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  1. Article ; Online: Elemental Bioimaging of Sheep Bone and Articular Cartilage After Single Application of Gadolinium-Based Contrast Agents.

    Richter, Henning / Verlemann, Christine / Jeibmann, Astrid / Martin, Louise F / Luebke, Andreas M / Karol, Agnieszka / Sperling, Michael / Radbruch, Alexander / Karst, Uwe

    Investigative radiology

    2023  Volume 59, Issue 4, Page(s) 287–292

    Abstract: Background: Gadolinium-based contrast agents (GBCAs) are applied to enhance magnetic resonance imaging. Gadolinium (Gd), a rare earth metal, is used in a chelated form when administered as GBCA to patients. There is an ongoing scientific debate about ... ...

    Abstract Background: Gadolinium-based contrast agents (GBCAs) are applied to enhance magnetic resonance imaging. Gadolinium (Gd), a rare earth metal, is used in a chelated form when administered as GBCA to patients. There is an ongoing scientific debate about the clinical significance of Gd retention in tissues after administration of GBCAs. It is known that bone serves as Gd reservoir, but only sparse information on localization of Gd in bone is available.
    Purpose: The aim of this study was to compare Gd tissue concentration and spatial distribution in femoral epiphysis and diaphysis 10 weeks after single-dose injection of linear and macrocyclic GBCAs in a large animal model.
    Materials and methods: In this prospective animal study, Swiss-Alpine sheep (n = 36; age range, 4-10 years) received a single injection (0.1 mmol/kg) of macrocyclic (gadobutrol, gadoteridol, and gadoterate meglumine), linear (gadodiamide and gadobenate dimeglumine) GBCAs, or saline. Ten weeks after injection, sheep were killed, and femur heads and shafts were harvested. Gadolinium spatial distribution was determined in 1 sample of each treatment group by laser ablation-inductively coupled plasma-mass spectrometry. All bone specimens were analyzed histopathologically.
    Results: Injection of GBCAs in female Swiss-Alpine sheep (n = 36) resulted in Gd localization at the endosteal and periosteal surface and in a subset of GBCAs additionally at the cement lines and the bone cartilage junction. No histopathological alterations were observed in the investigated tissue specimens.
    Conclusions: Ten weeks after single injection of a clinically relevant dose in adult sheep, both linear species of GBCA resulted in considerably higher accumulation than macrocyclic GBCAs. Gadolinium deposits were restricted to distinct bone and cartilage compartments, such as in bone linings, cement lines, and bone cartilage junctions. Tissue histology remained unaffected.
    MeSH term(s) Humans ; Female ; Animals ; Sheep ; Child, Preschool ; Child ; Contrast Media ; Gadolinium ; Cartilage, Articular/diagnostic imaging ; Prospective Studies ; Organometallic Compounds ; Gadolinium DTPA ; Magnetic Resonance Imaging/methods ; Brain
    Chemical Substances Contrast Media ; Gadolinium (AU0V1LM3JT) ; Organometallic Compounds ; Gadolinium DTPA (K2I13DR72L)
    Language English
    Publishing date 2023-09-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80345-5
    ISSN 1536-0210 ; 0020-9996
    ISSN (online) 1536-0210
    ISSN 0020-9996
    DOI 10.1097/RLI.0000000000001020
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  2. Article ; Online: Effect of PARP-1 Inhibition on Rotator Cuff Healing: A Feasibility Study Using Veliparib in a Rat Model of Acute Rotator Cuff Repair.

    Künzler, Michael B / McGarry, Michelle H / Akeda, Masaki / Ihn, Hansel / Karol, Agnieszka / von Rechenberg, Brigitte / Schär, Michael O / Zumstein, Matthias A / Lee, Thay Q

    The American journal of sports medicine

    2023  Volume 51, Issue 3, Page(s) 758–767

    Abstract: Background: PARP-1 (poly[ADP-ribose]) was shown to influence the inflammatory response after rotator cuff tear, leading to fibrosis, muscular atrophy, and fatty infiltration in mouse rotator cuff degeneration. So far, it is not known how PARP-1 ... ...

