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  1. Article ; Online: Generation of specific antibody against recombinant major coat protein of Beet necrotic yellow vein virus and its application for detection of rhizomania disease

    Karimzade, Marziye / Safarnejad, Mohammad Reza / Aminzadeh, Saeed / Kazemzadeh-Beneh, Hashem / Hemmati, Farshad / Shams-Bakhsh, Masoud

    Archives of Phytopathology and Plant Protection. 2023 Jan. 2, v. 56, no. 1 p.10-28

    2023  

    Abstract: Beet necrotic yellow vein virus (BNYVV) is one of the main contributors to economic losses in sugar beet production. The present study generated a polyclonal antibody that detects BNYVV. The conserved genomic region of all BNYVV isolates gene encoding ... ...

    Abstract Beet necrotic yellow vein virus (BNYVV) is one of the main contributors to economic losses in sugar beet production. The present study generated a polyclonal antibody that detects BNYVV. The conserved genomic region of all BNYVV isolates gene encoding the viral major coat protein (BNYVV-CP) was amplified, cloned, sequenced, and expressed in E. coli strain BL21 (DE3). The expressed protein was purified under native conditions by affinity chromatography. The purified BNYVV CP was used as a recombinant antigen to raise polyclonal antibodies in rabbits. Purified anti-CP-IgG detected the BNYVV-CP recombinant protein and BNYVV in infected sugar beet by indirect-ELISA, dot immunobinding assay, and western blot analysis blotting. The serological assays strongly proved the sensitivity and specificity of anti-BNYVV-CP-IgG against BNYVV. These results suggest that the generated anti-BNYVV-CP-IgG polyclonal antibodies can be used as reliable and quick means for the BNYVV virus detection under field conditions.
    Keywords Beet necrotic yellow vein virus ; Escherichia coli ; Western blotting ; affinity chromatography ; antigens ; coat proteins ; genes ; genomics ; plant pathology ; plant protection ; polyclonal antibodies ; recombinant proteins ; rhizomania ; sugar beet ; viruses ; BNYVV virus ; recombinant protein ; ELISA
    Language English
    Dates of publication 2023-0102
    Size p. 10-28.
    Publishing place Taylor & Francis
    Document type Article ; Online
    ZDB-ID 2068307-8
    ISSN 1477-2906 ; 0323-5408
    ISSN (online) 1477-2906
    ISSN 0323-5408
    DOI 10.1080/03235408.2023.2170205
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  2. Article: Plant-derived VLP: a worthy platform to produce vaccine against SARS-CoV-2

    Hemmati, Farshad / Hemmati-Dinarvand, Mohsen / Karimzade, Marziye / Rutkowska, Daria / Eskandari, Mohammad Hadi / Khanizadeh, Sayyad / Afsharifar, Alireza

    Biotechnology letters. 2022 Jan., v. 44, no. 1

    2022  

    Abstract: After its emergence in late 2019 SARS-CoV-2 was declared a pandemic by the World Health Organization on 11 March 2020 and has claimed more than 2.8 million lives. There has been a massive global effort to develop vaccines against SARS-CoV-2 and the rapid ...

    Abstract After its emergence in late 2019 SARS-CoV-2 was declared a pandemic by the World Health Organization on 11 March 2020 and has claimed more than 2.8 million lives. There has been a massive global effort to develop vaccines against SARS-CoV-2 and the rapid and low cost production of large quantities of vaccine is urgently needed to ensure adequate supply to both developed and developing countries. Virus-like particles (VLPs) are composed of viral antigens that self-assemble into structures that mimic the structure of native viruses but lack the viral genome. Thus they are not only a safer alternative to attenuated or inactivated vaccines but are also able to induce potent cellular and humoral immune responses and can be manufactured recombinantly in expression systems that do not require viral replication. VLPs have successfully been produced in bacteria, yeast, insect and mammalian cell cultures, each production platform with its own advantages and limitations. Plants offer a number of advantages in one production platform, including proper eukaryotic protein modification and assembly, increased safety, low cost, high scalability as well as rapid production speed, a critical factor needed to control outbreaks of potential pandemics. Plant-based VLP-based viral vaccines currently in clinical trials include, amongst others, Hepatitis B virus, Influenza virus and SARS-CoV-2 vaccines. Here we discuss the importance of plants as a next generation expression system for the fast, scalable and low cost production of VLP-based vaccines.
    Keywords Hepatitis B virus ; Orthomyxoviridae ; Severe acute respiratory syndrome coronavirus 2 ; World Health Organization ; biotechnology ; insects ; mammals ; pandemic ; viral genome ; virus replication ; yeasts
    Language English
    Dates of publication 2022-01
    Size p. 45-57.
    Publishing place Springer Netherlands
    Document type Article
    Note Review
    ZDB-ID 423853-9
    ISSN 1573-6776 ; 0141-5492
    ISSN (online) 1573-6776
    ISSN 0141-5492
    DOI 10.1007/s10529-021-03211-0
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4564: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Identification and characterization of a phytoplasma associated with black locust yellow disease in two provinces of Iran

    Karimzade, Marziye / Siampour, Majid / Zakiaghl, Mohammad / Mehrvar, Mohsen

    Crop protection. 2018 Aug., v. 110

    2018  

    Abstract: A phytoplasma disease was associated with black locust trees showing yellows, a devastating disease found in different geographical areas of Iran. Infected trees showed yellowing, reduced leaf size, short internodes and proliferation of buds, dieback and ...

