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  1. Article ; Online: Expression of estrogen-, progesterone-, and androgen-responsive genes in MCF-7 and MDA-MB-231 cells treated with o,p'-DDT, p,p'-DDT, or endosulfan.

    Kalinina, Tatiana S / Kononchuk, Vladislav V / Gulyaeva, Lyudmila F

    Journal of biochemical and molecular toxicology

    2021  Volume 35, Issue 6, Page(s) 1–8

    Abstract: Endocrine disruptors are a major concern due to their possible association with hormone-dependent carcinogenesis. Some examples of compounds with such properties are organochlorine pesticides (OCPs). OCPs are persistent pollutants with high lipophilicity, ...

    Abstract Endocrine disruptors are a major concern due to their possible association with hormone-dependent carcinogenesis. Some examples of compounds with such properties are organochlorine pesticides (OCPs). OCPs are persistent pollutants with high lipophilicity, long half-life, and bioaccumulation potential. In the past, some of the most commonly used OCPs were dichlorodiphenyltrichloroethane (DDT) and endosulfan. Here, we investigated the effects of o,p'-DDT, p,p'-DDT, and endosulfan and of hormones estradiol, testosterone, and progesterone on the expression of estrogen, progesterone, and androgen receptors (ER, PR, and AR) and of their target genes (KLF4, VEGFA, CCND1, PRLR, CDKN1A, and BCL6) in MCF-7 and MDA-MB-231 cells. The results confirmed that under the action of the insecticides, there are dose- and time-dependent changes in the expression of these receptors and target genes. As corroborated by an experiment with ER, PR, and AR negative MDA-MB-231 cells, the change in the expression of KLF4, VEGFA, CCND1, and PRLR in MCF-7 cells treated with o,p'-DDT and the change in CDKN1A and PRLR expression in MCF-7 cells treated with p,p'-DDT are likely mediated by ER, PR, and AR pathways. In conclusion, we have identified some targets of DDT and endosulfan and confirmed that the effects of insecticides on the expression of these target genes differ for breast cancer cell lines with different receptor statuses.
    Language English
    Publishing date 2021-03-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1410020-4
    ISSN 1099-0461 ; 1095-6670
    ISSN (online) 1099-0461
    ISSN 1095-6670
    DOI 10.1002/jbt.22773
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Changes in Gene Expression and Neuroinflammation in the Hippocampus after Focal Brain Ischemia: Involvement in the Long-Term Cognitive and Mental Disorders.

    Shishkina, Galina T / Kalinina, Tatiana S / Gulyaeva, Natalia V / Lanshakov, Dmitry A / Dygalo, Nikolay N

    Biochemistry. Biokhimiia

    2021  Volume 86, Issue 6, Page(s) 657–666

    Abstract: Ischemic brain injuries are accompanied by the long-term changes in gene expression in the hippocampus, the limbic system structure, involved in the regulation of key aspects of the higher nervous activity, such as cognitive functions and emotions. The ... ...

    Abstract Ischemic brain injuries are accompanied by the long-term changes in gene expression in the hippocampus, the limbic system structure, involved in the regulation of key aspects of the higher nervous activity, such as cognitive functions and emotions. The altered expression of genes and proteins encoded by them may be related to the development of post-ischemic psycho-emotional and cognitive disturbances. Activation of neuroinflammation following stroke in the hippocampus has been suggested to play an essential role in induction of long-lasting consequences. Identification of changes in the gene expression patterns after ischemia and investigation of the dynamics of these changes in the hippocampus are the necessary first steps toward understanding molecular pathways responsible for the development of post-stroke cognitive impairments and mental pathologies.
    MeSH term(s) Animals ; Brain Injuries ; Brain Ischemia/complications ; Brain Ischemia/genetics ; Brain Ischemia/metabolism ; Cognition ; Cognition Disorders/etiology ; Depression/etiology ; Gene Expression Regulation ; Hippocampus/metabolism ; Humans ; Inflammation ; Mental Disorders/etiology ; Stroke/complications ; Stroke/genetics ; Stroke/metabolism
    Language English
    Publishing date 2021-07-06
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1109-5
    ISSN 1608-3040 ; 0006-2979 ; 0320-9717
    ISSN (online) 1608-3040
    ISSN 0006-2979 ; 0320-9717
    DOI 10.1134/S0006297921060043
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Antiviral activity of the high-molecular-weight plant polysaccharides (Panavir®).

