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  1. Article ; Online: Mimicking natural electrical environment with cellulose acetate scaffolds enhances collagen formation of osteoblasts.

    Szewczyk, Piotr K / Berniak, Krzysztof / Knapczyk-Korczak, Joanna / Karbowniczek, Joanna E / Marzec, Mateusz M / Bernasik, Andrzej / Stachewicz, Urszula

    Nanoscale

    2023  Volume 15, Issue 15, Page(s) 6890–6900

    Abstract: The medical field is continuously seeking new solutions and materials, where cellulose materials due to their high biocompatibility have great potential. Here we investigate the applicability of cellulose acetate (CA) electrospun fibers for bone tissue ... ...

    Abstract The medical field is continuously seeking new solutions and materials, where cellulose materials due to their high biocompatibility have great potential. Here we investigate the applicability of cellulose acetate (CA) electrospun fibers for bone tissue regeneration. For the first time we show the piezoelectric properties of electrospun CA fibers
    MeSH term(s) Tissue Scaffolds/chemistry ; Tissue Engineering ; Osteoblasts ; Cellulose/pharmacology ; Cellulose/chemistry ; Collagen/chemistry
    Chemical Substances acetylcellulose (3J2P07GVB6) ; Cellulose (9004-34-6) ; Collagen (9007-34-5)
    Language English
    Publishing date 2023-04-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2515664-0
    ISSN 2040-3372 ; 2040-3364
    ISSN (online) 2040-3372
    ISSN 2040-3364
    DOI 10.1039/d3nr00014a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Enhanced Cells Anchoring to Electrospun Hybrid Scaffolds With PHBV and HA Particles for Bone Tissue Regeneration.

    Karbowniczek, Joanna E / Kaniuk, Łukasz / Berniak, Krzysztof / Gruszczyński, Adam / Stachewicz, Urszula

    Frontiers in bioengineering and biotechnology

    2021  Volume 9, Page(s) 632029

    Abstract: Hybrid materials combining organic and inorganic compounds used as scaffolds are highly beneficial in bone regeneration. In this study, we successfully produced by blend electrospinning poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) scaffolds ... ...

    Abstract Hybrid materials combining organic and inorganic compounds used as scaffolds are highly beneficial in bone regeneration. In this study, we successfully produced by blend electrospinning poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) scaffolds enriched with hydroxyapatite (HA) particles to biomimic bone tissue for improved and faster regeneration processes. The morphology, fiber diameters, and composition of the scaffolds were investigated by scanning electron microscopy (SEM) techniques followed by focused ion beam (FIB) sectioning to verify HA particles integration with PHBV fibers.
    Language English
    Publishing date 2021-02-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2021.632029
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  3. Article: Cell Integration with Electrospun PMMA Nanofibers, Microfibers, Ribbons, and Films: A Microscopy Study.

    Ura, Daniel P / Karbowniczek, Joanna E / Szewczyk, Piotr K / Metwally, Sara / Kopyściański, Mateusz / Stachewicz, Urszula

    Bioengineering (Basel, Switzerland)

    2019  Volume 6, Issue 2

    Abstract: Tissue engineering requires properly selected geometry and surface properties of the scaffold, to promote in vitro tissue growth. In this study, we obtained three types of electrospun poly(methyl methacrylate) (PMMA) scaffolds-nanofibers, microfibers, ... ...

    Abstract Tissue engineering requires properly selected geometry and surface properties of the scaffold, to promote in vitro tissue growth. In this study, we obtained three types of electrospun poly(methyl methacrylate) (PMMA) scaffolds-nanofibers, microfibers, and ribbons, as well as spin-coated films. Their morphology was imaged by scanning electron microscopy (SEM) and characterized by average surface roughness and water contact angle. PMMA films had a smooth surface with roughness,
    Language English
    Publishing date 2019-05-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2746191-9
    ISSN 2306-5354
    ISSN 2306-5354
    DOI 10.3390/bioengineering6020041
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  4. Article ; Online: Enhanced osteoblasts adhesion and collagen formation on biomimetic polyvinylidene fluoride (PVDF) films for bone regeneration.

    Szewczyk, Piotr K / Metwally, Sara / Krysiak, Zuzanna J / Kaniuk, Łukasz / Karbowniczek, Joanna E / Stachewicz, Urszula

    Biomedical materials (Bristol, England)

    2019  Volume 14, Issue 6, Page(s) 65006

    Abstract: Bone tissue engineering can be utilized to study the early events of osteoconduction. Fundamental research in cell adhesion to various geometries and proliferation has shown the potential of extending it to implantable devices for regenerative medicine. ... ...

