Article ; Online: Predicted aggregation-prone region (APR) in βB1-crystallin forms the amyloid-like structure and induces aggregation of soluble proteins isolated from human cataractous eye lens.
International journal of biological macromolecules
2020 Volume 163, Page(s) 702–710
Abstract: The aggregation of β-crystallins in the human eye lens constitutes a critical step during the development of cataract. We anticipated that the presence of Aggregation-Prone Regions (APRs) in their primary structure, which might be responsible for ... ...
| Abstract | The aggregation of β-crystallins in the human eye lens constitutes a critical step during the development of cataract. We anticipated that the presence of Aggregation-Prone Regions (APRs) in their primary structure, which might be responsible for conformational change required for the self-assembly. To examine the presence of APRs, we systematically analyzed the primary structures of β-crystallins. Out of seven subtypes, the βB1-crystallin found to possess the highest aggregation score with 9 APRs in its primary structure. To confirm the amyloidogenic nature of these newly identified APRs, we further studied the aggregation behavior of one of the APRs spanning from 174 to 180 residues ( |
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| MeSH term(s) | Adsorption ; Amyloidogenic Proteins/chemistry ; Amyloidogenic Proteins/isolation & purification ; Amyloidogenic Proteins/metabolism ; Amyloidogenic Proteins/ultrastructure ; Amyloidosis ; Cataract/metabolism ; Chemical Phenomena ; Congo Red/chemistry ; Lens, Crystalline/chemistry ; Lens, Crystalline/metabolism ; Molecular Dynamics Simulation ; Protein Aggregates ; Protein Conformation ; Solubility ; Structure-Activity Relationship ; beta-Crystallin B Chain/chemistry ; beta-Crystallin B Chain/metabolism |
| Chemical Substances | Amyloidogenic Proteins ; Protein Aggregates ; beta-Crystallin B Chain ; Congo Red (3U05FHG59S) |
| Language | English |
| Publishing date | 2020-07-08 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 282732-3 |
| ISSN | 1879-0003 ; 0141-8130 |
| ISSN (online) | 1879-0003 |
| ISSN | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2020.07.028 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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