    Abstract Background: PARP-1 (poly[ADP-ribose]) was shown to influence the inflammatory response after rotator cuff tear, leading to fibrosis, muscular atrophy, and fatty infiltration in mouse rotator cuff degeneration. So far, it is not known how PARP-1 influences enthesis healing after rotator cuff tear repair.
    Hypothesis/purpose: This study aimed to examine the feasibility of oral PARP-1 inhibition and investigate its influence on rat supraspinatus enthesis and muscle healing after rotator cuff repair. The hypothesis was that oral PARP-1 inhibition would improve enthesis healing after acute rotator cuff repair in a rat model.
    Study design: Controlled laboratory study.
    Methods: In 24 Sprague-Dawley rats, the supraspinatus tendon was sharply detached and immediately repaired with a single transosseous suture. The rats were randomly allocated into 2 groups, with the rats in the inhibitor group receiving veliparib with a target dose of 12.5 mg/kg/d via drinking water during the postoperative recovery period. The animals were sacrificed 8 weeks after surgery. For the analysis, macroscopic, biomechanical, and histologic methods were used.
    Results: Oral veliparib was safe for the rats, with no adverse effects observed. In total, the inhibitor group had a significantly better histologic grading of the enthesis with less scar tissue formation. The macroscopic cross-sectional area of the supraspinatus muscles was 10.5% higher (
    Conclusion: This study is the first to investigate the influence of PARP-1 inhibition on healing enthesis. On the basis of these findings, we conclude that oral veliparib, which was previously shown to inhibit PARP-1 effectively, is safe to apply and has beneficial effects on morphologic enthesis healing and muscle fiber size.
    Clinical relevance: Modulating the inflammatory response through PARP-1 inhibition during the postoperative healing period is a promising approach to improve enthesis healing and reduce rotator cuff retearing. With substances already approved by the Food and Drug Administration, PARP-1 inhibition bears high potential for future translation into clinical application.
    MeSH term(s) Rats ; Mice ; Animals ; Rotator Cuff/pathology ; Rotator Cuff Injuries/drug therapy ; Rotator Cuff Injuries/surgery ; Rotator Cuff Injuries/pathology ; Wound Healing/physiology ; Feasibility Studies ; Poly(ADP-ribose) Polymerase Inhibitors/pharmacology ; Poly(ADP-ribose) Polymerase Inhibitors/therapeutic use ; Rats, Sprague-Dawley ; Biomechanical Phenomena
    Chemical Substances veliparib (01O4K0631N) ; Poly(ADP-ribose) Polymerase Inhibitors
    Language English
    Publishing date 2023-02-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 197482-8
    ISSN 1552-3365 ; 0363-5465
    ISSN (online) 1552-3365
    ISSN 0363-5465
    DOI 10.1177/03635465221148494
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  3. Article ; Online: Establishment of a localized acute implant-associated Staphylococcus aureus bone infection model in sheep.

    Klein, Karina / Schweizer, Tiziano A / Siwy, Katharina / Lechmann, Beat / Karol, Agnieszka / von Rechenberg, Brigitte / Achermann, Yvonne / Darwiche, Salim E

    Pathogens and disease

    2021  Volume 79, Issue 6

    Abstract: Orthopedic implant-associated bacterial infections with Staphylococcus aureus constitute a major clinical problem, and large pre-clinical animal models remain scarce. The aim of this study was to establish a standardized method of a localized, acute S. ... ...

    Abstract Orthopedic implant-associated bacterial infections with Staphylococcus aureus constitute a major clinical problem, and large pre-clinical animal models remain scarce. The aim of this study was to establish a standardized method of a localized, acute S. aureus bone infection in the presence of complex implanted devices in a sheep model. Four sheep underwent surgery receiving a complex implanted metallic device with a component stabilizing a bone defect created in the left tibial metaphysis, and an attached component placed in adjacent soft tissue. The bone defect was inoculated with S. aureus strain ATCC25293 (1 × 104 CFU). Twenty one days later, the surgery site was macroscopically evaluated, tissue samples and implants harvested for bacterial cell count quantification and tissue samples histologically analyzed. The animals exhibited clinical signs of localized infection (e.g. swelling, lameness, pain) but did not develop symptoms of sepsis. After euthanasia, macroscopic assessment revealed a localized bone and soft tissue infection at the surgery site. Histologically, an acute inflammation with neutrophils but also signs of bone destruction with necrosis was noted. An ovine model of a localized, acute S. aureus bone infection with complex implants was successfully established and could be used to test novel treatments against orthopedic implant-associated infections.
    MeSH term(s) Animals ; Biofilms/growth & development ; Disease Models, Animal ; Humans ; Osteomyelitis/diagnostic imaging ; Osteomyelitis/microbiology ; Osteomyelitis/pathology ; Prostheses and Implants/microbiology ; Prosthesis-Related Infections/microbiology ; Sheep ; Staphylococcal Infections/diagnostic imaging ; Staphylococcal Infections/microbiology ; Staphylococcal Infections/pathology ; Staphylococcus aureus
    Language English
    Publishing date 2021-01-31
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2049-632X
    ISSN (online) 2049-632X
    DOI 10.1093/femspd/ftab032
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  4. Article ; Online: Studying Edema Formation After Release of the Infraspinatus Tendon as an Experimental Model of Rotator Cuff Tears in Sheep: A Preliminary Imaging and Morphological Analysis.