    Kalinina, Tatiana S / Zlenko, Dmitry V / Kiselev, Aleksei V / Litvin, Alexander A / Stovbun, Sergey V

    International journal of biological macromolecules

    2020  Volume 161, Page(s) 936–938

    Abstract: This short report is dedicated to the description of the wide antiviral and antibacterial activity of the immune-modulating agent Panavir®. Panavir® is a high-molecular-weight fraction of the polysaccharides extracted from the shoots of the Solanum ... ...

    Abstract This short report is dedicated to the description of the wide antiviral and antibacterial activity of the immune-modulating agent Panavir®. Panavir® is a high-molecular-weight fraction of the polysaccharides extracted from the shoots of the Solanum tuberosum. It demonstrates activity against many types of viruses, including animal coronavirus and also against bacterial infections. These properties look very promising considering the COVID-19 epidemy and allow propose that Panavir® would be effective in the therapy of the SARS-CoV-2 infection.
    MeSH term(s) Adult ; Animals ; Antiviral Agents/chemistry ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Betacoronavirus/drug effects ; COVID-19 ; Coronavirus Infections/drug therapy ; Coronavirus Infections/virology ; Disease Models, Animal ; Dysentery, Bacillary/drug therapy ; Female ; Glycosides/chemistry ; Glycosides/pharmacology ; Glycosides/therapeutic use ; Herpes Genitalis/drug therapy ; Humans ; Klebsiella Infections/drug therapy ; Klebsiella pneumoniae/drug effects ; Male ; Mice ; Middle Aged ; Pandemics ; Pneumonia, Viral/drug therapy ; Pneumonia, Viral/virology ; SARS-CoV-2 ; Shigella flexneri/drug effects ; Young Adult
    Chemical Substances Antiviral Agents ; Glycosides ; Panavir (Solanum tuberosum)
    Keywords covid19
    Language English
    Publishing date 2020-06-11
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2020.06.031
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Antiviral potential of plant polysaccharide nanoparticles actuating non-specific immunity.

    Stovbun, Sergey V / Kalinina, Tatiana S / Zlenko, Dmitry V / Kiselev, Aleksei V / Litvin, Alexander A / Bukhvostov, Alexander A / Usachev, Sergey V / Kuznetsov, Dmitry A

    International journal of biological macromolecules

    2021  Volume 182, Page(s) 743–749

    Abstract: The development of high-end targeted drugs and vaccines against modern pandemic infections, such as COVID-19, can take a too long time that lets the epidemic spin up and harms society. However, the countermeasures must be applied against the infection in ...

