Article ; Online: In silico guided pre-treatment of sugarcane juice with natural inhibitors of polyphenol oxidase (PPO) is a new and effective strategy for development of spray-dried formulation.
2024 Volume 89, Issue 4, Page(s) 2232–2248
Abstract: Sugarcane juice is a popular beverage and is also processed to produce sugar. The polyphenol oxidase (PPO) in sugarcane juice causes enzymatic browning and makes the process of sugar production complex and cumbersome. Storage of sugarcane juice is also ... ...
| Abstract | Sugarcane juice is a popular beverage and is also processed to produce sugar. The polyphenol oxidase (PPO) in sugarcane juice causes enzymatic browning and makes the process of sugar production complex and cumbersome. Storage of sugarcane juice is also hampered by the high sugar content and rapid microbial fermentation. The present research assessed the potential of lemon juice (LJ) and ginger extract (GE) as natural inhibitors of PPO. Enzyme kinetics and the mechanism of inhibition of LJ and GE were studied. Primary investigation was carried out using molecular docking approach to assess the inhibitory potential of LJ and GE and to determine the nature of interaction between the enzyme and inhibitors. Extracts were used as inhibitors and studies revealed that both reduced the PPO activity. Subsequently, pure bioactive inhibitors such as ascorbic acid, citric acid, and 6-shogaol present in these natural extracts were used to study the mode of inhibition of PPO. Citric acid decreased PPO activity by lowering pH, while ascorbic acid was found to be a competitive inhibitor of PPO with a K |
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| MeSH term(s) | Saccharum/chemistry ; Catechol Oxidase/chemistry ; Molecular Docking Simulation ; Ascorbic Acid ; Sugars ; Citric Acid | |||||
| Chemical Substances | Catechol Oxidase (EC 1.10.3.1) ; Ascorbic Acid (PQ6CK8PD0R) ; Sugars ; Citric Acid (2968PHW8QP) | |||||
| Language | English | |||||
| Publishing date | 2024-02-21 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article | |||||
| ZDB-ID | 241615-3 | |||||
| ISSN | 1750-3841 ; 0022-1147 | |||||
| ISSN (online) | 1750-3841 | |||||
| ISSN | 0022-1147 | |||||
| DOI | 10.1111/1750-3841.16995 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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