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  1. Article ; Online: Surgery-related anxiety on geriatric patients undergoing total knee arthroplasty: a retrospective observational study.

    Jung, Kwang-Hwan / Park, Jang-Ho / Ahn, Jae-Woo / Park, Ki-Bong

    BMC musculoskeletal disorders

    2023  Volume 24, Issue 1, Page(s) 161

    Abstract: Background: The prevalence of anxiety in patients undergoing total knee arthroplasty (TKA) and its association with postoperative functions are well known; however, the levels of anxiety or anxiety-related characteristics are unknown. This study aimed ... ...

    Abstract Background: The prevalence of anxiety in patients undergoing total knee arthroplasty (TKA) and its association with postoperative functions are well known; however, the levels of anxiety or anxiety-related characteristics are unknown. This study aimed to investigate the prevalence of clinically significant state anxiety in geriatric patients undergoing TKA for osteoarthritis (OA) of the knee and to evaluate the anxiety-related characteristics experienced by these patients pre- and post-operatively.
    Methods: This retrospective observational study recruited patients who had undergone TKA for knee OA using general anesthesia between February 2020 and August 2021. The study participants were geriatric patients older than 65 years who had moderate or severe OA. We evaluated patient characteristics including age, sex, body mass index, smoking status, hypertension, diabetes, and cancer. We assessed their levels of anxiety status using the STAI-X which comprises 20-item scales. Clinically meaningful state anxiety was defined as a total score of 52 or higher. An independent Student's t-test was used to determine differences of STAI score between subgroups in terms of patient characteristics. And patients were asked to complete questionnaires, which assessed four areas: (1) the main cause of anxiety; (2) the most helpful factor in overcoming anxiety before surgery; (3) the most helpful factor in reducing anxiety after surgery; and (4) the most anxious moment during the entire process.
    Results: The mean STAI score of patients who underwent TKA was 43.0 points and 16.4% of patients experienced clinically significant state anxiety. The current smoking status affect STAI score and the proportion of patients with clinically meaningful state anxiety. The most common cause of preoperative anxiety was the surgery itself. Overall, 38% of patients reported that they experienced the greatest level of anxiety when the surgeon had recommended TKA in the outpatient clinic. The trust in the medical staff before surgery and the surgeon's explanations after surgery helped the most in reducing anxiety.
    Conclusions: One in six patients before TKA experience clinically meaningful state anxiety, and about 40% of patients experience anxiety from the time they are recommended for surgery. Patients tended to overcome anxiety before TKA through trust in the medical staff, and the surgeon's explanations after surgery was found to be helpful in reducing anxiety.
    MeSH term(s) Humans ; Aged ; Arthroplasty, Replacement, Knee/adverse effects ; Anxiety/diagnosis ; Anxiety/epidemiology ; Anxiety/etiology ; Anxiety Disorders ; Knee Joint ; Osteoarthritis, Knee/epidemiology ; Osteoarthritis, Knee/surgery
    Language English
    Publishing date 2023-03-03
    Publishing country England
    Document type Observational Study ; Journal Article
    ZDB-ID 2041355-5
    ISSN 1471-2474 ; 1471-2474
    ISSN (online) 1471-2474
    ISSN 1471-2474
    DOI 10.1186/s12891-023-06252-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Predictive Variables for Interventional Angiography among Patients with Knee Hemarthrosis.

    Ko, Sang-Hun / Jung, Kwang-Hwan / Cha, Jae-Ryong / Park, Ki-Bong

    Diagnostics (Basel, Switzerland)

    2022  Volume 12, Issue 4

    Abstract: Studies regarding the variables that could predict the success of conservative treatment for knee hemarthrosis are lacking. This retrospective study evaluated the laboratory variables of patients who had unsatisfactory results from conservative treatment ...

