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  1. Article ; Online: Exocytosis by vesicle crumpling maintains apical membrane homeostasis during exocrine secretion.

    Kamalesh, Kumari / Scher, Nadav / Biton, Tom / Schejter, Eyal D / Shilo, Ben-Zion / Avinoam, Ori

    Developmental cell

    2021  Volume 56, Issue 11, Page(s) 1603–1616.e6

    Abstract: Exocrine secretion commonly employs micron-scale vesicles that fuse to a limited apical surface, presenting an extreme challenge for maintaining membrane homeostasis. Using Drosophila melanogaster larval salivary glands, we show that the membranes of ... ...

    Abstract Exocrine secretion commonly employs micron-scale vesicles that fuse to a limited apical surface, presenting an extreme challenge for maintaining membrane homeostasis. Using Drosophila melanogaster larval salivary glands, we show that the membranes of fused vesicles undergo actomyosin-mediated folding and retention, which prevents them from incorporating into the apical surface. In addition, the diffusion of proteins and lipids between the fused vesicle and the apical surface is limited. Actomyosin contraction and membrane crumpling are essential for recruiting clathrin-mediated endocytosis to clear the retained vesicular membrane. Finally, we also observe membrane crumpling in secretory vesicles of the mouse exocrine pancreas. We conclude that membrane sequestration by crumpling followed by targeted endocytosis of the vesicular membrane, represents a general mechanism of exocytosis that maintains membrane homeostasis in exocrine tissues that employ large secretory vesicles.
    MeSH term(s) Actin Cytoskeleton/genetics ; Actomyosin/genetics ; Animals ; Biological Transport/genetics ; Cell Membrane/genetics ; Clathrin/genetics ; Drosophila melanogaster/genetics ; Endocytosis/genetics ; Exocrine Glands/metabolism ; Exocytosis/genetics ; Homeostasis/genetics ; Membrane Fusion/genetics ; Mice ; Salivary Glands/metabolism ; Salivary Glands/physiology ; Secretory Vesicles/genetics
    Chemical Substances Clathrin ; Actomyosin (9013-26-7)
    Language English
    Publishing date 2021-05-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2054967-2
    ISSN 1878-1551 ; 1534-5807
    ISSN (online) 1878-1551
    ISSN 1534-5807
    DOI 10.1016/j.devcel.2021.05.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Phosphatidylinositol 5-phosphate 4-kinase regulates early endosomal dynamics during clathrin-mediated endocytosis.

    Kamalesh, Kumari / Trivedi, Deepti / Toscano, Sarah / Sharma, Sanjeev / Kolay, Sourav / Raghu, Padinjat

    Journal of cell science

    2017  Volume 130, Issue 13, Page(s) 2119–2133

    Abstract: Endocytic turnover is essential for the regulation of the protein composition and function of the plasma membrane, and thus affects the plasma membrane levels of many receptors. ... ...

    Abstract Endocytic turnover is essential for the regulation of the protein composition and function of the plasma membrane, and thus affects the plasma membrane levels of many receptors. In
    MeSH term(s) Absorptiometry, Photon ; Animals ; Cell Membrane/genetics ; Clathrin/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/genetics ; Endocytosis/genetics ; Phosphotransferases (Alcohol Group Acceptor)/genetics ; Phosphotransferases (Alcohol Group Acceptor)/metabolism ; Photoreceptor Cells, Invertebrate/metabolism ; Rhodopsin/genetics ; Rhodopsin/metabolism ; rab5 GTP-Binding Proteins/genetics
    Chemical Substances Clathrin ; Drosophila Proteins ; ninaE protein, Drosophila ; Rhodopsin (9009-81-8) ; Phosphotransferases (Alcohol Group Acceptor) (EC 2.7.1.-) ; Rab5 protein, Drosophila (EC 3.6.1.47.) ; rab5 GTP-Binding Proteins (EC 3.6.5.2)
    Language English
    Publishing date 2017-05-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 2993-2
    ISSN 1477-9137 ; 0021-9533
    ISSN (online) 1477-9137
    ISSN 0021-9533
    DOI 10.1242/jcs.202259
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  3. Article: Ambient temperature related sleep changes in rats neonatally treated with capsaicin.

