LIVIVO - Search results -

Search results

Result 1 - 10 of total 20

Search options

Article: Recipe for pituitary organoids.

Kano, Mayuko / Sasaki, Hiroo / Miwata, Tsutomu / Suga, Hidetaka

2023 Volume 13, Page(s) 1025825

Abstract: Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been ... ...

Abstract	Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been attempted
MeSH term(s)	Animals ; Humans ; Mice ; Cell Differentiation ; Hedgehog Proteins ; Hypopituitarism ; Organoids ; Pituitary Gland
Chemical Substances	Hedgehog Proteins
Language	English
Publishing date	2023-01-19
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2592084-4
ISSN	1664-2392
ISSN	1664-2392
DOI	10.3389/fendo.2022.1025825
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Xenotransplantation and interspecies organogenesis: current status and issues.

Kano, Mayuko / Mizutani, Eiji / Homma, Shota / Masaki, Hideki / Nakauchi, Hiromitsu

Frontiers in endocrinology

2022 Volume 13, Page(s) 963282

Abstract: Pancreas (and islet) transplantation is the only curative treatment for type 1 diabetes patients whose β-cell functions have been abolished. However, the lack of donor organs has been the major hurdle to save a large number of patients. Therefore, ... ...

Abstract	Pancreas (and islet) transplantation is the only curative treatment for type 1 diabetes patients whose β-cell functions have been abolished. However, the lack of donor organs has been the major hurdle to save a large number of patients. Therefore, transplantation of animal organs is expected to be an alternative method to solve the serious shortage of donor organs. More recently, a method to generate organs from pluripotent stem cells inside the body of other species has been developed. This interspecies organ generation using blastocyst complementation (BC) is expected to be the next-generation regenerative medicine. Here, we describe the recent advances and future prospects for these two approaches.
MeSH term(s)	Animals ; Blastocyst ; Organogenesis/physiology ; Pluripotent Stem Cells ; Regenerative Medicine ; Transplantation, Heterologous
Language	English
Publishing date	2022-08-05
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2592084-4
ISSN	1664-2392
ISSN	1664-2392
DOI	10.3389/fendo.2022.963282
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Induction of Functional Hypothalamus and Pituitary Tissues From Pluripotent Stem Cells for Regenerative Medicine.

Kano, Mayuko / Suga, Hidetaka / Arima, Hiroshi

Journal of the Endocrine Society

2020 Volume 5, Issue 3, Page(s) bvaa188

Abstract: The hypothalamus and pituitary have been identified to play essential roles in maintaining homeostasis. Various diseases can disrupt the functions of these systems, which can often result in serious lifelong symptoms. The current treatment for ... ...

Abstract	The hypothalamus and pituitary have been identified to play essential roles in maintaining homeostasis. Various diseases can disrupt the functions of these systems, which can often result in serious lifelong symptoms. The current treatment for hypopituitarism involves hormone replacement therapy. However, exogenous drug administration cannot mimic the physiological changes that are a result of hormone requirements. Therefore, patients are at a high risk of severe hormone deficiency, including adrenal crisis. Pluripotent stem cells (PSCs) self-proliferate and differentiate into all types of cells. The generation of endocrine tissues from PSCs has been considered as another new treatment for hypopituitarism. Our colleagues established a 3-dimensional (3D) culture method for embryonic stem cells (ESCs). In this culture, the ESC-derived aggregates exhibit self-organization and spontaneous formation of highly ordered patterning. Recent results have shown that strict removal of exogenous patterning factors during early differentiation efficiently induces rostral hypothalamic progenitors from mouse ESCs. These hypothalamic progenitors generate vasopressinergic neurons, which release neuropeptides upon exogenous stimulation. Subsequently, we reported adenohypophysis tissue self-formation in 3D cultures of mouse ESCs. The ESCs were found to differentiate into both nonneural oral ectoderm and hypothalamic neuroectoderm in adjacent layers. Interactions between the 2 tissues appear to be critically important for in vitro induction of a Rathke's pouch-like developing embryo. Various endocrine cells were differentiated from nonneural ectoderm. The induced corticotrophs efficiently secreted adrenocorticotropic hormone when engrafted in vivo, which rescued hypopituitary hosts. For future regenerative medicine, generation of hypothalamic and pituitary tissues from human PSCs is necessary. We and other groups succeeded in establishing a differentiation method with the use of human PSCs. Researchers could use these methods for models of human diseases to elucidate disease pathology or screen potential therapeutics.
Language	English
Publishing date	2020-12-02
Publishing country	United States
Document type	Journal Article ; Review
ISSN	2472-1972
ISSN (online)	2472-1972
DOI	10.1210/jendso/bvaa188
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Skin graft with dermis and appendages generated in vivo by cell competition.

