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  1. Article ; Online: A simple sexing test for elephant species and its application to faecal DNA.

    Bourgeois, Stéphanie / Ouitavon, K / Kongmee, P / Veeramaethaphan, T / Kaden, J / McEwing, R

    Journal of applied genetics

    2021  Volume 62, Issue 3, Page(s) 507–509

    Abstract: We developed a novel real-time PCR assay for rapid sexing in all three elephant species, which amplifies small fragments of the orthologous sexual chromosome zinc finger protein genes ZFX/ZFY (65 bp). This assay is a simple, inexpensive and reliable tool ...

    Abstract We developed a novel real-time PCR assay for rapid sexing in all three elephant species, which amplifies small fragments of the orthologous sexual chromosome zinc finger protein genes ZFX/ZFY (65 bp). This assay is a simple, inexpensive and reliable tool that is suitable for non-invasive DNA samples and can be incorporated into larger SNP panels for individual identification and population genetic studies.
    MeSH term(s) Animals ; DNA ; DNA-Binding Proteins/genetics ; Elephants/genetics ; Feces ; Female ; Male ; Real-Time Polymerase Chain Reaction/veterinary ; Sex Determination Analysis ; Zinc Fingers
    Chemical Substances DNA-Binding Proteins ; DNA (9007-49-2)
    Language English
    Publishing date 2021-03-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1235302-4
    ISSN 2190-3883 ; 1234-1983
    ISSN (online) 2190-3883
    ISSN 1234-1983
    DOI 10.1007/s13353-021-00627-2
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  2. Article: IL-6 determination in serum of kidney graft recipients by a new bedside test: its diagnostic relevance.

    Kaden, J

    Transplantation proceedings

    2007  Volume 39, Issue 2, Page(s) 511–513

    Abstract: Interleukin-6 (IL-6) has diagnostic importance as one of the earliest indicators of inflammation, tissue damage, hypoxia, or infection. In contrast to the time-consuming immunoassays actually used in many laboratories, results of a new IL-6 bedside test, ...

    Abstract Interleukin-6 (IL-6) has diagnostic importance as one of the earliest indicators of inflammation, tissue damage, hypoxia, or infection. In contrast to the time-consuming immunoassays actually used in many laboratories, results of a new IL-6 bedside test, which is interpreted visually or photometrically, are available at 20 minutes. The photometric measurement using a stored standard curve allowed differentiation of five ranges: <50, 50-100, >100-200, >200-500, and >500-1000 pg/mL. The aim of our study was to evaluate the diagnostic relevance of this new IL-6 bedside test in four well-defined, noninfectious and infectious events after kidney transplantation. In a retrospective study the IL-6 serum levels were measured in a total of 269 serum samples from 27 renal graft recipients. Analysis of all data clearly showed that the majority of elevated IL-6 levels occurred in connection with bacterial infections. Viral infections did not result in an increase of IL-6 >50 pg/mL. Thus, from a clinical point of view this new bedside test can be recommended.
    MeSH term(s) Adult ; Bacterial Infections/diagnosis ; Biomarkers/blood ; Cadaver ; Female ; Humans ; Interleukin-6/blood ; Kidney Transplantation/physiology ; Male ; Middle Aged ; Point-of-Care Systems ; Postoperative Complications/diagnosis ; Postoperative Complications/microbiology ; Tissue Donors ; Virus Diseases/diagnosis
    Chemical Substances Biomarkers ; Interleukin-6
    Language English
    Publishing date 2007-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 82046-5
    ISSN 1873-2623 ; 0041-1345
    ISSN (online) 1873-2623
    ISSN 0041-1345
    DOI 10.1016/j.transproceed.2006.12.015
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  3. Article ; Online: Do badgers eat lamb? Using DNA and post-mortem analysis to investigate lamb predation on Scottish farms

    George, Sheila / Campbell, Steve / Kaden, Jennifer / Hale, Catherine / Webster, Lucy

    2023  

    Keywords Text ; abstract_or_summary ; ddc:630
    Language English
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Book ; Thesis: Wirkung des spezifischen Herzfrequenzsenkers Zatebradine auf Hämodynamik und linksventrikuläres Remodeling nach experimentellem Myokardinfarkt bei Ratten

    Kaden, Jens

    1996  

    Author's details vorgelegt von Jens Kaden
    Language German
    Size 64 Bl. : graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Heidelberg, Univ., Diss., 1997
    HBZ-ID HT008165288
    Database Catalogue ZB MED Medicine, Health

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  5. Article: After wounding, a G-protein coupled receptor promotes the restoration of tension in epithelial cells.

