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Article: Digital Skin Ulceration and Acro-Osteolysis: A Rare Presentation of Severe Carpal Tunnel Syndrome.

Zorlu, Ozge / Karabağ, Sevil

2022 Volume 13, Issue 6, Page(s) 798–799

Language	English
Publishing date	2022-07-18
Publishing country	India
Document type	Journal Article
ZDB-ID	2585814-2
ISSN	2249-5673 ; 2229-5178
ISSN (online)	2249-5673
ISSN	2229-5178
DOI	10.4103/idoj.idoj_92_22
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Article ; Online: Histopathological and histomorphometric investigation of the effects of different irrigation solutions on Achilles tendon healing in rats.

Dincel, Yaşar Mahsut / Can, Erdem / Karabag, Sevil

Joint diseases and related surgery

2023 Volume 35, Issue 1, Page(s) 186–193

Abstract: Objectives: This study aimed to examine the effect of irrigation fluids containing povidone-iodine (PVP-I), rifampicin (RF), and chlorhexidine gluconate (CHG) used during surgery on healing on a rat Achilles tendon model.: Materials and methods: ... ...

Abstract	Objectives: This study aimed to examine the effect of irrigation fluids containing povidone-iodine (PVP-I), rifampicin (RF), and chlorhexidine gluconate (CHG) used during surgery on healing on a rat Achilles tendon model. Materials and methods: Twenty-eight male Sprague-Dawley rats (range, 300 to 400 g) were used in the experiment carried out between November 2022 and December 2022. The rats were divided into PVP-I, RF, CHG, and control groups, with seven rats in each group. Following the tenotomy and repair of the right Achilles tendon, the surgical site was irrigated using PVP-I, RF, CHG, or normal saline (the control group) for 2 min. All rats were sacrificed on the 21 Results: The RF group gave better results in all three scoring systems compared to the control, PVP-I, and CHG groups (p=0.008, p=0.002, and p=0.006, respectively). Cellularity, rounding, and tenocyte morphology showed a significant difference in favor of the RF group (p=0.004). While the distribution of ground substance glycosaminoglycans showed a significant difference in favor of the RF group, there was no significant difference among the other groups (p=0.22). Conclusion: Irrigation solutions containing PVP-I, RF, or CHG show no negative effect on Achilles tendon healing. Moreover, the findings suggest that RF irrigation can accelerate the healing process.
MeSH term(s)	Rats ; Male ; Animals ; Povidone-Iodine/pharmacology ; Rats, Sprague-Dawley ; Achilles Tendon/surgery ; Achilles Tendon/pathology ; Chlorhexidine/pharmacology
Chemical Substances	Povidone-Iodine (85H0HZU99M) ; chlorhexidine gluconate (MOR84MUD8E) ; Chlorhexidine (R4KO0DY52L)
Language	English
Publishing date	2023-11-30
Publishing country	Turkey
Document type	Journal Article
ISSN	2687-4792
ISSN (online)	2687-4792
DOI	10.52312/jdrs.2023.1342
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Article ; Online: The effects of etomidate on testicular ischemia reperfusion injury in ipsilateral and contralateral testes of rats.

Jafarova Demirkapu, M / Karabag, S / Akgul, H M / Mordeniz, C / Yananli, H R

European review for medical and pharmacological sciences

2022 Volume 26, Issue 1, Page(s) 211–217

Abstract: Objective: Testicular torsion is a condition that manifests with acute pain and can lead to infertility despite urgent surgical intervention. The aim of this study is to investigate the protective and preventive effects of etomidate, an imidazole ... ...