Nagano, Hisato / Mizuno, Naoaki / Sato, Hideyuki / Mizutani, Eiji / Yanagida, Ayaka / Kano, Mayuko / Kasai, Mariko / Yamamoto, Hiromi / Watanabe, Motoo / Suchy, Fabian / Masaki, Hideki / Nakauchi, Hiromitsu

Nature communications

2024 Volume 15, Issue 1, Page(s) 3366

Abstract: Autologous skin grafting is a standard treatment for skin defects such as burns. No artificial skin substitutes are functionally equivalent to autologous skin grafts. The cultured epidermis lacks the dermis and does not engraft deep wounds. Although ... ...

Abstract	Autologous skin grafting is a standard treatment for skin defects such as burns. No artificial skin substitutes are functionally equivalent to autologous skin grafts. The cultured epidermis lacks the dermis and does not engraft deep wounds. Although reconstituted skin, which consists of cultured epidermal cells on a synthetic dermal substitute, can engraft deep wounds, it requires the wound bed to be well-vascularized and lacks skin appendages. In this study, we successfully generate complete skin grafts with pluripotent stem cell-derived epidermis with appendages on p63 knockout embryos' dermis. Donor pluripotent stem cell-derived keratinocytes encroach the embryos' dermis by eliminating p63 knockout keratinocytes based on cell-extracellular matrix adhesion mediated cell competition. Although the chimeric skin contains allogenic dermis, it is engraftable as long as autologous grafts. Furthermore, we could generate semi-humanized skin segments by human keratinocytes injection into the amnionic cavity of p63 knockout mice embryos. Niche encroachment opens the possibility of human skin graft production in livestock animals.
MeSH term(s)	Animals ; Skin Transplantation/methods ; Keratinocytes/cytology ; Keratinocytes/transplantation ; Humans ; Dermis/cytology ; Dermis/transplantation ; Mice, Knockout ; Mice ; Epidermis/metabolism ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/transplantation ; Skin, Artificial ; Epidermal Cells/transplantation ; Epidermal Cells/cytology ; Extracellular Matrix/metabolism ; Skin/cytology
Language	English
Publishing date	2024-04-29
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-47527-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Association Between Caregiver's Perception of "Good" Dietary Habits and Food Group Intake Among Preschool Children in Tokyo, Japan.

Kano, Mayuko / Tani, Yukako / Ochi, Manami / Sudo, Noriko / Fujiwara, Takeo

Frontiers in pediatrics

2020 Volume 7, Page(s) 554

Abstract: Given that parents are mainly responsible for a preschooler's dietary management, they need to understand a child's diet. However, few studies have examined the association between parental perception of a preschool child's "good" dietary habits and ... ...

Abstract	Given that parents are mainly responsible for a preschooler's dietary management, they need to understand a child's diet. However, few studies have examined the association between parental perception of a preschool child's "good" dietary habits and actual food intake. We conducted a cross-sectional study investigating whether a child's food intake would differ depending on the caregiver's perception of their child's dietary habits among 4-year-old nursery school children at Adachi City, Tokyo, Japan. Children's dietary data were collected using the brief-type self-administered diet history questionnaire for children Aged 3-6 Years (BDHQ3y), while caregivers' perceptions of their child's dietary habits (good, normal, and poor) were inquired (
Language	English
Publishing date	2020-01-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2711999-3
ISSN	2296-2360
ISSN	2296-2360
DOI	10.3389/fped.2019.00554
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Functional calcium-responsive parathyroid glands generated using single-step blastocyst complementation.

Kano, Mayuko / Mizuno, Naoaki / Sato, Hideyuki / Kimura, Takaharu / Hirochika, Rei / Iwasaki, Yasumasa / Inoshita, Naoko / Nagano, Hisato / Kasai, Mariko / Yamamoto, Hiromi / Yamaguchi, Tomoyuki / Suga, Hidetaka / Masaki, Hideki / Mizutani, Eiji / Nakauchi, Hiromitsu

Proceedings of the National Academy of Sciences of the United States of America

2023 Volume 120, Issue 28, Page(s) e2216564120

Abstract: Patients with permanent hypoparathyroidism require lifelong replacement therapy to avoid life-threatening complications, The benefits of conventional treatment are limited, however. Transplanting a functional parathyroid gland (PTG) would yield better ... ...

