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  1. Article ; Online: Dental management before radiotherapy of the head and neck region: 4-year single-center experience.

    Hoffmann, Lea / Marschner, Sebastian N / Kakoschke, Tamara K / Hickel, Reinhard / Sabbagh, Hisham / Wölfle, Uta C

    Clinical and experimental dental research

    2022  Volume 8, Issue 6, Page(s) 1478–1486

    Abstract: Objective: To review our experience with a standardized dental management approach in patients with planned radiotherapy of the head and neck region based on preradiation and follow-up data.: Material and methods: Records of patients who underwent ... ...

    Abstract Objective: To review our experience with a standardized dental management approach in patients with planned radiotherapy of the head and neck region based on preradiation and follow-up data.
    Material and methods: Records of patients who underwent radiotherapy between June 2016 and November 2020 were reviewed. Data on dental findings and therapeutic recommendations were extracted from a prospectively managed database. Hospital records were used to obtain follow-up data.
    Results: Two hundred eighty-one patient records were identified. After the exclusion of 81 patients because of incomplete data, 200 patients were included in the study. Dental findings relevant to radiotherapy were found in 144 cases (72.0%). Teeth extractions were recommended in 112 (56.0%) patients. Follow-up data were available for 172 (86.0%) patients (mean follow-up: 16.8 ± 10.7 months). Radiodermatitis was the most frequently observed sequela of radiotherapy (42.4%), followed by dysphagia (38.4%) and stomatitis (36.6%). Osteoradionecrosis was observed in only 2.3% of the patients.
    Conclusion: Dental findings relevant to planned radiotherapy were frequent and in many cases resulted in recommendations for teeth extraction. Based on our standardized dental management protocol, we observed low rates of late oral complications after radiotherapy of the head and neck region.
    MeSH term(s) Humans ; Head and Neck Neoplasms/radiotherapy ; Head and Neck Neoplasms/complications ; Osteoradionecrosis/epidemiology ; Osteoradionecrosis/etiology ; Tooth Extraction/adverse effects ; Neck ; Dental Care
    Language English
    Publishing date 2022-09-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2829558-4
    ISSN 2057-4347 ; 2057-4347
    ISSN (online) 2057-4347
    ISSN 2057-4347
    DOI 10.1002/cre2.662
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Tape mount immunostaining: a versatile method for immunofluorescence analysis of fungi.

    Kakoschke, Tamara K / Kleinemeier, Christoph / Langenmayer, Martin C / Ebel, Frank

    Future microbiology

    2019  Volume 14, Page(s) 275–282

    Abstract: Aim: Immunofluorescence microscopy is a powerful technique to detect surface antigens and study their distribution. Analysis of fungi is often hampered by their weak adherence to glass. We therefore established a novel immunofluorescence staining method ...

    Abstract Aim: Immunofluorescence microscopy is a powerful technique to detect surface antigens and study their distribution. Analysis of fungi is often hampered by their weak adherence to glass. We therefore established a novel immunofluorescence staining method to overcome this problem.
    Materials & methods: Fungal material from colonies is bound to adhesive tape and stained with antibodies.
    Results: The obtained samples had very good optical quality, showing low unspecific background staining and allowing analysis by confocal laser scanning microscopy. We have exemplified applying the new method to study the distribution of galactomannan on conidiophores of Aspergillus fumigatus and of β-glucans on Malassezia pachydermatis.
    Conclusion: Tape mount immunostaining facilitates analysis of fungal surface molecules and provides a base for expeditious diagnostic procedures.
    MeSH term(s) Adhesives/chemistry ; Aspergillus fumigatus/chemistry ; Aspergillus fumigatus/metabolism ; Fluorescent Antibody Technique/instrumentation ; Fluorescent Antibody Technique/methods ; Humans ; Malassezia/chemistry ; Malassezia/metabolism ; Mannans/metabolism ; Staining and Labeling/instrumentation ; Staining and Labeling/methods ; beta-Glucans/metabolism
    Chemical Substances Adhesives ; Mannans ; beta-Glucans ; galactomannan (11078-30-1)
    Language English
    Publishing date 2019-02-13
    Publishing country England
    Document type Evaluation Studies ; Journal Article
    ISSN 1746-0921
    ISSN (online) 1746-0921
    DOI 10.2217/fmb-2018-0283
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Accuracy of maxillary positioning using computer-designed and manufactured occlusal splints or patient-specific implants in orthognathic surgery.

    Malenova, Yoana / Ortner, Florian / Liokatis, Paris / Haidari, Selgai / Tröltzsch, Matthias / Fegg, Florian / Obermeier, Katharina T / Hartung, Jens T / Kakoschke, Tamara K / Burian, Egon / Otto, Sven / Sabbagh, Hisham / Probst, Florian A

    Clinical oral investigations

    2023  Volume 27, Issue 9, Page(s) 5063–5072

    Abstract: Objective: To determine the accuracy of maxillary positioning using computer-designed and manufactured occlusal splints or patient-specific implants in orthognathic surgery.: Material and methods: A retrospective analysis of 28 patients that ... ...

    Abstract Objective: To determine the accuracy of maxillary positioning using computer-designed and manufactured occlusal splints or patient-specific implants in orthognathic surgery.
    Material and methods: A retrospective analysis of 28 patients that underwent virtually planned orthognathic surgery with maxillary Le Fort I osteotomy either using VSP-generated splints (n = 13) or patient-specific implants (PSI) (n = 15) was conducted. The accuracy and surgical outcome of both techniques were compared by superimposing preoperative surgical planning with postoperative CT scans and measurement of translational and rotational deviation for each patient.
    Results: The 3D global geometric deviation between the planned position and the postoperative outcome was 0.60 mm (95%-CI 0.46-0.74, range 0.32-1.11 mm) for patients with PSI and 0.86 mm (95%-CI 0.44-1.28, range 0.09-2.60 mm) for patients with surgical splints. Postoperative differences for absolute and signed single linear deviations between planned and postoperative position were a little higher regarding the x-axis and pitch but lower regarding the y- and z-axis as well as yaw and roll for PSI compared to surgical splints. There were no significant differences regarding global geometric deviation, absolute and signed linear deviations in the x-, y-, and z-axis, and rotations (yaw, pitch, and roll) between both groups.
    Conclusions: Regarding accuracy for positioning of maxillary segments after Le Fort I osteotomy in orthognathic surgery patient-specific implants and surgical splints provide equivalent high accuracy.
    Clinical relevance: Patient-specific implants for maxillary positioning and fixation facilitate the concept of splintless orthognathic surgery and can be reliably used in clinical routines.
    MeSH term(s) Humans ; Orthognathic Surgery ; Occlusal Splints ; Orthognathic Surgical Procedures/methods ; Retrospective Studies ; Surgery, Computer-Assisted/methods ; Dental Implants ; Maxilla/surgery ; Computers ; Imaging, Three-Dimensional/methods ; Osteotomy, Le Fort/methods
    Chemical Substances Dental Implants
    Language English
    Publishing date 2023-06-29
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1364490-7
    ISSN 1436-3771 ; 1432-6981
    ISSN (online) 1436-3771
    ISSN 1432-6981
    DOI 10.1007/s00784-023-05125-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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