Abstract	Objective: Testicular torsion is a condition that manifests with acute pain and can lead to infertility despite urgent surgical intervention. The aim of this study is to investigate the protective and preventive effects of etomidate, an imidazole derivative, a non-barbiturate general anesthetic agent, without analgesic effect, on testicular ischemia-reperfusion injury. Materials and methods: Twenty-four adult male Wistar rats weighing 200-250 g were used in the study. Torsion was created in all rats by rotating left testes 720° clockwise on the day of the experiment. 30 minutes before detorsion, 4 mg/kg etomidate and 10 mg/kg propofol were administered intraperitoneally to the etomidate and propofol groups, respectively. After an hour of ischemia, the left testis was reinstated, and the tissues were repaired according to their physiology. Following 24 hours of reperfusion, the animals were euthanized after ipsilateral and contralateral testes were removed. Results: Etomidate applied before testicular detorsion significantly suppressed germ cell damage and Leydig cell loss in ipsilateral tissue. It did not cause any significant changes in the percentage of necrosis, histological score, and tubule rupture in ipsilateral tissue. Propofol administered before testicular detorsion significantly suppressed the percentage of necrosis only in the ipsilateral tissue. In addition, no signs of damage were observed in the contralateral testis. Conclusions: These findings show that etomidate administered before detortion creates a protective effect by preventing testicular ischemia-reperfusion injury.
MeSH term(s)	Animals ; Etomidate/pharmacology ; Male ; Malondialdehyde ; Rats ; Rats, Wistar ; Reperfusion Injury/drug therapy ; Reperfusion Injury/pathology ; Reperfusion Injury/prevention & control ; Testis
Chemical Substances	Malondialdehyde (4Y8F71G49Q) ; Etomidate (Z22628B598)
Language	English
Publishing date	2022-01-20
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_202201_27770
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Article ; Online: Effect of HER2 and Fascin expression on muscle-invasive bladder cancers: Classification by basaloid and luminal phenotypes.

Arslan, Ayşegül I / Karabağ, Sevil / Akgül, Murat

Indian journal of pathology & microbiology

2022 Volume 65, Issue 3, Page(s) 604–609

Abstract: Purpose: The present study aims to identify basaloid and luminal molecular groups and the p53-like sub-group, which is a sub-group of the luminal group, using a specific immunohistochemical panel and investigate human epithelial growth factor receptor 2 ...

Abstract	Purpose: The present study aims to identify basaloid and luminal molecular groups and the p53-like sub-group, which is a sub-group of the luminal group, using a specific immunohistochemical panel and investigate human epithelial growth factor receptor 2 (HER2)/Neu and Fascin expression in these groups to analyze their relationship with clinicopathological features and prognosis in a cohort of cases with muscle-invasive urothelial bladder carcinoma (MIBC). Material and methods: An immunohistochemical panel that included GATA-3, CK20, CD44, and CK5/6 was used to identify molecular sub-groups based on expression in 44 cases of MIBC. HER2/Neu and Fascin expression in basal, luminal, and p53-like groups and the relationship with clinicopathological features and prognosis were investigated. Results: The distribution of the molecular sub-groups determined by immunohistochemistry was as follows: 23 luminal cases (52.3%), 16 basal cases (36.4%), and 5 (11.4%) p53-like cases. There was a statistically significant difference in tumor size across the groups, with the greatest size in the p53-like group (p = 0.001). A statistically significant difference was observed in HER2/Neu expression between the molecular sub-groups (p = 0.017). Comparison of survival and HER2/Neu scores revealed shorter survival in patients with an HER2/Neu score of 3 + compared to those with scores of 0, 1+, and 2+ (p = 0.109). Fascin immunoreactivity was more common in the p53-like and basal groups compared to the luminal group (p = 0.036). Conclusion: Despite the limited number of cases in the MIBC group, our results support that HER2/Neu expression in the luminal sub-group and Fascin expression in basal and p53-like groups may be used as a negative prognostic marker. Multi-center studies that include large case series are warranted in this field.
MeSH term(s)	Biomarkers, Tumor/metabolism ; Breast Neoplasms ; Carrier Proteins ; Female ; Humans ; Microfilament Proteins ; Muscles/pathology ; Phenotype ; Prognosis ; Receptor, ErbB-2/genetics ; Receptor, ErbB-2/metabolism ; Tumor Suppressor Protein p53 ; Urinary Bladder Neoplasms/diagnosis ; Urinary Bladder Neoplasms/pathology
Chemical Substances	Biomarkers, Tumor ; Carrier Proteins ; Microfilament Proteins ; Tumor Suppressor Protein p53 ; fascin (146808-54-0) ; Receptor, ErbB-2 (EC 2.7.10.1)
Language	English
Publishing date	2022-07-28
Publishing country	India
Document type	Journal Article
ZDB-ID	197621-7
ISSN	0974-5130 ; 0377-4929
ISSN (online)	0974-5130
ISSN	0377-4929
DOI	10.4103/ijpm.ijpm_537_21
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Article ; Online: Histiocytic Sarcoma with Invasive Urothelial Carcinoma of Urinary Bladder.

