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  1. Article: Bacterial safety study of the production process of hemoglobin-based oxygen carriers.

    Steffen, Axel / Xiong, Yu / Georgieva, Radostina / Kalus, Ulrich / Bäumler, Hans

    Beilstein journal of nanotechnology

    2022  Volume 13, Page(s) 114–126

    Abstract: Hemoglobin microparticles (HbMP) produced with a three-step procedure, including coprecipitation of hemoglobin with manganese carbonate, protein cross-linking, and dissolution of the carbonate template were shown to be suitable for application as ... ...

    Abstract Hemoglobin microparticles (HbMP) produced with a three-step procedure, including coprecipitation of hemoglobin with manganese carbonate, protein cross-linking, and dissolution of the carbonate template were shown to be suitable for application as artificial oxygen carriers. First preclinical safety investigations delivered promising results. Bacterial safety plays a decisive role during the production of HbMP. Therefore, the bioburden and endotoxin content of the starting materials (especially hemoglobin) and the final particle suspension are intensively tested. However, some bacteria may not be detected by standard tests due to low concentration. The aim of this study was to investigate how these bacteria would behave in the fabrication process. Biocidal effects are known for glutaraldehyde and for ethylenediaminetetraacetic acid, chemicals that are used in the fabrication process of HbMP. It was shown that both chemicals prevent bacterial growth at the concentrations used during HbMP fabrication. In addition, the particle production was carried out with hemoglobin solutions spiked with
    Language English
    Publishing date 2022-01-24
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2583584-1
    ISSN 2190-4286
    ISSN 2190-4286
    DOI 10.3762/bjnano.13.8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book ; Thesis: Thema:Wirksamkeit von kombiniertem Ginkgo biloba und Panax ginseng auf Blutfluidität und retinale Mikrozirkulation bei Patienten mit cerebraler Mikroangiopathie

    Kalus, Ulrich Johannes

    1994  

    Author's details vorgelegt von Ulrich Johannes Kalus
    Language German
    Size 59 Bl. : graph. Darst.
    Edition [Mikrofiche-Ausg.]
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Berlin, Humboldt-Univ., Diss., 1995
    Note Mikrofiche-Ausg.: 2 Mikrofiches : 24x
    HBZ-ID HT006780479
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    1995 MB 2268 order for loan Magazin

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  3. Article: Recognition mechanisms of hemoglobin particles by monocytes - CD163 may just be one.

    Nimz, Jonathan-Gabriel / Rerkshanandana, Pichayut / Kloypan, Chiraphat / Kalus, Ulrich / Chaiwaree, Saranya / Pruß, Axel / Georgieva, Radostina / Xiong, Yu / Bäumler, Hans

    Beilstein journal of nanotechnology

    2023  Volume 14, Page(s) 1028–1040

    Abstract: Hemoglobin-based oxygen carriers (HBOCs) as blood substitutes are one of the great hopes of modern transfusion and emergency medicine. After the major safety-relevant challenges of the last decades seem to be largely overcome, current developments have ... ...

    Abstract Hemoglobin-based oxygen carriers (HBOCs) as blood substitutes are one of the great hopes of modern transfusion and emergency medicine. After the major safety-relevant challenges of the last decades seem to be largely overcome, current developments have in common that they are affected by degradation and excretion at an early stage in test organisms. Several possible mechanisms that may be responsible for this are discussed in the literature. One of them is CD163, the receptor of the complex of haptoglobin (Hp) and hemoglobin (Hb). The receptor has been shown in various studies to have a direct affinity for Hb in the absence of Hp. Thus, it seems reasonable that CD163 could possibly also bind Hb within HBOCs and cause phagocytosis of the particles. In this work we investigated the role of CD163 in the uptake of our hemoglobin sub-micron particles (HbMPs) in monocytes and additionally screened for alternative ways of particle recognition by monocytes. In our experiments, blockade of CD163 by specific monoclonal antibodies proved to partly inhibit HbMP uptake by monocytes. It appears, however, that several other phagocytosis pathways for HbMPs might exist, independent of CD163 and also Hb.
    Language English
    Publishing date 2023-10-19
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2583584-1
    ISSN 2190-4286
    ISSN 2190-4286
    DOI 10.3762/bjnano.14.85
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Adding a piece to the puzzle of Latin American blood donors and the potential risk of <i>Trypanosoma cruzi</i> transmission in Germany.

