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  1. Article ; Online: The Use of Nanobiotechnology in Immunology and Vaccination

    Reza Keikha / Karim Daliri / Ali Jebali

    Vaccines, Vol 9, Iss 2, p

    2021  Volume 74

    Abstract: Nanotechnology uses the unique properties of nanostructures with a size of 1 to 200 nanometers. Different nanoparticles have shown great promise for the production of new vaccines and drugs. Nanostructures can be used to deliver immunological compounds ... ...

    Abstract Nanotechnology uses the unique properties of nanostructures with a size of 1 to 200 nanometers. Different nanoparticles have shown great promise for the production of new vaccines and drugs. Nanostructures can be used to deliver immunological compounds more effectively than microstructures to target sites. Different nanostructures can be applied to form a new generation of vaccines, adjuvants, and immune system drugs. The goal of nanotechnology is to better respond to a wide range of infectious and non-infectious diseases.
    Keywords nanotechnology ; nanostructures ; immunology ; vaccination ; Medicine ; R
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Silica nanowire conjugated with loop-shaped oligonucleotides: A new structure to silence cysteine proteinase gene in Leishmania tropica

    Bafghi, Ali Fatahi / Ali Jebali / Karim Daliri

    Colloids and Surfaces B: Biointerfaces. 2015 Dec. 01, v. 136

    2015  

    Abstract: The main aim of this study was to evaluate the capability of silica nanowire conjugated with loop-shaped oligonucleotides (SNWCLSOs) to silence cysteine proteinase b (Cpb) gene in Leishmania (L) tropica. On the other hand, its toxicity on amastigotes and ...

    Abstract The main aim of this study was to evaluate the capability of silica nanowire conjugated with loop-shaped oligonucleotides (SNWCLSOs) to silence cysteine proteinase b (Cpb) gene in Leishmania (L) tropica. On the other hand, its toxicity on amastigotes and mouse peritoneal macrophages was evaluated by 5-diphenyl-tetrazolium bromide (MTT) assay. For control, two loop-shaped oligonucleotides (LSO) were considered. LSO1 and LSO2 were 5ʹ-NH2-cccccaaaaaaaaaaaaaaaaaaaaaaaaaggggg-COOH-3ʹ and LSO2: 5ʹ-NH2-cccccttttttttttttttttttttttttttttttttttttttggggg-COOH-3ʹ, respectively. After 72h incubation at 37°C, AMSNW, LSO1, and LSO2 had no remarkable toxicity on L. tropica amastigote (2×105/mL) and mouse peritoneal macrophages (2×105/mL). In case of SNWCLSOs, they had high toxicity on L. tropica amastigote, but they had no effect on mouse peritoneal macrophages. At concentrations of 1, 10, and 25μg/mL, AMSNW, LSO1 and LSO2 had no effect on the gene expression. But, at concentration of 50 and 100μg/mL, decrease of gene expression was observed. In case of SNWCLSOs, they could dramatically decrease the gene expression. It could be concluded that since SNWCLSOs could silence Cpb gene with no remarkable toxicity, they are good choice for treat cutaneous leishmaniasis in future. As a new agent, it must be checked in vivo.
    Keywords amastigotes ; colloids ; cysteine proteinases ; gene expression ; genes ; Leishmania tropica ; macrophages ; mice ; nanowires ; oligonucleotides ; silica ; toxicity
    Language English
    Dates of publication 2015-1201
    Size p. 323-328.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1500523-9
    ISSN 1873-4367 ; 0927-7765
    ISSN (online) 1873-4367
    ISSN 0927-7765
    DOI 10.1016/j.colsurfb.2015.09.028
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Magnesium oxide nanoparticles coated with glucose can silence important genes of Leishmania major at sub-toxic concentrations

    Bafghi, Ali Fatahi / Ali Jebali / Karim Daliri / Mojtaba Daghighi

    Colloids and Surfaces B: Biointerfaces. 2015 Dec. 01, v. 136

    2015  

    Abstract: The aim of this study was to investigate the effect of magnesium oxide nanoparticles (MgO NPs) and MgO NPs coated with glucose (MONPCG) on Leishmania (L) major. First, the promastigotes of L. major were separately incubated with serial concentrations of ... ...

    Abstract The aim of this study was to investigate the effect of magnesium oxide nanoparticles (MgO NPs) and MgO NPs coated with glucose (MONPCG) on Leishmania (L) major. First, the promastigotes of L. major were separately incubated with serial concentrations of MgO NPs and MONPCG for 24, 48, and 72h at 37°C. Then, the cell viability of promastigotes was evaluated by MTT assay. On the other hand, the relative expression of Cpb and GP63 genes was detected by quantitative-real time PCR. Based on results, the increase of concentration, both MgO NPs and MONPCG, and incubation time led to decrease of cell viability. Moreover, the expression of Cpb and GP63 genes was decreased with increase of concentration of MgO NPs and MONPCG. Also, the increase of incubation time led to decrease of their expression in MgO NPs treated promastogotes. But, in case of MONPCG treated promastogotes, the increase of incubation time did not change the expression of Cpb and GP63. Interestingly, MONPCG could silence Cpb and GP63 genes better than MgO NPs. Note, the capability was also seen at sub-toxic concentrations of MONPCG.
    Keywords cell viability ; colloids ; genes ; glucose ; Leishmania major ; magnesium oxide ; nanoparticles ; polymerase chain reaction ; promastigotes
    Language English
    Dates of publication 2015-1201
    Size p. 300-304.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1500523-9
    ISSN 1873-4367 ; 0927-7765
    ISSN (online) 1873-4367
    ISSN 0927-7765
    DOI 10.1016/j.colsurfb.2015.09.029
    Database NAL-Catalogue (AGRICOLA)

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    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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