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Article: Khronicheskaya yazva zheludka u rebenka, ne diagnostirovannaya pri zhizni.

Nadeev, A P / Karpov, M A / Travin, M A

2023 Volume 85, Issue 3, Page(s) 71–74

Abstract: Chronic gastric ulcer is a rare disease in childhood. The article presents data on the epidemiology and etiology of the disease in children, describes the case of a chronic gastric ulcer complicated by penetration into the liver, perforation, development ...

Title translation	Chronic gastric ulcer in a child not diagnosed during life.
Abstract	Chronic gastric ulcer is a rare disease in childhood. The article presents data on the epidemiology and etiology of the disease in children, describes the case of a chronic gastric ulcer complicated by penetration into the liver, perforation, development of abdominal sepsis, with a fatal outcome in a 7-year-old girl.
MeSH term(s)	Female ; Humans ; Child ; Stomach Ulcer/diagnosis ; Stomach Ulcer/complications ; Liver
Language	Russian
Publishing date	2023-06-05
Publishing country	Russia (Federation)
Document type	Case Reports ; English Abstract ; Journal Article
ZDB-ID	127285-8
ISSN	0004-1955
ISSN	0004-1955
DOI	10.17116/patol20238503171
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Article ; Online: Engineering and geological conditions of dam construction

Balabanov V. / Zhizdyuk A. / Karpov M. / Khudaev I. / Norov B.

E3S Web of Conferences, Vol 401, p

2023 Volume 01049

Abstract: The article presents studies of the engineering-geological conditions of dam construction, consisting of the structure of river valleys and slopes, conditions of rock occurrence, geotechnical and filtration properties of rocks, and geological conditions ... ...

Abstract	The article presents studies of the engineering-geological conditions of dam construction, consisting of the structure of river valleys and slopes, conditions of rock occurrence, geotechnical and filtration properties of rocks, and geological conditions of the dam construction site. A classification of valley types with schemes, classification of rocks about the location of dams, and types of tectonic structure of valleys about the construction of dams are presented. Conclusions are drawn about the most favorable and unfavorable conditions for the construction of dams.
Keywords	Environmental sciences ; GE1-350
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The Study of Efficiency of the Approach to Prevent the Adhesions in the Abdominal Cavity of Rats.

Karpov, M A / Shkurupy, V A / Troitskii, A V

Bulletin of experimental biology and medicine

2021 Volume 171, Issue 4, Page(s) 416–420

Abstract: Adhesions in rat abdominal cavity were studied after laparotomy and subsequent single intraperitoneal injection of 2 ml of 5% aqueous solution of oxidized dextran (OD) with a molecular weight of 40 kDa (oxidation degree 10%). On days 7 and 21 after ... ...

Abstract	Adhesions in rat abdominal cavity were studied after laparotomy and subsequent single intraperitoneal injection of 2 ml of 5% aqueous solution of oxidized dextran (OD) with a molecular weight of 40 kDa (oxidation degree 10%). On days 7 and 21 after laparotomy, the number of adhesions in OD-treated rats was lower by 7.5 and 4 times than in animals not receiving OD. The number of neutrophils in adhesions on day 21 was manyfold lower in OD-treated rats. In 7 and 21 days after laparotomy, the number of fibroblasts in the adhesions of rats receiving and not receiving OD was similar, but 2-fold higher than in the peritoneum of non-operated rats. The content of collagen in adhesions on day 21 after laparotomy in OD-treated rats was 10-fold lower than in animals no receiving OD.
MeSH term(s)	Abdominal Cavity/pathology ; Abdominal Cavity/surgery ; Animals ; Dextrans/administration & dosage ; Dextrans/pharmacology ; Disease Models, Animal ; Injections, Intraperitoneal ; Laparotomy/adverse effects ; Male ; Peritoneum/drug effects ; Peritoneum/pathology ; Postoperative Complications/prevention & control ; Rats ; Rats, Wistar ; Tissue Adhesions/prevention & control ; Treatment Outcome
Chemical Substances	Dextrans
Language	English
Publishing date	2021-09-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	390407-6
ISSN	1573-8221 ; 0007-4888 ; 0365-9615
ISSN (online)	1573-8221
ISSN	0007-4888 ; 0365-9615
DOI	10.1007/s10517-021-05240-1
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Article ; Online: Pregnenolone and progesterone production from natural sterols using recombinant strain of Mycolicibacterium smegmatis mc

