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Article ; Online: The evaluation of the reverse algorithm for syphilis screening in blood donors

Berkem Rukiye / Karakoç Ayşe Esra

Romanian Journal of Laboratory Medicine, Vol 27, Iss 4, Pp 383-

2019 Volume 388

Abstract: Background: In Turkey, prior to transfusion and apheresis, it is mandatory to screen blood for HBsAg, anti-HCV, anti-HIV 1/2, and syphilis. In recent years, efforts have been made to create effective diagnostic algorithms for screening, and as a ... ...

Abstract	Background: In Turkey, prior to transfusion and apheresis, it is mandatory to screen blood for HBsAg, anti-HCV, anti-HIV 1/2, and syphilis. In recent years, efforts have been made to create effective diagnostic algorithms for screening, and as a screening strategy, many countries have switched from traditional algorithms to reverse algorithms. This study was carried out to evaluate the results we obtained after changing to chemiluminescence immunoassay (CLIA) based reverse algorithm, which is more sensitive and specific than the traditional algorithm and VDRL test we currently use for syphilis screening.
Keywords	blood donors ; syphilis screening tests ; confirmation ; reverse algorithm ; Medicine ; R
Language	English
Publishing date	2019-10-01T00:00:00Z
Publisher	Sciendo
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Pozitif Sinyal Veren Kan Kültürü Şişelerinde EUCAST Doğrudan Hızlı Antimikrobiyal Duyarlılık Testi Yönteminin Değerlendirilmesi.

Erdoğan, Gizem / Karakoç, Ayşe Esra / Yücel, Mihriban / Yağcı, Serap

Mikrobiyoloji bulteni

2021 Volume 55, Issue 4, Page(s) 626–634

Abstract: Early reporting of the antibiotic susceptibility testing (AST) results is essential for the survival of sepsis patients. In 2019, European Committee on Antimicrobial Susceptibility Testing (EUCAST) published a proposal to detect antimicrobial ... ...

Title translation	Evaluation of EUCAST Direct Rapid Antimicrobial Susceptibility Test Method in Blood Culture Bottles with Positive Signal.
Abstract	Early reporting of the antibiotic susceptibility testing (AST) results is essential for the survival of sepsis patients. In 2019, European Committee on Antimicrobial Susceptibility Testing (EUCAST) published a proposal to detect antimicrobial susceptibility from positive blood culture bottles with a rapid antimicrobial susceptibility test (RAST) method in a maximum of eight hours. In this study, it was aimed to evaluate the EUCAST RAST method in blood culture bottles that resulted with positive signal in BacT/ALERT (bioMérieux, France) blood culture system and that showed gram-negative bacteria with single morphology with Gram stain. The study was conducted prospectively between April 2019 and November 2019. Ninety blood culture bottles that we detected single gram negative bacteria morphology by Gram stain were tested according to the EUCAST RAST method, The isolates obtained from the blood culture bottles were studied with the EUCAST disk diffusion method and the Vitek 2 Compact (bioMerieux, France) automated system. The results obtained with RAST were compared with the results of these methods. The turn around time of the RAST method was recorded. Categorical agreement of the RAST method with conventional methods and the very major error rates were determined. Of the 14 isolates not yet covered by the EUCAST HADT method, 12 were determined to be other Enterobacterales members and two as other non-fermentatives. Two isolates were detected with the same morphological characteristics in Gram stain of the blood culture bottle and the same antibiotic susceptibility profile, but with different identification results. These sixteen isolates were excluded from the study. In this study the susceptibility of 74 isolates were determined according to the EUCAST breakpoint tables, of which 31 were Klebsiella pneumoniae, 35 were Escherichia coli, four were Acinetobacter baumannii and four were Pseudomonas aeruginosa. According to the evaluation periods of EUCAST RAST; the susceptibility profile was reported for nine (12%) of E.coli at four hours, eight (11%) at six hours, 18 (24%) at eight hours; three (4%) of K.pneumoniae at four hours, 16 (21%) at six hours, 12 (16%) at eight hours; three of P.aeruginosa (4%) at six hours, one (1%) at eight hours; two of A.baumannii (2%) at six hours and two (2%) at eight hours. The categorical aggrement of the RAST method was 91.8% with the automated system and 96.8% with the disc diffusion method. Very major errors of RAST method compared to the automated system were detected for piperacillin-tazobactam (17.7%), ceftazidime (11.6%) and meropenem (5.6%); and when compared to the disc diffusion method, for cefotaxime (5.7%) and meropenem (6.7%). Our results have shown that EUCAST RAST method can practicaly be performed in routine laboratories to report early results with a low cost. Because of the very major errors it is necessary to confirm the results with the standard methods.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Infective Agents ; Blood Culture ; Gram-Negative Bacteria ; Humans ; Microbial Sensitivity Tests ; Piperacillin, Tazobactam Drug Combination
Chemical Substances	Anti-Bacterial Agents ; Anti-Infective Agents ; Piperacillin, Tazobactam Drug Combination (157044-21-8)
Language	Turkish
Publishing date	2021-10-19
Publishing country	Turkey
Document type	Journal Article
ZDB-ID	985146-x
ISSN	0374-9096
ISSN	0374-9096
DOI	10.5578/mb.20219713
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Article: Sepsis Ön Tanılı Hastalarda Tam Kandan ve Kan Kültür Şişesinden Multipleks Gerçek Zamanlı Polimeraz Zincir Reaksiyonu ile Hızlı Etken Tayininin Konvansiyonel Yöntemlerle Karşılaştırılması.