Arslan, Aysegul Isal / Karabag, Sevil / Akgul, Murat

Journal of the College of Physicians and Surgeons--Pakistan : JCPSP

2022 Volume 32, Issue 4, Page(s) S79–S81

Abstract: We report a rare case of histiocytic sarcoma in association with invasive urothelial carcinoma involving the urinary bladder. A 67-year male patient, who presented with a complaint of macroscopic hematuria, was found to have a mass on urinary system ... ...

Abstract	We report a rare case of histiocytic sarcoma in association with invasive urothelial carcinoma involving the urinary bladder. A 67-year male patient, who presented with a complaint of macroscopic hematuria, was found to have a mass on urinary system ultrasonography. Abdominal magnetic resonance imaging was performed to evaluate the mass, which showed a 52×24 mm mass on the posterior wall of the bladder. The cystoscopic examination revealed two suspicious areas in close proximity to one another with solid-papillary character. The tissue samples were collected by means of transurethral resection. The evaluation of these samples revealed two distinct neoplastic patterns. The areas of invasive urothelial carcinoma infiltrating lamina propria were noted on the surface in addition to diffuse sheets of large cells with hyperchromatic nuclei and ample clear cytoplasm, with a patternless pattern among small lymphocytes in myxoid background in the lamina propria. The positive reactions were observed in these areas with CD45, fascin, and CD68, a histiocytic marker. The histopathological diagnosis was histiocytic sarcoma in combination with invasive urothelial carcinoma. Histiocytic sarcoma may mimic several other malignant lesions, and only immunohistochemistry can identify this tumour to allow correct treatment. We present this rare case to emphasise that this phenomenon should be considered in unusual tumors and sites. Key Words: Urinary bladder, Histiocytic sarcoma, Invasive urothelial carcinoma.
MeSH term(s)	Carcinoma, Transitional Cell/diagnostic imaging ; Carcinoma, Transitional Cell/pathology ; Carcinoma, Transitional Cell/surgery ; Cystectomy ; Histiocytic Sarcoma/diagnosis ; Histiocytic Sarcoma/pathology ; Histiocytic Sarcoma/surgery ; Humans ; Male ; Urinary Bladder/diagnostic imaging ; Urinary Bladder/pathology ; Urinary Bladder/surgery ; Urinary Bladder Neoplasms/diagnostic imaging ; Urinary Bladder Neoplasms/surgery
Language	English
Publishing date	2022-05-23
Publishing country	Pakistan
Document type	Case Reports
ZDB-ID	2276646-7
ISSN	1681-7168 ; 1022-386X
ISSN (online)	1681-7168
ISSN	1022-386X
DOI	10.29271/jcpsp.2022.Supp1.S79
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Article ; Online: Relapse of minimal change disease after inactivated SARS-CoV-2 vaccination: case report.