Abstract	Patients with permanent hypoparathyroidism require lifelong replacement therapy to avoid life-threatening complications, The benefits of conventional treatment are limited, however. Transplanting a functional parathyroid gland (PTG) would yield better results. Parathyroid gland cells generated from pluripotent stem cells in vitro to date cannot mimic the physiological responses to extracellular calcium that are essential for calcium homeostasis. We thus hypothesized that blastocyst complementation (BC) could be a better strategy for generating functional PTG cells and compensating loss of parathyroid function. We here describe generation of fully functional PTGs from mouse embryonic stem cells (mESCs) with single-step BC. Using CRISPR-Cas9 knockout of
MeSH term(s)	Humans ; Animals ; Mice ; Rats ; Parathyroid Glands ; Calcium ; Hypoparathyroidism/genetics ; Hypoparathyroidism/therapy ; Calcium, Dietary ; Blastocyst
Chemical Substances	Calcium (SY7Q814VUP) ; Calcium, Dietary
Language	English
Publishing date	2023-06-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.2216564120
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1148: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Functional Pituitary Tissue Generation from Human Embryonic Stem Cells.

Kano, Mayuko / Suga, Hidetaka / Kasai, Takatoshi / Ozone, Chikafumi / Arima, Hiroshi

Current protocols in neuroscience

2018 Volume 83, Issue 1, Page(s) e48

Abstract: The anterior pituitary gland produces several hormones essential for regulation of the somatic endocrine system. Deficiency of these hormones can cause life-threatening diseases, including adrenal crisis. Pituitary tissue generated from human pluripotent ...

Abstract	The anterior pituitary gland produces several hormones essential for regulation of the somatic endocrine system. Deficiency of these hormones can cause life-threatening diseases, including adrenal crisis. Pituitary tissue generated from human pluripotent stem cells is expected to provide better treatment than current hormone replacement therapy. During early mammalian development, the pituitary anlage (Rathke's pouch) develops from non-neural ectoderm adjacent to the developing ventral hypothalamus. The close interaction between these two tissues is crucial for Rathke's pouch development and involves several signaling molecules. Early exposure of human embryonic stem cells in 3D floating culture to sonic hedgehog and bone morphogenetic protein 4 promoted the cells' differentiation into oral ectoderm and, subsequently, hormone-producing cells such as corticotrophs (adrenocorticotropic hormone-producing cells). The differentiation approach described herein, which induces the formation of pituitary tissue in contact with hypothalamic neural tissue, mimics mammalian pituitary development. The differentiated corticotrophs are functional, responding normally to both release and feedback signals. © 2018 by John Wiley & Sons, Inc.
MeSH term(s)	Cell Culture Techniques/methods ; Cell Differentiation/physiology ; Embryonic Development/physiology ; Human Embryonic Stem Cells/pathology ; Humans ; Pituitary Gland/pathology ; Pluripotent Stem Cells/pathology
Language	English
Publishing date	2018-07-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1934-8576
ISSN (online)	1934-8576
DOI	10.1002/cpns.48
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Investigation of Staphylococcus aureus positive for Staphylococcal enterotoxin S and T genes.

Sato'o, Yusuke / Omoe, Katsuhiko / Aikawa, Yasuko / Kano, Mayuko / Ono, Hisaya K / Hu, Dong-Liang / Nakane, Akio / Sugai, Motoyuki

The Journal of veterinary medical science

2021 Volume 83, Issue 7, Page(s) 1120–1127

Abstract: Staphylococcus aureus produces staphylococcal enterotoxins (SEs) and causes food poisoning. It is known that almost all SE-encoding genes are present on various types of mobile genetic elements and can mobilize among S. aureus populations. Further, ... ...

Abstract	Staphylococcus aureus produces staphylococcal enterotoxins (SEs) and causes food poisoning. It is known that almost all SE-encoding genes are present on various types of mobile genetic elements and can mobilize among S. aureus populations. Further, plasmids comprise one of SE gene carriers. Previously, we reported novel SEs, SES and SET, harbored by the plasmid pF5 from Fukuoka5. In the present study, we analyzed the distribution of these SEs in various S. aureus isolates in Japan. We used 526 S. aureus strains and found 311 strains positive for at least one SE/SE-like toxin gene, but only two strains (Fukuoka5 and Hiroshima3) were positive for ses and set among the specimens. We analyzed two plasmids (pF5 and pH3) from these strains and found that they were different. Whereas these plasmids partially shared similar sequences involved in the ser/selj/set/ses gene cluster, other sequences were different. A comparison of these plasmids with those deposited in the NCBI database revealed that only one plasmid had the ser/selj/set/ses cluster with a stop mutation in set similar to that in pH3. In addition, the chromosomes of Fukuoka5 and Hiroshima3, positive for ses and set, were classified into different genotypes. Despite the low rate of gene positivity for these SEs, it is suggested that there is diversity in plasmids and strains carrying these two SEs. Consequently, regarding the entire feature of SE prevalence, we improved the multiplex PCR detection method for the SE superfamily to obtain further insight.
MeSH term(s)	Animals ; Enterotoxins/genetics ; Food Microbiology ; Japan/epidemiology ; Staphylococcal Food Poisoning/veterinary ; Staphylococcal Infections/epidemiology ; Staphylococcal Infections/veterinary ; Staphylococcus aureus/genetics
Chemical Substances	Enterotoxins
Language	English
Publishing date	2021-05-26
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1071753-5
ISSN	1347-7439 ; 0916-7250
ISSN (online)	1347-7439
ISSN	0916-7250
DOI	10.1292/jvms.20-0662
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 579: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Generation of hypothalamic neural stem cell-like cells in vitro from human pluripotent stem cells.