    Lädermann, Alexandre / Gehrke, Rieke / Klein, Karina / Karol, Agnieszka / Darwiche, Salim / Schwarzenberg, Peter / Steffen, Thomas / Wieser, Karl / Kronen, Peter / von Rechenberg, Brigitte

    The American journal of sports medicine

    2022  Volume 50, Issue 14, Page(s) 3934–3940

    Abstract: Background: The cause, extent, and role of muscle edema for muscle degeneration are unknown and not considered in the current literature. In vivo experiments were designed to prove muscle edema formation in the early period in a sheep model of acute ... ...

    Abstract Background: The cause, extent, and role of muscle edema for muscle degeneration are unknown and not considered in the current literature. In vivo experiments were designed to prove muscle edema formation in the early period in a sheep model of acute rotator cuff tears.
    Hypothesis: Muscle edema occurs after tendon release with or without additional stretching trauma and may be associated with muscle retraction and subsequent muscle degeneration.
    Study design: Controlled laboratory study.
    Methods: A sheep model with acute release of the infraspinatus tendon was used. An osteotomy of the greater tuberosity, including the insertion of the infraspinatus tendon, was performed in 14 sheep. To demonstrate presence of edema, magnetic resonance imaging scans were performed at 0, 2, and 4 weeks using T1-weighted, T2-weighted, proton density-weighted, and Dixon sequences. Excisional biopsy specimens were taken at 0, 3, and 4 weeks (histological results will be reported in a later publication). Two injury models were created: a nontrauma group that consisted of muscle release alone and a trauma group that included additional standardized traction to the musculotendinous unit. Evaluation of T1- and T2-weighted images included calculation of pennation angle, muscle fiber length, signal intensity (edema), and muscle volume. Muscle wet weight and volume were measured at sacrifice.
    Results: Edema formation was shown in all sheep and slightly more pronounced in the trauma group, where muscle intensity increased significantly between time point 0 (200 Grey Value (GV)) and weeks 2, 3, and 4 (300 GV). Edema formation started early after tendon release with a plateau between 3 and 4 weeks. Deterioration of muscle fiber bundles began also after tendon release with a peak at 4 weeks. Muscle volume decreased steadily over time.
    Conclusion: Muscle edema appeared early after rotator cuff tendon release, was more pronounced in the trauma group, and reached a plateau after 3 to 4 weeks. Muscle fatty content decreased within the short period of 4 weeks owing to a dilution effect. Muscle edema seems to be an essential factor in cuff tears and subsequent muscle retraction and degeneration.
    Clinical relevance: This study demonstrates a new type of muscle edema of retraction and describes the characteristics of edema associated with a retracted rotator cuff tear.
    MeSH term(s) Sheep ; Animals ; Research Design ; Social Group ; Models, Theoretical
    Language English
    Publishing date 2022-11-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 197482-8
    ISSN 1552-3365 ; 0363-5465
    ISSN (online) 1552-3365
    ISSN 0363-5465
    DOI 10.1177/03635465221130446
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  5. Article ; Online: CD90-positive stromal cells associate with inflammatory and fibrotic changes in modic changes.