    Ullrich, Julian / Guertler, Lutz / Quenzel, Ernst / Weinauer, Franz / Rößler, Dieter / Kalus, Ulrich / Pruß, Axel / Albajar-Viñas, Pedro / Pritsch, Michael

    Frontiers in cellular and infection microbiology

    2022  Volume 12, Page(s) 1014134

    Abstract: Introduction: Chagas disease (CD) is caused by the ... Trypanosoma cruzi ... ( ... T. cruzi ... ) infection and has become a global health concern due to population mobility, as well as non-vectorial transmission routes. Several countries outside Latin ... ...

    Abstract Introduction: Chagas disease (CD) is caused by the Trypanosoma cruzi (T. cruzi) infection and has become a global health concern due to population mobility, as well as non-vectorial transmission routes. Several countries outside Latin America (LA) have reported transfusion-associated transmission, but equivalent studies in Germany are lacking. This study aims to collect first data on the risk of transfusion associated transmission as well as LA blood donors originating from CD endemic countries in Germany.
    Materials and methods: A total of 305 blood donors who were assumed to be at risk for T. cruzi infection were retrospectively (267) as well as prospectively (38) selected at German blood donation sites in Bavaria and Berlin, and all retrospectively as well as 27 prospectively selected were serologically screened. Prospective study subjects additionally filled out a questionnaire.
    Results: All samples tested seronegative for T. cuzi specific antibodies. Prospectively enrolled study subjects all had high socio-economic status including good education. Knowledge regarding CD was limited but willingness to donate frequently was high. Blood donation rates from donors born in LA countries seem to increase from 2015.
    Discussion: Although no transfusion associated T. cruzi infection has been documented in Germany, it has likely already happened unnoticed, or will do in the near future. Performing risk-adapted serology-based blood donor screenings in Germany could avoid transfusion-associated transmission events as well as contribute to active case detection. Moreover, larger, and ongoing studies are needed to increase the evidence base as well as end the neglect of CD in Germany.
    MeSH term(s) Humans ; Trypanosoma cruzi ; Blood Donors ; Latin America/epidemiology ; Retrospective Studies ; Prospective Studies ; Antibodies, Protozoan ; Chagas Disease ; Germany/epidemiology
    Chemical Substances Antibodies, Protozoan
    Language English
    Publishing date 2022-10-13
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2022.1014134
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Book ; Online ; Thesis: Neue Aspekte zu Validität und Wertigkeit der postmortalen Testung relevanter Infektionsparameter bei Gewebespendern

    Kalus, Ulrich Johannes

    2013  

    Title variant Validation of serological and NAT-testing for HIV, HBV, HCV and Treponema pallidum in postmortem blood
    Author's details vorgelegt von Ulrich Johannes Kalus
    Language German
    Size Online-Ressource, graph. Darst.
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Univ.-Medizin, Habil.--Berlin, 2013
    Database Former special subject collection: coastal and deep sea fishing

    Full text online

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  6. Book ; Online ; Thesis: Neue Aspekte zu Validität und Wertigkeit der postmortalen Testung relevanter Infektionsparameter bei Gewebespendern

    Kalus, Ulrich Johannes [Verfasser]

    2013  

    Author's details Ulrich Kalus
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Medizinische Fakultät Charité - Universitätsmedizin Berlin
    Publishing place Berlin
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

    Full text online

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  7. Article: Current trends in tissue banking.

    Pruss, Axel / Kalus, Ulrich

    Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

    2012  Volume 39, Issue 6, Page(s) 373–374

    Language English
    Publishing date 2012-09-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2100848-6
    ISSN 1660-3818 ; 1660-3796
    ISSN (online) 1660-3818
    ISSN 1660-3796
    DOI 10.1159/000345860
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 959: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  8. Article: Algorithms for the Testing of Tissue Donors for Human Immunodeficiency Virus, Hepatitis B Virus, and Hepatitis C Virus.

    Pruß, Axel / Chandrasekar, Akila / Sánchez-Ibáñez, Jacinto / Lucas-Samuel, Sophie / Kalus, Ulrich / Rabenau, Holger F

    Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

    2020  Volume 48, Issue 1, Page(s) 12–22

    Abstract: Background: Although transmission of pathogenic viruses through human tissue grafts is rare, it is still one of the most serious dreaded risks of transplantation. Therefore, in addition to the detailed medical and social history, a comprehensive ... ...

