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  1. Article ; Online: The influence of alkylating agents on sulfur-sulfur bonds in per- and polysulfides.

    Schilling, Danny / Ditrói, Tamás / Barayeu, Uladzimir / Jurányi, Eszter Petra / Nagy, Peter / Dick, Tobias P

    Current opinion in chemical biology

    2023  Volume 76, Page(s) 102368

    Abstract: Per- and polysulfides are sulfane sulfur species produced inside living cells, in organisms as diverse as bacteria, plants and humans, but their biological roles remain to be fully understood. Unfortunately, due to their reactivity, per- and polysulfides ...

    Abstract Per- and polysulfides are sulfane sulfur species produced inside living cells, in organisms as diverse as bacteria, plants and humans, but their biological roles remain to be fully understood. Unfortunately, due to their reactivity, per- and polysulfides are easily altered, interconverted or lost during the processing and analysis of biological material. Thus, all current analytical methods make use of alkylating agents, to quench reactivity of hydropersulfides and hydropolysulfides and also to prevent free thiols from attacking sulfur chains in hydropolysulfides and dialkyl polysulfides. However, recent findings reveal that alkylating agents can also destroy per- and polysulfides, to varying degrees, depending on the choice of alkylating agent. Here, we discuss the challenges associated with the alkylation of per- and polysulfides, the single most important step for their preservation and detection in biological samples.
    MeSH term(s) Humans ; Alkylating Agents ; Sulfides/chemistry ; Sulfur/chemistry ; Sulfhydryl Compounds
    Chemical Substances polysulfide (9080-49-3) ; Alkylating Agents ; Sulfides ; Sulfur (70FD1KFU70) ; Sulfhydryl Compounds
    Language English
    Publishing date 2023-07-18
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 1439176-4
    ISSN 1879-0402 ; 1367-5931
    ISSN (online) 1879-0402
    ISSN 1367-5931
    DOI 10.1016/j.cbpa.2023.102368
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Cystathionine β-synthase overexpression drives metastatic dissemination in pancreatic ductal adenocarcinoma via inducing epithelial-to-mesenchymal transformation of cancer cells.

    Czikora, Ágnes / Erdélyi, Katalin / Ditrói, Tamás / Szántó, Noémi / Jurányi, Eszter Petra / Szanyi, Szilárd / Tóvári, József / Strausz, Tamás / Nagy, Péter

    Redox biology

    2022  Volume 57, Page(s) 102505

    Abstract: Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest of all cancer types with a constant rise in global incidence. Therefore, better understanding of PDAC biology, in order to design more efficient diagnostic and treatment modalities, is a ... ...

    Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest of all cancer types with a constant rise in global incidence. Therefore, better understanding of PDAC biology, in order to design more efficient diagnostic and treatment modalities, is a priority. Here we found that the expression levels of cystathionine β-synthase (CBS), a transsulfuration enzyme, is markedly elevated in metastatic PDAC cells compared to cell lines isolated from non-metastatic primary tumors. On human immunohistochemical samples from PDAC patients we also found higher CBS staining in cancerous ductal cells compared to in non-tumor tissue, which was further elevated in the lymph node metastasis of the same patients. In mice, orthotopically injected CBS-silenced T3M4 cells induced fewer liver metastases compared to control cells indicating important roles for CBS in PDAC cancer cell invasion and malignant transformation. Wound healing and colony formation assays in cell culture confirmed that CBS-deficient metastatic T3M4 and non-metastatic BxPC3 primary tumor cells migrate slower and have impaired anchorage-independent growth capacities compared to control T3M4 cells. CBS silencing in T3M4 cells lowered WNT5a and SNAI1 gene expression down to levels that were observed in BxPC3 cells as well as resulted in an increase in E-cadherin and a decrease in Vimentin signals in mouse tumors when injected orthotopically. These observations suggested a primary role for the epithelial to mesenchymal transformation of cancer cells in CBS-mediated tumor aggressiveness. Under normal conditions, STAT3, an upstream regulator of Wnt signaling pathways, was less phosphorylated and more oxidized in shCBS T3M4 and BxPC3 compared to control T3M4 cells, which is consistent with decreased transcriptional activity at lower CBS levels due to less protection against oxidation. Sulfur metabolome analyses suggested that this CBS-mediated protection against oxidative modifications is likely to be related to persulfide/sulfide producing activities of the enzyme rather than its canonical function to produce cystathionine for cysteine synthesis. Taken together, CBS overexpression through regulation of the EMT plays a significant role in PDAC cancer cell invasion and metastasis.
    Language English
    Publishing date 2022-10-10
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2701011-9
    ISSN 2213-2317 ; 2213-2317
    ISSN (online) 2213-2317
    ISSN 2213-2317
    DOI 10.1016/j.redox.2022.102505
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Hydrogen sulfide as an anti-calcification stratagem in human aortic valve: Altered biogenesis and mitochondrial metabolism of H