Karpov, Mikhail / Strizhov, Nicolai / Novikova, Ludmila / Lobastova, Tatyana / Khomutov, Sergey / Shutov, Andrei / Kazantsev, Alexey / Donova, Marina

Microbial cell factories

2024 Volume 23, Issue 1, Page(s) 105

Abstract: Background: Pregnenolone and progesterone are the life-important steroid hormones regulating essential vital functions in mammals, and widely used in different fields of medicine. Microbiological production of these compounds from sterols is based on ... ...

Abstract	Background: Pregnenolone and progesterone are the life-important steroid hormones regulating essential vital functions in mammals, and widely used in different fields of medicine. Microbiological production of these compounds from sterols is based on the use of recombinant strains expressing the enzyme system cholesterol hydroxylase/C20-C22 lyase (CH/L) of mammalian steroidogenesis. However, the efficiency of the known recombinant strains is still low. New recombinant strains and combination approaches are now needed to produce these steroid hormones. Results: Based on Mycolicibacterium smegmatis, a recombinant strain was created that expresses the steroidogenesis system (CYP11A1, adrenodoxin reductase, adrenodoxin) of the bovine adrenal cortex. The recombinant strain transformed cholesterol and phytosterol to form progesterone among the metabolites. When 3-methoxymethyl ethers of sterols were applied as bioconversion substrates, the corresponding 3-ethers of pregnenolone and dehydroepiandrosterone (DHEA) were identified as major metabolites. Under optimized conditions, the recombinant strain produced 85.2 ± 4.7 mol % 3-methoxymethyl-pregnenolone within 48 h, while production of 3-substituted DHEA was not detected. After the 3-methoxymethyl function was deprotected by acid hydrolysis, crystalline pregnenolone was isolated in high purity (over 98%, w/w). The structures of steroids were confirmed using TLC, HPLC, MS and Conclusion: The use of mycolicybacteria as a microbial platform for the expression of systems at the initial stage of mammalian steroidogenesis ensures the production of valuable steroid hormones-progesterone and pregnenolone from cholesterol. Selective production of pregnenolone from cholesterol is ensured by the use of 3-substituted cholesterol as a substrate and optimization of the conditions for its bioconversion. The results open the prospects for the generation of the new microbial biocatalysts capable of effectively producing value-added steroid hormones.
MeSH term(s)	Cattle ; Animals ; Progesterone ; Pregnenolone/metabolism ; Sterols ; Steroids ; Cholesterol/metabolism ; Cholesterol Side-Chain Cleavage Enzyme/genetics ; Cholesterol Side-Chain Cleavage Enzyme/metabolism ; Phytosterols ; Mammals/metabolism ; Ethers
Chemical Substances	Progesterone (4G7DS2Q64Y) ; Pregnenolone (73R90F7MQ8) ; Sterols ; Steroids ; Cholesterol (97C5T2UQ7J) ; Cholesterol Side-Chain Cleavage Enzyme (EC 1.14.15.6) ; Phytosterols ; Ethers
Language	English
Publishing date	2024-04-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2091377-1
ISSN	1475-2859 ; 1475-2859
ISSN (online)	1475-2859
ISSN	1475-2859
DOI	10.1186/s12934-024-02385-2
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Article ; Online: Structural Changes in the Liver in Post-Toxic Cirrhosis and Its Treatment with Oxidized Dextran. Immunohistochemical Research.

Karpov, M A / Klochin, V D / Nadeev, A P / Shkurupy, V A / Marinkin, I O

Bulletin of experimental biology and medicine

2023 Volume 174, Issue 3, Page(s) 395–398

Abstract: In adult Wistar rats, post-toxic liver cirrhosis was induced by intraperitoneal injection of 50% oil solution of ... ...