    Abstract Background: Although transmission of pathogenic viruses through human tissue grafts is rare, it is still one of the most serious dreaded risks of transplantation. Therefore, in addition to the detailed medical and social history, a comprehensive serologic and molecular screening of the tissue donors for relevant viral markers for human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) is necessary. In the case of reactive results in particular, clear decisions regarding follow-up testing and the criteria for tissue release must be made.
    Methods: Based on the clinical relevance of the specific virus markers, the sensitivity of the serological and molecular biological methods used and the application of inactivation methods, algorithms for tissue release are suggested.
    Results: Compliance with the preanalytical requirements and assessment of a possible hemodilution are mandatory requirements before testing the blood samples. While HIV testing follows defined algorithms, the procedures for HBV and HCV diagnostics are under discussion. Screening and decisions for HBV are often not as simple, e.g., due to cases of occult HBV infection, false-positive anti-HBc results, or early window period positive HBV NAT results. In the case of HCV diagnostics, modern therapies with direct-acting antivirals, which are often associated with successful treatment of the infection, should be included in the decision.
    Conclusion: In HBV and HCV testing, a high-sensitivity virus genome test should play a central role in diagnostics, especially in the case of equivocal serology, and it should be the basis for the decision to release the tissue. The proposed test algorithms and decisions are also based on current European recommendations and standards for safety and quality assurance in tissue and cell banking.
    Language English
    Publishing date 2020-12-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2100848-6
    ISSN 1660-3818 ; 1660-3796
    ISSN (online) 1660-3818
    ISSN 1660-3796
    DOI 10.1159/000513179
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 959: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article: Validation of Serological Testing for Anti-Treponema pallidum from Postmortem Blood on the Siemens-BEP(®)-III Automatic System.

    Kalus, Ulrich / Wilkemeyer, Ina / Pruss, Axel / Caspari, Gregor

    Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

    2013  Volume 40, Issue 6, Page(s) 403–408

    Abstract: Background: Infectious disease marker testing is obligatory for the release of human tissue for transplantation. Most CE-marked tests are not validated for postmortem blood. In a previous study we have validated the testing for anti-HIV-1/2, anti-HCV, ... ...

    Abstract Background: Infectious disease marker testing is obligatory for the release of human tissue for transplantation. Most CE-marked tests are not validated for postmortem blood. In a previous study we have validated the testing for anti-HIV-1/2, anti-HCV, HBsAg, and anti-HBc. Here, we present the validation of testing for antibodies against T. pallidum, which is the last marker obligatory for tissue release for transplantation.
    Methods: 17 samples of postmortem sera and 10 samples of both pre-und postmortem sera were obtained from cornea donors and tested for anti-T. pallidum on the Siemens-BEP-III-System. These sera were spiked with anti-T. pallidum-positive standard sera in concentrations which give low- and high-positive results at the respective dilution.
    Results: Two of the unspiked postmortem sera were false-positive most likely due to intense hemolysis (free hemoglobin > 50 mg/dl). Of the 25 negative postmortem sera, none of the spiked samples was false-negative after 0, 24 and 60 h.
    Conclusion: There is no indication that postmortem samples give false-negative or false-positive results with the test system and test kits used in cases of low hemolysis. The procedure described might serve as a model for validating other test kits on postmortem samples.
    Language English
    Publishing date 2013-10-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2100848-6
    ISSN 1660-3818 ; 1660-3796
    ISSN (online) 1660-3818
    ISSN 1660-3796
    DOI 10.1159/000354070
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 959: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article: Current Trends in Tissue Banking

    Pruss, Axel / Kalus, Ulrich

    Transfusion Medicine and Hemotherapy

    2012  Volume 39, Issue 6, Page(s) 373–374

    Institution University Tissue Bank, Institute of Transfusion Medicine, Charité – Universitätsmedizin Berlin, Germany
    Language English
    Publishing date 2012-11-21
    Publisher S. Karger GmbH
    Publishing place Freiburg, Germany
    Document type Article
    Note Editorial
    ZDB-ID 2100848-6
    ISSN 1660-3818 ; 1660-3796
    ISSN (online) 1660-3818
    ISSN 1660-3796
    DOI 10.1159/000345860
    Database Karger publisher's database

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 959: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    ab Jg. 2022: Lesesaal (EG)

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