Article ; Online: Protein-Based Immunome Wide Association Studies (PIWAS) for the Discovery of Significant Disease-Associated Antigens.
2021 Volume 12, Page(s) 625311
Abstract: Identification of the antigens associated with antibodies is vital to understanding immune responses in the context of infection, autoimmunity, and cancer. Discovering antigens at a proteome scale could enable broader identification of antigens that are ... ...
| Abstract | Identification of the antigens associated with antibodies is vital to understanding immune responses in the context of infection, autoimmunity, and cancer. Discovering antigens at a proteome scale could enable broader identification of antigens that are responsible for generating an immune response or driving a disease state. Although targeted tests for known antigens can be straightforward, discovering antigens at a proteome scale using protein and peptide arrays is time consuming and expensive. We leverage Serum Epitope Repertoire Analysis (SERA), an assay based on a random bacterial display peptide library coupled with next generation sequencing (NGS), to power the development of Protein-based Immunome Wide Association Study (PIWAS). PIWAS uses proteome-based signals to discover candidate antibody-antigen epitopes that are significantly elevated in a subset of cases compared to controls. After demonstrating statistical power relative to the magnitude and prevalence of effect in synthetic data, we apply PIWAS to systemic lupus erythematosus (SLE, n=31) and observe known autoantigens, Smith and Ribosomal protein P, within the 22 highest scoring candidate protein antigens across the entire human proteome. We validate the magnitude and location of the SLE specific signal against the Smith family of proteins using a cohort of patients who are positive by predicate anti-Sm tests. To test the generalizability of the method in an additional autoimmune disease, we identified and validated autoantigenic signals to SSB, CENPA, and keratin proteins in a cohort of individuals with Sjogren's syndrome (n=91). Collectively, these results suggest that PIWAS provides a powerful new tool to discover disease-associated serological antigens within any known proteome. |
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| MeSH term(s) | Autoantibodies/blood ; Autoantigens/genetics ; Autoantigens/immunology ; Autoantigens/metabolism ; Autoimmunity ; Case-Control Studies ; Computer Simulation ; Databases, Protein ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunodominant Epitopes ; Lupus Erythematosus, Systemic/blood ; Lupus Erythematosus, Systemic/genetics ; Lupus Erythematosus, Systemic/immunology ; Peptide Library ; Proteome ; Proteomics ; Reproducibility of Results ; Serologic Tests ; Sjogren's Syndrome/blood ; Sjogren's Syndrome/genetics ; Sjogren's Syndrome/immunology |
| Chemical Substances | Autoantibodies ; Autoantigens ; Immunodominant Epitopes ; Peptide Library ; Proteome |
| Language | English |
| Publishing date | 2021-04-27 |
| Publishing country | Switzerland |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Validation Study |
| ZDB-ID | 2606827-8 |
| ISSN | 1664-3224 ; 1664-3224 |
| ISSN (online) | 1664-3224 |
| ISSN | 1664-3224 |
| DOI | 10.3389/fimmu.2021.625311 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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