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  1. Article: Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin

    Banerjee, Pallabi / Shuvam Mukherjee / Kaushik Bera / Kanika Ghosh / Imran Ali / Sadhana Khawas / Bimalendu Ray / Sayani Ray

    International journal of biological macromolecules. 2019 Mar. 15, v. 125

    2019  

    Abstract: Thymus vulgaris is used for various health benefits and culinary, nevertheless, report on its macromolecules is absent. Here, we report chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents. ... ...

    Abstract Thymus vulgaris is used for various health benefits and culinary, nevertheless, report on its macromolecules is absent. Here, we report chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents. In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described. Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details. Biochemical analysis demonstrated dose-dependent antioxidant activity. A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites. Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M). Consequently, water extraction affords a polysaccharide which induces pharmacological effect; this underlines the impact of thyme as natural dietetic antioxidant.
    Keywords Thymus vulgaris ; acid hydrolysis ; antioxidant activity ; antioxidants ; bovine serum albumin ; chemical composition ; dose response ; electrospray ionization mass spectrometry ; enzymatic hydrolysis ; leaves ; oligosaccharides ; phenolic acids ; polysaccharides ; solvents ; thyme ; water solubility
    Language English
    Dates of publication 2019-0315
    Size p. 580-587.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2018.11.117
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Structural highlights of an antioxidative arabinogalactan protein of Lannea grandis gum that stabilizes β-lactoglobulin

    Majee, Sujay Kumar / Bimalendu Ray / Kanika Ghosh / Kaushik Bera / Sayani Ray / Washim Raja

    Food hydrocolloids. 2016 Dec., v. 61

    2016  

    Abstract: Arabinogalactan proteins (AGPs) are heavily glycosylated proteoglycans in plants. The structural complexity of their glycan portion hampers the assignment of the arabinogalactan protein function and limits their uses. Herein, we report finer structural ... ...

    Abstract Arabinogalactan proteins (AGPs) are heavily glycosylated proteoglycans in plants. The structural complexity of their glycan portion hampers the assignment of the arabinogalactan protein function and limits their uses. Herein, we report finer structural details of the AGP of Lannea grandis gum based on libraries of oligosaccharides released by Smith degradation, enzyme digestion and partial acid hydrolysis. Application of NMR spectroscopy to the parental AGP, which contained 95% carbohydrate and 3% protein, in combination with chemical, chromatographic and ESI-MS data on oligosaccharides generated allowed direct information on glycosidic linkage type, ring size, anomeric configuration and sequence of saccharide residues. Finally, this AGP shows dose-dependent free radical scavenging activity as well as forms water soluble complex (K = 3.32 × 106/M) with β-lactoglobulin (β-lg) at pH (2–7).
    Keywords acid hydrolysis ; arabinogalactan proteins ; beta-lactoglobulin ; chromatography ; dose response ; electrospray ionization mass spectrometry ; free radical scavengers ; glycosidic linkages ; glycosylation ; hydrocolloids ; Lannea coromandelica ; nuclear magnetic resonance spectroscopy ; oligosaccharides ; pH ; proteoglycans
    Language English
    Dates of publication 2016-12
    Size p. 720-729.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 742742-6
    ISSN 1873-7137 ; 0268-005X
    ISSN (online) 1873-7137
    ISSN 0268-005X
    DOI 10.1016/j.foodhyd.2016.06.016
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4414: Show issues

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  3. Article: Isolation and structural features of an antiradical polysaccharide of Capsicum annuum that interacts with BSA

    Majee, Sujay Kumar / Bimalendu Ray / Kanika Ghosh / Sayani Ray / Valérie Micard

    International journal of biological macromolecules. 2015 Apr., v. 75

    2015  

    Abstract: Red peppers, Capsicum annuum, are used worldwide as spices, foods and medicines. Herein, we have analyzed an antiradical polysaccharide isolated from red peppers through successive acetate buffer extraction. This macromolecule was purified using graded ... ...

    Abstract Red peppers, Capsicum annuum, are used worldwide as spices, foods and medicines. Herein, we have analyzed an antiradical polysaccharide isolated from red peppers through successive acetate buffer extraction. This macromolecule was purified using graded precipitation with ethanol, α-amylase treatment, deproteination and anion-exchange chromatography. This highly-branched polysaccharide (360kDa) was esterified with phenolic acids and contained a (1,3)-linked-β-Galp chain substituted at O-6 by (1,6)-linked-β-Galp residues. The latter was substituted at O-3 by (1,5)- and (1,3,5)-linked-α-Araf residues, and non-reducing end-units of α-Araf and β-Galp. The antiradical potential of this polysaccharide was comparable to standard antioxidants. The phenolic acid residues were the functional sites. This polysaccharide could form complex with bovine serum albumin having binding constant K=5.24×106/M and change its microenvironment. Thus, aqueous extraction method provides a macromolecule that stimulates biological responses; this emphasizes the significance of red pepper as dietary antioxidant.
    Keywords acetates ; alpha-amylase ; anion exchange chromatography ; antioxidants ; bovine serum albumin ; Capsicum annuum ; esterification ; ethanol ; foods ; pepper ; peppers ; phenolic acids ; polysaccharides
    Language English
    Dates of publication 2015-04
    Size p. 144-151.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2015.01.024
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: In vivo antitussive activity of a pectic arabinogalactan isolated from Solanum virginianum L. in Guinea pigs

    Raja, Washim / Bimalendu Ray / Gabriela Nosalova / Kanika Ghosh / Slavomir Nosal / Veronika Sivova

    Journal of ethnopharmacology. 2014 Oct. 28, v. 156

    2014  

    Abstract: Solanum virginianum L. is used for the management of fever, bronchial asthma and cough for thousands of years. While the link to a particular indication has been established in human, the active principle of the formulation remains unknown. Herein, we ... ...

    Abstract Solanum virginianum L. is used for the management of fever, bronchial asthma and cough for thousands of years. While the link to a particular indication has been established in human, the active principle of the formulation remains unknown. Herein, we have investigated a polysaccharide isolated from its leaves.Utilizing traditional aqueous extraction protocol and using chemical, chromatographic, spectroscopic and biological methods we have analysed an antitussive pectic arabinogalactan isolated from its leaves.The water extracted polymer (WEP) is a highly branched arabinogalactan containing, inter alia, (1,3)-, (1,6)- and (1,3,6)-linked β-Galp residues, terminal-, (1,5)- and (1,3,5)-linked units of α-Araf together with (1,2)- and (1,2,4)-linked Rhap. In vivo investigation on the citric-acid induced cough efforts in guinea pigs shows that the antitussive activity of the orally administered pectic arabinogalactan is greater than codeine phosphate. Remarkably, this macromolecule neither altered specific airway smooth muscle reactivity significantly nor it induced considerable change on levels of NO in expiratory flow in guinea pigs.Thus, traditional aqueous extraction method provides a molecular entity, which induces antitussive activity without addiction: this could represent an attractive approach in phytotherapeutic management.
    Keywords arabinogalactans ; asthma ; chromatography ; citric acid ; codeine ; cough ; fever ; guinea pigs ; nitric oxide ; oral administration ; phosphates ; phytotherapy ; polymers ; protocols ; smooth muscle ; Solanum virginianum ; spectroscopy ; traditional medicine
    Language English
    Dates of publication 2014-1028
    Size p. 41-46.
    Publishing place Elsevier Ireland Ltd
    Document type Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2014.08.012
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 90/710: Show issues

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  5. Article: In vivo cough suppressive activity of pectic polysaccharide with arabinogalactan type II side chains of Piper nigrum fruits and its synergistic effect with piperine

    Khawas, Sadhana / Bimalendu Ray / Gabriela Nosáľová / Kanika Ghosh / Sujay Kumar Majee / Veronika Sivová / Washim Raja

    International journal of biological macromolecules. 2017 June, v. 99

    2017  

    Abstract: Piper nigrum L. fruits are not only a prized spice, but also highly valued therapeutic agent that heals many ailments including asthma, cold and respiratory problems. Herein, we have investigated structural features and in vivo antitussive activity of ... ...

    Abstract Piper nigrum L. fruits are not only a prized spice, but also highly valued therapeutic agent that heals many ailments including asthma, cold and respiratory problems. Herein, we have investigated structural features and in vivo antitussive activity of three fractions isolated from Piper nigrum fruits. The water extract (PN-WE) upon fractionation with EtOH yielded two fractions: a soluble fraction (PN-eSf) and a precipitated (PN-ePf) one. The existence of a pectic polysaccharide with arabinogalactan type II side chains (147kDa) in PN-ePf and piperine in PN-eSf were revealed. Moreover, oligosaccharides providing fine structural details of side chains were generated from PN-ePf and then characterized. The parental water extract (PN-WE) that contained both pectic polysaccharide and piperine, after oral administration (50mgkg−1 body weight) to guinea pigs, showed antitussive activity comparable to codeine phosphate (10mgkg−1 body weight). The EtOH precipitated fraction (PN-ePf) containing pectic polysaccharide showed comparatively higher antitussive activity than EtOH soluble fraction (PN-eSf) that contained piperine, but their potencies are lower than the parental water extract. Significantly, the specific airway smooth muscle reactivity of all three fractions remained unchanged. Finally, pectic polysaccharide-piperine combination in parental extract synergistically enhances antitussive effect in guinea pigs.
    Keywords arabinogalactans ; asthma ; body weight ; codeine ; cough ; ethanol ; fractionation ; fruits ; guinea pigs ; oligosaccharides ; oral administration ; phosphates ; Piper nigrum ; smooth muscle ; spices ; synergism
    Language English
    Dates of publication 2017-06
    Size p. 335-342.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2017.02.093
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Interaction with bovine serum albumin of an anti-oxidative pectic arabinogalactan from Andrographis paniculata

