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  1. Article: Editorial for the Special Issue: Diagnosis, Epidemiology and Transmission Dynamics of

    Köster, Pamela C / González-Barrio, David / Carmena, David

    Pathogens (Basel, Switzerland)

    2022  Volume 11, Issue 2

    Abstract: ... ...

    Abstract Cryptosporidium
    Language English
    Publishing date 2022-01-24
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens11020141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Cyst detection and viability assessment of

    García-Rodríguez, Juan José / Köster, Pamela C / Ponce-Gordo, Francisco

    Food and waterborne parasitology

    2022  Volume 26, Page(s) e00143

    Abstract: ... The ... ...

    Abstract The ciliate
    Language English
    Publishing date 2022-01-05
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2405-6766
    ISSN (online) 2405-6766
    DOI 10.1016/j.fawpar.2021.e00143
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  3. Article ; Online: Giardia duodenalis: Detection by Quantitative Real-Time PCR and Molecular Diversity.

    Dashti, Alejandro / Köster, Pamela C / Carmena, David

    Methods in molecular biology (Clifton, N.J.)

    2021  Volume 2369, Page(s) 83–97

    Abstract: Conventional microscopy is being progressively replaced by molecular (PCR and Sanger sequencing) methods for the first-line detection, identification, and genotyping of diarrhea-causing enteric parasites in modern clinical and research laboratories. ... ...

    Abstract Conventional microscopy is being progressively replaced by molecular (PCR and Sanger sequencing) methods for the first-line detection, identification, and genotyping of diarrhea-causing enteric parasites in modern clinical and research laboratories. These technologies allow increased sample testing, improved throughput, and optimized laboratory workflow in a cost-effective manner. Here, we describe the methodological algorithm used in a national reference center for the diagnosis and molecular characterization of Giardia duodenalis, a major contributor to the enormous burden of diarrheal disease globally. Initial detection of the parasite is conducted by a sensitive real-time PCR to amplify the small subunit of the ribosomal RNA of G. duodenalis, whereas a multilocus sequence genotyping scheme based on three constitutive genetic markers (glutamate dehydrogenase, β-giardin, and triose phosphate isomerase) is used for genotyping and sub-genotyping purposes. The advantages and disadvantages of these PCR-based methods are commented.
    MeSH term(s) Diarrhea ; Feces ; Genotype ; Giardia lamblia/genetics ; Giardiasis/diagnosis ; Glutamate Dehydrogenase/genetics ; Humans ; Multilocus Sequence Typing ; RNA, Ribosomal ; Real-Time Polymerase Chain Reaction
    Chemical Substances RNA, Ribosomal ; Glutamate Dehydrogenase (EC 1.4.1.2)
    Language English
    Publishing date 2021-07-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1681-9_6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Evaluation of the Use of Singleplex and Duplex CerTest VIASURE Real-Time PCR Assays to Detect Common Intestinal Protist Parasites.

    Dashti, Alejandro / Alonso, Henar / Escolar-Miñana, Cristina / Köster, Pamela C / Bailo, Begoña / Carmena, David / González-Barrio, David

    Diagnostics (Basel, Switzerland)

    2024  Volume 14, Issue 3

    Abstract: ... ...

    Abstract Cryptosporidium
    Language English
    Publishing date 2024-02-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics14030319
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  5. Article: Human-Borne Pathogens: Are They Threatening Wild Great Ape Populations?

    Köster, Pamela C. / Lapuente, Juan / Cruz, Israel / Carmena, David / Ponce-Gordo, Francisco

    Veterinary sciences. 2022 July 13, v. 9, no. 7

    2022  

    Abstract: Climate change and anthropic activities are the two main factors explaining wild great ape habitat reduction and population decline. The extent to which human-borne infectious diseases are contributing to this trend is still poorly understood. This is ... ...

    Abstract Climate change and anthropic activities are the two main factors explaining wild great ape habitat reduction and population decline. The extent to which human-borne infectious diseases are contributing to this trend is still poorly understood. This is due to insufficient or fragmented knowledge on the abundance and distribution of current wild great ape populations, the difficulty obtaining optimal biological samples for diagnostic testing, and the scarcity of pathogen typing data of sufficient quality. This review summarises current information on the most clinically relevant pathogens of viral, bacterial, parasitic, and fungal nature for which transmission from humans to wild great apes is suspected. After appraising the robustness of available epidemiological and/or molecular typing evidence, we attempt to categorise each pathogen according to its likelihood of truly being of human origin. We further discuss those agents for which anthroponotic transmission is more likely. These include two viral (Human Metapneumovirus and Respiratory Syncytial Virus), one bacterial (diarrhoeagenic Escherichia coli), and two parasitic (Cryptosporidium spp. and Giardia duodenalis) pathogens. Finally, we identify the main drawbacks impairing research on anthroponotic pathogen transmission in wild great apes and propose research lines that may contribute to bridging current knowledge gaps.
    Keywords Cryptosporidium ; Giardia lamblia ; Human metapneumovirus ; Pongidae ; Respiratory syncytial virus ; climate change ; disease transmission ; enterovirulent Escherichia coli group ; fungi ; habitats ; humans ; pathogens ; population dynamics
    Language English
    Dates of publication 2022-0713
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2768971-2
    ISSN 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci9070356
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Cyst detection and viability assessment of Balantioides coli in environmental samples: Current status and future needs

