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Article ; Online: G17-modified hammerhead ribozymes are active in vitro and in vivo.

Kalweit, Anne / Hammann, Christian

2013 Volume 19, Issue 12, Page(s) 1595–1604

Abstract: Natural hammerhead ribozymes (HHRz) feature tertiary interactions between hairpin loops or bulges in two of three helices that surround the catalytic core of conserved nucleotides. Their conservation was originally established on minimal versions lacking ...

Abstract	Natural hammerhead ribozymes (HHRz) feature tertiary interactions between hairpin loops or bulges in two of three helices that surround the catalytic core of conserved nucleotides. Their conservation was originally established on minimal versions lacking the tertiary interactions. While those sequence requirements in general also apply to natural versions, we show here differences for the HHRz cleavage site N17. A guanosine at this position strongly impairs cleavage activity in minimal versions, whereas we observe for the G17 variants of four tertiary stabilized HHRz significant cleavage and ligation activity in vitro. Kinetic analyses of these variants revealed a reduced rate and extent of cleavage, compared with wild-type sequences, while variants with distorted tertiary interactions cleaved at a reduced rate, but to the same extent. Contrary to this, G17 variants exhibit similar in vitro ligation activity as compared with the respective wild-type motif. To also address the catalytic performance of these motifs in vivo, we have inserted HHRz cassettes in the lacZ gene and tested this β-galactosidase reporter in Dictyostelium discoideum. In colorimetric assays, we observe differences in the enzymatic activity of β-galactosidase, which correlate well with the activity of the different HHRz variants in vitro and which can be unambiguously attributed to ribozyme cleavage by primer extension analysis.
MeSH term(s)	Animals ; Base Sequence ; Dictyostelium ; Guanosine/chemistry ; Inverted Repeat Sequences ; Kinetics ; Nucleic Acid Conformation ; RNA Cleavage ; RNA, Catalytic/chemistry ; RNA, Catalytic/genetics ; Transcription, Genetic ; Xenopus/genetics
Chemical Substances	RNA, Catalytic ; hammerhead ribozyme ; Guanosine (12133JR80S)
Language	English
Publishing date	2013-10-21
Publishing country	United States
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	1241540-6
ISSN	1469-9001 ; 1355-8382
ISSN (online)	1469-9001
ISSN	1355-8382
DOI	10.1261/rna.040543.113
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Article ; Online: One-Hour Pilot Training to Prevent Workers From Taking Home Workplace Contaminants.

Ceballos, Diana / Guerrero, Mariana / Kalweit, Andrew / Rabin, Richard / Spengler, John / Herrick, Robert

New solutions : a journal of environmental and occupational health policy : NS

2019 Volume 29, Issue 4, Page(s) 519–529

MeSH term(s)	Environmental Monitoring/standards ; Environmental Pollutants/administration & dosage ; Humans ; Occupational Exposure/prevention & control ; Occupational Health/education ; Pilot Projects ; Safety Management/organization & administration ; Workplace/organization & administration
Chemical Substances	Environmental Pollutants
Language	English
Publishing date	2019-11-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1236515-4
ISSN	1541-3772 ; 1048-2911
ISSN (online)	1541-3772
ISSN	1048-2911
DOI	10.1177/1048291119887188
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Article ; Online: Factors affecting lead dust in construction workers' homes in the Greater Boston Area.

Ceballos, Diana M / Herrick, Robert F / Dong, Zhao / Kalweit, Andrew / Miller, Melisa / Quinn, Jenna / Spengler, John D

Environmental research

2020 Volume 195, Page(s) 110510

Abstract: Lead is a known reproductive, developmental, and neurological toxicant. Workers with a high likelihood of being exposed to lead at work may inadvertently transport lead home from work, known as "take-home exposure." This is concerning for many workers ... ...