Miwata, Tsutomu / Suga, Hidetaka / Kawaguchi, Yohei / Sakakibara, Mayu / Kano, Mayuko / Taga, Shiori / Soen, Mika / Ozaki, Hajime / Asano, Tomoyoshi / Sasaki, Hiroo / Miyata, Takashi / Yasuda, Yoshinori / Kobayashi, Tomoko / Sugiyama, Mariko / Onoue, Takeshi / Takagi, Hiroshi / Hagiwara, Daisuke / Iwama, Shintaro / Arima, Hiroshi

Stem cell reports

2023 Volume 18, Issue 4, Page(s) 869–883

Abstract: When damaged, restoring the function of the hypothalamus is currently impossible. It is unclear whether neural stem cells exist in the hypothalamus. Studies have reported that adult rodent tanycytes around the third ventricle function as hypothalamic ... ...

Abstract	When damaged, restoring the function of the hypothalamus is currently impossible. It is unclear whether neural stem cells exist in the hypothalamus. Studies have reported that adult rodent tanycytes around the third ventricle function as hypothalamic neural stem cell-like cells. However, it is currently impossible to collect periventricular cells from humans. We attempted to generate hypothalamic neural stem cell-like cells from human embryonic stem cells (ESCs). We focused on retina and anterior neural fold homeobox (RAX) because its expression is gradually restricted to tanycytes during the late embryonic stage. We differentiated RAX::VENUS knockin human ESCs (hESCs) into hypothalamic organoids and sorted RAX
MeSH term(s)	Mice ; Animals ; Humans ; Neural Stem Cells ; Cell Differentiation/physiology ; Pluripotent Stem Cells ; Neurogenesis/physiology ; Hypothalamus/metabolism
Language	English
Publishing date	2023-03-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2720528-9
ISSN	2213-6711 ; 2213-6711
ISSN (online)	2213-6711
ISSN	2213-6711
DOI	10.1016/j.stemcr.2023.02.006
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Differentiation of human induced pluripotent stem cells into hypothalamic vasopressin neurons with minimal exogenous signals and partial conversion to the naive state.

Scientific reports

2022 Volume 12, Issue 1, Page(s) 17381

Abstract: Familial neurohypophyseal diabetes insipidus (FNDI) is a degenerative disease of vasopressin (AVP) neurons. Studies in mouse in vivo models indicate that accumulation of mutant AVP prehormone is associated with FNDI pathology. However, studying human ... ...

Abstract	Familial neurohypophyseal diabetes insipidus (FNDI) is a degenerative disease of vasopressin (AVP) neurons. Studies in mouse in vivo models indicate that accumulation of mutant AVP prehormone is associated with FNDI pathology. However, studying human FNDI pathology in vivo is technically challenging. Therefore, an in vitro human model needs to be developed. When exogenous signals are minimized in the early phase of differentiation in vitro, mouse embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs) differentiate into AVP neurons, whereas human ESCs/iPSCs die. Human ESCs/iPSCs are generally more similar to mouse epiblast stem cells (mEpiSCs) compared to mouse ESCs. In this study, we converted human FNDI-specific iPSCs by the naive conversion kit. Although the conversion was partial, we found improved cell survival under minimal exogenous signals and differentiation into rostral hypothalamic organoids. Overall, this method provides a simple and straightforward differentiation direction, which may improve the efficiency of hypothalamic differentiation.
MeSH term(s)	Animals ; Cell Differentiation ; Diabetes Insipidus, Neurogenic ; Humans ; Hypothalamus/metabolism ; Induced Pluripotent Stem Cells/metabolism ; Mice ; Neurons/metabolism ; Vasopressins/metabolism
Chemical Substances	Vasopressins (11000-17-2)
Language	English
Publishing date	2022-10-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-22405-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito