LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 2 of total 2

Search options

  1. Article: Novel Tools for Lassa Virus Surveillance in Peri-domestic Rodents.

    Smither, Allison R / Koninga, James / Kanneh, Franklyn B / Foday, Momoh / Boisen, Matthew L / Bond, Nell G / Momoh, Mambu / Sandi, John Demby / Kanneh, Lansana / Alhasan, Foday / Kanneh, Ibrahim Mustapha / Yillah, Mohamed S / Grant, Donald S / Bush, Duane J / Nelson, Diana K S / Cruz, Kaitlin M / Klitting, Raphaëlle / Pauthner, Matthias / Andersen, Kristian G /
    Shaffer, Jeffrey G / Cross, Robert W / Schieffelin, John S / Garry, Robert F

    medRxiv : the preprint server for health sciences

    2023  

    Abstract: Background: Lassa fever (LF) is a rodent-borne disease endemic to West Africa. In the absence of licensed therapeutics or vaccines, rodent exclusion from living spaces remains the primary method of preventing LF. Zoonotic surveillance of Lassa virus ( ... ...

    Abstract Background: Lassa fever (LF) is a rodent-borne disease endemic to West Africa. In the absence of licensed therapeutics or vaccines, rodent exclusion from living spaces remains the primary method of preventing LF. Zoonotic surveillance of Lassa virus (LASV), the etiologic agent of LF, can assess the burden of LASV in a region and guide public health measures against LF.
    Methods: In this study, we adapted commercially available LASV human diagnostics to assess the prevalence of LASV in peri-domestic rodents in Eastern Sierra Leone. Small mammal trapping was conducted in Kenema district, Sierra Leone between November 2018-July 2019. LASV antigen was detected using a commercially available LASV NP antigen rapid diagnostic test. LASV IgG antibodies against LASV nucleoprotein (NP) and glycoprotein (GP) were tested by adapting a commercially available semi-quantitative enzyme linked immunosorbent assay (ELISA) for detection of mouse-related and rat-related species IgG.
    Findings: Of the 373 tested specimens, 74 (20%) tested positive for LASV antigen. 40 (11%) specimens tested positive for LASV NP IgG, while an additional 12 (3%) specimens only tested positive for LASV GP IgG. Simultaneous antigen presence and IgG antibody presence was linked in
    Interpretation: The tools developed in this study can aid in the generation of valuable public health data for rapid field assessment of LASV burden during outbreak investigations and general LASV surveillance.
    Funding: Funding for this work was supported by the National Institute of Allergy and Infectious Diseases National Institute of Health, Department of Health and Human Services under the following grants: International Collaboration in Infectious Disease Research on Lassa fever and Ebola - ICIDR - U19 AI115589, Consortium for Viral Systems Biology - CViSB - 5U19AI135995, West African Emerging Infectious Disease Research Center - WARN-ID - U01AI151812, West African Center for Emerging Infectious Diseases: U01AI151801.
    Language English
    Publishing date 2023-03-20
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.03.17.23287380
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: Seroprevalence of anti-Lassa Virus IgG antibodies in three districts of Sierra Leone: A cross-sectional, population-based study.

    Grant, Donald S / Engel, Emily J / Roberts Yerkes, Nicole / Kanneh, Lansana / Koninga, James / Gbakie, Michael A / Alhasan, Foday / Kanneh, Franklyn B / Kanneh, Ibrahim Mustapha / Kamara, Fatima K / Momoh, Mambu / Yillah, Mohamed S / Foday, Momoh / Okoli, Adaora / Zeoli, Ashley / Weldon, Caroline / Bishop, Christopher M / Zheng, Crystal / Hartnett, Jessica /
    Chao, Karissa / Shore, Kayla / Melnik, Lilia I / Mucci, Mallory / Bond, Nell G / Doyle, Philip / Yenni, Rachael / Podgorski, Rachel / Ficenec, Samuel C / Moses, Lina / Shaffer, Jeffrey G / Garry, Robert F / Schieffelin, John S

    PLoS neglected tropical diseases

    2023  Volume 17, Issue 2, Page(s) e0010938

    Abstract: Background: Lassa virus (LASV), the cause of the acute viral hemorrhagic illness Lassa fever (LF), is endemic in West Africa. Infections in humans occur mainly after exposure to infected excrement or urine of the rodent-host, Mastomys natalensis. The ... ...

    Abstract Background: Lassa virus (LASV), the cause of the acute viral hemorrhagic illness Lassa fever (LF), is endemic in West Africa. Infections in humans occur mainly after exposure to infected excrement or urine of the rodent-host, Mastomys natalensis. The prevalence of exposure to LASV in Sierra Leone is crudely estimated and largely unknown. This cross-sectional study aimed to establish a baseline point seroprevalence of IgG antibodies to LASV in three administrative districts of Sierra Leone and identify potential risk factors for seropositivity and LASV exposure.
    Methodology and principal findings: Between 2015 and 2018, over 10,642 participants from Kenema, Tonkolili, and Port Loko Districts were enrolled in this cross-sectional study. Previous LASV and LF epidemiological studies support classification of these districts as "endemic," "emerging," and "non-endemic", respectively. Dried blood spot samples were tested for LASV antibodies by ELISA to determine the seropositivity of participants, indicating previous exposure to LASV. Surveys were administered to each participant to assess demographic and environmental factors associated with a higher risk of exposure to LASV. Overall seroprevalence for antibodies to LASV was 16.0%. In Kenema, Port Loko, and Tonkolili Districts, seroprevalences were 20.1%, 14.1%, and 10.6%, respectively. In a multivariate analysis, individuals were more likely to be LASV seropositive if they were living in Kenema District, regardless of sex, age, or occupation. Environmental factors contributed to an increased risk of LASV exposure, including poor housing construction and proximity to bushland, forested areas, and refuse.
    Conclusions and significance: In this study we determine a baseline LASV seroprevalence in three districts which will inform future epidemiological, ecological, and clinical studies on LF and the LASV in Sierra Leone. The heterogeneity of the distribution of LASV and LF over both space, and time, can make the design of efficacy trials and intervention programs difficult. Having more studies on the prevalence of LASV and identifying potential hyper-endemic areas will greatly increase the awareness of LF and improve targeted control programs related to LASV.
    MeSH term(s) Animals ; Humans ; Sierra Leone/epidemiology ; Cross-Sectional Studies ; Seroepidemiologic Studies ; Lassa Fever/epidemiology ; Lassa virus ; Virus Diseases ; Murinae ; Antibodies, Viral ; Immunoglobulin G
    Chemical Substances Antibodies, Viral ; Immunoglobulin G
    Language English
    Publishing date 2023-02-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2429704-5
    ISSN 1935-2735 ; 1935-2735
    ISSN (online) 1935-2735
    ISSN 1935-2735
    DOI 10.1371/journal.pntd.0010938
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top