Erdoğan, Gizem / Karakoç, Ayşe Esra / Yağcı, Serap / Alp, Güray / Kaymak, Çetin

Mikrobiyoloji bulteni

2022 Volume 56, Issue 3, Page(s) 432–448

Abstract: In this study, it was aimed to evaluate the use of multiplex real time polymerase chain reaction (RtPCR) method, directly from whole blood and blood culture bottles of intensive care unit patients with a pre-diagnosis of sepsis for the determination of ... ...

Title translation	Comparison of the Multiplex Real Time Polymerase Chain Reaction Method Directly from Whole Blood and from Blood Culture Bottle with the Conventional Method for the Etiological Diagnosis in Patients with Pre-Diagnosis of Sepsis.
Abstract	In this study, it was aimed to evaluate the use of multiplex real time polymerase chain reaction (RtPCR) method, directly from whole blood and blood culture bottles of intensive care unit patients with a pre-diagnosis of sepsis for the determination of the causative agent by comparing it with the conventional method. The study was carried out prospectively between November 2019 and August 2020. Ninety patients hospitalized with a pre-diagnosis of sepsis were included in the study. Samples were collected simultaneously with aseptic technique as whole blood and at least two sets of blood cultures. Blood culture bottles were monitored by the BACT/ALERT 3D (bioMerieux, France) instrument. Blood culture bottles were studied both by Sepsis Pathogens Panel Kit v1 (Anatolia, Turkey) using the multiplex Rt-PCR and with the conventional culture methods. The duration of detection and reporting of the multiplex Rt-PCR methods were compared with the conventional method. In this study, a total of 90 whole blood samples and 280 blood culture bottles were collected from the patients. It was found that 73 (81%) patients had already been administered antibiotic treatment at the time of blood sampling. Conventional blood culture was accepted as the gold standard in the diagnosis of sepsis. Rt-PCR performed from blood culture bottles was found to have a sensitivity of 89.58%; specificity of 57.14%; positive predictive value of 70.49%; negative predictive value of 82.76% and accuracy of 74.44% (p= 0.019). On the basis of the bacterial species, the sensitivity of the Rt-PCR method was determined to be between 66.7-100% and the specificity was between 86.6-100%. The Rt-PCR performed from whole blood, was found to have a sensitivity of 11.67% and a specificity of 96.67%; positive predictive value of 87.50%; negative predictive value of 35.37% and accuracy of 40% (p= 1.684). In this study, the duration of reporting of blood culture results was in average 116:50 hours, that of PCR from blood culture bottles was 80:57 hours, and that of PCR from whole blood samples was 15:38 hours (p<0.001). It was determined that the PCR from whole blood shortened the reporting time by an average of 101:12 hours (approximately four days), and that the PCR from blood culture bottles shortened the reporting time by an average of 35:53 hours (1.5 days) compared to the conventional method. In this study, the results determined by the Sepsis Pathogens Panel Kit v1 (Anatolia, Turkey) performed from blood culture bottles were superior compared to the conventional method. It has been determined that improvement is required for the usage of the sepsis kit directly from whole blood. On the other hand, considering the species detected by the blood culture method and not by the assay, the species range detected by the multiplex assay panel needs to be improved.
MeSH term(s)	Bacteria ; Blood Culture ; Humans ; Multiplex Polymerase Chain Reaction ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Sepsis/diagnosis ; Sepsis/microbiology
Language	Turkish
Publishing date	2022-08-04
Publishing country	Turkey
Document type	Journal Article
ZDB-ID	985146-x
ISSN	0374-9096
ISSN	0374-9096
DOI	10.5578/mb.20229705
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Article ; Online: The comparison of COVID-19 vs seasonal influenza in children.