    García-Rodríguez, Juan José / Köster, Pamela C. / Ponce-Gordo, Francisco

    Food and Waterborne Parasitology. 2022 Mar., v. 26

    2022  

    Abstract: The ciliate Balantioides coli is a human enteric parasite that can cause life-threatening infections. It is a food- and waterborne parasite, with cysts being the infective stage. Despite its importance as a potential pathogen, few reports have ... ...

    Abstract The ciliate Balantioides coli is a human enteric parasite that can cause life-threatening infections. It is a food- and waterborne parasite, with cysts being the infective stage. Despite its importance as a potential pathogen, few reports have investigated its presence in environmental samples, and some issues need attention including i) The accuracy of B. coli identification. In most cases, the protozoa is identified only by its morphological traits, which can be identical to those from other parasitic ciliates of animals. Genetic analysis of cysts recovered from environmental samples is necessary for species confirmation. In addition, genetic methods used with faecal samples need to be adequately validated with environmental matrices. ii) The methodology for searching this parasite in environmental samples. The protocols include an initial phase to isolate the cysts from the matrix followed by a second phase in which concentration procedures are usually applied. The methods may be valid but are not standardised and differences between studies could affect the results obtained. iii) The areas that needs further research. The development of genetic identification methods and standardised analytical protocols in environmental samples are required, as well as the assessment of viability and infectivity of B. coli cysts. The development of axenic culture systems will boost research on this parasite.
    Keywords Balantioides coli ; Protozoa ; axenic culture ; genetic analysis ; genetic testing ; humans ; parasites ; parasitology ; pathogenicity ; pathogens ; viability
    Language English
    Dates of publication 2022-03
    Publishing place Elsevier Inc.
    Document type Article
    ISSN 2405-6766
    DOI 10.1016/j.fawpar.2021.e00143
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Cryptosporidium hominis infections in non-human animal species: revisiting the concept of host specificity.

    Widmer, Giovanni / Köster, Pamela C / Carmena, David

    International journal for parasitology

    2020  Volume 50, Issue 4, Page(s) 253–262

    Abstract: Parasites in the genus Cryptosporidium, phylum Apicomplexa, are found worldwide in the intestinal tract of many vertebrate species and in the environment. Driven by sensitive PCR methods, and the availability of abundant sequence data and reference ... ...

    Abstract Parasites in the genus Cryptosporidium, phylum Apicomplexa, are found worldwide in the intestinal tract of many vertebrate species and in the environment. Driven by sensitive PCR methods, and the availability of abundant sequence data and reference genomes, the taxonomic complexity of the genus has steadily increased; 38 species have been named to date. Due to its public health importance, Cryptosporidium hominis has long attracted the interest of the research community. This species was initially described as infectious to humans only. This perception has persisted in spite of an increasing number of observations of natural and experimental infections of animals with this species. Here we summarize and discuss this literature published since 2000 and conclude that the host range of C. hominis is broader than originally described. The evolving definition of the C. hominis host range raises interesting questions about host specificity and the evolution of Cryptosporidium parasites.
    MeSH term(s) Animals ; Animals, Wild/parasitology ; Cryptosporidiosis/parasitology ; Cryptosporidiosis/transmission ; Cryptosporidium/classification ; Cryptosporidium/genetics ; Cryptosporidium/isolation & purification ; Cryptosporidium parvum/classification ; Cryptosporidium parvum/genetics ; Cryptosporidium parvum/isolation & purification ; DNA, Protozoan/analysis ; Disease Reservoirs ; Genes, Protozoan ; Host Specificity/genetics ; Humans ; Livestock/parasitology ; Phylogeny ; Zoonoses
    Chemical Substances DNA, Protozoan
    Language English
    Publishing date 2020-03-20
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 120518-3
    ISSN 1879-0135 ; 0020-7519
    ISSN (online) 1879-0135
    ISSN 0020-7519
    DOI 10.1016/j.ijpara.2020.01.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 789: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Diagnóstico molecular de parasitosis intestinales.

    Dacal, Elena / Köster, Pamela C / Carmena, David

    Enfermedades infecciosas y microbiologia clinica (English ed.)

    2020  Volume 38 Suppl 1, Page(s) 24–31

    Abstract: Infections causes by parasites of the gastrointestinal tract are a global public health problem. In industrialised countries, their particular epidemiological (low general prevalence of enteroparasites), economic (high labour costs) and clinical ... ...