    Combi, Zsolt / Potor, László / Nagy, Péter / Sikura, Katalin Éva / Ditrói, Tamás / Jurányi, Eszter Petra / Galambos, Klaudia / Szerafin, Tamás / Gergely, Péter / Whiteman, Matthew / Torregrossa, Roberta / Ding, Yuchao / Beke, Lívia / Hendrik, Zoltán / Méhes, Gábor / Balla, György / Balla, József

    Redox biology

    2023  Volume 60, Page(s) 102629

    Abstract: Hydrogen sulfide ( ... ...

    Abstract Hydrogen sulfide (H
    MeSH term(s) Humans ; Aortic Valve/metabolism ; Aortic Valve/pathology ; Aortic Valve Stenosis/metabolism ; Aortic Valve Stenosis/pathology ; Hydrogen Sulfide/metabolism ; Calcinosis/metabolism ; Calcinosis/pathology ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/metabolism ; Cells, Cultured ; Mitochondria/metabolism ; Mitochondrial Proteins/metabolism ; Nucleocytoplasmic Transport Proteins/metabolism
    Chemical Substances Hydrogen Sulfide (YY9FVM7NSN) ; Tumor Necrosis Factor-alpha ; ETHE1 protein, human ; Mitochondrial Proteins ; Nucleocytoplasmic Transport Proteins
    Language English
    Publishing date 2023-02-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2701011-9
    ISSN 2213-2317 ; 2213-2317
    ISSN (online) 2213-2317
    ISSN 2213-2317
    DOI 10.1016/j.redox.2023.102629
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The anti-inflammatory effect of dimethyl trisulfide in experimental acute pancreatitis.

    Orján, Erik Márk / Kormányos, Eszter Sára / Fűr, Gabriella Mihalekné / Dombi, Ágnes / Bálint, Emese Réka / Balla, Zsolt / Balog, Beáta Adél / Dágó, Ágnes / Totonji, Ahmad / Bátai, Zoárd István / Jurányi, Eszter Petra / Ditrói, Tamás / Al-Omari, Ammar / Pozsgai, Gábor / Kormos, Viktória / Nagy, Péter / Pintér, Erika / Rakonczay, Zoltán / Kiss, Lóránd

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 16813

    Abstract: Various organosulfur compounds, such as dimethyl trisulfide (DMTS), display anti-inflammatory properties. We aimed to examine the effects of DMTS on acute pancreatitis (AP) and its mechanism of action in both in vivo and in vitro studies. AP was induced ... ...

    Abstract Various organosulfur compounds, such as dimethyl trisulfide (DMTS), display anti-inflammatory properties. We aimed to examine the effects of DMTS on acute pancreatitis (AP) and its mechanism of action in both in vivo and in vitro studies. AP was induced in FVB/n mice or Wistar rats by caerulein, ethanol-palmitoleic acid, or L-ornithine-HCl. DMTS treatments were administered subcutaneously. AP severity was assessed by pancreatic histological scoring, pancreatic water content, and myeloperoxidase activity measurements. The behaviour of animals was followed. Pancreatic heat shock protein 72 (HSP72) expression, sulfide, and protein persulfidation were measured. In vitro acinar viability, intracellular Ca
    MeSH term(s) Rats ; Mice ; Animals ; Pancreatitis/pathology ; Rats, Wistar ; Acute Disease ; Pancreas/metabolism ; Sulfides/pharmacology ; Sulfides/therapeutic use ; Sulfides/metabolism ; Antioxidants/metabolism ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Anti-Inflammatory Agents/metabolism ; Ceruletide/pharmacology
    Chemical Substances dimethyl trisulfide (3E691T3NL1) ; Sulfides ; Antioxidants ; Anti-Inflammatory Agents ; Ceruletide (888Y08971B)
    Language English
    Publishing date 2023-10-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-43692-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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