    Abstract A phytoplasma disease was associated with black locust trees showing yellows, a devastating disease found in different geographical areas of Iran. Infected trees showed yellowing, reduced leaf size, short internodes and proliferation of buds, dieback and decline. The disease was transmitted to healthy black locust and periwinkle plants in greenhouse. DNA extractions were made from midrib of leaf tissues. Nested PCR assays with R16mF2/R16mR1 universal phytoplasma primers detected a 1.4 kb product and an 850 bp product was obtained using Fu5/Ru3 primers in double nested PCR. Phytoplasmas were detected from all symptomatic black locust trees as well as inoculated black locust and periwinkle plants. Analysis of 16S rRNA gene sequences showed that phytoplasmas associated with black locust yellows (BLYp) were related to ‘Candidatus Phytoplasma phoenicium’ (group 16SrIX). Moreover, four 16S rDNA genetic isolates (BLYp-a to -d), distinguished by 1–10 single nucleotide polymorphisms (SNPs), were identified in BLYp population. Virtual RFLP analysis determined that BLYp-a represents a new tentative subgroup within the 16SrIX group; BLYp-b, BLYp-c and BLYp-d were assigned to subgroup 16SrIX-C. Actual RFLP profiles derived from EcoRI, RsaI, HinfI and TaqI enzymes confirmed virtual RFLP subgroup characterization. Phylogenetic analysis showed that BLYp isolates shared a recent common ancestor with some 16SrIX-C phytoplasma strains. Moreover, comparisons of 16S rRNA and SecY gene nucleotide sequence raised the question as to whether BLYp and other 16SrIX-C phytoplasmas identified in Iran are identical or closely related strains. To the best of our knowledge, this is the first report of 16SrIX phytoplasmas infecting black locust.
    Keywords Phytoplasma ; Robinia pseudoacacia ; buds ; decline ; dieback ; enzymes ; genes ; greenhouses ; internodes ; leaves ; nucleotide sequences ; phylogeny ; phytoplasmal diseases ; plant pathogenic bacteria ; polymerase chain reaction ; restriction fragment length polymorphism ; ribosomal DNA ; ribosomal RNA ; single nucleotide polymorphism ; tissues ; tree diseases ; trees ; Iran
    Language English
    Dates of publication 2018-08
    Size p. 261-268.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 786839-x
    ISSN 1873-6904 ; 0261-2194
    ISSN (online) 1873-6904
    ISSN 0261-2194
    DOI 10.1016/j.cropro.2017.05.010
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    In stock of ZB MED Bonn / Germany

    Z 3919: Show issues

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  4. Article ; Online: Plant-derived VLP: a worthy platform to produce vaccine against SARS-CoV-2.

    Hemmati, Farshad / Hemmati-Dinarvand, Mohsen / Karimzade, Marziye / Rutkowska, Daria / Eskandari, Mohammad Hadi / Khanizadeh, Sayyad / Afsharifar, Alireza

    Biotechnology letters

    2021  Volume 44, Issue 1, Page(s) 45–57

    Abstract: After its emergence in late 2019 SARS-CoV-2 was declared a pandemic by the World Health Organization on 11 March 2020 and has claimed more than 2.8 million lives. There has been a massive global effort to develop vaccines against SARS-CoV-2 and the rapid ...

    Abstract After its emergence in late 2019 SARS-CoV-2 was declared a pandemic by the World Health Organization on 11 March 2020 and has claimed more than 2.8 million lives. There has been a massive global effort to develop vaccines against SARS-CoV-2 and the rapid and low cost production of large quantities of vaccine is urgently needed to ensure adequate supply to both developed and developing countries. Virus-like particles (VLPs) are composed of viral antigens that self-assemble into structures that mimic the structure of native viruses but lack the viral genome. Thus they are not only a safer alternative to attenuated or inactivated vaccines but are also able to induce potent cellular and humoral immune responses and can be manufactured recombinantly in expression systems that do not require viral replication. VLPs have successfully been produced in bacteria, yeast, insect and mammalian cell cultures, each production platform with its own advantages and limitations. Plants offer a number of advantages in one production platform, including proper eukaryotic protein modification and assembly, increased safety, low cost, high scalability as well as rapid production speed, a critical factor needed to control outbreaks of potential pandemics. Plant-based VLP-based viral vaccines currently in clinical trials include, amongst others, Hepatitis B virus, Influenza virus and SARS-CoV-2 vaccines. Here we discuss the importance of plants as a next generation expression system for the fast, scalable and low cost production of VLP-based vaccines.
    MeSH term(s) Antigens, Viral/genetics ; Antigens, Viral/metabolism ; COVID-19 Vaccines/biosynthesis ; COVID-19 Vaccines/economics ; COVID-19 Vaccines/genetics ; Gene Expression ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; SARS-CoV-2/immunology ; Vaccines, Virus-Like Particle/biosynthesis ; Vaccines, Virus-Like Particle/economics ; Vaccines, Virus-Like Particle/genetics ; Viral Vaccines/biosynthesis ; Viral Vaccines/genetics
    Chemical Substances Antigens, Viral ; COVID-19 Vaccines ; Recombinant Proteins ; Vaccines, Virus-Like Particle ; Viral Vaccines
    Language English
    Publishing date 2021-11-27
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 423853-9
    ISSN 1573-6776 ; 0141-5492
    ISSN (online) 1573-6776
    ISSN 0141-5492
    DOI 10.1007/s10529-021-03211-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4564: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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