Abstract	In adult Wistar rats, post-toxic liver cirrhosis was induced by intraperitoneal injection of 50% oil solution of CCl
MeSH term(s)	Rats ; Animals ; Dextrans/metabolism ; Rats, Wistar ; Liver/metabolism ; Liver Cirrhosis/chemically induced ; Liver Cirrhosis/drug therapy ; Liver Cirrhosis/metabolism ; Collagen/metabolism ; Collagen Type I/genetics ; Collagen Type I/metabolism ; Carbon Tetrachloride/toxicity
Chemical Substances	Dextrans ; Collagen (9007-34-5) ; Collagen Type I ; Carbon Tetrachloride (CL2T97X0V0)
Language	English
Publishing date	2023-02-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	390407-6
ISSN	1573-8221 ; 0007-4888 ; 0365-9615
ISSN (online)	1573-8221
ISSN	0007-4888 ; 0365-9615
DOI	10.1007/s10517-023-05715-3
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Article ; Online: Dual chirped microcomb based parallel ranging at megapixel-line rates.

Lukashchuk, Anton / Riemensberger, Johann / Karpov, Maxim / Liu, Junqiu / Kippenberg, Tobias J

Nature communications

2022 Volume 13, Issue 1, Page(s) 3280

Abstract: Laser-based ranging (LiDAR) - already ubiquitously used in industrial monitoring, atmospheric dynamics, or geodesy - is a key sensor technology. Coherent laser ranging, in contrast to time-of-flight approaches, is immune to ambient light, operates ... ...

Abstract	Laser-based ranging (LiDAR) - already ubiquitously used in industrial monitoring, atmospheric dynamics, or geodesy - is a key sensor technology. Coherent laser ranging, in contrast to time-of-flight approaches, is immune to ambient light, operates continuous-wave allowing higher average powers, and yields simultaneous velocity and distance information. State-of-the-art coherent single laser-detector architectures reach hundreds of kilopixel per second sampling rates, while emerging applications - autonomous driving, robotics, and augmented reality - mandate megapixel per second point sampling to support real-time video-rate imaging. Yet, such rates of coherent LiDAR have not been demonstrated. Recent advances in photonic chip-based microcombs provide a route to higher acquisition speeds via parallelization but require separation of individual channels at the detector side, increasing photonic integration complexity. Here we overcome the challenge and report a hardware-efficient swept dual-soliton microcomb technique that achieves coherent ranging and velocimetry at megapixel per second line scan measurement rates with up to 64 optical channels. Multiheterodyning two synchronously frequency-modulated microcombs yields distance and velocity information of all individual ranging channels on a single receiver alleviating the need for individual separation, detection, and digitization. The reported LiDAR implementation is compatible with photonic integration and demonstrates the significant advantages of acquisition speed afforded by the convergence of optical telecommunication and metrology technologies.
Language	English
Publishing date	2022-06-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-022-30542-x
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Article: Cascade Biotransformation of Phytosterol to Testosterone by Mycolicibacterium neoaurum VKM Ас-1815D and Nocardioides simplex VKM Ас-2033D Strains

Tekucheva, D. N. / Fokina, V. V. / Nikolaeva, V. M. / Shutov, A. A. / Karpov, M. V. / Donova, M. V.

Microbiology. 2022 June, v. 91, no. 3

2022

Abstract: New methods for testosterone production from phytosterol were developed based on its cascade two-stage transformation by actinobacteria Mycolicibacterium neoaurum VKM Ac-1815D and Nocardioides simplex VKM Ac-2033D. Efficient oxidation of the phytosterol ... ...

