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  1. Article: Pull-Down Into Active Inclusion Bodies and Their Application in the Detection of (Poly)-Phosphates and Metal-Ions.

    Hrabarova, Eva / Belkova, Martina / Koszagova, Romana / Nahalka, Jozef

    Frontiers in bioengineering and biotechnology

    2022  Volume 10, Page(s) 833192

    Abstract: Inclusion bodies are typically ignored as they are considered unwanted protein waste generated by prokaryotic host cells during recombinant protein production or harmful protein inclusions in human cell biology. However, these protein particles may have ... ...

    Abstract Inclusion bodies are typically ignored as they are considered unwanted protein waste generated by prokaryotic host cells during recombinant protein production or harmful protein inclusions in human cell biology. However, these protein particles may have applications for
    Language English
    Publishing date 2022-03-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2022.833192
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts.

    Köszagová, Romana / Hrabárová, Eva / Achbergerová, Lucia / Nahálka, Jozef

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2406, Page(s) 501–515

    Abstract: Biocatalysis and biotransformations have a broad application in industrial synthetic chemistry. In addition to the whole cell catalysis, purified recombinant enzymes are successfully used for biocatalysis of specific chemical reactions. In this ... ...

    Abstract Biocatalysis and biotransformations have a broad application in industrial synthetic chemistry. In addition to the whole cell catalysis, purified recombinant enzymes are successfully used for biocatalysis of specific chemical reactions. In this contribution, we report characterization, immobilization, and application of several model target enzymes (D-amino acid oxidase, sialic acid aldolase, maltodextrin phosphorylase, polyphosphate kinase, UDP-glucose pyrophosphorylase) physiologically aggregated within inclusion bodies retaining their biological activity as immobilized biocatalysts.
    MeSH term(s) Bacteria/chemistry ; Bacteria/metabolism ; Biocatalysis ; Biotransformation ; Enzymes, Immobilized/chemistry ; Inclusion Bodies
    Chemical Substances Enzymes, Immobilized
    Language English
    Publishing date 2022-01-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1859-2_30
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Magnetization of active inclusion bodies: comparison with centrifugation in repetitive biotransformations.

    Koszagova, Romana / Krajcovic, Tomas / Palencarova-Talafova, Klaudia / Patoprsty, Vladimir / Vikartovska, Alica / Pospiskova, Kristyna / Safarik, Ivo / Nahalka, Jozef

    Microbial cell factories

    2018  Volume 17, Issue 1, Page(s) 139

    Abstract: Background: Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle " ... ...

    Abstract Background: Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction cycle to another, as high centrifugation forces are needed in large volumes. The magnetization of inclusion bodies is a smart solution for large-scale applications, enabling an easier separation process using a magnetic field.
    Results: Magnetically modified inclusion bodies of UDP-glucose pyrophosphorylase were recycled 50 times, in comparison, inclusion bodies of the same enzyme were inactivated during ten reaction cycles if they were recycled by centrifugation. Inclusion bodies of sialic acid aldolase also showed good performance and operational stability after the magnetization procedure.
    Conclusions: It is demonstrated here that inclusion bodies can be easily magnetically modified by magnetic iron oxide particles prepared by microwave-assisted synthesis from ferrous sulphate. The magnetic particles stabilize the repetitive use of the inclusion bodies .
    MeSH term(s) Biotransformation/physiology ; Centrifugation/methods ; Inclusion Bodies/metabolism
    Language English
    Publishing date 2018-09-03
    Publishing country England
    Document type Journal Article
    ISSN 1475-2859
    ISSN (online) 1475-2859
    DOI 10.1186/s12934-018-0987-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

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