    Abstract Studies regarding the variables that could predict the success of conservative treatment for knee hemarthrosis are lacking. This retrospective study evaluated the laboratory variables of patients who had unsatisfactory results from conservative treatment for knee hemarthrosis. Twenty-nine patients conservatively treated for knee hemarthrosis were included and divided into two groups: group A comprised 14 patients who underwent interventional angiography and selective embolization due to failed conservative treatment, and group B comprised 15 patients with successful results after conservative treatment. The results of the serological and synovial fluid tests were evaluated. The mean number of synovial red blood cells (RBCs) was 1,905,857 cells/µL and 7730 cells/µL in groups A and B, respectively (p = 0.01), while the mean number of RBCs per high-power field (HPF) was 68.9 and 3.2, respectively (p < 0.01). Patients who underwent interventional angiography and selective embolization after failed conservative treatment for knee hemarthrosis had higher synovial RBC counts and RBC counts per HPF than those with successful outcomes after conservative treatment. It is necessary to carefully interpret the results of the synovial fluid analysis in patients with knee hemarthrosis; if the synovial fluid analysis shows a synovial RBC count greater than 81,500 and RBC count per HPF greater than 16.3, we recommend immediate interventional angiography rather than continuing conservative treatment.
    Language English
    Publishing date 2022-04-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics12040976
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: A Bibliometric Analysis of the Field of Computer-Assisted Orthopedic Surgery during 2002-2021.

    Lee, Chae-Chil / Jung, Kwang-Hwan / Lee, Kyung-Joo / Park, Ki-Bong

    Clinics in orthopedic surgery

    2022  Volume 15, Issue 2, Page(s) 227–233

    Abstract: Background: This study aimed to investigate the characteristics of research articles and research trends in computer-assisted orthopedic surgery (CAOS) by conducting bibliometric analyses.: Methods: CAOS-related research articles published in ... ...

    Abstract Background: This study aimed to investigate the characteristics of research articles and research trends in computer-assisted orthopedic surgery (CAOS) by conducting bibliometric analyses.
    Methods: CAOS-related research articles published in international journals from 2002 to 2021 were collected using the PubMed database and analyzed using the bibliometric method. Their publication year, journal name, corresponding author's country name, and the number of citations of all collected articles were noted. Contents of the articles were analyzed to evaluate the time point and anatomical site at which the digital technique was applied. Further, the 20-year period was divided into two halves of 10 years each to analyze the research trends.
    Results: A total of 639 CAOS-related articles were identified. An average of 32.0 CAOS-related articles were published annually, with an average of 20.6 and 43.3 published in the first half and second half, respectively. Of all articles, 47.6% were published in the top 10 journals, and 81.2% were written in the top 10 countries. The total numbers of citations were 11.7 and 6.3 in the first and second halves, respectively, but the average annual number of citations was higher in the second half than in the first one. Articles on application of digital techniques during surgery were 62.3% and those on pre-surgery application were 36.9%. Further, articles in the knee (39.0%), spine (28.5%), and hip and pelvis (21.5%) fields accounted for 89.0% of the total publications. But the increase in publications in the said period was highest in the fields of the hand and wrist (+1,300.0%), ankle (+466.7%), and shoulder (+366.7%).
    Conclusions: Over the last 20 years, the publication of CAOS-related research articles in international journals has grown steadily. Although the knee, spine, hip, and pelvis fields account for most CAOS-related research, research in new fields is also increasing. This study analyzed the types of articles and trends in CAOS-related research and provided useful information for future research in the field of CAOS.
    MeSH term(s) Humans ; Bibliometrics ; Orthopedics ; Spine/surgery ; Orthopedic Procedures ; Computers
    Language English
    Publishing date 2022-08-16
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2502788-8
    ISSN 2005-4408 ; 2005-291X
    ISSN (online) 2005-4408
    ISSN 2005-291X
    DOI 10.4055/cios21217
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Real-time Visualization of Transcribed mRNA via Click Chemistry in a Liposomal Space.

    Jeon, Seonmin / Ros, Casana / Huh, Eunjin / Choi, Hyun Kyung / Cho, Shin-Gyu / Jung, Kwang-Hwan / Shin, Kwanwoo

    Journal of oleo science

    2024  Volume 73, Issue 4, Page(s) 573–581

    Abstract: We present a CuAAC (Copper-Catalyzed Azide-Alkyne Cycloaddition) reaction protocol designed for the visualization of mRNA. To achieve this, we synthesized stable mRNA molecules incorporating the modified nucleoside analog, EU, a crucial element for ... ...

