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  1. AU="Karine Bernardeau"
  2. AU="Post, Zachary D"
  3. AU="Mukherjee, Prattusha"
  4. AU="Yang, Wulin"
  5. AU=Jang Kyoung-Min
  6. AU="Shilpakar, Ramila"
  7. AU="Azad, Sajad"
  8. AU="Connolly, John A"
  9. AU="Šmit, Žiga"
  10. AU=Lee Geun Dong
  11. AU="Laireiter, Anton-Rupert"
  12. AU=Allen Larry A
  13. AU="Jamali, Myriam"
  14. AU="Lima-Filho, Moysés de Oliveira"
  15. AU="Bowen, Ceri"
  16. AU=Demidenko Eugene
  17. AU="Hashemi-Soteh, Mohammad Bagher"
  18. AU="Monfardini, Silvio"
  19. AU="Nasim, Aqeel"
  20. AU=Stennard Fiona A

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  1. Artikel ; Online: A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening

    Juliette Vaurs / Gaël Douchin / Klara Echasserieau / Romain Oger / Nicolas Jouand / Agnès Fortun / Leslie Hesnard / Mikaël Croyal / Frédéric Pecorari / Nadine Gervois / Karine Bernardeau

    Scientific Reports, Vol 11, Iss 1, Pp 1-

    2021  Band 12

    Abstract: Abstract Over the past two decades, there has been a great interest in the study of HLA-E-restricted αβ T cells during bacterial and viral infections, including recently SARS-CoV-2 infection. Phenotyping of these specific HLA-E-restricted T cells ... ...

    Abstract Abstract Over the past two decades, there has been a great interest in the study of HLA-E-restricted αβ T cells during bacterial and viral infections, including recently SARS-CoV-2 infection. Phenotyping of these specific HLA-E-restricted T cells requires new tools such as tetramers for rapid cell staining or sorting, as well as for the identification of new peptides capable to bind to the HLA-E pocket. To this aim, we have developed an optimal photosensitive peptide to generate stable HLA-E/pUV complexes allowing high-throughput production of new HLA-E/peptide complexes by peptide exchange. We characterized the UV exchange by ELISA and improved the peptide exchange readout using size exclusion chromatography. This novel approach for complex quantification is indeed very important to perform tetramerization of MHC/peptide complexes with the high quality required for detection of specific T cells. Our approach allows the rapid screening of peptides capable of binding to the non-classical human HLA-E allele, paving the way for the development of new therapeutic approaches based on the detection of HLA-E-restricted T cells.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 540
    Sprache Englisch
    Erscheinungsdatum 2021-08-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Cross-Reactive Donor-Specific CD8+ Tregs Efficiently Prevent Transplant Rejection

    Elodie Picarda / Séverine Bézie / Lorena Usero / Jason Ossart / Marine Besnard / Hanim Halim / Klara Echasserieau / Claire Usal / Jamie Rossjohn / Karine Bernardeau / Stéphanie Gras / Carole Guillonneau

    Cell Reports, Vol 29, Iss 13, Pp 4245-4255.e

    2019  Band 6

    Abstract: Summary: To reduce the use of non-specific immunosuppressive drugs detrimental to transplant patient health, therapies in development aim to achieve antigen-specific tolerance by promoting antigen-specific regulatory T cells (Tregs). However, ... ...

    Abstract Summary: To reduce the use of non-specific immunosuppressive drugs detrimental to transplant patient health, therapies in development aim to achieve antigen-specific tolerance by promoting antigen-specific regulatory T cells (Tregs). However, identification of the natural antigens recognized by Tregs and the contribution of their dominance in transplantation has been challenging. We identify epitopes derived from distinct major histocompatibility complex (MHC) class II molecules, sharing a 7-amino acid consensus sequence positioned in a central mobile section in complex with MHC class I, recognized by cross-reactive CD8+ Tregs, enriched in the graft. Antigen-specific CD8+ Tregs can be induced in vivo with a 16-amino acid-long peptide to trigger transplant tolerance. Peptides derived from human HLA class II molecules, harboring the rat consensus sequence, also activate and expand human CD8+ Tregs, suggesting its potential in human transplantation. Altogether, this work should facilitate the development of therapies with peptide epitopes for transplantation and improve our understanding of CD8+ Treg recognition. : Picarda et al. describe MHC class II-derived peptides recognized by cross-reactive CD8+ Tregs instrumental for tolerance induction in transplantation between an incompatible donor and recipient. Keywords: transplantation, tolerance, peptide, CD8+, rat, human, therapy, regulation, antigen-specific
    Schlagwörter Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2019-12-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Histo-blood group antigen-binding specificities of human rotaviruses are associated with gastroenteritis but not with in vitro infection

