Article ; Online: Temperature-dependent regulation of gene expression in Japanese flounder Paralichthys olivaceus kidney after Edwardsiella tarda formalin-killed cells.
2016 Volume 59, Page(s) 298–304
Abstract: Temperature affects the activities of the immune system and the susceptibility of fish to pathogens. To investigate the modulation of temperature on immune related gene expression in formalin-killed cells (FKC) of Edwardsiella tarda-injected Japanese ... ...
Abstract | Temperature affects the activities of the immune system and the susceptibility of fish to pathogens. To investigate the modulation of temperature on immune related gene expression in formalin-killed cells (FKC) of Edwardsiella tarda-injected Japanese flounder Paralichthys olivaceus, fish reared at 15 or 22 °C were injected with FKC of E. tarda. The up-regulation of immune related genes was detected in FKC-injected fish at both temperatures by qPCR. The mRNA expression of IFNγ was highly up-regulated at 6 h post injection (hpi) in FKC-injected fish at 15 °C, whereas at 22 °C, strong up-regulation of the gene was detected at 3 hpi The mRNA expression level of IRF1 was detected from 3 hpi to day 14 post injection in fish reared at 15 °C, but the gene was up-regulated from 3 to 6 hpi in fish reared at 22 °C. Comprehensive gene expression profiling showed that immune related genes are differentially expressed between 15 and 22 °C. Genes involved in the IFNγ signaling pathway were up-regulated at 22 °C but not at 15 °C. These results demonstrate that gene(s) involved in IFNγ signaling pathway in Japanese flounder stimulated with FKC of E. tarda are regulated by temperature. |
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MeSH term(s) | Animals ; Cytokines/genetics ; Cytokines/metabolism ; Edwardsiella tarda/immunology ; Fish Proteins/genetics ; Fish Proteins/metabolism ; Flatfishes/genetics ; Flatfishes/immunology ; Formaldehyde ; Gene Expression Regulation ; Hot Temperature/adverse effects ; Immunity, Innate ; Injections, Intraperitoneal/veterinary ; Kidney/immunology ; Oligonucleotide Array Sequence Analysis/veterinary |
Chemical Substances | Cytokines ; Fish Proteins ; Formaldehyde (1HG84L3525) |
Language | English |
Publishing date | 2016-12 |
Publishing country | England |
Document type | Journal Article |
ZDB-ID | 1067738-0 |
ISSN | 1095-9947 ; 1050-4648 |
ISSN (online) | 1095-9947 |
ISSN | 1050-4648 |
DOI | 10.1016/j.fsi.2016.10.048 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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