    Abstract We present a CuAAC (Copper-Catalyzed Azide-Alkyne Cycloaddition) reaction protocol designed for the visualization of mRNA. To achieve this, we synthesized stable mRNA molecules incorporating the modified nucleoside analog, EU, a crucial element for fluorophore attachment. Leveraging this modified mRNA, we successfully executed the CuAAC reaction, wherein the pro-fluorophore, coumarin, was conjugated to EU on the mRNA through our meticulously designed CuAAC process. This innovative approach resulted in the emission of fluorescence, enabling both precise quantification and visual observation of mRNA. Furthermore, we demonstrated the feasibility of concurrent mRNA synthesis and visualization by seamlessly integrating the CuAAC reaction mix into the mRNA transcription process. Additionally, our novel methodology opens avenues for prospective real-time monitoring of mRNA transcription within artificial cells. These advancements hold significant promise for expanding our comprehension of fundamental cellular processes and finding applications across diverse biological contexts in the future.
    MeSH term(s) Click Chemistry/methods ; Prospective Studies ; Azides/chemistry ; Copper/chemistry ; Cycloaddition Reaction ; Catalysis
    Chemical Substances Azides ; Copper (789U1901C5)
    Language English
    Publishing date 2024-04-01
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2218264-0
    ISSN 1347-3352 ; 1345-8957
    ISSN (online) 1347-3352
    ISSN 1345-8957
    DOI 10.5650/jos.ess23198
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Retinal-Carotenoid Interactions in a Sodium-Ion-Pumping Rhodopsin: Implications on Oligomerization and Thermal Stability.

    Ghosh, Mihir / Misra, Ramprasad / Bhattacharya, Sudeshna / Majhi, Koushik / Jung, Kwang-Hwan / Sheves, Mordechai

    The journal of physical chemistry. B

    2023  Volume 127, Issue 10, Page(s) 2128–2137

    Abstract: Microbial rhodopsin (also called retinal protein)-carotenoid conjugates represent a unique class of light-harvesting (LH) complexes, but their specific interactions and LH properties are not completely elucidated as only few rhodopsins are known to bind ... ...

    Abstract Microbial rhodopsin (also called retinal protein)-carotenoid conjugates represent a unique class of light-harvesting (LH) complexes, but their specific interactions and LH properties are not completely elucidated as only few rhodopsins are known to bind carotenoids. Here, we report a natural sodium-ion (Na
    MeSH term(s) Rhodopsin/chemistry ; Sodium/metabolism ; Carotenoids/chemistry ; Retina/chemistry ; Rhodopsins, Microbial/chemistry
    Chemical Substances Rhodopsin (9009-81-8) ; Sodium (9NEZ333N27) ; salinixanthin ; Carotenoids (36-88-4) ; Rhodopsins, Microbial
    Language English
    Publishing date 2023-03-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1520-5207
    ISSN (online) 1520-5207
    DOI 10.1021/acs.jpcb.2c07502
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: The chirality origin of retinal-carotenoid complex in gloeobacter rhodopsin: a temperature-dependent excitonic coupling.

    Jana, Sankar / Jung, Kwang-Hwan / Sheves, Mordechai

    Scientific reports

    2020  Volume 10, Issue 1, Page(s) 13992

    Abstract: Retinal proteins play significant roles in light-induced protons/ions transport across the cell membrane. A recent studied retinal protein, gloeobacter rhodopsin (gR), functions as a proton pump, and binds the carotenoid salinixanthin (sal) in addition ... ...

    Abstract Retinal proteins play significant roles in light-induced protons/ions transport across the cell membrane. A recent studied retinal protein, gloeobacter rhodopsin (gR), functions as a proton pump, and binds the carotenoid salinixanthin (sal) in addition to the retinal chromophore. We have studied the interactions between the two chromophores as reflected in the circular dichroism (CD) spectrum of gR complex. gR exhibits a weak CD spectrum but following binding of sal, it exhibits a significant enhancement of the CD bands. To examine the CD origin, we have substituted the retinal chromophore of gR by synthetic retinal analogues, and have concluded that the CD bands originated from excitonic interaction between sal and the retinal chromophore as well as the sal chirality induced by binding to the protein. Temperature increase significantly affected the CD spectra, due to vanishing of excitonic coupling. A similar phenomenon of excitonic interaction lose between chromophores was recently reported for a photosynthetic pigment-protein complex (Nature Commmun, 9, 2018, 99). We propose that the excitonic interaction in gR is weaker due to protein conformational alterations. The excitonic interaction is further diminished following reduction of the retinal protonated Schiff base double bond. Furthermore, the intact structure of the retinal ring is necessary for obtaining the excitonic interaction.
    MeSH term(s) Carotenoids/chemistry ; Carotenoids/metabolism ; Cyanobacteria/metabolism ; Glycosides/chemistry ; Glycosides/metabolism ; Hydrogen-Ion Concentration ; Models, Molecular ; Molecular Structure ; Protein Binding ; Protein Conformation ; Proton Pumps/chemistry ; Proton Pumps/metabolism ; Retina/metabolism ; Retinaldehyde/chemistry ; Retinaldehyde/metabolism ; Rhodopsin/chemistry ; Rhodopsin/metabolism ; Rhodopsins, Microbial/chemistry ; Rhodopsins, Microbial/metabolism ; Schiff Bases/chemistry ; Stereoisomerism ; Temperature
    Chemical Substances Glycosides ; Proton Pumps ; Rhodopsins, Microbial ; Schiff Bases ; salinixanthin ; Carotenoids (36-88-4) ; Rhodopsin (9009-81-8) ; Retinaldehyde (RR725D715M)
    Language English
    Publishing date 2020-08-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-70697-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Does spiked tibial cement spacer reduce spacer-related problems in two-stage revision total knee arthroplasty for infection?