Abstract	Lead is a known reproductive, developmental, and neurological toxicant. Workers with a high likelihood of being exposed to lead at work may inadvertently transport lead home from work, known as "take-home exposure." This is concerning for many workers for whom a workplace intervention is not feasible because their worksites and employers often change, rendering centralized strategies insufficient. This study aimed to better understand the connection between lead in the home of workers living with children and work in construction (n = 23), while other occupations were used as a comparison group (janitorial n = 5, autobody n = 2). Thirty workers living in disadvantaged communities in the Greater Boston area were recruited in 2018-2019 through collaboration with non-profits and worker unions with expertise working with low-income or immigrant workers. Construction workers that performed renovations, bridge constructions, welding, metal work, and demolitions were prioritized during recruitment. During a visit to their residences, a worker questionnaire was administered, and observations and a dust vacuumed sample of the home were collected. Factors predicting lead in home dust were explored by a bivariate analysis and a multivariable regression model. We found lead in homes' dust in the range of 20-8,310 ppm. Homes of construction workers generally had higher and more variable lead dust concentrations (mean 775, max 8,300 ppm) than autobody and janitor worker homes combined (mean 296, max 579 ppm). Five of the construction workers' home lead dust concentrations exceeded US guidelines for yard soil in children's play areas of 400 ppm, and were similar to other studies of homes near lead smelters, superfund sites, or in the Boston area in the early 1990s, pointing to disparities relating to work. Results from the multivariable regression model suggest that lead dust in homes of workers was associated with sociodemographic-, home-, and work-related factors, and pointed to overlapping vulnerabilities; however, a larger sample size is needed to verify findings. Results provide evidence that work-related factors are important to consider when assessing home exposures, and that take-home exposures for workers in lead high-risk jobs such as construction may be an important source of exposure in the home prime for public health intervention at work, home, and community levels.
MeSH term(s)	Boston ; Child ; Dust/analysis ; Housing ; Humans ; Lead ; Workplace
Chemical Substances	Dust ; Lead (2P299V784P)
Language	English
Publishing date	2020-11-24
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2020.110510
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Article ; Online: Eliminating Take-Home Exposures: Recognizing the Role of Occupational Health and Safety in Broader Community Health.

Kalweit, Andrew / Herrick, Robert F / Flynn, Michael A / Spengler, John D / Berko, J Kofi / Levy, Jonathan I / Ceballos, Diana M

Annals of work exposures and health

2020 Volume 64, Issue 3, Page(s) 236–249

Abstract: Toxic contaminants inadvertently brought from the workplace to the home, known as take-home or paraoccupational exposures, have often been framed as a problem that arises due to unsanitary worker behavior. This review article conceptualizes take-home ... ...

Abstract	Toxic contaminants inadvertently brought from the workplace to the home, known as take-home or paraoccupational exposures, have often been framed as a problem that arises due to unsanitary worker behavior. This review article conceptualizes take-home exposures as a public health hazard by (i) investigating the history of take-home contaminants and how they have been studied, (ii) arguing that an ecosocial view of the problem is essential for effective prevention, (iii) summarizing key structural vulnerabilities that lead populations to be at risk, and (iv) discussing future research and prevention effort needs. This article reframes take-home exposures as one of many chronic pathways that contributes to persistent health disparities among workers, their families, and communities. Including the role of work in community health will increase the comprehensiveness of prevention efforts for contaminants such as lead and pesticides that contribute to environmental disparities.
MeSH term(s)	Family Health ; Health Status Disparities ; Humans ; Lead/adverse effects ; Occupational Exposure/analysis ; Occupational Exposure/prevention & control ; Occupational Health ; Pesticides/adverse effects ; Public Health ; Workplace
Chemical Substances	Pesticides ; Lead (2P299V784P)
Language	English
Publishing date	2020-01-29
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ZDB-ID	2885096-8
ISSN	2398-7316 ; 2398-7308
ISSN (online)	2398-7316
ISSN	2398-7308
DOI	10.1093/annweh/wxaa006
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Article ; Online: From alpaca to zebrafish: hammerhead ribozymes wherever you look.

Seehafer, Carsten / Kalweit, Anne / Steger, Gerhard / Gräf, Stefan / Hammann, Christian

RNA (New York, N.Y.)

2010 Volume 17, Issue 1, Page(s) 21–26

Abstract: The hammerhead ribozyme was originally discovered in subviral plant pathogens and was subsequently also found in a few other genomic locations. Using a secondary structure-based descriptor, we have searched publicly accessible sequence databases for new ... ...

Abstract	The hammerhead ribozyme was originally discovered in subviral plant pathogens and was subsequently also found in a few other genomic locations. Using a secondary structure-based descriptor, we have searched publicly accessible sequence databases for new examples of type III hammerhead ribozymes. The more than 60,000 entries fulfilling the descriptor were filtered with respect to folding and stability parameters that were experimentally validated. This resulted in a set of 284 unique motifs, of which 124 represent database entries of known hammerhead ribozymes from subviral plant pathogens and A. thaliana. The remainder are 160 novel ribozyme candidates in 50 different eukaryotic genomes. With a few exceptions, the ribozymes were found either in repetitive DNA sequences or in introns of protein coding genes. Our data, which is complementary to a study by De la Peña and García-Robles in 2010, indicate that the hammerhead is the most abundant small endonucleolytic ribozyme, which, in view of no sequence conservation beyond the essential nucleotides, likely has evolved independently in different organisms.
MeSH term(s)	Animals ; Base Sequence ; Camelids, New World/genetics ; Computational Biology ; Databases, Genetic ; Molecular Sequence Data ; Nucleic Acid Conformation ; RNA, Catalytic/chemistry ; RNA, Catalytic/genetics ; Zebrafish/genetics
Chemical Substances	RNA, Catalytic ; hammerhead ribozyme
Language	English
Publishing date	2010-11-16
Publishing country	United States
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	1241540-6
ISSN	1469-9001 ; 1355-8382
ISSN (online)	1469-9001
ISSN	1355-8382
DOI	10.1261/rna.2429911
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Article ; Online: Characterization of hammerhead ribozyme reactions.

Kalweit, Anne / Przybilski, Rita / Seehafer, Carsten / de la Peña, Marcos / Hammann, Christian

Methods in molecular biology (Clifton, N.J.)

2012 Volume 848, Page(s) 5–20

Abstract: Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation ... ...

Abstract	Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes. This is followed by a description of the preparation of suitable RNA molecules for both reaction types, and their kinetic analysis.
MeSH term(s)	DNA/genetics ; DNA/metabolism ; Genome/genetics ; Kinetics ; Polymerase Chain Reaction ; RNA, Catalytic/genetics ; RNA, Catalytic/metabolism ; Transcription, Genetic
Chemical Substances	RNA, Catalytic ; hammerhead ribozyme ; DNA (9007-49-2)
Language	English
Publishing date	2012
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-61779-545-9_2
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Book ; Thesis: Die Orakelheiler Lakakhs

Schenk-Kalweit, Amelie

eine Studie über Berufung, Ausbildung und Trance

1990

Author's details	vorgelegt von Amelie Schenk-Kalweit
Language	German
Size	449 Bl
Publishing place	Hamburg
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Univ., Diss.--Hamburg, 1990
Note	Literaturverz. Bl. 417 - 434
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: Global Allergy Forum and 3rd Davos Declaration 2015: Atopic dermatitis/Eczema: challenges and opportunities toward precision medicine.

Bieber, T / Akdis, C / Lauener, R / Traidl-Hoffmann, C / Schmid-Grendelmeier, P / Schäppi, G / Allam, J-P / Apfelbacher, C / Augustin, M / Beck, L / Biedermann, T / Braun-Fahrländer, C / Chew, F T / Clavel, T / Crameri, R / Darsow, U / Deleuran, M / Dittlein, D / Duchna, H-W /

Eichenfeld, L / Eyerich, K / Frei, R / Gelmetti, C / Gieler, U / Gilles, S / Glatz, M / Grando, K / Green, J / Gutermuth, J / Guttman-Yassky, E / Hanifin, J / Hijnen, D / Hoetzenecker, W / Irvine, A / Kalweit, A / Katoh, N / Knol, E / Koren, H / Möhrenschlager, M / Münch, D / Novak, N / O'Mahony, L / Paller, A S / Rhyner, C / Roduit, C / Schiesser, K / Schröder, J / Simon, D / Simon, H-U / Sokolowska, M / Spuls, P / Stalder, J-F / Straub, D / Szalai, Z / Taieb, A / Takaoka, R / Todd, G / Todorova, A / Vestergaard, C / Werfel, T / Wollenberg, A / Ring, J

Allergy

2016 Volume 71, Issue 5, Page(s) 588–592

Language	English
Publishing date	2016
Publishing country	Denmark
Document type	News
ZDB-ID	391933-x
ISSN	1398-9995 ; 0105-4538
ISSN (online)	1398-9995
ISSN	0105-4538
DOI	10.1111/all.12857
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Uh III Zs.150: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
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Article: QCD and strongly coupled gauge theories: challenges and perspectives.