    Abstract The development of high-end targeted drugs and vaccines against modern pandemic infections, such as COVID-19, can take a too long time that lets the epidemic spin up and harms society. However, the countermeasures must be applied against the infection in this period until the targeted drugs became available. In this regard, the non-specific, broad-spectrum anti-viral means could be considered as a compromise allowing overcoming the period of trial. One way to enhance the ability to resist the infection is to activate the nonspecific immunity using a suitable driving-up agent, such as plant polysaccharides, particularly our drug Panavir isolated from the potato shoots. Earlier, we have shown the noticeable anti-viral and anti-bacterial activity of Panavir. Here we demonstrate the pro-inflammation activity of Panavir, which four-to-eight times intensified the ATP and MIF secretion by HL-60 cells. This effect was mediated by the active phagocytosis of the Panavir particles by the cells. We hypothesized the physiological basis of the Panavir proinflammatory activity is mediated by the indol-containing compounds (auxins) present in Panavir and acting as a plant analog of serotonin.
    MeSH term(s) Adolescent ; Adult ; Antiviral Agents/chemistry ; Antiviral Agents/pharmacokinetics ; Antiviral Agents/pharmacology ; Drug Carriers/chemistry ; Drug Carriers/pharmacokinetics ; Drug Carriers/pharmacology ; HL-60 Cells ; Humans ; Male ; Nanoparticles/chemistry ; Nanoparticles/therapeutic use ; Phagocytosis/drug effects ; Plants/chemistry ; Polysaccharides/chemistry ; Polysaccharides/pharmacology ; Probucol/chemistry ; Probucol/pharmacokinetics ; Probucol/pharmacology ; COVID-19 Drug Treatment
    Chemical Substances Antiviral Agents ; Drug Carriers ; Polysaccharides ; Probucol (P3CTH044XJ)
    Language English
    Publishing date 2021-04-05
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2021.03.135
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Antiviral activity of the high-molecular-weight plant polysaccharides (Panavir®)

    Kalinina, Tatiana S / Zlenko, Dmitry V / Kiselev, Aleksei V / Litvin, Alexander A / Stovbun, Sergey V

    International journal of biological macromolecules. 2020 Oct. 15, v. 161

    2020  

    Abstract: This short report is dedicated to the description of the wide antiviral and antibacterial activity of the immune-modulating agent Panavir®. Panavir® is a high-molecular-weight fraction of the polysaccharides extracted from the shoots of the Solanum ... ...

    Abstract This short report is dedicated to the description of the wide antiviral and antibacterial activity of the immune-modulating agent Panavir®. Panavir® is a high-molecular-weight fraction of the polysaccharides extracted from the shoots of the Solanum tuberosum. It demonstrates activity against many types of viruses, including animal coronavirus and also against bacterial infections. These properties look very promising considering the COVID-19 epidemy and allow propose that Panavir® would be effective in the therapy of the SARS-CoV-2 infection.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; Solanum tuberosum ; animals ; antibacterial properties ; antiviral properties ; immunomodulators ; polysaccharides ; therapeutics
    Language English
    Dates of publication 2020-1015
    Size p. 936-938.
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2020.06.031
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Association between Lymph Node Status and Expression Levels of Androgen Receptor, miR-185, miR-205, and miR-21 in Breast Cancer Subtypes.

    Kalinina, Tatiana S / Kononchuk, Vladislav V / Yakovleva, Alisa K / Alekseenok, Efim Y / Sidorov, Sergey V / Gulyaeva, Lyudmila F

    International journal of breast cancer

    2020  Volume 2020, Page(s) 3259393

    Abstract: Breast cancer is the most commonly diagnosed cancer among women. Difficulties in treating breast cancer are associated with the occurrence of metastases at early stages of disease, leading to its further progression. Recent studies have shown that ... ...

    Abstract Breast cancer is the most commonly diagnosed cancer among women. Difficulties in treating breast cancer are associated with the occurrence of metastases at early stages of disease, leading to its further progression. Recent studies have shown that changes in androgen receptor (AR) and microRNAs' expressions are associated with mammary gland carcinogenesis, in particular, with the formation of metastases. Thus, to identify novel metastatic markers, we evaluated the expression levels of AR; miR-185 and miR-205, both of which have been confirmed to target AR; and miR-21, transcription of which is regulated by AR, in breast cancer samples (
    Language English
    Publishing date 2020-04-23
    Publishing country Egypt
    Document type Journal Article
    ZDB-ID 2603566-2
    ISSN 2090-3189 ; 2090-3170
    ISSN (online) 2090-3189
    ISSN 2090-3170
    DOI 10.1155/2020/3259393
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro.

