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  1. Article: Optimization of process parameters for continuous kheer-making machine

    Kadam, Swati / Ashis K. Datta / Tushar Gulati

    Lebensmittel-Wissenschaft + [i.e. und] Technologie. 2013 Apr., v. 51, no. 1

    2013  

    Abstract: If rice is cooked in milk, starch–milk reaction results into a thick product, which is very popular in India known as kheer. Conventionally, kheer is prepared by cooking rice in milk in an open pan over low fire followed by addition of sugar toward the ... ...

    Abstract If rice is cooked in milk, starch–milk reaction results into a thick product, which is very popular in India known as kheer. Conventionally, kheer is prepared by cooking rice in milk in an open pan over low fire followed by addition of sugar toward the end. The present investigation aims to optimize the process parameters (operating pressure and cooking time) for designing the pressurized cooking section of a continuous kheer-making machine. Sensory trials of kheer prepared conventionally and using pressurized methods were carried out and the data was analyzed using Fuzzy Logic. Sensory results of open-pan samples indicated that there is a small range of Whiteness Index (WI) and Hardness (H) values that is desirable in kheer. An Artificial Neural Network-Genetic Algorithm (ANN-GA) model was developed to further optimize the operating parameters to result in a product that would have the desired color and texture observed in kheer prepared conventionally. The developed ANN-GA model was successful in providing with input conditions leading to desired WI and H values. Finally, from the set of optimal input conditions, operating pressure of 0.27 MPa and cooking time of 7.5 min was chosen for designing the pressurized cooking section of the continuous kheer-making machine.
    Keywords algorithms ; color ; fuzzy logic ; hardness ; milk ; pressure cooking ; rice ; rice milk ; sugars ; texture ; India
    Language English
    Dates of publication 2013-04
    Size p. 94-103.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 241369-3
    ISSN 0460-1173 ; 0023-6438
    ISSN 0460-1173 ; 0023-6438
    DOI 10.1016/j.lwt.2012.10.002
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2634: Show issues

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  2. Article: 5-hydroxymethylcytosine and its potential roles in development and cancer.

    Pfeifer, Gerd P / Kadam, Swati / Jin, Seung-Gi

    Epigenetics & chromatin

    2013  Volume 6, Issue 1, Page(s) 10

    Abstract: Only a few years ago it was demonstrated that mammalian DNA contains oxidized forms of 5-methylcytosine (5mC). The base 5-hydroxymethylcytosine (5hmC) is the most abundant of these oxidation products and is referred to as the sixth DNA base. 5hmC is ... ...

    Abstract Only a few years ago it was demonstrated that mammalian DNA contains oxidized forms of 5-methylcytosine (5mC). The base 5-hydroxymethylcytosine (5hmC) is the most abundant of these oxidation products and is referred to as the sixth DNA base. 5hmC is produced from 5mC in an enzymatic pathway involving three 5mC oxidases, Ten-eleven translocation (TET)1, TET2, and TET3. The biological role of 5hmC is still unclear. Current models propose that 5hmC is an intermediate base in an active or passive DNA demethylation process that operates during important reprogramming phases of mammalian development. Tumors originating in various human tissues have strongly depleted levels of 5hmC. Apparently, 5hmC cannot be maintained in proliferating cells. Furthermore, mutations in the TET2 gene are commonly observed in human myeloid malignancies. Since TET proteins and many lysine demethylases require 2-oxoglutarate as a cofactor, aberrations in cofactor biochemical pathways, including mutations in isocitrate dehydrogenase (IDH), may affect levels of 5hmC and 5mC in certain types of tumors, either directly or indirectly. We discuss current data and models of the function of 5hmC in general, with special emphasis on its role in mechanisms of development and cancer.
    Language English
    Publishing date 2013-05-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2462129-8
    ISSN 1756-8935
    ISSN 1756-8935
    DOI 10.1186/1756-8935-6-10
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine

    Jin, Seung-Gi / Kadam, Swati / Pfeifer, Gerd P

    Nucleic acids research. 2010 June, v. 38, no. 11

    2010  

    Abstract: DNA cytosine-5 methylation is a well-studied epigenetic pathway implicated in gene expression control and disease pathogenesis. Different technologies have been developed to examine the distribution of 5-methylcytosine (5mC) in specific sequences of the ... ...

