-adjacent inflammation in idiopathic stricture were similar to LS strictures, suggesting shared pathophysiologic mechanisms. IL-9, platelet-derived growth factor-BB, and CCL5, which were elevated in patients with strictures, have been implicated in fibrotic conditions elsewhere in the body. Further work will be required to determine if this shared biologic signature represents a potential mechanism for an aUSD predisposition.
    Language English
    Publishing date 2024-04-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3176-8
    ISSN 1527-3792 ; 0022-5347
    ISSN (online) 1527-3792
    ISSN 0022-5347
    DOI 10.1097/JU.0000000000003962
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  6. Article: Barriers to receiving testosterone replacement therapy in the community during COVID-19 pandemic.

    Troesch, Victoria L / Juhr, Denise / Pearlman, Amy

    Translational andrology and urology

    2018  Volume 10, Issue 8, Page(s) 3312–3316

    Abstract: Background: While the etiology for the upsurge in testosterone testing and prescriptions is likely multifactorial, increased direct-to-consumer marketing and the expansion of clinical care centers devoted to testosterone treatment likely play a role. ... ...

    Abstract Background: While the etiology for the upsurge in testosterone testing and prescriptions is likely multifactorial, increased direct-to-consumer marketing and the expansion of clinical care centers devoted to testosterone treatment likely play a role. Many of these centers require patients to report, in-person, on a regular basis for their injectable therapy and/or lab studies. The purpose of our study was to investigate barriers of care that patients receiving treatment for testosterone deficiency may be experiencing in the setting of COVID-19.
    Methods: Our survey was posted on a closed Facebook support page for males currently receiving testosterone treatment and members of the group were invited to participate. The survey asked participants several questions related to how they received their injections, if they've experienced difficulties obtaining their injections due to COVID-19 restrictions, and about their interest in telemedicine services for their care.
    Results: The majority of patients were able to receive their treatment despite barriers enforced by the pandemic. Of the 104 participants, almost half received their testosterone prescriptions from an outpatient clinic dedicated to testosterone replacement, while the other half received their therapy from a PCP, endocrinologist, or urologist. Only 5 patients (4.8%) noted difficulties obtaining their injections during this pandemic, 4 of which received their prescriptions from dedicated testosterone clinics, and the other from a PCP. Nearly 90% of respondents self-administered their testosterone therapy. With regards to telemedicine, 57.8% of patients have utilized the technology in some capacity, however 74.4% said that they would prefer to use telemedicine video services with a urologist or APP with expertise in andrology over in-person services.
    Conclusions: In our survey, the majority of the respondents have been able to receive their injectable testosterone therapy despite the ongoing pandemic. The majority of respondents self-administer their treatments, which may explain the lack of barriers. This study is the first of its kind to investigate the effect of a pandemic on the receipt of care for those being treated for testosterone deficiency with injectable testosterone.
    Language English
    Publishing date 2018-04-01
    Publishing country China
    Document type Journal Article
    ZDB-ID 2851630-8
    ISSN 2223-4691 ; 2223-4691 ; 2223-4683
    ISSN (online) 2223-4691
    ISSN 2223-4691 ; 2223-4683
    DOI 10.21037/tau-21-350
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  7. Article ; Online: Pilot study of a home use cystomanometer in patients with a neurogenic bladder.

    Cooper, Christopher S / Bonnett, Megan A / Ortman, Christopher E / Juhr, Denise / Storm, Douglas W / Lockwood, Gina M

    Journal of pediatric urology

    2022  Volume 18, Issue 4, Page(s) 466–468

    Abstract: Introduction: A novel device, the cystomanometer, was developed for home bladder pressure monitoring in patients with neurogenic bladder.: Objective: To report initial experience and proof of concept with home use of the cystomanometer.: Study ... ...

    Abstract Introduction: A novel device, the cystomanometer, was developed for home bladder pressure monitoring in patients with neurogenic bladder.
    Objective: To report initial experience and proof of concept with home use of the cystomanometer.
    Study design: Patients were asked to use the device twice daily for two weeks.
    Results: Fourteen patients with neurogenic bladder were enrolled.
    Discussion: The cystomanometer initially functioned well and transmitted data to a smartphone and to the hospital server. However, over 50% of devices broke.
    Conclusions: We report the first home use of a handheld electronic cystomanometer with wireless data transmission to a smartphone and hospital database.
    MeSH term(s) Humans ; Urinary Bladder, Neurogenic/diagnosis ; Pilot Projects ; Urodynamics ; Urinary Bladder
    Language English
    Publishing date 2022-06-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2237683-5
    ISSN 1873-4898 ; 1477-5131
    ISSN (online) 1873-4898
    ISSN 1477-5131
    DOI 10.1016/j.jpurol.2022.05.023
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  8. Article ; Online: Opioids and pediatric urology: A prospective study evaluating prescribing habits and patient postoperative pain and narcotic utilization.

    Lence, Tomas / Thinnes, Robert / Foster, Alexander J / Cooper, Christopher S / Lockwood, Gina M / Eyck, Patrick Ten / Rye, Zachary / Wu, Chaorong / Juhr, Denise / Storm, Douglas W

    Journal of pediatric urology

    2023  Volume 19, Issue 3, Page(s) 295.e1–295.e8

    Abstract: Introduction: Few pediatric urologists believe patients require a majority of the doses of opioids prescribed to them postoperatively. Seeking a better understanding of postoperative pain and analgesia in pediatric urology patients may help reduce ... ...

    Abstract Introduction: Few pediatric urologists believe patients require a majority of the doses of opioids prescribed to them postoperatively. Seeking a better understanding of postoperative pain and analgesia in pediatric urology patients may help reduce opioid over prescription while still adequately managing postoperative pain.
    Objective: We sought to better understand: 1) the postoperative pain levels experienced by pediatric urology patients, 2) the factors that correlate with postoperative pain and number of opioids consumed following pediatric urologic procedures, and 3) the patients who do not require opioids after surgery.
    Study design: Pediatric patients undergoing circumcision, inguinal hernia repair, orchidopexy, or hypospadias repair were eligible to participate. Patients were enrolled in the prospective cohort on the day of the procedure. For each of the first 7 postoperative days, patients' parents completed a text message-based questionnaire, quantifying their child's pain level and the doses of pain medication the child consumed.
    Results: 165 participants were enrolled. 57 patients underwent circumcision, 54 underwent orchiopexy, 32 underwent hypospadias repair, and 22 underwent inguinal hernia repair. For all procedure types, pain scores (p < 0.01) and doses of oxycodone consumed were highest on postoperative day one and steadily declined thereafter. Overall, average 7-day pain score (2.02; 0.86-5.14) and doses of narcotics consumed (3.50; 0-5) were low. Patients in each surgical subgroup were prescribed narcotics in excess of what was consumed. There was an average excess of 10.9 doses (0-39.0) for hypospadias repair, 8.6 (1.0-30.0) for circumcision, 9.0 (3.0-21.0) for inguinal hernia repair, and 6.1 (0-22.0) for orchiopexy.
    Discussion: Overall, reported pain scores and number of narcotics consumed were low regardless of surgery type. Opioids were overprescribed regardless of surgery type.
    Conclusions: Our findings indicate that level of pain and opioid use varies by procedure type, but that number of narcotics prescribed greatly exceeds number needed.
    MeSH term(s) Male ; Humans ; Child ; Analgesics, Opioid/therapeutic use ; Prospective Studies ; Narcotics/therapeutic use ; Urology ; Hernia, Inguinal/surgery ; Hypospadias/drug therapy ; Pain, Postoperative/drug therapy ; Habits ; Practice Patterns, Physicians'
    Chemical Substances Analgesics, Opioid ; Narcotics
    Language English
    Publishing date 2023-01-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2237683-5
    ISSN 1873-4898 ; 1477-5131
    ISSN (online) 1873-4898
    ISSN 1477-5131
    DOI 10.1016/j.jpurol.2022.12.018
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  9. Article ; Online: How Effective Was the 2014 AUA Cryptorchidism Guideline? A Multi-institutional Evaluation.

    Weiner, Hillary / Solomon, Julie R / Thinnes, Robert / Pinsky, Benjamin / Ferreri, Charles / Singleterry, Marquise / Bahamonde, Amanda / Awadh, Sami / Tran, Jacqueline / Paradis, Alethea G / Vetter, Joel / Brooks, Anthony / Lund, Samantha / Kuwaya, Daren / Juhr, Denise / Wendt, Linder / Eyck, Patrick Ten / Traxel, Erica / Kraft, Kate H /
    Ellison, Jonathan S / Storm, Douglas W

    Urology practice

    2023  Volume 10, Issue 6, Page(s) 605–610

    Abstract: Introduction: In 2014, the AUA published guidelines regarding the evaluation of cryptorchidism. This multi-institutional study aims to determine if these guidelines reduced the age of referral and the utilization of ultrasound in boys with ... ...