    Kalinina, Tatiana S / Kononchuk, Vladislav V / Ovchinnikov, Vladimir Y / Chanyshev, Mikhail D / Gulyaeva, Lyudmila F

    Molecular biology reports

    2018  Volume 45, Issue 6, Page(s) 1937–1945

    Abstract: A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially ... ...

    Abstract A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially regulated by CAR and ER receptors activated by DDT. We showed that exposure to DDT results in a dose- and organ-dependent increase in the expression of miR-190a, -190b in the liver, uterus, ovaries and mammary gland of female Wistar rats. Additionally, we demonstrate a decrease in protein product level of Tp53inp1, the target gene of these microRNAs, in the rat uterus. It is known that miR-190 is probably regulated by ER in humans, thus we measured the level of miR-190a, -190b in primary cultures of malignant and normal human endometrial cells treated with different doses of DDT. We detected an increase in miR-190b level in normal endometrial cells under DDT exposure. Thus, our results indicate that DDT exposure lead to change in the expression of oncogenic miR-190 family and its target gene Tp53inp1 which may be due to activation of CAR and ER.
    MeSH term(s) Animals ; Computer Simulation ; DDT/adverse effects ; Female ; Humans ; Insecticides/adverse effects ; Liver/drug effects ; Mammary Glands, Human/drug effects ; MicroRNAs/drug effects ; MicroRNAs/genetics ; Ovary/drug effects ; RNA, Messenger ; Rats ; Rats, Wistar ; Receptors, Cytoplasmic and Nuclear/drug effects ; Receptors, Cytoplasmic and Nuclear/genetics ; Receptors, Estrogen/drug effects ; Receptors, Estrogen/genetics ; Uterus/drug effects
    Chemical Substances Insecticides ; MIRN190 microRNA, rat ; MicroRNAs ; RNA, Messenger ; Receptors, Cytoplasmic and Nuclear ; Receptors, Estrogen ; constitutive androstane receptor (438XLITDI3) ; DDT (CIW5S16655)
    Language English
    Publishing date 2018-11-12
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 186544-4
    ISSN 1573-4978 ; 0301-4851
    ISSN (online) 1573-4978
    ISSN 0301-4851
    DOI 10.1007/s11033-018-4343-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Antiviral activity of the high-molecular-weight plant polysaccharides (Panavir®)

    Kalinina, Tatiana S / Zlenko, Dmitry V / Kiselev, Aleksei V / Litvin, Alexander A / Stovbun, Sergey V

    Int J Biol Macromol

    Abstract: This short report is dedicated to the description of the wide antiviral and antibacterial activity of the immune-modulating agent Panavir®. Panavir® is a high-molecular-weight fraction of the polysaccharides extracted from the shoots of the Solanum ... ...

    Abstract This short report is dedicated to the description of the wide antiviral and antibacterial activity of the immune-modulating agent Panavir®. Panavir® is a high-molecular-weight fraction of the polysaccharides extracted from the shoots of the Solanum tuberosum. It demonstrates activity against many types of viruses, including animal coronavirus and also against bacterial infections. These properties look very promising considering the COVID-19 epidemy and allow propose that Panavir® would be effective in the therapy of the SARS-CoV-2 infection.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #593423
    Database COVID19

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  9. Article: Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro

    Kalinina, Tatiana S / Chanyshev, Mikhail D / Gulyaeva, Lyudmila F / Kononchuk, Vladislav V / Ovchinnikov, Vladimir Y

    Molecular biology reports. 2018 Dec., v. 45, no. 6

    2018  

    Abstract: A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially ... ...