    Jung, Kwang-Hwan / Lee, Chae-Chil / Kim, Tae-Hoon / Han, Jung-Won / Park, Ki-Bong

    International orthopaedics

    2022  Volume 46, Issue 9, Page(s) 2009–2017

    Abstract: Purpose: Articulating cement spacers are frequently used in staged approaches for infected total knee arthroplasty (TKA). This study investigated whether a tibial cement spacer (TCS) with spikes could reduce spacer-related problems in two-stage revision ...

    Abstract Purpose: Articulating cement spacers are frequently used in staged approaches for infected total knee arthroplasty (TKA). This study investigated whether a tibial cement spacer (TCS) with spikes could reduce spacer-related problems in two-stage revision TKA (R-TKA).
    Methods: A total of 27 patients (27 knees; 10 men and 17 women) who underwent two-stage R-TKA for infected TKA were retrospectively analyzed. Group A comprised 12 patients who used TCS with spikes added to the bottom surface, whereas group B consisted of 15 patients who used conventional TCS with a flat bottom. For each group, plain radiographs were obtained after cement spacer implantation and before R-TKA to measure mediolateral (ML) translation and TCS's tilting angle. Patients' demographic data, ML translation of the TCS, and changes in the TCS's tilting angle between the groups were analyzed.
    Results: The mean ML translation was significantly lower in group A than that in group B (1.7 mm vs. 5.4 mm, p = 0.04). The mean change in the tilting angle was significantly lower in group A than that in group B (4.5° vs. 19.4°, p = 0.047).
    Conclusion: The spiked TCS in two-stage R-TKA provides superior stability compared to the TCS with a conventional design.
    MeSH term(s) Anti-Bacterial Agents/therapeutic use ; Arthroplasty, Replacement, Knee/adverse effects ; Bone Cements ; Female ; Humans ; Knee Joint/surgery ; Knee Prosthesis/adverse effects ; Male ; Prosthesis-Related Infections/drug therapy ; Prosthesis-Related Infections/prevention & control ; Prosthesis-Related Infections/surgery ; Reoperation ; Retrospective Studies ; Treatment Outcome
    Chemical Substances Anti-Bacterial Agents ; Bone Cements
    Language English
    Publishing date 2022-05-18
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 80384-4
    ISSN 1432-5195 ; 0341-2695
    ISSN (online) 1432-5195
    ISSN 0341-2695
    DOI 10.1007/s00264-022-05438-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Heliorhodopsin binds and regulates glutamine synthetase activity.

    Cho, Shin-Gyu / Song, Myungchul / Chuon, Kimleng / Shim, Jin-Gon / Meas, Seanghun / Jung, Kwang-Hwan

    PLoS biology

    2022  Volume 20, Issue 10, Page(s) e3001817

    Abstract: Photoreceptors are light-sensitive proteins found in various organisms that respond to light and relay signals into the cells. Heliorhodopsin, a retinal-binding membrane protein, has been recently discovered, however its function remains unknown. Herein, ...