    Han, Ivy / Hua, Junmin / White, James S / O'Connor, James T / Nassar, Lila S / Tro, Kaden J / Page-McCaw, Andrea / Hutson, M Shane

    bioRxiv : the preprint server for biology

    2024  

    Abstract: The maintenance of epithelial barrier function involves cellular tension, with cells pulling on their neighbors to maintain epithelial integrity. Wounding interrupts cellular tension, which may serve as an early signal to initiate epithelial repair. To ... ...

    Abstract The maintenance of epithelial barrier function involves cellular tension, with cells pulling on their neighbors to maintain epithelial integrity. Wounding interrupts cellular tension, which may serve as an early signal to initiate epithelial repair. To characterize how wounds alter cellular tension, we used a laser-recoil assay to map cortical tension around wounds in the epithelial monolayer of the
    Language English
    Publishing date 2024-02-18
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.05.31.543122
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: After wounding, a G-protein coupled receptor promotes the restoration of tension in epithelial cells.

    Han, Ivy S / Hua, Junmin / White, James S / O'Connor, James T / Nassar, Lila S / Tro, Kaden J / Page-McCaw, Andrea / Hutson, M Shane

    Molecular biology of the cell

    2024  Volume 35, Issue 5, Page(s) ar66

    Abstract: The maintenance of epithelial barrier function involves cellular tension, with cells pulling on their neighbors to maintain epithelial integrity. Wounding interrupts cellular tension, which may serve as an early signal to initiate epithelial repair. To ... ...

    Abstract The maintenance of epithelial barrier function involves cellular tension, with cells pulling on their neighbors to maintain epithelial integrity. Wounding interrupts cellular tension, which may serve as an early signal to initiate epithelial repair. To characterize how wounds alter cellular tension we used a laser-recoil assay to map cortical tension around wounds in the epithelial monolayer of the
    MeSH term(s) Animals ; Wound Healing/physiology ; Epithelial Cells/physiology ; Receptors, G-Protein-Coupled ; Signal Transduction ; Drosophila
    Chemical Substances Receptors, G-Protein-Coupled
    Language English
    Publishing date 2024-03-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1098979-1
    ISSN 1939-4586 ; 1059-1524
    ISSN (online) 1939-4586
    ISSN 1059-1524
    DOI 10.1091/mbc.E23-05-0204
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  7. Article ; Online: Disruption of IL-17-mediated immunosurveillance in the respiratory mucosa results in invasive

    Mills, Jamie-Lee / Lepletier, Ailin / Ozberk, Victoria / Dooley, Jessica / Kaden, Jacqualine / Calcutt, Ainslie / Huo, Yongbao / Hicks, Allan / Zaid, Ali / Good, Michael F / Pandey, Manisha

    Frontiers in immunology

    2024  Volume 15, Page(s) 1351777

    Abstract: Introduction: Streptococcus pyogenes: Methods: Here, we modeled single or repeated non-lethal intranasal (IN) : Results: Immunocompetent mice that received a single : Discussion: Our results demonstrated that IL-17 plays a critical role in ... ...

    Abstract Introduction: Streptococcus pyogenes
    Methods: Here, we modeled single or repeated non-lethal intranasal (IN)
    Results: Immunocompetent mice that received a single
    Discussion: Our results demonstrated that IL-17 plays a critical role in preventing invasive disease following
    MeSH term(s) Animals ; Mice ; Interleukin-17 ; Monitoring, Immunologic ; Respiratory Mucosa ; Streptococcal Infections ; Streptococcus pyogenes
    Chemical Substances Interleukin-17 ; Il17a protein, mouse
    Language English
    Publishing date 2024-03-21
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2024.1351777
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  8. Article: A simple sexing test for elephant species and its application to faecal DNA

    Bourgeois, Stéphanie / Ouitavon, K. / Kongmee, P. / Veeramaethaphan, T. / Kaden, J. / McEwing, R.

    Journal of applied genetics. 2021 Sept., v. 62, no. 3

    2021  

    Abstract: We developed a novel real-time PCR assay for rapid sexing in all three elephant species, which amplifies small fragments of the orthologous sexual chromosome zinc finger protein genes ZFX/ZFY (65 bp). This assay is a simple, inexpensive and reliable tool ...

    Abstract We developed a novel real-time PCR assay for rapid sexing in all three elephant species, which amplifies small fragments of the orthologous sexual chromosome zinc finger protein genes ZFX/ZFY (65 bp). This assay is a simple, inexpensive and reliable tool that is suitable for non-invasive DNA samples and can be incorporated into larger SNP panels for individual identification and population genetic studies.
    Keywords DNA ; chromosomes ; quantitative polymerase chain reaction ; zinc finger motif
    Language English
    Dates of publication 2021-09
    Size p. 507-509.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 1235302-4
    ISSN 2190-3883 ; 1234-1983
    ISSN (online) 2190-3883
    ISSN 1234-1983
    DOI 10.1007/s13353-021-00627-2
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Association between students' personality traits and hand hygiene compliance during objective standardized clinical examinations.