    Abstract A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially regulated by CAR and ER receptors activated by DDT. We showed that exposure to DDT results in a dose- and organ-dependent increase in the expression of miR-190a, -190b in the liver, uterus, ovaries and mammary gland of female Wistar rats. Additionally, we demonstrate a decrease in protein product level of Tp53inp1, the target gene of these microRNAs, in the rat uterus. It is known that miR-190 is probably regulated by ER in humans, thus we measured the level of miR-190a, -190b in primary cultures of malignant and normal human endometrial cells treated with different doses of DDT. We detected an increase in miR-190b level in normal endometrial cells under DDT exposure. Thus, our results indicate that DDT exposure lead to change in the expression of oncogenic miR-190 family and its target gene Tp53inp1 which may be due to activation of CAR and ER.
    Keywords animal ovaries ; DDT (pesticide) ; endometrium ; females ; humans ; insecticides ; liver ; mammary glands ; mechanism of action ; messenger RNA ; microRNA ; rats ; receptors
    Language English
    Dates of publication 2018-12
    Size p. 1937-1945.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 186544-4
    ISSN 1573-4978 ; 0301-4851
    ISSN (online) 1573-4978
    ISSN 0301-4851
    DOI 10.1007/s11033-018-4343-0
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Analysis of Antidepressant-like Effects and Action Mechanisms of GSB-106, a Small Molecule, Affecting the TrkB Signaling.

    Vakhitova, Yulia V / Kalinina, Tatiana S / Zainullina, Liana F / Lusta, Anastasiya Yu / Volkova, Anna V / Kudryashov, Nikita V / Gudasheva, Tatiana A / Shimshirt, Alexander A / Kadnikov, Ilya A / Voronin, Mikhail V / Seredenin, Sergei B

    International journal of molecular sciences

    2021  Volume 22, Issue 24

    Abstract: Induction of BDNF-TrkB signaling is associated with the action mechanisms of conventional and fast-acting antidepressants. GSB-106, developed as a small dimeric dipeptide mimetic of BDNF, was previously shown to produce antidepressant-like effects in the ...

    Abstract Induction of BDNF-TrkB signaling is associated with the action mechanisms of conventional and fast-acting antidepressants. GSB-106, developed as a small dimeric dipeptide mimetic of BDNF, was previously shown to produce antidepressant-like effects in the mouse Porsolt test, tail suspension test, Nomura water wheel test, in the chronic social defeat stress model and in the inflammation-induced model of depression. In the present study, we evaluated the effect of chronic per os administration of GSB-106 to Balb/c mice under unpredictable chronic mild stress (UCMS). It was observed for the first time that long term GSB-106 treatment (1 mg/kg, 26 days) during ongoing UCMS procedure ameliorated the depressive-like behaviors in mice as indicated by the Porsolt test. In addition, chronic per os administration of GSB-106 resulted in an increase in BDNF levels, which were found to be decreased in the prefrontal cortex and hippocampus of mice after UCMS. Furthermore, prolonged GSB-106 treatment was accompanied by an increase in the content of pTrkB
    MeSH term(s) Animals ; Antidepressive Agents/pharmacology ; Brain/metabolism ; Brain-Derived Neurotrophic Factor/drug effects ; Brain-Derived Neurotrophic Factor/metabolism ; Dipeptides/metabolism ; Dipeptides/pharmacology ; Hippocampus/drug effects ; Hippocampus/metabolism ; Male ; Membrane Glycoproteins/drug effects ; Membrane Glycoproteins/metabolism ; Mice ; Mice, Inbred BALB C ; Prefrontal Cortex/drug effects ; Prefrontal Cortex/metabolism ; Protein-Tyrosine Kinases/drug effects ; Protein-Tyrosine Kinases/metabolism ; Signal Transduction/drug effects ; Stress, Psychological
    Chemical Substances Antidepressive Agents ; Brain-Derived Neurotrophic Factor ; Dipeptides ; Membrane Glycoproteins ; bis-(N-monosuccinyl-l-seryl-l-lysine)hexamethylenediamide ; Ntrk2 protein, mouse (EC 2.7.10.1) ; Protein-Tyrosine Kinases (EC 2.7.10.1)
    Language English
    Publishing date 2021-12-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms222413381
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