    Dudli, Stefan / Karol, Agnieszka / Giudici, Luca / Heggli, Irina / Laux, Christoph J / Spirig, Jose M / Wanivenhaus, Florian / Betz, Michael / Germann, Christoph / Farshad-Amacker, Nadja / Brunner, Florian / Distler, Oliver / Farshad, Mazda

    Osteoarthritis and cartilage open

    2022  Volume 4, Issue 3, Page(s) 100287

    Abstract: Objective: Modic changes (MC) are vertebral bone marrow lesions seen on magnetic resonance images, that associate with disc degeneration and low back pain (LBP). Few studies described MC histopathology qualitatively based on a few patient samples. CD90- ... ...

    Abstract Objective: Modic changes (MC) are vertebral bone marrow lesions seen on magnetic resonance images, that associate with disc degeneration and low back pain (LBP). Few studies described MC histopathology qualitatively based on a few patient samples. CD90-positive bone marrow stromal cells were shown to be pro-fibrotic in MC. We aimed to provide the first semi-quantitative histomorphometric analysis of MC bone marrow. We hypothesized a role of CD90-positive cells in MC pathomechanisms.
    Design: Human biopsies from Modic type 1 changes (MC1, n ​= ​8), Modic type 2 changes (MC2, n ​= ​6), and control biopsies (MC0, n ​= ​8) from adjacent vertebrae were obtained from 14 LBP patients during lumbar spinal fusion. Biopsies were processed for histology/immunohistochemistry. Inflammatory changes (oedema, inflammatory infiltrates), fibrotic changes (connective tissue, type I and III collagen, fibronectin, α-smooth muscle actin), and amount of bone marrow stromal cells (CD90, CD105) were scored. Scores for MC0, MC1, and MC2 were compared with non-parametric tests. Pairwise correlations, hierarchical clustering, and principal component analysis of histological readouts were calculated to identify most important histomorphometric MC characteristics.
    Results: Compared to MC0, MC1 had more connective tissue, oedema, inflammatory infiltrates, and CD90
    Conclusion: Accumulation of CD90
    Language English
    Publishing date 2022-06-22
    Publishing country England
    Document type Journal Article
    ISSN 2665-9131
    ISSN (online) 2665-9131
    DOI 10.1016/j.ocarto.2022.100287
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  6. Article ; Online: Platelet-rich plasma as a potential prophylactic measure against frozen shoulder in an in vivo shoulder contracture model.

    Feusi, Oscar / Karol, Agnieszka / Fleischmann, Thea / von Rechenberg, Brigitte / Bouaicha, Samy / Werner, Clément M L / Jentzsch, Thorsten

    Archives of orthopaedic and trauma surgery

    2020  Volume 142, Issue 3, Page(s) 363–372

    Abstract: Introduction: Frozen shoulder (adhesive capsulitis) is a common painful and functionally-limiting disease affecting around 2% of the population. So far, therapeutic options are limited and often unsatisfactory. Platelet-rich plasma (PRP) has been used ... ...

    Abstract Introduction: Frozen shoulder (adhesive capsulitis) is a common painful and functionally-limiting disease affecting around 2% of the population. So far, therapeutic options are limited and often unsatisfactory. Platelet-rich plasma (PRP) has been used as a treatment option in other orthopedic diseases since it contains growth factors that stimulate tissue repair. So far, the effect of PRP on frozen shoulder lacks evidence. We hypothesized that PRP may be valuable in the prophylaxis and treatment of secondary frozen shoulder due to capsular remodeling.
    Materials and methods: An experimental study of an in vivo frozen shoulder model was conducted. Twenty Sprague-Dawley rats underwent surgery in which the body of the scapula was connected to the humerus with a high-strength suture. Two groups of 8 weeks survival time were allocated; a treatment group with one intraoperative injection of PRP into the glenohumeral joint (n = 10) and a control group without PRP (n = 10). The primary outcome was the structural change in the posterior synovial membrane of the posterior and inferior part of the glenohumeral joint using a semi-quantitative grading from 0 (lowest) to 3 (highest).
    Results: The posterior synovial membrane structural changes were significantly lower in the PRP group (median = 1 [interquartile range (IQR) = 0-1]) compared to controls (median = 2 [IQR = 1-3]) (p = 0.028). There were no differences for the remaining synovial membrane changes and fibrous capsule responses between groups.
    Conclusions: In this in vivo shoulder contracture model, PRP injections seem to reduce the histological severity grade of some parts (i.e., posterior synovial membrane changes) of the secondary frozen shoulder without causing any side effects. It may be considered to investigate this effect further in future studies as a potential prophylaxis of secondary frozen shoulder (e.g., in operated or immobilized shoulders) or as a treatment option for patients with frozen shoulder in the early stage.
    MeSH term(s) Animals ; Bursitis/therapy ; Contracture/prevention & control ; Humans ; Platelet-Rich Plasma ; Rats ; Rats, Sprague-Dawley ; Shoulder ; Shoulder Joint
    Language English
    Publishing date 2020-10-19
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 80407-1
    ISSN 1434-3916 ; 0003-9330 ; 0344-8444
    ISSN (online) 1434-3916
    ISSN 0003-9330 ; 0344-8444
    DOI 10.1007/s00402-020-03617-x
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    Uk I Zs.58: Show issues Location:
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  7. Book ; Thesis: Charakterisierung von Kadidatengenen für Tropfsaftverlust im Schweinefleisch