    Abstract Bone tissue engineering can be utilized to study the early events of osteoconduction. Fundamental research in cell adhesion to various geometries and proliferation has shown the potential of extending it to implantable devices for regenerative medicine. Following this concept in our studies, first, we developed well-controlled processing of polyvinylidene fluoride (PVDF) film to obtain a surface biomimicking ECM. We optimized the manufacturing dependent on humidity and temperature during spin-coating of a polymer solution. The mixture of solvents such as dimethylacetamide and acetone together with high humidity conditions led to a biomimetic, highly porous and rough surface, while with lower humidity and high temperatures drying allowed us to obtain a smooth and flat PVDF film. The roughness of the PVDF film was biofabricated and compared to smooth films in cell culture studies for adhesion and proliferation of osteoblasts. The bioinspired roughness of our films enhanced the osteoblast adhesion by over 44%, and there was collagen formation already after 7 days of cell culturing that was proved via scanning electron microscopy observation, light microscopy imaging after Sirius Red staining, and proliferation test such as MTS. Cell development, via extended filopodia, formed profoundly on the rough PVDF surface, demonstrated the potential of the structural design of biomimetic surfaces to enhance further bone tissue regeneration.
    MeSH term(s) Biomimetic Materials ; Bone Regeneration ; Bone and Bones ; Cell Adhesion ; Cell Line, Tumor ; Cell Proliferation ; Collagen/chemistry ; Humans ; Humidity ; Microscopy, Electron, Scanning ; Osteoblasts/cytology ; Osteoblasts/drug effects ; Polymers/chemistry ; Polyvinyls/chemistry ; Regenerative Medicine/methods ; Surface Properties ; Tissue Engineering/methods
    Chemical Substances Polymers ; Polyvinyls ; polyvinylidene fluoride (24937-79-9) ; Collagen (9007-34-5)
    Language English
    Publishing date 2019-09-20
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2265222-X
    ISSN 1748-605X ; 1748-6041
    ISSN (online) 1748-605X
    ISSN 1748-6041
    DOI 10.1088/1748-605X/ab3c20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Thermal insulation design bioinspired by microstructure study of penguin feather and polar bear hair.

    Metwally, Sara / Martínez Comesaña, Sara / Zarzyka, Mateusz / Szewczyk, Piotr K / Karbowniczek, Joanna E / Stachewicz, Urszula

    Acta biomaterialia

    2019  Volume 91, Page(s) 270–283

    Abstract: Nature is an amazing source of inspiration for the design of thermal insulation strategies, which are key for saving energy. In nature, thermal insulation structures, such as penguin feather and polar bear hair, are well developed; enabling the animals' ... ...

    Abstract Nature is an amazing source of inspiration for the design of thermal insulation strategies, which are key for saving energy. In nature, thermal insulation structures, such as penguin feather and polar bear hair, are well developed; enabling the animals' survival in frigid waters. The detailed microscopy investigations conducted in this study, allowed us to perform microstructural analysis of these thermally insulating materials, including statistical measurements of keratin fiber and pore dimensions directly from high resolution Scanning Electron Microscope (SEM) images. The microscopy study revealed many similarities in both materials, and showed the importance of their hierarchically-organized porous structure. Finally, we propose the schematic configuration of a thermally-insulating structure, based on the penguin feather and polar bear hair. These optimized thermal-insulator systems indicate the road maps for future development, and new approaches in the design of material properties. STATEMENT OF SIGNIFICANCE: We present the first detailed comparison of microstructures of penguin feather and polar bear hair for designing optimum thermal insulation properties. This unique study involves the measurement of the sizes of pores and fibers of these two keratin-based materials, including the investigation of their 3D arrangements. We revealed porosity interconnection, especially in polar bear hair, which is one of the key designs exhibited by thermal insulation materials.
    MeSH term(s) Animals ; Biomimetic Materials/chemistry ; Feathers ; Hair ; Spheniscidae ; Ursidae
    Language English
    Publishing date 2019-04-18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2173841-5
    ISSN 1878-7568 ; 1742-7061
    ISSN (online) 1878-7568
    ISSN 1742-7061
    DOI 10.1016/j.actbio.2019.04.031
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Surface-Potential-Controlled Cell Proliferation and Collagen Mineralization on Electrospun Polyvinylidene Fluoride (PVDF) Fiber Scaffolds for Bone Regeneration.

    Szewczyk, Piotr K / Metwally, Sara / Karbowniczek, Joanna E / Marzec, Mateusz M / Stodolak-Zych, Ewa / Gruszczyński, Adam / Bernasik, Andrzej / Stachewicz, Urszula

    ACS biomaterials science & engineering

    2019  Volume 5, Issue 2, Page(s) 582–593

    Abstract: This study represents the unique analysis of the electrospun scaffolds with the controlled and stable surface potential without any additional biochemical modifications for bone tissue regeneration. We controlled surface potential of polyvinylidene ... ...

    Abstract This study represents the unique analysis of the electrospun scaffolds with the controlled and stable surface potential without any additional biochemical modifications for bone tissue regeneration. We controlled surface potential of polyvinylidene fluoride (PVDF) fibers with applied positive and negative voltage polarities during electrospinning, to obtain two types of scaffolds PVDF(+) and, PVDF(-). The cells' attachments to PVDF scaffolds were imaged in great details with advanced scanning electron microscopy (SEM) and 3D tomography based on focus ion beam (FIB-SEM). We presented the distinct variations in cells shapes and in filopodia and lamellipodia formation according to the surface potential of PVDF fibers that was verified with Kelvin probe force microscopy (KPFM). Notable, cells usually reach their maximum spread area through increased proliferation, suggesting the stronger adhesion, which was indeed double for PVDF(-) scaffolds having surface potential of -95 mV. Moreover, by tuning the surface potential of PVDF fibers, we were able to enhance collagen mineralization for possible use in bone regeneration. The scaffolds built of PVDF(-) fibers demonstrated the greater potential for bone regeneration than PVDF(+), showing after 7 days in osteoblasts culture produce well-mineralized osteoid required for bone nodules. The collagen mineralization was confirmed with energy dispersive X-ray spectroscopy (EDX) and Sirius Red staining, additionally the cells proliferation with fluorescence microscopy and Alamar Blue assays. The scaffolds made of PVDF fibers with the similar surface potential to the cell membranes promoting bone growth for next-generation tissue scaffolds, which are on a high demand in bone regenerative medicine.
    Language English
    Publishing date 2019-01-03
    Publishing country United States
    Document type Journal Article
    ISSN 2373-9878
    ISSN (online) 2373-9878
    DOI 10.1021/acsbiomaterials.8b01108
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