    Chatterjee, Udipta R / Bimalendu Ray / Debjani Ghosh / Kanika Ghosh / Sayani Ray / Shruti S. Bandyopadhyay / Valérie Micard

    Carbohydrate polymers. 2014 Jan. 30, v. 101

    2014  

    Abstract: A pectic arabinogalactan was obtained from the leaves of Andrographis paniculata by aqueous extraction followed by α-amylase treatment, deproteination, and anion exchange chromatography. Methylation analysis, Smith degradation, and NMR spectroscopy ... ...

    Abstract A pectic arabinogalactan was obtained from the leaves of Andrographis paniculata by aqueous extraction followed by α-amylase treatment, deproteination, and anion exchange chromatography. Methylation analysis, Smith degradation, and NMR spectroscopy indicated that it was a highly branched arabinogalactan containing a (1→3)-linked β-d-Galp main chain, substituted at O-6 by (1→6)-linked β-d-Galp side chains. The latter residues were substituted at O-3 by (1→5)- and (1→3)-linked α-l-Araf chains, and non reducing end-units of α-l-Araf and β-d-Galp. This homogeneous arabinogalactan (36kDa), which contained phenolic acids, showed dose-dependent anti-oxidative properties. The phenolic acid moieties might be the functional sites. This arabinogalactan can form a complex with bovine serum albumin having binding constant K=6.48×106/M. Thus, this study is an important step forward to investigate the involvement of arabinogalactan in processes including interaction with biologically important transport proteins.
    Keywords alpha-amylase ; Andrographis paniculata ; anion exchange chromatography ; arabinogalactans ; bovine serum albumin ; dose response ; leaves ; methylation ; nuclear magnetic resonance spectroscopy ; phenolic acids ; transport proteins
    Language English
    Dates of publication 2014-0130
    Size p. 342-348.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1501516-6
    ISSN 1879-1344 ; 0144-8617
    ISSN (online) 1879-1344
    ISSN 0144-8617
    DOI 10.1016/j.carbpol.2013.09.022
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  7. Article: Antitussive arabinogalactan of Andrographis paniculata demonstrates synergistic effect with andrographolide

    Nosáľová, Gabriela / Ľudovít Jureček / Bimalendu Ray / Kanika Ghosh / Sujay Kumar Majee / Udipta Ranjan Chatterjee / Washim Raja

    International journal of biological macromolecules. 2014 Aug., v. 69

    2014  

    Abstract: Traditional Indian medicines have been used in humans for thousands of years. While the link to a particular indication has been established in man, the active principle of the formulations often remains unknown. In this study, we aim to investigate the ... ...

    Abstract Traditional Indian medicines have been used in humans for thousands of years. While the link to a particular indication has been established in man, the active principle of the formulations often remains unknown. In this study, we aim to investigate the structural features and antitussive activity of fractions from Andrographis paniculata leaves. In vivo investigations of water extract (WE), and both ethanol-soluble (WES) and precipitated (WEP) fractions from WE on the citric-acid induced cough efforts and airways smooth muscle reactivity in guinea pigs were performed. Chemical, chromatographic and spectroscopic analysis revealed the existence of a highly branched pectic arabinogalactan (109kDa) in WEP and andrographolide in WES. WEP showed significant antitussive activity while the potencies of WE and WES are even higher. Neither WE nor WES significantly alter specific airway smooth muscle reactivity. Remarkably, the antitussive activity of arabinogalactan could be increased by synergistic action with andrographolide. Finally, traditional aqueous extraction method provides an arabinogalactan from A. paniculata, which stimulate biological response but without addiction.
    Keywords Andrographis paniculata ; antitussive agents ; arabinogalactans ; chromatography ; citric acid ; cough ; guinea pigs ; humans ; leaves ; smooth muscle ; spectroscopy ; synergism
    Language English
    Dates of publication 2014-08
    Size p. 151-157.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2014.05.030
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