Yayla, Burcu Ceylan Cura / Aykac, Kubra / Boluk, Oguz / Fidanci, Ilknur / Tasar, Medine Aysin / Pamuk, Utku / Karakoc, Ayse Esra / Karakaya, Jale / Ozsurekci, Yasemin

Pediatrics international : official journal of the Japan Pediatric Society

2023 Volume 65, Issue 1, Page(s) e15684

Abstract: Background: Influenza in children has been well described, whereas there has been a paucity of pediatric data regarding COVID-19. It is crucial for clinicians to differentiate cases of COVID-19 from cases of influenza because of the upcoming influenza ... ...

Abstract	Background: Influenza in children has been well described, whereas there has been a paucity of pediatric data regarding COVID-19. It is crucial for clinicians to differentiate cases of COVID-19 from cases of influenza because of the upcoming influenza season in the new pandemic era. Methods: This retrospective study included pediatric patients who were diagnosed with laboratory-confirmed COVID-19 between March and September 2020, or seasonal influenza between October 2019 and March 2020. Results: A total of 315 children were included in this study; 151 were diagnosed with influenza and 164 had confirmed COVID-19. The median age of patients with COVID-19 was 10 years (interquartile range [IQR]: 3-15 years), whereas the median age of patients with influenza was 4 years (IQR: 1-6 years) (p = 0.001). In the COVID-19 group, 6.3% of patients had underlying diseases, the most frequent being neurological conditions (3%). In the influenza group, 20.9% of patients had an underlying disease, the most frequent being asthma (14.5%). Fever (odds ratio [OR]: 20.476; 95% confidence interval [CI]: 2.438-171.995; p = 0.005), dyspnea/tachypnea (OR 13.950; 95% CI: 2.607-74.634; p = 0.002), and increased C-reactive protein (CRP) (OR: 7.650; 95% CI: 2.094-27.955; p = 0.002) were main predictors of influenza diagnosis in comparison to COVID-19. Lymphopenia was detected in 43.2% of patients with influenza and 19.9% of patients with COVID-19 (p = 0.001). Conclusions: The accurate differentiation between "influenza or COVID-19" seems possible by evaluating a combination of factors including cough, fever, vomiting, leucopenia, lymphopenia, pneumonia, in pediatric patients with high CRP as well as age.
MeSH term(s)	Child ; Humans ; Child, Preschool ; Adolescent ; Infant ; COVID-19/diagnosis ; COVID-19/epidemiology ; Influenza, Human/diagnosis ; Influenza, Human/epidemiology ; Seasons ; Retrospective Studies ; SARS-CoV-2 ; Lymphopenia/epidemiology
Language	English
Publishing date	2023-12-01
Publishing country	Australia
Document type	Journal Article
ZDB-ID	1470376-2
ISSN	1442-200X ; 1328-8067
ISSN (online)	1442-200X
ISSN	1328-8067
DOI	10.1111/ped.15684
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Article: Anti-dense fine speckled 70/lens epithelium derived growth factor p75 otoantikorunun otoimmün hastalıklarda klinik tanıya katkısının araştırılması.

Türkoğlu, Gamze / Berkem, Rukiye / Karakoç, Ayşe Esra

Mikrobiyoloji bulteni

2018 Volume 52, Issue 4, Page(s) 413–424

Abstract: Antinuclear antibodies (ANA) are the autoantibodies that are produced against nuclear antigens in the cell nucleus and/or cytoplasm, and are one of the important diagnostic criteria in systemic autoimmune rheumatic diseases (SARD). Until today, several ... ...