    Abstract Photoreceptors are light-sensitive proteins found in various organisms that respond to light and relay signals into the cells. Heliorhodopsin, a retinal-binding membrane protein, has been recently discovered, however its function remains unknown. Herein, we investigated the relationship between Actinobacteria bacterium IMCC26103 heliorhodopsin (AbHeR) and an adjacent glutamine synthetase (AbGS) in the same operon. We demonstrate that AbHeR binds to AbGS and regulates AbGS activity. More specifically, the dissociation constant (Kd) value of the binding between AbHeR and AbGS is 6.06 μM. Moreover, the absence of positively charged residues within the intracellular loop of AbHeR impacted Kd value as they serve as critical binding sites for AbGS. We also confirm that AbHeR up-regulates the biosynthetic enzyme activity of AbGS both in vitro and in vivo in the presence of light. GS is a key enzyme involved in nitrogen assimilation that catalyzes the conversion of glutamate and ammonia to glutamine. Hence, the interaction between AbHeR and AbGS may be critical for nitrogen assimilation in Actinobacteria bacterium IMCC26103 as it survives in low-nutrient environments. Overall, the findings of our study describe, for the first time, to the best of our knowledge, a novel function of heliorhodopsin as a regulatory rhodopsin with the capacity to bind and regulate enzyme activity required for nitrogen assimilation.
    MeSH term(s) Ammonia/metabolism ; Glutamate-Ammonia Ligase/chemistry ; Glutamate-Ammonia Ligase/metabolism ; Glutamic Acid/metabolism ; Glutamine ; Nitrogen ; Rhodopsin ; Rhodopsins, Microbial
    Chemical Substances Rhodopsins, Microbial ; heliorhodopsin ; Glutamine (0RH81L854J) ; Glutamic Acid (3KX376GY7L) ; Ammonia (7664-41-7) ; Rhodopsin (9009-81-8) ; Glutamate-Ammonia Ligase (EC 6.3.1.2) ; Nitrogen (N762921K75)
    Language English
    Publishing date 2022-10-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2126776-5
    ISSN 1545-7885 ; 1544-9173
    ISSN (online) 1545-7885
    ISSN 1544-9173
    DOI 10.1371/journal.pbio.3001817
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Mutational analyses identify a single amino acid critical for colour tuning in proteorhodopsins.

    Shim, Jin-Gon / Kang, Na-Rae / Chuon, Kimleng / Cho, Shin-Gyu / Meas, Seanghun / Jung, Kwang-Hwan

    FEBS letters

    2022  Volume 596, Issue 6, Page(s) 784–795

    Abstract: Microbial rhodopsins are light-activated proteins that contain seven transmembrane alpha-helices. Spectral tuning in microbial rhodopsins is a useful optogenetic tool. In this study, we report a new site that controls spectral tuning. In the ... ...

    Abstract Microbial rhodopsins are light-activated proteins that contain seven transmembrane alpha-helices. Spectral tuning in microbial rhodopsins is a useful optogenetic tool. In this study, we report a new site that controls spectral tuning. In the proteorhodopsins ISR34 and ISR36, a single amino-acid substitution at Cys189 caused an absorption maximum shift of 44 nm, indicating spectral tuning at a specific site. Comparison of single amino acid substitutions was conducted using photochemical and photobiological approaches. The maximum absorption for red-shift was measured for mutations at positions 189 and 105 in ISR34, both residues being equally important. Structural changes resulting from amino acid substitutions are related to pK
    MeSH term(s) Amino Acid Sequence ; Amino Acids/genetics ; Color ; Rhodopsin/chemistry ; Rhodopsins, Microbial/metabolism
    Chemical Substances Amino Acids ; Rhodopsins, Microbial ; proteorhodopsin ; Rhodopsin (9009-81-8)
    Language English
    Publishing date 2022-02-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 212746-5
    ISSN 1873-3468 ; 0014-5793
    ISSN (online) 1873-3468
    ISSN 0014-5793
    DOI 10.1002/1873-3468.14297
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Impact of protein-chromophore interaction on the retinal excited state and photocycle of

    Misra, Ramprasad / Das, Ishita / Dér, András / Steinbach, Gábor / Shim, Jin-Gon / Busse, Wayne / Jung, Kwang-Hwan / Zimányi, László / Sheves, Mordechai

    Chemical science

    2023  Volume 14, Issue 36, Page(s) 9951–9958

    Abstract: The function of microbial as well as mammalian retinal proteins ( ...

    Abstract The function of microbial as well as mammalian retinal proteins (
    Language English
    Publishing date 2023-09-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2559110-1
    ISSN 2041-6539 ; 2041-6520
    ISSN (online) 2041-6539
    ISSN 2041-6520
    DOI 10.1039/d3sc02961a
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