    Karol, Agnieszka Anna

    2009  

    Author's details vorgelegt von Agnieszka Anna Karol
    Language German ; English
    Size 23 Bl, graph. Darst
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., Vetsuisse-Fak., Diss.--Bern, 2009
    Database Special collection on veterinary medicine and general parasitology

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  8. Article ; Online: Premature aging in mice with error-prone protein synthesis.

    Shcherbakov, Dimitri / Nigri, Martina / Akbergenov, Rashid / Brilkova, Margarita / Mantovani, Matilde / Petit, Patricia Isnard / Grimm, Amandine / Karol, Agnieszka A / Teo, Youjin / Sanchón, Adrián Cortés / Kumar, Yadhu / Eckert, Anne / Thiam, Kader / Seebeck, Petra / Wolfer, David P / Böttger, Erik C

    Science advances

    2022  Volume 8, Issue 9, Page(s) eabl9051

    Abstract: The main source of error in gene expression is messenger RNA decoding by the ribosome. Translational accuracy has been suggested on a purely correlative basis to positively coincide with maximum possible life span among different rodent species, but ... ...

    Abstract The main source of error in gene expression is messenger RNA decoding by the ribosome. Translational accuracy has been suggested on a purely correlative basis to positively coincide with maximum possible life span among different rodent species, but causal evidence that translation errors accelerate aging in vivo and limit life span is lacking. We have now addressed this question experimentally by creating heterozygous knock-in mice that express the ribosomal ambiguity mutation RPS9 D95N, resulting in genome-wide error-prone translation. Here, we show that
    MeSH term(s) Aging/genetics ; Aging/metabolism ; Aging, Premature/genetics ; Animals ; Longevity ; Mammals/genetics ; Mice ; Reactive Oxygen Species ; Telomere
    Chemical Substances Reactive Oxygen Species
    Language English
    Publishing date 2022-03-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.abl9051
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  9. Article ; Online: Modic type 2 changes are fibroinflammatory changes with complement system involvement adjacent to degenerated vertebral endplates.

    Heggli, Irina / Laux, Christoph J / Mengis, Tamara / Karol, Agnieszka / Cornaz, Frédéric / Herger, Nick / Aradi-Vegh, Borbala / Widmer, Jonas / Burkhard, Marco D / Farshad-Amacker, Nadja A / Pfammatter, Sibylle / Wolski, Witold E / Brunner, Florian / Distler, Oliver / Farshad, Mazda / Dudli, Stefan

    JOR spine

    2022  Volume 6, Issue 1, Page(s) e1237

    Abstract: Background: Vertebral endplate signal intensity changes visualized by magnetic resonance imaging termed Modic changes (MC) are highly prevalent in low back pain patients. Interconvertibility between the three MC subtypes (MC1, MC2, MC3) suggests ... ...