Title translation	Investigation of the diagnostic value of anti-dense fine speckled 70/lens epithelium derived growth factor p75 autoantibody for autoimmune diseases.
Abstract	Antinuclear antibodies (ANA) are the autoantibodies that are produced against nuclear antigens in the cell nucleus and/or cytoplasm, and are one of the important diagnostic criteria in systemic autoimmune rheumatic diseases (SARD). Until today, several methods have been developed for detecting ANA's. However, indirect immunofluorescence (IIF) technique, that is also known as one of the oldest methods, is still the most commonly used one. Typically, anti-dense fine speckled 70/Lens epithelium derived growth factor p75 (anti-DFS70/LEDGF p75) autoantibody can be detected via IIF method where in HEp-2 (human larynx carcinoma) cells are used. The dense fine speckled (DFS) pattern method can be masked and remain unnoticed by the IIF method when it exists with the other ANA. Anti-DFS70 autoantibodies seldomly appear in SARD patients compared to healthy individuals. Moreover, these antibodies may appear in different chronic inflammatory conditions like interstitial cystitis, chronic fatigue syndrome, atopic dermatitis and Vogt-Koyanagi-Harada syndrome. The aim of this study was to determine the frequency of anti-DFS70 autoantibodies by immunblot (IB) method in patients sera with and without DFS70 staining pattern by IIF and to determine if the presence of anti-DFS70 has a clinical impact when included in ANA testing algorithm. In our study, a total of 60 patients' sera in which DFS pattern was defined by IIF method and 67 patients' sera in which other patterns observed were included in the study and anti-DFS70 autoantibody was investigated by IB method in these sera. In 67 patient samples which have shown the other patterns three (4.5%) samples were determined to be anti-DFS70 positive by IB. In 55 patients who were determined to have IIF-DFS pattern (+)/IB anti-DFS70 (+), 6 (11%) were diagnosed as SARD and the other antinuclear antibodies (ANA) were found as negative by IB. In the other group with the other ANA patterns detected, none of the SARD-diagnosed 22 patients had shown anti-DFS70 by IB method. Sixteen (26.6%) samples in the group that was positive for the IIF-DFS pattern were obtained from rheumatology and physical therapy and rehabilitation clinics, 32 (47.7%) samples were from the group in which other patterns observed and were also obtained from those clinics. DFS pattern was detected significantly more frequent in the samples from other clinics in comparison to the samples from rheumatology and physical therapy and rehabilitation clinics (p= 0.018). In our study, it was concluded that DFS pattern can be defined by IIF method by only specialists, however, since homogeneous-like and mixed patterns can be confused especially in low titers, there is a need for a second well-validated immunological test that could detect anti-DFS70 auto-antibody.
MeSH term(s)	Adaptor Proteins, Signal Transducing/immunology ; Antibodies, Antinuclear/blood ; Autoantibodies/blood ; Autoantibodies/metabolism ; Autoimmune Diseases/blood ; Autoimmune Diseases/diagnosis ; Fluorescent Antibody Technique, Indirect ; Humans ; Intercellular Signaling Peptides and Proteins/immunology ; Transcription Factors/immunology
Chemical Substances	Adaptor Proteins, Signal Transducing ; Antibodies, Antinuclear ; Autoantibodies ; Intercellular Signaling Peptides and Proteins ; PSIP1 protein, human ; Transcription Factors ; lens epithelium-derived growth factor
Language	Turkish
Publishing date	2018-12-06
Publishing country	Turkey
Document type	Journal Article
ZDB-ID	985146-x
ISSN	0374-9096
ISSN	0374-9096
DOI	10.5578/mb.67385
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Article ; Online: Pneumococcal carriage in children with COVID-19.

Aykac, Kubra / Ozsurekci, Yasemin / Cura Yayla, Burcu Ceylan / Evren, Kubra / Lacinel Gurlevik, Sibel / Oygar, Pembe Derin / Yucel, Mihriban / Karakoc, Ayse Esra / Alp, Alpaslan / Cengiz, Ali Bulent / Ceyhan, Mehmet

Human vaccines & immunotherapeutics

2021 Volume 17, Issue 6, Page(s) 1628–1634

Abstract: ... ...