Abstract	New methods for testosterone production from phytosterol were developed based on its cascade two-stage transformation by actinobacteria Mycolicibacterium neoaurum VKM Ac-1815D and Nocardioides simplex VKM Ac-2033D. Efficient oxidation of the phytosterol side chain by M. neoaurum resulted in formation of the main and side products: androst-4-en-3,20-dione (AD) and androsta-1,4-dien-3,20-dione (ADD), respectively, which were subsequently converted to testosterone by N. simplex. The latter reaction was reversible and catalyzed by the membrane-associated 17β-hydroxysteroid dehydrogenase (17β-HSD) capable of both oxidation and reduction of androstendione at C17. Addition of glucose and limited aeration were found to be the key factors providing for a shift of the 17β-HSD activity towards reduction in whole N. simplex cells. Testosterone production from phytosterol was realized using two approaches: (i) based on M. neoaurum cells inactivation after phytosterol conversion and application of the resting N. simplex biomass for androstenedione reduction and (ii) based on sequential application of the two living cultures. Under optimized conditions, the total yield of testosterone from phytosterol (10 g/L) reached 53 mol %. The results exceeded those reported so far for cascade phytosterol bioconversion to testosterone and may be used as a basis for development of new biotechnologies for production of the valuable steroid compounds, intermediates in the synthesis of modern medical preparations.
Keywords	Pimelobacter simplex ; aeration ; androstenedione ; biomass ; biotransformation ; glucose ; microbiology ; oxidation ; oxidoreductases ; phytosterols ; testosterone
Language	English
Dates of publication	2022-06
Size	p. 303-312.
Publishing place	Pleiades Publishing
Document type	Article
ZDB-ID	209706-0
ISSN	1608-3237 ; 0026-2617
ISSN (online)	1608-3237
ISSN	0026-2617
DOI	10.1134/S0026261722300099
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Abiotic Transformation of Lamotrigine by Redox-Active Mineral and Phenolic Compounds.

Karpov, Marina / Seiwert, Bettina / Mordehay, Vered / Reemtsma, Thorsten / Polubesova, Tamara / Chefetz, Benny

Environmental science & technology

2021 Volume 55, Issue 3, Page(s) 1535–1544

Abstract: The anticonvulsant drug lamotrigine is a recalcitrant environmental pollutant. It was detected in drinking water, surface water, reclaimed wastewater, arable soils, and even in edible crops. In this work, we studied the mechanisms of lamotrigine ... ...

Abstract	The anticonvulsant drug lamotrigine is a recalcitrant environmental pollutant. It was detected in drinking water, surface water, reclaimed wastewater, arable soils, and even in edible crops. In this work, we studied the mechanisms of lamotrigine transformation by a common redox soil mineral, birnessite, in a single-solute system and in bisolute systems with vanillic acid or
MeSH term(s)	Anticonvulsants ; Lamotrigine ; Minerals ; Oxidation-Reduction ; Waste Water ; Water Pollutants, Chemical/analysis
Chemical Substances	Anticonvulsants ; Minerals ; Waste Water ; Water Pollutants, Chemical ; Lamotrigine (U3H27498KS)
Language	English
Publishing date	2021-01-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.0c03631
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Article ; Online: Breathing dissipative solitons in optical microresonators.

Lucas, E / Karpov, M / Guo, H / Gorodetsky, M L / Kippenberg, T J

Nature communications

2017 Volume 8, Issue 1, Page(s) 736

Abstract: Dissipative solitons are self-localised structures resulting from the double balance of dispersion by nonlinearity and dissipation by a driving force arising in numerous systems. In Kerr-nonlinear optical resonators, temporal solitons permit the ... ...