    Gulia, Kamalesh Kumari / Mallick, Hruda Nanda / Kumar, Velayudhan Mohan

    Physiology & behavior

    2005  Volume 85, Issue 4, Page(s) 414–418

    Abstract: Ambient temperature related sleep changes in rats neonatally treated with capsaicin. PHYSIOL BEHAV 00(0) 000-000, 2004. The study was conducted on adult male Wistar rats, neonatally treated with capsaicin to destroy the peripheral warm receptors. The ... ...

    Abstract Ambient temperature related sleep changes in rats neonatally treated with capsaicin. PHYSIOL BEHAV 00(0) 000-000, 2004. The study was conducted on adult male Wistar rats, neonatally treated with capsaicin to destroy the peripheral warm receptors. The sleep-wakefulness was recorded for 5 h at an ambient temperature (T(amb)) of 18, 24, 30 and 33 degrees C on different days. The rectal temperatures (T(r)) of the rats were studied on exposure to 6 and 37 degrees C for 2 h to assess their thermoregulatory ability. The changes in the behavioral thermoregulation were assessed by noting the thermal preference of rats when they were placed in an environmental chamber with 3 interconnected compartments maintained at 24, 27 and 30 degrees C. Slow wave (SWS) and rapid eye movement (REM) sleep were decreased at 18 degrees C and increased at 30 degrees C, in control rats. There was a decrease in REM sleep and no change in SWS when T(amb) was raised from 30 to 33 degrees C. However, in neonatally capsaicin treated rats, sleep was increased even at 33 degrees C, though there was no significant change in sleep when T(amb) was increased from 18 to 24 degrees C. Capsaicin treated rats showed thermoregulatory deficiency at 37 degrees C but the thermal preference was unaltered in these rats. The results suggest that the central warm receptors can produce alteration in sleep at different T(amb), even in absence of peripheral warm receptors. The behavioral thermoregulation was unaffected in these rats, though their ability to defend the body temperature in warm environment was affected.
    MeSH term(s) Animals ; Body Temperature/drug effects ; Capsaicin/administration & dosage ; Capsaicin/pharmacology ; Electromyography ; Male ; Rats ; Rats, Wistar ; Sleep/drug effects ; Sleep/physiology ; Temperature ; Time Factors
    Chemical Substances Capsaicin (S07O44R1ZM)
    Language English
    Publishing date 2005-07-21
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3907-x
    ISSN 1873-507X ; 0031-9384
    ISSN (online) 1873-507X
    ISSN 0031-9384
    DOI 10.1016/j.physbeh.2005.05.001
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  4. Article: Sleep-related penile erections do not occur in rats during carbachol-induced rapid eye movement sleep.

    Gulia, Kamalesh Kumari / Mallick, Hruda Nanda / Kumar, Velayudhan Mohan

    Behavioural brain research

    2004  Volume 154, Issue 2, Page(s) 585–587

    Abstract: This study was undertaken to find out whether sleep-related penile erections occur in the carbachol-induced rapid eye movement sleep model in rats. Bulbospongiosus EMG, as a measure of penile erection, was recorded along with EEG, EMG, and EOG during ... ...