Özkan, Gülsüm / Bayrakçı, N / Karabağ, S / Güzel, E Ç / Ulusoy, S

International urology and nephrology

2021 Volume 54, Issue 4, Page(s) 971–972

MeSH term(s)	COVID-19/prevention & control ; COVID-19 Vaccines/adverse effects ; Humans ; Nephrosis, Lipoid ; Recurrence ; SARS-CoV-2 ; Vaccination
Chemical Substances	COVID-19 Vaccines
Language	English
Publishing date	2021-06-28
Publishing country	Netherlands
Document type	Case Reports ; Letter
ZDB-ID	204048-7
ISSN	1573-2584 ; 0301-1623 ; 0042-1162
ISSN (online)	1573-2584
ISSN	0301-1623 ; 0042-1162
DOI	10.1007/s11255-021-02889-5
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Article ; Online: Histopathological and immunohistochemical investigation of the local and systemic effects of tranexamic acid on the healing of the Achilles tendon in rats.

Sarı, Abdulkadir / Dinçel, Yaşar Mahsut / Karabağ, Sevil / Çetin, Mehmet Ümit

Joint diseases and related surgery

2021 Volume 32, Issue 1, Page(s) 152–161

Abstract: Objectives: This study aims to compare the effects of systemic and local applications of tranexamic acid (TXA) on tendon healing using a rat Achilles tendon injury model.: Patients and methods: Thirty-six adult male albino Wistar rats (aging 3-4 ... ...

Abstract	Objectives: This study aims to compare the effects of systemic and local applications of tranexamic acid (TXA) on tendon healing using a rat Achilles tendon injury model. Patients and methods: Thirty-six adult male albino Wistar rats (aging 3-4 months; weighing 350 to 400 g) were used in this study conducted between December 2019 and January 2020. The Achilles tendon was performed bilateral tenotomy and surgically repaired. Postoperatively, 1 mL of TXA was administered to each leg locally in the local group, whereas 2 mL of TXA was intraperitoneally administered in the systemic group. The control group was left untreated. Half of the rats were sacrificed on Day 15 and the other half on Day 30. Tendon healing was evaluated with the Bonar and the Movin scoring systems and immunohistochemical methods. Results: The systemic group had the highest Bonar and Movin scores on Day 15. All groups exhibited tendon healing on Day 30, with no significant differences among the groups. The tenocyte morphology was found to be more impaired in both TXA groups on Day 30 (p=0.013). Ground substance scores were lower in the systemic group on Day 30 (p=0.028). The fiber structure and arrangement scores were higher in the systemic group on Day 15 (p=0.007 and p=0.032). Immunohistochemical analyses showed that galectin-3 values exhibited a significant difference in all groups on Day 30 (p=0.020). In all groups, it was determined that type I collagen values showed an increasing trend on Day 30, compared to the values on Day 15, whereas type III collagen values showed a decreasing trend. Conclusion: Our results demonstrated that local and systemic use of TXA does not impair tendon healing. Although advanced studies are needed, our study suggests that TXA application reduces the development of fibrosis.
MeSH term(s)	Achilles Tendon/injuries ; Achilles Tendon/pathology ; Achilles Tendon/surgery ; Animals ; Antifibrinolytic Agents/pharmacology ; Collagen Type I/metabolism ; Collagen Type III/metabolism ; Disease Models, Animal ; Male ; Rats ; Rats, Wistar ; Tendon Injuries/surgery ; Tenocytes ; Tenotomy ; Tranexamic Acid/pharmacology ; Wound Healing/drug effects
Chemical Substances	Antifibrinolytic Agents ; Collagen Type I ; Collagen Type III ; Tranexamic Acid (6T84R30KC1)
Language	English
Publishing date	2021-01-06
Publishing country	Turkey
Document type	Journal Article
ISSN	2687-4792
ISSN (online)	2687-4792
DOI	10.5606/ehc.2021.76301
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Article: The Effects of Fentanyl on Testicular Ischemia-Reperfusion Injury.

