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  1. Article ; Online: Bis(μ2-diphenylphosphinamide-κ2O:O)bis[bis(diphenylphosphinamide-κO)lithium] dichloride acetonitrile disolvate

    Ai-Hong Li / Jun-Ping Han / Jing Li

    Acta Crystallographica Section E, Vol 70, Iss 6, Pp m224-m

    2014  Volume 224

    Abstract: The asymmetric unit of the title compound, [Li2(C12H12NOP)6]Cl2·2CH3CN, contains one-half of the centrosymmetric dication, one chloride anion and one acetonitrile solvent molecule. Each Li atom is coordinated by four O atoms [Li—O 1.891 (3) and 2.025 (3) ...

    Abstract The asymmetric unit of the title compound, [Li2(C12H12NOP)6]Cl2·2CH3CN, contains one-half of the centrosymmetric dication, one chloride anion and one acetonitrile solvent molecule. Each Li atom is coordinated by four O atoms [Li—O 1.891 (3) and 2.025 (3) Å] from the four diphenylphosphinamide ligands in a distorted tetrahedral geometry. In the crystal, weak N—H.Cl hydrogen bonds link the anions and dications into columns extending along [100].
    Keywords Chemistry ; QD1-999
    Language English
    Publishing date 2014-06-01T00:00:00Z
    Publisher International Union of Crystallography
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: DNA profiling of spermatozoa by laser capture microdissection and low volume-PCR.

    Cai-xia Li / Jun-ping Han / Wen-yan Ren / An-quan Ji / Xiu-lan Xu / Lan Hu

    PLoS ONE, Vol 6, Iss 8, p e

    2011  Volume 22316

    Abstract: Genetic profiling of sperm from complex biological mixtures such as sexual assault casework samples requires isolation of a pure sperm population and the ability to analyze low abundant samples. Current standard procedure for sperm isolation includes ... ...

    Abstract Genetic profiling of sperm from complex biological mixtures such as sexual assault casework samples requires isolation of a pure sperm population and the ability to analyze low abundant samples. Current standard procedure for sperm isolation includes preferential lysis of epithelial contaminants followed by collection of intact sperm by centrifugation. While effective for samples where sperm are abundant, this method is less effective when samples contain few spermatozoa. Laser capture microdissection (LCM) is a proven method for the isolation of cells biological mixtures, even when found in low abundance. Here, we demonstrate the efficacy of LCM coupled with on-chip low volume PCR (LV-PCR) for the isolation and genotyping of low abundance sperm samples. Our results indicate that this method can obtain complete profiles (13-16 loci) from as few as 15 sperm cells with 80% reproducibility, whereas at least 40 sperm cells are required to profile 13-16 loci by standard 'in-tube' PCR. Further, LCM and LV-PCR of a sexual assault casework sample generated a DNA genotype that was consistent with that of the suspect. This method was unable, however, to analyze a casework sample from a gang rape case in which two or more sperm contributors were in a mixed population. The results indicate that LCM and LV-PCR is sensitive and effective for genotyping sperm from sperm/epithelial cell mixtures when epithelial lysis may be insufficient due to low abundance of sperm; LCM and LV-PCR, however, failed in a casework sample when spermatozoa from multiple donors was present, indicating that further study is necessitated.
    Keywords Medicine ; R ; Science ; Q
    Subject code 630
    Language English
    Publishing date 2011-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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