    Abstract This study was undertaken to find out whether sleep-related penile erections occur in the carbachol-induced rapid eye movement sleep model in rats. Bulbospongiosus EMG, as a measure of penile erection, was recorded along with EEG, EMG, and EOG during normal sleep-wakefulness. These parameters were again recorded after injection of carbachol into the pontine tegmentum. Carbachol-induced rapid eye movement sleep was not accompanied by penile erections.
    MeSH term(s) Animals ; Carbachol/pharmacology ; Cholinergic Agonists/pharmacology ; Electroencephalography/methods ; Electromyography/methods ; Electrooculography/methods ; Male ; Pedunculopontine Tegmental Nucleus/drug effects ; Pedunculopontine Tegmental Nucleus/physiology ; Penile Erection/drug effects ; Penile Erection/physiology ; Rats ; Rats, Wistar ; Sleep Stages/drug effects ; Sleep Stages/physiology ; Sleep, REM/drug effects ; Sleep, REM/physiology ; Wakefulness/drug effects ; Wakefulness/physiology
    Chemical Substances Cholinergic Agonists ; Carbachol (8Y164V895Y)
    Language English
    Publishing date 2004-10-05
    Publishing country Netherlands
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 449927-x
    ISSN 1872-7549 ; 0166-4328
    ISSN (online) 1872-7549
    ISSN 0166-4328
    DOI 10.1016/j.bbr.2004.02.019
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    Zs.A 1599: Show issues Location:
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  5. Article: Role of the lateral septal noradrenergic system in the elaboration of male sexual behavior in rats.

    Gulia, Kamalesh Kumari / Kumar, Velayudhan Mohan / Mallick, Hruda Nanda

    Pharmacology, biochemistry, and behavior

    2002  Volume 72, Issue 4, Page(s) 817–823

    Abstract: The study was aimed at investigating the possible involvement of noradrenergic mechanisms in the lateral septum (LS) for elaboration of male sexual behavior in rats. In this study, norepinephrine (NE), yohimbine (YOH), isoproterenol (ISOP), propranolol ( ... ...

    Abstract The study was aimed at investigating the possible involvement of noradrenergic mechanisms in the lateral septum (LS) for elaboration of male sexual behavior in rats. In this study, norepinephrine (NE), yohimbine (YOH), isoproterenol (ISOP), propranolol (PROP), saline (SAL) and dimethyl sulfoxide (DMSO) were injected bilaterally in the LS in six different groups of sexually active male rats, and various components of sex behavior were recorded. The application of NE (3 microg) and alpha(2)-antagonist YOH (1 microg) produced a stimulation of most of the components of male sexual behavior, and there was increase in sexual arousal as well as performance. The microinfusion of nonspecific beta-agonist ISOP (2 microg) also produced a stimulation of copulatory behavior whereas beta-antagonist PROP (2 microg) produced an inhibition. The stimulation of male sexual behavior by YOH application at the LS could be due to an increased release of NE by its blocking effect on presynaptic alpha(2)-receptors. These results suggest that the noradrenergic system in the LS has stimulatory effect upon male sexual behavior, probably acting through beta-receptors.
    MeSH term(s) Adrenergic alpha-Agonists/administration & dosage ; Adrenergic alpha-Agonists/pharmacology ; Adrenergic alpha-Antagonists/administration & dosage ; Adrenergic alpha-Antagonists/pharmacology ; Adrenergic beta-Agonists/administration & dosage ; Adrenergic beta-Agonists/pharmacology ; Adrenergic beta-Antagonists/administration & dosage ; Adrenergic beta-Antagonists/pharmacology ; Animals ; Copulation/drug effects ; Drive ; Isoproterenol/administration & dosage ; Isoproterenol/pharmacology ; Male ; Microinjections ; Norepinephrine/agonists ; Norepinephrine/antagonists & inhibitors ; Norepinephrine/physiology ; Propranolol/administration & dosage ; Propranolol/pharmacology ; Rats ; Rats, Wistar ; Septum of Brain/physiology ; Sexual Behavior, Animal/drug effects ; Sexual Behavior, Animal/physiology ; Yohimbine/pharmacology
    Chemical Substances Adrenergic alpha-Agonists ; Adrenergic alpha-Antagonists ; Adrenergic beta-Agonists ; Adrenergic beta-Antagonists ; Yohimbine (2Y49VWD90Q) ; Propranolol (9Y8NXQ24VQ) ; Isoproterenol (L628TT009W) ; Norepinephrine (X4W3ENH1CV)
    Language English
    Publishing date 2002-07
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 191042-5
    ISSN 1873-5177 ; 0091-3057
    ISSN (online) 1873-5177
    ISSN 0091-3057
    DOI 10.1016/s0091-3057(02)00771-2
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