Abstract	Background
MeSH term(s)	COVID-19/complications ; COVID-19/microbiology ; Carrier State/epidemiology ; Carrier State/microbiology ; Child ; Humans ; Nasopharynx/microbiology ; Pandemics ; Pneumococcal Infections/epidemiology ; Retrospective Studies ; Streptococcus pneumoniae ; Turkey
Language	English
Publishing date	2021-01-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2664176-8
ISSN	2164-554X ; 2164-5515
ISSN (online)	2164-554X
ISSN	2164-5515
DOI	10.1080/21645515.2020.1849516
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Article: Detection of hepatitis B virus deoxyribonucleic acid using real-time polymerase chain reaction method in pooled-plasma samples of Turkish blood donors.

Karakoc, Ayse Esra / Berkem, Rukiye / Beyaz, Elif

Clinical laboratory

2009 Volume 55, Issue 5-6, Page(s) 229–234

Abstract: Background: The extent and the number of routine tests performed for blood donor screening to avoid infections transmitted through blood transfusion have been gradually increasing worldwide and is determined nationally. Following the publication of the ... ...

Abstract	Background: The extent and the number of routine tests performed for blood donor screening to avoid infections transmitted through blood transfusion have been gradually increasing worldwide and is determined nationally. Following the publication of the new "blood and blood components regulation", the blood banking and transfusion system is through a reorganization stage in Turkey. The national guideline including the donor screening algorithms is to be published which requires national data to be created. The aim of this study is to investigate HBV DNA in pooled plasma samples of blood donors in Turkey, which is a middle endemicity country for HBV infection. Study design and methods: Presence of HBV DNA was investigated using real-time polymerase chain reaction (RT-PCR) method in 187 pooled plasma samples prepared from 4484 blood donors, whose screening tests were found to be negative. The seropositivity for HBsAg of the blood donors in the study blood center is 2.2%. Results: The rate of false-positivity of the RT-PCR method was found to be 1.6% for the mini pools and 0.04% for the individual blood donors. HBV DNA was not detected in any of the donor bloods. Conclusion: The hospital blood centers in Turkey still have a high proportion of first time donors and replacement donors; also seropositivity of HBsAg in Turkish blood donors is high compared to Europe and US. Our data pointed to the high false positivity rate of RT PCR method which needs to be evaluated in creating national donor screening algorithms.
MeSH term(s)	Adolescent ; Adult ; Aged ; Algorithms ; Blood Donors ; Blood Transfusion/methods ; Blood Transfusion/standards ; DNA, Viral/blood ; DNA, Viral/genetics ; False Positive Reactions ; Female ; Hepatitis B/blood ; Hepatitis B/diagnosis ; Hepatitis B/prevention & control ; Hepatitis B/transmission ; Hepatitis B virus/genetics ; Hepatitis B virus/isolation & purification ; Humans ; Male ; Middle Aged ; Polymerase Chain Reaction/methods ; Turkey ; Young Adult
Chemical Substances	DNA, Viral
Language	English
Publishing date	2009
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1307629-2
ISSN	1433-6510 ; 0941-2131
ISSN	1433-6510 ; 0941-2131
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Article ; Online: Evaluation of Invasive and Noninvasive Methods for the Diagnosis of Helicobacter Pylori Infection

Cosgun, Yasemin / Yildirim, Abdullah / Yucel, Mihriban / Karakoc, Ayse Esra / Koca, Gokhan / Gonultas, Alpaslan / Gursoy, Gul / Ustun, Huseyin / Korkmaz, Meliha

Asian Pacific journal of cancer prevention : APJCP

2016 Volume 17, Issue 12, Page(s) 5265–5272

Language	English
Publishing date	2016--01
Publishing country	Thailand
Document type	Journal Article
ISSN	2476-762X
ISSN (online)	2476-762X
DOI	10.22034/APJCP.2016.17.12.5265
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Article ; Online: Ureaplasma urealyticum: Presence among Sexually Transmitted Diseases.

Esen, Berrin / Gozalan, Aysegul / Sevindi, Demet Furkan / Demirbas, Arif / Onde, Ufuk / Erkayran, Ugurkan / Karakoc, Ayse Esra / Hasçiçek, Ahmet Metin / Ergün, Yusuf / Adiloglu, Ali Kudret

Japanese journal of infectious diseases

2017 Volume 70, Issue 1, Page(s) 75–79

Abstract: The aim of this study was to detect the presence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Trichomonas vaginalis, and Ureaplasma urealyticum in genital specimens of symptomatic patients. This study also ... ...