    Schüttpelz-Brauns, K / Obertacke, U / Kaden, J / Hagl, C I

    The Journal of hospital infection

    2015  Volume 89, Issue 3, Page(s) 210–214

    Abstract: Background: Although the need for hand hygiene (HH) is generally accepted, studies continue to document inadequate compliance. Medical students are taught about the importance of HH to prevent nosocomial infections, and receive training in the correct ... ...

    Abstract Background: Although the need for hand hygiene (HH) is generally accepted, studies continue to document inadequate compliance. Medical students are taught about the importance of HH to prevent nosocomial infections, and receive training in the correct procedures for HH. However, personality traits (social orientation and achievement orientation) may influence HH compliance. People with high social orientation feel socially responsible and act cooperatively, and people with high achievement orientation are ambitious and competitive.
    Aim: To evaluate the relationship between HH compliance and personality traits of medical students.
    Methods: The HH compliance of 155 students was observed during objective standardized clinical examinations (OSCEs). Social orientation and achievement orientation were measured using the corresponding scales of the Freiburg Personality Inventory - Revised.
    Findings: Social orientation did not differ between students with high HH compliance and students with low HH compliance [F(1) = 3.87, P = 0.052, η(2) = 0.045]. For achievement orientation, a moderate effect was found between low and high HH compliance [F(1) = 11.242, P = 0.001, η(2) = 0.119], and students with high HH compliance were found to be more achievement orientated than students with low HH compliance.
    Conclusion: Achievement orientation plays a major role during OSCEs, while social orientation is less emphasized. To the authors' knowledge, this is the first study to show that HH compliance is associated with achievement orientation in achievement situations.
    MeSH term(s) Cohort Studies ; Compliance ; Cross Infection/prevention & control ; Female ; Germany ; Guideline Adherence ; Hand Disinfection ; Health Knowledge, Attitudes, Practice ; Humans ; Male ; Personality ; Physical Examination/methods ; Physical Examination/standards ; Students, Medical/psychology
    Language English
    Publishing date 2015-03
    Publishing country England
    Document type Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
    ZDB-ID 779366-2
    ISSN 1532-2939 ; 0195-6701
    ISSN (online) 1532-2939
    ISSN 0195-6701
    DOI 10.1016/j.jhin.2014.11.014
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  10. Article ; Online: A Glycolipidated-liposomal peptide vaccine confers long-term mucosal protection against Streptococcus pyogenes via IL-17, macrophages and neutrophils.

    Ozberk, Victoria / Zaman, Mehfuz / Lepletier, Ailin / Eskandari, Sharareh / Kaden, Jacqualine / Mills, Jamie-Lee / Calcutt, Ainslie / Dooley, Jessica / Huo, Yongbao / Langshaw, Emma L / Ulett, Glen C / Batzloff, Michael R / Good, Michael F / Pandey, Manisha

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 5963

    Abstract: Mucosally active subunit vaccines are an unmet clinical need due to lack of licensed immunostimulants suitable for vaccine antigens. Here, we show that intranasal administration of liposomes incorporating: the Streptococcus pyogenes peptide antigen, J8; ... ...

    Abstract Mucosally active subunit vaccines are an unmet clinical need due to lack of licensed immunostimulants suitable for vaccine antigens. Here, we show that intranasal administration of liposomes incorporating: the Streptococcus pyogenes peptide antigen, J8; diphtheria toxoid as a source of T cell help; and the immunostimulatory glycolipid, 3D(6-acyl) PHAD (PHAD), is able to induce long-lived humoral and cellular immunity. Mice genetically deficient in either mucosal antibodies or total antibodies are protected against S. pyogenes respiratory tract infection. Utilizing IL-17-deficient mice or depleting cellular subsets using antibodies, shows that the cellular responses encompassing, CD4
    MeSH term(s) Mice ; Animals ; Liposomes ; Streptococcus pyogenes ; Neutrophils ; Interleukin-17 ; Antigens, Bacterial ; Macrophages ; Administration, Intranasal ; Immunity, Mucosal ; Vaccines, Subunit ; Mice, Inbred BALB C
    Chemical Substances Liposomes ; Interleukin-17 ; Antigens, Bacterial ; Vaccines, Subunit
    Language English
    Publishing date 2023-09-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-41410-7
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