    Abstract DNA cytosine-5 methylation is a well-studied epigenetic pathway implicated in gene expression control and disease pathogenesis. Different technologies have been developed to examine the distribution of 5-methylcytosine (5mC) in specific sequences of the genome. Recently, substantial amounts of 5-hydroxymethylcytosine (5hmC), most likely derived from enzymatic oxidation of 5mC by TET1, have been detected in certain mammalian tissues. Here, we have examined the ability of several commonly used DNA methylation profiling methods to distinguish between 5mC and 5hmC. We show that techniques based on sodium bisulfite treatment of DNA are incapable of distinguishing between the two modified bases. In contrast, techniques based on immunoprecipitation with anti-5mC antibody (methylated DNA immunoprecipitation, MeDIP) or those based on proteins that bind to methylated CpG sequences (e.g. methylated-CpG island recovery assay, MIRA) do not detect 5hmC and are specific for 5mC unless both modified bases occur in the same DNA fragment. We also report that several methyl-CpG binding proteins including MBD1, MBD2 and MBD4 do not bind to sequences containing 5hmC. Selective mapping of 5hmC will require the development of unique tools for the detection of this modified base.
    Keywords DNA ; DNA fragmentation ; DNA methylation ; antibodies ; binding proteins ; epigenetics ; gene expression ; genome ; mammals ; oxidation ; pathogenesis ; precipitin tests ; sodium bisulfite ; tissues
    Language English
    Dates of publication 2010-06
    Size p. e125.
    Document type Article
    ZDB-ID 186809-3
    ISSN 0301-5610 ; 0305-1048
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkq223
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 79/704: Show issues

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  4. Article ; Online: MIRA-seq for DNA methylation analysis of CpG islands.

    Jung, Marc / Kadam, Swati / Xiong, Wenying / Rauch, Tibor A / Jin, Seung-Gi / Pfeifer, Gerd P

    Epigenomics

    2015  Volume 7, Issue 5, Page(s) 695–706

    Abstract: Aim: To develop a reliable method for whole genome analysis of DNA methylation.: Materials & methods: Genome-scale analysis of DNA methylation includes affinity-based approaches such as enrichment using methyl-CpG-binding proteins. One of these ... ...

    Abstract Aim: To develop a reliable method for whole genome analysis of DNA methylation.
    Materials & methods: Genome-scale analysis of DNA methylation includes affinity-based approaches such as enrichment using methyl-CpG-binding proteins. One of these methods, the methylated-CpG island recovery assay (MIRA), is based on the high affinity of the MBD2b-MBD3L1 complex for CpG-methylated DNA. Here we provide a detailed description of MIRA and combine it with next generation sequencing platforms (MIRA-seq).
    Results: We assessed the performance of MIRA-seq and compared the data with whole genome bisulfite sequencing.
    Conclusion: MIRA-seq is a reliable, genome-scale DNA methylation analysis platform for scoring DNA methylation differences at CpG-rich genomic regions. The method is not limited by primer or probe design and is cost effective.
    MeSH term(s) Cell Line ; CpG Islands/genetics ; DNA Methylation ; Epigenomics/methods ; Fibroblasts/cytology ; Fibroblasts/metabolism ; Genome, Human/genetics ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Models, Genetic ; Reproducibility of Results
    Language English
    Publishing date 2015-08
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1750-192X
    ISSN (online) 1750-192X
    DOI 10.2217/epi.15.33
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine.

    Jin, Seung-Gi / Kadam, Swati / Pfeifer, Gerd P

    Nucleic acids research

    2010  Volume 38, Issue 11, Page(s) e125

    Abstract: DNA cytosine-5 methylation is a well-studied epigenetic pathway implicated in gene expression control and disease pathogenesis. Different technologies have been developed to examine the distribution of 5-methylcytosine (5mC) in specific sequences of the ... ...

    Abstract DNA cytosine-5 methylation is a well-studied epigenetic pathway implicated in gene expression control and disease pathogenesis. Different technologies have been developed to examine the distribution of 5-methylcytosine (5mC) in specific sequences of the genome. Recently, substantial amounts of 5-hydroxymethylcytosine (5hmC), most likely derived from enzymatic oxidation of 5mC by TET1, have been detected in certain mammalian tissues. Here, we have examined the ability of several commonly used DNA methylation profiling methods to distinguish between 5mC and 5hmC. We show that techniques based on sodium bisulfite treatment of DNA are incapable of distinguishing between the two modified bases. In contrast, techniques based on immunoprecipitation with anti-5mC antibody (methylated DNA immunoprecipitation, MeDIP) or those based on proteins that bind to methylated CpG sequences (e.g. methylated-CpG island recovery assay, MIRA) do not detect 5hmC and are specific for 5mC unless both modified bases occur in the same DNA fragment. We also report that several methyl-CpG binding proteins including MBD1, MBD2 and MBD4 do not bind to sequences containing 5hmC. Selective mapping of 5hmC will require the development of unique tools for the detection of this modified base.
    MeSH term(s) 5-Methylcytosine/analysis ; 5-Methylcytosine/immunology ; Antibodies ; Cytosine/analogs & derivatives ; Cytosine/analysis ; Cytosine/metabolism ; DNA/isolation & purification ; DNA Methylation ; DNA-Binding Proteins/metabolism ; Electrophoretic Mobility Shift Assay ; Immunoprecipitation ; Oligonucleotides/chemistry ; Sequence Analysis, DNA ; Sulfites
    Chemical Substances Antibodies ; DNA-Binding Proteins ; Oligonucleotides ; Sulfites ; 5-hydroxymethylcytosine (1123-95-1) ; 5-Methylcytosine (6R795CQT4H) ; Cytosine (8J337D1HZY) ; DNA (9007-49-2) ; sodium bisulfite (TZX5469Z6I)
    Language English
    Publishing date 2010-04-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkq223
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: TGF-β induces acetylation of chromatin and of Ets-1 to alleviate repression of miR-192 in diabetic nephropathy.