    Abstract Background: Vertebral endplate signal intensity changes visualized by magnetic resonance imaging termed Modic changes (MC) are highly prevalent in low back pain patients. Interconvertibility between the three MC subtypes (MC1, MC2, MC3) suggests different pathological stages. Histologically, granulation tissue, fibrosis, and bone marrow edema are signs of inflammation in MC1 and MC2. However, different inflammatory infiltrates and amount of fatty marrow suggest distinct inflammatory processes in MC2.
    Aims: The aims of this study were to investigate (i) the degree of bony (BEP) and cartilage endplate (CEP) degeneration in MC2, (ii) to identify inflammatory MC2 pathomechanisms, and (iii) to show that these marrow changes correlate with severity of endplate degeneration.
    Methods: Pairs of axial biopsies (
    Results: Endplates from MC2 were significantly more degenerated. Proteomic analysis revealed an activated complement system, increased expression of extracellular matrix proteins, angiogenic, and neurogenic factors in MC2 marrow. Endplate scores correlated with upregulated complement and neurogenic proteins.
    Discussion: The inflammatory pathomechanisms in MC2 comprises activation of the complement system. Concurrent inflammation, fibrosis, angiogenesis, and neurogenesis indicate that MC2 is a chronic inflammation. Correlation of endplate damage with complement and neurogenic proteins suggest that complement system activation and neoinnervation may be linked to endplate damage. The endplate-near marrow is the pathomechanistic site, because MC2 occur at locations with more endplate degeneration.
    Conclusion: MC2 are fibroinflammatory changes with complement system involvement which occur adjacent to damaged endplates.
    Language English
    Publishing date 2022-12-23
    Publishing country United States
    Document type Journal Article
    ISSN 2572-1143
    ISSN (online) 2572-1143
    DOI 10.1002/jsp2.1237
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  10. Article ; Online: MRI-guided, transrectal, intraprostatic steam application as potential focal therapeutic modality for prostatic diseases in a large animal translational model: A feasibility follow-up study.

    Wang-Leandro, Adriano / Willmitzer, Florian / Karol, Agnieszka / Porcellini, Beat / Kronen, Peter / Hiltbrand, Emile M / Rüfenacht, Daniel / Kircher, Patrick R / Richter, Henning

    PloS one

    2019  Volume 14, Issue 12, Page(s) e0226764

    Abstract: Parallel to establishment of diagnostic surveillance protocols for detection of prostatic diseases, novel treatment strategies should be developed. The aim of the present study is to evaluate the feasibility and possible side effects of transrectal, MRI- ... ...

    Abstract Parallel to establishment of diagnostic surveillance protocols for detection of prostatic diseases, novel treatment strategies should be developed. The aim of the present study is to evaluate the feasibility and possible side effects of transrectal, MRI-targeted intraprostatic steam application in dogs as an established large animal translational model for prostatic diseases in humans. Twelve healthy experimental, intact, male beagle dogs without evidence of prostatic pathology were recruited. An initial MRI examination was performed, and MRI-targeted steam was applied intraprostatically immediately thereafter. Serum levels of C-reactive protein (CRP), clinical and ultrasonographic examinations were performed periodically following the procedure to assess treatment effect. Four weeks after treatment, all dogs underwent follow-up MRI examinations and three needle-core biopsies were obtained from each prostatic lobe. Descriptive statistics were performed. MRI-guided intraprostatic steam application was successfully performed in the study population. The first day after steam application, 7/12 dogs had minimal signs of discomfort (grade 1/24 evaluated with the short-form Glasgow Composite Measure Pain Scale) and no dogs showed any sign of discomfort by day 6. CRP elevations were detected in 9/12 dogs during the first week post steam application. Mild to moderate T2 hyperintense intraparenchymal lesions were identified during follow-up MRI in 11/12 dogs four weeks post procedure. Ten of these lesions enhanced mild to moderately after contrast administration. Coagulative necrosis or associated chronic inflammatory response was detected in 80.6% (58/72) of the samples obtained. MRI-targeted intraprostatic steam application is a feasible technique and displays minimal side effects in healthy dogs as translational model for human prostatic diseases. This opens the possibility of minimally invasive novel treatment strategies for intraprostatic lesions.
    MeSH term(s) Ablation Techniques/methods ; Animals ; Dogs ; Feasibility Studies ; Magnetic Resonance Imaging/methods ; Male ; Prostate/diagnostic imaging ; Prostate/surgery ; Prostatic Diseases/diagnostic imaging ; Prostatic Diseases/surgery ; Prostatic Neoplasms/diagnostic imaging ; Prostatic Neoplasms/surgery ; Steam/analysis
    Chemical Substances Steam
    Language English
    Publishing date 2019-12-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0226764
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