Abstract	The aim of this study was to detect the presence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Trichomonas vaginalis, and Ureaplasma urealyticum in genital specimens of symptomatic patients. This study also examined the role of U. urealyticum in infections of the lower genital tract. Cervical and urethral samples from 96 patients (46 males, 50 females) were tested using the Seeplex(
MeSH term(s)	Adolescent ; Adult ; Chlamydia trachomatis/isolation & purification ; Coinfection/epidemiology ; Coinfection/microbiology ; Coinfection/parasitology ; Female ; Genitalia/microbiology ; Genitalia/parasitology ; Humans ; Male ; Middle Aged ; Mycoplasma/isolation & purification ; Neisseria gonorrhoeae/isolation & purification ; Sexually Transmitted Diseases/epidemiology ; Sexually Transmitted Diseases/microbiology ; Sexually Transmitted Diseases/parasitology ; Surveys and Questionnaires ; Trichomonas vaginalis/isolation & purification ; Ureaplasma Infections/epidemiology ; Ureaplasma Infections/microbiology ; Ureaplasma urealyticum/isolation & purification ; Young Adult
Language	English
Publishing date	2017-01-24
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1478383-6
ISSN	1884-2836 ; 1344-6304
ISSN (online)	1884-2836
ISSN	1344-6304
DOI	10.7883/yoken.JJID.2015.258
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Article: Investigation of an algorithm for anti HCV EIA reactivity in blood donor screening in Turkey in the absence of nucleic acid amplification screening.

Karakoc, Ayse Esra / Berkem, Rukiye / Irmak, Hasan / Demiroz, Ali Pekcan / Yenicesu, Idil / Ertugrul, Nigar / Arslan, Önder / Kemahli, Sabri / Yilmaz, Sevinc / Ozcebe, Osman / Kara, Abdurrahman / Ozet, Gulsum / Acikgoz, Ziya Cibali / Acikgoz, Tulin

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis

2017 Volume 56, Issue 5, Page(s) 732–737

Abstract: Purpose: In this study we aimed to propose an algorithm for initial anti HCV EIA reactive blood donations in Turkey where nucleic acid amplification tests are not yet obligatory for donor screening.: Methods: A total of 416 anti HCV screening test ... ...

Abstract	Purpose: In this study we aimed to propose an algorithm for initial anti HCV EIA reactive blood donations in Turkey where nucleic acid amplification tests are not yet obligatory for donor screening. Methods: A total of 416 anti HCV screening test reactive donor samples collected from 13 blood centers from three cities in Turkey were tested in duplicate by Ortho HCV Ab Version 3.0 and Radim HCV Ab. All the repeat reactive samples were tested by INNO-LIA HCV Ab 3.0 or Chiron RIBA HCV 3.0 and Abbott Real Time HCV. Intra-assay correlations were calculated with Pearson r test. ROC analysis was used to study the relationship between EIA tests and the confirmatory tests. Results: The number of repeat reactive results with Ortho EIA were 221 (53.1%) whereas that of microEIA, 62 (14.9%). Confirmed positivity rate was 14.6% (33/226) by RIBA and 10.6% (24/226) by NAT. Reactive PCR results were predicted with 100% sensitivity and 95% specificity with S/CO levels of 8.1 with Ortho EIA and 3.4 with microEIA. Conclusions: Repeat reactivity rates declined with a second HCV antibody assay. Samples repeat reactive with one HCV antibody test and negative with the other were all NAT negative. All the NAT reactive samples were RIBA positive. None of the RIBA indeterminate or negative samples were NAT reactive. Considering the threshold values for EIA kits determined by ROC analysis NAT was decided to be performed for the samples above the threshold value and a validated supplemental HCV antibody test for the samples below.
MeSH term(s)	Blood Donors ; Donor Selection/methods ; Hepatitis C/blood ; Humans ; Nucleic Acid Amplification Techniques/methods ; Turkey
Language	English
Publishing date	2017-10
Publishing country	England
Document type	Journal Article
ZDB-ID	2046795-3
ISSN	1878-1683 ; 1473-0502
ISSN (online)	1878-1683
ISSN	1473-0502
DOI	10.1016/j.transci.2017.08.025
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Inter-library loan at ZB MED

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In stock of ZB MED Cologne/Königswinter

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In stock of ZB MED Cologne/Königswinter

Order via subito