Abstract	Dissipative solitons are self-localised structures resulting from the double balance of dispersion by nonlinearity and dissipation by a driving force arising in numerous systems. In Kerr-nonlinear optical resonators, temporal solitons permit the formation of light pulses in the cavity and the generation of coherent optical frequency combs. Apart from shape-invariant stationary solitons, these systems can support breathing dissipative solitons exhibiting a periodic oscillatory behaviour. Here, we generate and study single and multiple breathing solitons in coherently driven microresonators. We present a deterministic route to induce soliton breathing, allowing a detailed exploration of the breathing dynamics in two microresonator platforms. We measure the relation between the breathing frequency and two control parameters-pump laser power and effective-detuning-and observe transitions to higher periodicity, irregular oscillations and switching, in agreement with numerical predictions. Using a fast detection, we directly observe the spatiotemporal dynamics of individual solitons, which provides evidence of breather synchronisation.Dissipative Kerr solitons enable optical frequency comb generation in microresonators, but these solitons can undergo a breathing transition which impacts the stability of such microcombs. Here, Lucas et al. deterministically induce soliton breathing and directly observe the spatiotemporal dynamics.
Language	English
Publishing date	2017-09-29
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2041-1723
ISSN (online)	2041-1723
DOI	10.1038/s41467-017-00719-w
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Article ; Online: Creation and Functional Analysis of Mycolicibacterium smegmatis Recombinant Strains Carrying the Bacillary Cytochromes CYP106A1 and CYP106A2 Genes

Karpov, M. V. / Nikolaeva, V. M. / Fokina, V. V. / Shutov, A. A. / Kazantsev, A. V. / Strizhov, N. I. / Donova, M. V.

Appl Biochem Microbiol. 2022 Dec., v. 58, no. 9 p.947-957

2022

Abstract: Mycolicibacterium smegmatis mc²155 has been genetically modified to be used as a platform for the expression of heterologous cytochrome P450 monooxygenases by introducing deletions in the kshB and kstD genes that encode key stages of the steroid nucleus ... ...

Abstract	Mycolicibacterium smegmatis mc²155 has been genetically modified to be used as a platform for the expression of heterologous cytochrome P450 monooxygenases by introducing deletions in the kshB and kstD genes that encode key stages of the steroid nucleus enzymatic degradation. Three sets of genetic constructs have been created for heterologous expression of the genes encoding P450 cytochromes CYP106A1 from Bacillus megaterium DSM319 and CYP106A2 from B. megaterium ATCC13368 in M. smegmatis mc²155 (ΔkshBΔkstD) cells. The recombinant plasmids contained monocistronic expression cassettes of cytochrome genes (pNS31 and pNS32), tricistronic cassettes of cytochrome genes together with cDNA copies of adrenodoxin and adrenodoxin reductase genes of the bovine adrenal cortex (pNS33 and pNS34), or monocistronic gene cassettes of chimeric cytochromes fused with the DNA sequence encoding the CYP116B2 reductase domain from the soil bacterium Rhodococcus sp. NCIMB 9784 (pNS35 and pNS36). The recombinant strains of mycolicibacteria were shown to selectively monohydroxylate androstenedione (AD) under growth conditions. The product was identified as 15β-hydroxyandrostenedione (15β-OH-AD) by mass spectrometry and ¹H and ¹³C NMR spectroscopy. The maximum level of 15β-OH-AD production (17.3 ± 1.5 mg/L) was observed when using the recombinant M. smegmatis mc²155 (ΔkshBΔkstD) (pNS32) strain, which expresses a single cyp106A2 gene from B. megaterium ATCC13368. Host proteins of M. smegmatis mc²155 were shown to be capable of supplying electrons to heterologous cytochromes to support their hydroxylation activity. The results we obtained are of a priority character, expand the understanding of the hydroxylation of steroid compounds by bacterial cytochromes CYP106A1/A2, and are important for the creation of microbial strains for producing valuable hydroxysteroids.
Keywords	Bacillus megaterium ; adrenal cortex ; androstenedione ; cattle ; cytochrome P-450 ; ferredoxin-NADP reductase ; genes ; heterologous gene expression ; hydroxylation ; mass spectrometry ; nuclear magnetic resonance spectroscopy ; nucleotide sequences ; plasmids ; soil bacteria
Language	English
Dates of publication	2022-12
Size	p. 947-957.
Publishing place	Pleiades Publishing
Document type	Article ; Online
ZDB-ID	412550-2
ISSN	1608-3024 ; 0003-6838
ISSN (online)	1608-3024
ISSN	0003-6838
DOI	10.1134/S0003683822090058
Database	NAL-Catalogue (AGRICOLA)

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