Article ; Online: Comparison of the measured values of quantitative SARS-CoV-2 spike antibody assays.
2022 Volume 155, Page(s) 105269
Abstract: Background: The concentration of antibodies against the SARS-CoV-2 spike protein is frequently being measured for clinical and epidemiological purposes. The aim of this study was to examine whether the results of different quantitative SARS-CoV-2 spike ... ...
| Abstract | Background: The concentration of antibodies against the SARS-CoV-2 spike protein is frequently being measured for clinical and epidemiological purposes. The aim of this study was to examine whether the results of different quantitative SARS-CoV-2 spike antibody assays are comparable. Material and methods: The Siemens SARS-CoV-2 IgG, Abbott SARS-CoV-2 IgG II Quant, Roche ElecsysT Anti-SARS-CoV-2 S, and Euroimmun Anti-SARS-CoV-2-QuantiVac assay were compared with 110 sera from patients 6-9 months after SARS-CoV-2 infection and the WHO First International SARS-CoV-2 antibody standard 20/136. The antibody values were converted into WHO binding antibody units (BAU)/ml. The diagnostic sensitivity of the assays was determined and the antibody values were compared. Results: The diagnostic sensitivity ranged from 57.3% (Euroimmun) to 100% (Roche). The antibody concentration values of different assays correlated with Pearson coefficients of correlation between 0.729 and 0.953. The geometric mean antibody values of the Abbott, Siemens and Euroimmun assay varied by a factor of 1.1-1.2. The geometric mean antibody values of the Roche assay were 2.4-2.8 times higher than those from the other assays. The assays yielded varying results with the WHO International antibody standard. Conclusions: The quantitative SARS-CoV-2 antibody assays from Abbott, Siemens, Roche and Euroimmun correlate strongly but differ in the antibody concentrations. Therefore, the same assay should be used when testing patients repeatedly. In addition, the name of the assay used and the manufacturer should be indicated along with the test results. |
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| MeSH term(s) | Antibodies, Viral ; COVID-19/diagnosis ; Humans ; Immunoglobulin G ; SARS-CoV-2 ; Sensitivity and Specificity ; Spike Glycoprotein, Coronavirus/analysis ; Spike Glycoprotein, Coronavirus/chemistry |
| Chemical Substances | Antibodies, Viral ; Immunoglobulin G ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 |
| Language | English |
| Publishing date | 2022-08-19 |
| Publishing country | Netherlands |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 1446080-4 |
| ISSN | 1873-5967 ; 1386-6532 |
| ISSN (online) | 1873-5967 |
| ISSN | 1386-6532 |
| DOI | 10.1016/j.jcv.2022.105269 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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