    Abstract Infections causes by parasites of the gastrointestinal tract are a global public health problem. In industrialised countries, their particular epidemiological (low general prevalence of enteroparasites), economic (high labour costs) and clinical characteristics (constant increase in the number of samples and diagnostic determinations to be performed) have led molecular techniques to progressively replace conventional microscopy as the first-line diagnostic method of these pathogens in modern clinical laboratories. PCR-based techniques, particularly those developed for the simultaneous detection of the various agents that can cause the same infectious disease (syndromic diagnosis), already represent a cost-effective option that allow process automisation, workflow optimisation, and comparison of results among different laboratories, and facilitate accreditation of diagnostic procedures. This review clearly and concisely discusses the current situation of the molecular diagnosis of the main species of intestinal parasites in humans, particularly the enteric protozoans causing diarrhoea (Cryptosporidium spp., Giardia duodenalis, Entamoeba histolytica), the most important members the Microsporidia phyla (Enterocytozoon bieneusi) and Stramenopiles phyla (Blastocystis sp.), as well as the helminths transmitted by soil (Ancylostoma spp., Ascaris lumbricoides, Necator americanus, Strongyloides stercoralis and Trichuris trichiura) and food (Anisakis spp., Clonorchis sinensis, Fasciola spp., Taenia solium, and Trichinella spiralis). Special attention is paid to the description of available techniques and formats, to their diagnostic benefits and the most widely used genetic markers for their detection, both in clinical laboratories and genotyping in referral and research centres.
    MeSH term(s) Animals ; Diarrhea/parasitology ; Feces ; Helminthiasis/diagnosis ; Humans ; Intestinal Diseases, Parasitic/diagnosis ; Molecular Diagnostic Techniques ; Protozoan Infections/diagnosis ; Protozoan Infections/parasitology ; Real-Time Polymerase Chain Reaction
    Language Spanish
    Publishing date 2020-02-28
    Document type Journal Article ; Review
    ISSN 2529-993X
    ISSN (online) 2529-993X
    DOI 10.1016/j.eimc.2020.02.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Cryptosporidium hominis infections in non-human animal species: revisiting the concept of host specificity

    Widmer, Giovanni / Köster, Pamela C / Carmena, David

    Australian Society for Parasitology International journal for parasitology. 2020 Apr., v. 50, no. 4

    2020  

    Abstract: Parasites in the genus Cryptosporidium, phylum Apicomplexa, are found worldwide in the intestinal tract of many vertebrate species and in the environment. Driven by sensitive PCR methods, and the availability of abundant sequence data and reference ... ...

    Abstract Parasites in the genus Cryptosporidium, phylum Apicomplexa, are found worldwide in the intestinal tract of many vertebrate species and in the environment. Driven by sensitive PCR methods, and the availability of abundant sequence data and reference genomes, the taxonomic complexity of the genus has steadily increased; 38 species have been named to date. Due to its public health importance, Cryptosporidium hominis has long attracted the interest of the research community. This species was initially described as infectious to humans only. This perception has persisted in spite of an increasing number of observations of natural and experimental infections of animals with this species. Here we summarize and discuss this literature published since 2000 and conclude that the host range of C. hominis is broader than originally described. The evolving definition of the C. hominis host range raises interesting questions about host specificity and the evolution of Cryptosporidium parasites.
    Keywords Cryptosporidium hominis ; animal diseases ; evolution ; genome ; host range ; host specificity ; intestines ; parasites ; polymerase chain reaction ; public health
    Language English
    Dates of publication 2020-04
    Size p. 253-262.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 120518-3
    ISSN 1879-0135 ; 0020-7519
    ISSN (online) 1879-0135
    ISSN 0020-7519
    DOI 10.1016/j.ijpara.2020.01.005
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 789: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: Human-Borne Pathogens: Are They Threatening Wild Great Ape Populations?

    Köster, Pamela C / Lapuente, Juan / Cruz, Israel / Carmena, David / Ponce-Gordo, Francisco

    Veterinary sciences

    2022  Volume 9, Issue 7

    Abstract: Climate change and anthropic activities are the two main factors explaining wild great ape habitat reduction and population decline. The extent to which human-borne infectious diseases are contributing to this trend is still poorly understood. This is ... ...

    Abstract Climate change and anthropic activities are the two main factors explaining wild great ape habitat reduction and population decline. The extent to which human-borne infectious diseases are contributing to this trend is still poorly understood. This is due to insufficient or fragmented knowledge on the abundance and distribution of current wild great ape populations, the difficulty obtaining optimal biological samples for diagnostic testing, and the scarcity of pathogen typing data of sufficient quality. This review summarises current information on the most clinically relevant pathogens of viral, bacterial, parasitic, and fungal nature for which transmission from humans to wild great apes is suspected. After appraising the robustness of available epidemiological and/or molecular typing evidence, we attempt to categorise each pathogen according to its likelihood of truly being of human origin. We further discuss those agents for which anthroponotic transmission is more likely. These include two viral (Human Metapneumovirus and Respiratory Syncytial Virus), one bacterial (diarrhoeagenic
    Language English
    Publishing date 2022-07-13
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2768971-2
    ISSN 2306-7381 ; 2306-7381
    ISSN (online) 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci9070356
    Database MEDical Literature Analysis and Retrieval System OnLINE

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