    Abstract Introduction: In 2014, the AUA published guidelines regarding the evaluation of cryptorchidism. This multi-institutional study aims to determine if these guidelines reduced the age of referral and the utilization of ultrasound in boys with cryptorchidism. We hypothesize that delayed referral continues, and utilization of ultrasound remains unchanged.
    Methods: A retrospective review of boys referred for the evaluation of cryptorchidism was performed at 4 academic institutions, collecting data for 1 year prior (2013) and 2 nonconsecutive years following guideline creation (2015 and 2019). Across these time frames, we compared median ages at evaluation and surgery, and rates of patient comorbidities, orchiopexy, and preevaluation ultrasound.
    Results: A total of 3,293 patients were included. The median age at initial pediatric urology evaluation in all cohorts was 39 months (IQR: 14-92 months). Following publication of the AUA Guidelines, there was no difference (
    Conclusions: Our data suggest that, despite publication of the AUA Guidelines on evaluation and treatment of cryptorchidism, there has been no reduction in the age of urological evaluation or the utilization of imaging in boys with undescended testis. Finding alternative avenues to disseminate these evidence-based recommendations to referring providers and exploring barriers to guideline adherence is necessary to improve care for patients with cryptorchidism.
    MeSH term(s) Male ; Humans ; Child ; Infant ; Child, Preschool ; Cryptorchidism/diagnosis ; Referral and Consultation ; Orchiopexy/methods ; Retrospective Studies ; Ultrasonography
    Language English
    Publishing date 2023-07-27
    Publishing country United States
    Document type Multicenter Study ; Journal Article
    ISSN 2352-0787
    ISSN (online) 2352-0787
    DOI 10.1097/UPJ.0000000000000437
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  10. Article ; Online: The E3 ubiquitin ligase Asb2β is downregulated in a mouse model of hypertrophic cardiomyopathy and targets desmin for proteasomal degradation.

    Thottakara, Tilo / Friedrich, Felix W / Reischmann, Silke / Braumann, Simon / Schlossarek, Saskia / Krämer, Elisabeth / Juhr, Denise / Schlüter, Hartmut / van der Velden, Jolanda / Münch, Julia / Patten, Monica / Eschenhagen, Thomas / Moog-Lutz, Christel / Carrier, Lucie

    Journal of molecular and cellular cardiology

    2015  Volume 87, Page(s) 214–224

    Abstract: Background: Hypertrophic cardiomyopathy (HCM) is an autosomal-dominant disease with mutations in genes encoding sarcomeric proteins. Previous findings suggest deregulation of the ubiquitin proteasome system (UPS) in HCM in humans and in a mouse model of ...

    Abstract Background: Hypertrophic cardiomyopathy (HCM) is an autosomal-dominant disease with mutations in genes encoding sarcomeric proteins. Previous findings suggest deregulation of the ubiquitin proteasome system (UPS) in HCM in humans and in a mouse model of HCM (Mybpc3-targeted knock-in (KI) mice). In this study we investigated transcript levels of several muscle-specific E3 ubiquitin ligases in KI mice and aimed at identifying novel protein targets.
    Methods and results: Out of 9 muscle-specific E3 ligases, Asb2β was found with the lowest mRNA level in KI compared to wild-type (WT) mice. After adenoviral-mediated Asb2β transduction of WT neonatal mouse cardiomyocytes with either a WT or inactive Asb2β mutant, desmin was identified as a new target of Asb2β by mass spectrometry, co-immunoprecipitation and immunoblotting. Immunofluorescence analysis revealed a co-localization of desmin with Asb2β at the Z-disk of the sarcomere. Knock-down of Asb2β in cardiomyocytes resulted in higher desmin protein levels. Furthermore, desmin levels were higher in ventricular samples of HCM mice and patients than controls.
    Conclusions: This study identifies desmin as a new Asb2β target for proteasomal degradation in cardiomyocytes and suggests that accumulation of desmin could contribute to UPS impairment in HCM mice and patients.
    MeSH term(s) Adaptor Proteins, Signal Transducing/biosynthesis ; Adaptor Proteins, Signal Transducing/genetics ; Animals ; Cardiomyopathy, Hypertrophic/genetics ; Cardiomyopathy, Hypertrophic/pathology ; Desmin/genetics ; Desmin/metabolism ; Disease Models, Animal ; Gene Expression Regulation ; Humans ; Mice ; Mutation ; Myocardium/metabolism ; Myocardium/pathology ; Myocytes, Cardiac/metabolism ; Myocytes, Cardiac/pathology ; Proteasome Endopeptidase Complex/metabolism ; Proteolysis ; Sarcomeres/metabolism ; Ubiquitin
    Chemical Substances Adaptor Proteins, Signal Transducing ; Asb2 protein, mouse ; Desmin ; Ubiquitin ; Proteasome Endopeptidase Complex (EC 3.4.25.1)
    Language English
    Publishing date 2015-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80157-4
    ISSN 1095-8584 ; 0022-2828
    ISSN (online) 1095-8584
    ISSN 0022-2828
    DOI 10.1016/j.yjmcc.2015.08.020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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