    Kato, Mitsuo / Dang, Varun / Wang, Mei / Park, Jung Tak / Deshpande, Supriya / Kadam, Swati / Mardiros, Armen / Zhan, Yumei / Oettgen, Peter / Putta, Sumanth / Yuan, Hang / Lanting, Linda / Natarajan, Rama

    Science signaling

    2013  Volume 6, Issue 278, Page(s) ra43

    Abstract: MicroRNAs (miRNAs), such as miR-192, mediate the actions of transforming growth factor-β1 (TGF-β) related to the pathogenesis of diabetic kidney diseases. We found that the biphasic induction of miR-192 expression by TGF-β in mouse renal glomerular ... ...

    Abstract MicroRNAs (miRNAs), such as miR-192, mediate the actions of transforming growth factor-β1 (TGF-β) related to the pathogenesis of diabetic kidney diseases. We found that the biphasic induction of miR-192 expression by TGF-β in mouse renal glomerular mesangial cells initially involved the Smad transcription factors, followed by sustained expression that was promoted by acetylation of the transcription factor Ets-1 and of histone H3 by the acetyltransferase p300, which was activated by the serine and threonine kinase Akt. In mesangial cells from Ets-1-deficient mice or in cells in which Ets-1 was knocked down, basal amounts of miR-192 were higher than those in control cells, but sustained induction of miR-192 by TGF-β was attenuated. Furthermore, inhibition of Akt or ectopic expression of dominant-negative histone acetyltransferases decreased p300-mediated acetylation and Ets-1 dissociation from the miR-192 promoter and prevented miR-192 expression in response to TGF-β. Activation of Akt and p300 and acetylation of Ets-1 and histone H3 were increased in glomeruli from diabetic db/db mice compared to nondiabetic db/+ mice, suggesting that this pathway may contribute to diabetic nephropathy. These findings provide insight into the regulation of miRNAs through signaling-mediated changes in transcription factor activity and in epigenetic histone acetylation under normal and disease states.
    MeSH term(s) Acetylation ; Chromatin/physiology ; Diabetic Nephropathies/physiopathology ; Humans ; MicroRNAs/genetics ; MicroRNAs/physiology ; Transcription Factors/metabolism ; Transforming Growth Factor beta/physiology
    Chemical Substances Chromatin ; MIRN92 microRNA, human ; MicroRNAs ; Transcription Factors ; Transforming Growth Factor beta
    Language English
    Publishing date 2013-06-04
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2417226-1
    ISSN 1937-9145 ; 1945-0877
    ISSN (online) 1937-9145
    ISSN 1945-0877
    DOI 10.1126/scisignal.2003389
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Optimization of process parameters for continuous kheer-making machine

    Kadam, Swati / Gulati, Tushar / Datta, Ashis K.

    Lebensmittel-Wissenschaft + [i.e. und] Technologie

    Volume v. 51,, Issue no. 1

    Abstract: If rice is cooked in milk, starch–milk reaction results into a thick product, which is very popular in India known as kheer. Conventionally, kheer is prepared by cooking rice in milk in an open pan over low fire followed by addition of sugar toward the ... ...

    Abstract If rice is cooked in milk, starch–milk reaction results into a thick product, which is very popular in India known as kheer. Conventionally, kheer is prepared by cooking rice in milk in an open pan over low fire followed by addition of sugar toward the end. The present investigation aims to optimize the process parameters (operating pressure and cooking time) for designing the pressurized cooking section of a continuous kheer-making machine. Sensory trials of kheer prepared conventionally and using pressurized methods were carried out and the data was analyzed using Fuzzy Logic. Sensory results of open-pan samples indicated that there is a small range of Whiteness Index (WI) and Hardness (H) values that is desirable in kheer. An Artificial Neural Network-Genetic Algorithm (ANN-GA) model was developed to further optimize the operating parameters to result in a product that would have the desired color and texture observed in kheer prepared conventionally. The developed ANN-GA model was successful in providing with input conditions leading to desired WI and H values. Finally, from the set of optimal input conditions, operating pressure of 0.27 MPa and cooking time of 7.5 min was chosen for designing the pressurized cooking section of the continuous kheer-making machine.
    Keywords pressure cooking ; fuzzy logic ; rice ; color ; texture ; milk ; rice milk ; algorithms ; hardness ; sugars
    Language English
    Document type Article
    ISSN 0023-6438
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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