Mordeniz, Cengiz / Demirkapu, Mahluga Jafarova / Akgul, Hacı Murat / Karabag, Sevil / Celikkol, Aliye / Yananlı, Hasan Raci

Turkish journal of anaesthesiology and reanimation

2022 Volume 49, Issue 5, Page(s) 373–378

Abstract: Objective: Testicular torsion is a condition that often occurs as a result of the rotation of the spermatic cord in childhood and adolescence in men, manifests with acute pain and causes infertility in the future even if emergency intervention is ... ...

Abstract	Objective: Testicular torsion is a condition that often occurs as a result of the rotation of the spermatic cord in childhood and adolescence in men, manifests with acute pain and causes infertility in the future even if emergency intervention is performed. The aim of this study is to investigate the protective and preventive effects of fentanyl, a potent analgesic agent frequently used in anaesthesia practice, on testicular ischemia reperfusion injury, which manifests through acute pain. Methods: A total of 16 adult male Wistar rats, weighing 200-250 g, were used in this study. They were divided into two groups, consisting of eight animals in each group. Torsion was created in all rats by rotating left testicles 720 clockwise on the day of the experiment. 3 mM of fentanyl was applied intraperitoneally 30 minutes before detorsion to the fentanyl group. Following an hour of ischemia, the left testicle was reinstated, and tissues were repaired according to their physiology. Following 24 hours of reperfusion, the animals were euthanised after taking left testes and blood samples. Results: Fentanyl, administered prior to testicular detorsion, significantly suppressed germ cell damage in torsioned tissue, catalase activity and malondialdehyde levels in blood samples taken from the heart. No significant differences were observed in plasma total thiol concentration, histological score, Leydig cell counts, percentage of necrosis and tubule rupture. Conclusion: These findings show that fentanyl administered before detortion creates a protective effect by preventing testicular ischemia reperfusion injury leading to infertility in the future.
Language	English
Publishing date	2022-01-31
Publishing country	Turkey
Document type	Journal Article
ISSN	2667-677X
ISSN	2667-677X
DOI	10.5152/TJAR.2021.1392
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Article ; Online: Histopathological and molecular findings in 98 cases of endometrial carcinoma: MMR, p53 and next generation sequencing.

Karabağ, Sevil / Şentürk, Mehmetbaki / Söğüt, Fırat Can / Ergül, Özlem Sevinç / Ersoy, Tuğçe

Pathology, research and practice

2022 Volume 241, Page(s) 154275

Abstract: Gynecological malignancies arise from hereditary and somatic mutations, transcriptional aberrations, and genomic alterations influenced by epigenetic factors. This study aims to identify the mutations and their frequency in endometrial carcinomas (EC), ... ...