    Laure Barbé / Béatrice Le Moullac-Vaidye / Klara Echasserieau / Karine Bernardeau / Thomas Carton / Nicolai Bovin / Johan Nordgren / Lennart Svensson / Nathalie Ruvoën-Clouet / Jacques Le Pendu

    Scientific Reports, Vol 8, Iss 1, Pp 1-

    2018  Band 14

    Abstract: Abstract Human strains of rotavirus A (RVAs) recognize fucosylated glycans belonging to histo-blood group antigens (HBGAs) through their spike protein VP8*. Lack of these ligands due to genetic polymorphisms is associated with resistance to ... ...

    Abstract Abstract Human strains of rotavirus A (RVAs) recognize fucosylated glycans belonging to histo-blood group antigens (HBGAs) through their spike protein VP8*. Lack of these ligands due to genetic polymorphisms is associated with resistance to gastroenteritis caused by P[8] genotype RVAs. With the aim to delineate the contribution of HBGAs in the process, we analyzed the glycan specificity of VP8* proteins from various P genotypes. Binding to saliva of VP8* from P[8] and P[4] genotypes required expression of both FUT2 and FUT3 enzymes, whilst binding of VP8* from the P[14] genotype required FUT2 and A enzymes. We further defined a glycan motif, GlcNAcβ3Galβ4GlcNAc, recognized by P[6] clinical strains. Conversion into Lewis antigens by the FUT3 enzyme impaired recognition, explaining their lower binding to saliva of Lewis positive phenotype. In addition, the presence of neutralizing antibodies was associated with the presence of the FUT2 wild type allele in sera from young healthy adults. Nonetheless, in vitro infection of transformed cell lines was independent of HBGAs expression, indicating that HBGAs are not human RV receptors. The match between results from saliva-based binding assays and the epidemiological data indicates that the polymorphism of human HBGAs controls susceptibility to RVAs, although the exact mechanism remains unclear.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 570
    Sprache Englisch
    Erscheinungsdatum 2018-08-01T00:00:00Z
    Verlag Nature Publishing Group
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Serum soluble HLA-E in melanoma

    Mathilde Allard / Romain Oger / Virginie Vignard / Jean-Michel Percier / Giulia Fregni / Aurélie Périer / Anne Caignard / Béatrice Charreau / Karine Bernardeau / Amir Khammari / Brigitte Dréno / Nadine Gervois

    PLoS ONE, Vol 6, Iss 6, p e

    a new potential immune-related marker in cancer.

    2011  Band 21118

    Abstract: Background Tumor-derived soluble factors, including soluble HLA molecules, can contribute to cancer immune escape and therefore impact on clinical course of malignant diseases. We previously reported that melanoma cells produce, in vitro, soluble forms ... ...

    Abstract Background Tumor-derived soluble factors, including soluble HLA molecules, can contribute to cancer immune escape and therefore impact on clinical course of malignant diseases. We previously reported that melanoma cells produce, in vitro, soluble forms of the non-classical MHC class I molecule HLA-E (sHLA-E). In order to investigate sHLA-E production by various tumors and to address its potential value as a tumor-associated marker, we developed a specific ELISA for the quantification of sHLA-E in biological fluids. Methodology/principal findings We developed a sHLA-E specific and sensitive ELISA and we showed that serum sHLA-E levels were significantly elevated (P<0.01) in melanoma patients (n = 127), compared with healthy donors (n = 94). sHLA-E was also detected in the culture supernatants of a wide variety of tumor cell lines (n = 98) including melanomas, kidney, colorectal and breast cancers. Cytokines regulation of sHLA-E production by tumor cells was also carried out. IFN-γ, IFN-α and TNF-α were found to upregulate sHLA-E production by tumor cells. Conclusions/significance In view of the broad tumor tissue release of HLA-E and its up-regulation by inflammatory cytokines, sHLA-E should be studied for its involvement in immune responses against tumors. Interestingly, our results demonstrated a positive association between the presence of serum sHLA-E and melanoma. Therefore, the determination of sHLA-E levels, using ELISA approach, may be investigated as a clinical marker in cancer patients.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2011-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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