Brambilla, N / Eidelman, S / Foka, P / Gardner, S / Kronfeld, A S / Alford, M G / Alkofer, R / Butenschoen, M / Cohen, T D / Erdmenger, J / Fabbietti, L / Faber, M / Goity, J L / Ketzer, B / Lin, H W / Llanes-Estrada, F J / Meyer, H B / Pakhlov, P / Pallante, E /

Polikarpov, M I / Sazdjian, H / Schmitt, A / Snow, W M / Vairo, A / Vogt, R / Vuorinen, A / Wittig, H / Arnold, P / Christakoglou, P / Di Nezza, P / Fodor, Z / Garcia I Tormo, X / Höllwieser, R / Janik, M A / Kalweit, A / Keane, D / Kiritsis, E / Mischke, A / Mizuk, R / Odyniec, G / Papadodimas, K / Pich, A / Pittau, R / Qiu, J-W / Ricciardi, G / Salgado, C A / Schwenzer, K / Stefanis, N G / von Hippel, G M / Zakharov, V I

The European physical journal. C, Particles and fields

2014 Volume 74, Issue 10, Page(s) 2981

Abstract: We highlight the progress, current status, and open challenges of QCD-driven physics, in theory and in experiment. We discuss how the strong interaction is intimately connected to a broad sweep of physical problems, in settings ranging from astrophysics ... ...

Abstract	We highlight the progress, current status, and open challenges of QCD-driven physics, in theory and in experiment. We discuss how the strong interaction is intimately connected to a broad sweep of physical problems, in settings ranging from astrophysics and cosmology to strongly coupled, complex systems in particle and condensed-matter physics, as well as to searches for physics beyond the Standard Model. We also discuss how success in describing the strong interaction impacts other fields, and, in turn, how such subjects can impact studies of the strong interaction. In the course of the work we offer a perspective on the many research streams which flow into and out of QCD, as well as a vision for future developments.
Language	English
Publishing date	2014-10-21
Publishing country	France
Document type	Review
ZDB-ID	1459069-4
ISSN	1434-6052 ; 1434-6044
ISSN (online)	1434-6052
ISSN	1434-6044
DOI	10.1140/epjc/s10052-014-2981-5
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Book ; Online: Multifunctioneel landgebruik als adaptatiestrategie

Ellen, G.J. / Hommes, S. / Kalweit, A.M. / van Lamoen, F. / Melisie, E.J. / Maring, L. / Paalman, M. / Peerdeman, K. / van Rooij, S.A.M. / Steingrover, E.G.

Puzzelen met ondernemers en beleidsmakers

2011

Abstract: Het Brabantse landelijke gebied is een dicht bevolkt en gereguleerd landschap. De vraag naar ruimte is groot en vaak conflicterend. Klimaatadaptatie maatregelen gericht op het omgaan met de effecten van klimaatverandering kunnen een extra ruimteclaim ... ...

Abstract	Het Brabantse landelijke gebied is een dicht bevolkt en gereguleerd landschap. De vraag naar ruimte is groot en vaak conflicterend. Klimaatadaptatie maatregelen gericht op het omgaan met de effecten van klimaatverandering kunnen een extra ruimteclaim leggen op de schaarse ruimte. Het project multifunctioneel landgebruik heeft de volgende resultaten opgeleverd: 1) Een overzicht en analyse van de praktische toepassing van multifunctioneel landgebruik als klimaatadaptatie strategie voor de hoge zandgronden. 2) Inzichten in hoe met actoren te komen tot ideeën voor multifunctioneel landgebruik en hoe deze te implementeren 3) Een Businesscase waarin multifunctioneel gebruik vanuit water, natuur en energie als strategie voor klimaatadaptatie is uitgewerkt.
Keywords	climatic change ; multiple land use ; nature management ; noord-brabant ; sandy soils ; water management ; klimaatverandering ; meervoudig landgebruik ; natuurbeheer ; waterbeheer ; zandgronden
Language	Dutch
Publisher	Kennis voor Klimaat
Publishing country	nl
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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