Abstract	Gynecological malignancies arise from hereditary and somatic mutations, transcriptional aberrations, and genomic alterations influenced by epigenetic factors. This study aims to identify the mutations and their frequency in endometrial carcinomas (EC), and furthermore, to determine the relationship of these mutations with histopathological and immunohistochemical (IHC) parameters. The study was carried out in a retrospective cohort of 98 patients who received treatment upon being diagnosed with EC at a tertiary university hospital in Turkey between 2016 and 2021. The NGS-DNA tumor panel containing 29 genes was used in the study. NGS data of the cases were obtained from state of the evidence Tier 1 and 2 mutations. The relationship of patients' next generation sequencing (NGS) DNA panel results with histopathological parameters and IHC results (MLH1, MSH2, PMS2, MSH6 and p53) were evaluated. In 59 of the 98 cases, mutations were detected in at least one gene investigated with the NGS DNA panel. The most common somatic mutations in endometrial carcinoma were PIK3CA (33.6%), CTNNB1 (16.3%), KRAS (12.2%) and FGFR2 (11.2%) in this cohort. Abnormal p53 was detected by IHC in 15 out of 75 (20%) cases. Loss of expression was observed in at least one mismatch repair (MMR) protein by means of IHC in 39 out of 72 (39.8%) cases. Metastasis was found in 14 out of 82 (14.3%) patients who underwent lymph node dissection. FGFR2 mutation was more common in the group with lymph node metastasis compared to those without metastasis (p = 0.02). We report the mutational landscape of EC in a tertiary referral hospital in northwestern Turkey. Although our data are very limited, we think that the FGFR2 mutation may be associated with lymph node metastasis, but studies with larger patient numbers and longer follow-up periods are needed.
MeSH term(s)	Female ; Humans ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism ; Retrospective Studies ; Lymphatic Metastasis ; High-Throughput Nucleotide Sequencing ; Endometrial Neoplasms/pathology ; DNA Mismatch Repair ; MutL Protein Homolog 1/metabolism ; Microsatellite Instability
Chemical Substances	Tumor Suppressor Protein p53 ; MutL Protein Homolog 1 (EC 3.6.1.3)
Language	English
Publishing date	2022-12-15
Publishing country	Germany
Document type	Journal Article
ZDB-ID	391889-0
ISSN	1618-0631 ; 0344-0338
ISSN (online)	1618-0631
ISSN	0344-0338
DOI	10.1016/j.prp.2022.154275
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Article ; Online: The Results of Pneumatic Balloon Dilatation Treatment in Children with Achalasia: A Single-Center Experience.

Güler, Selen / Aksoy, Betül / Öztan, M Onur / Appak, Yeliz Ç / Kahveci Çelik, Sinem / Onbaşı Karabağ, Şenay / Köylüoğlu, Gökhan / Baran, Maşallah

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology

2023 Volume 34, Issue 9, Page(s) 968–974

Abstract: Background/aims: Achalasia is a primary motility disorder characterized by a relaxation disorder of the lower esophageal sphincter. In pneumatic balloon dilatation, which is one of the treatment methods, the muscle fibers are torn with an endoscopically ...

Abstract	Background/aims: Achalasia is a primary motility disorder characterized by a relaxation disorder of the lower esophageal sphincter. In pneumatic balloon dilatation, which is one of the treatment methods, the muscle fibers are torn with an endoscopically inflated balloon in the lower esophageal sphincter. This study aimed to evaluate the results of long-term pneumatic balloon dilatation treatment in our clinic for children diagnosed with achalasia. Materials and methods: Pediatric patients who underwent pneumatic balloon dilatation with a diagnosis of achalasia in our pediatric gastroenterology clinic between 2016 and 2021 were included in the study. Demographic data of the patients, clinical findings at diagnosis, and follow-up results were evaluated retrospectively. Results: Ten patients who underwent 18 pneumatic balloon dilatation operations were included in the study. The mean follow-up period of the patients was 23.7 ± 14.1 months. It was observed that the procedure was performed once in 3 (30%) patients, twice in 2 (20%) patients, and 3 times in 3 (30%) patients. It is noteworthy that the diameter of the balloon used in the first procedure in patients who needed repeated operations was less than 30 mm. No complications were observed except for chest pain, which was detected in 1 patient. Conclusion: When the patients who needed recurrent dilatation were evaluated, it was noted that the diameter of the balloon in which the first procedure was performed in these patients was smaller. This study is an important contribution to the literature due to the scarcity of the pediatric achalasia data, in which long-term results related to pneumatic balloon dilatation are reported in Turkey.
MeSH term(s)	Humans ; Child ; Esophageal Achalasia/surgery ; Treatment Outcome ; Dilatation/methods ; Retrospective Studies ; Manometry ; Dilatation, Pathologic
Language	English
Publishing date	2023-07-24
Publishing country	Turkey
Document type	Journal Article
ZDB-ID	1340275-4
ISSN	2148-5607 ; 1300-4948
ISSN (online)	2148-5607
ISSN	1300-4948
DOI	10.5152/tjg.2023.22328
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