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  1. AU="Kaspar, Charles W"
  2. AU="Wallach, E E"
  3. AU="Temnikov, P"
  4. AU="Gomez-Verjan, Juan Carlos"
  5. AU="Mayle, Francis E."
  6. AU="Rhoades, Elizabeth"
  7. AU="Riaz, Huma"
  8. AU="Eliseu, Gabriel"
  9. AU="Hill, Lori R"
  10. AU="Boppana, Suresh B"

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  1. Artikel ; Online: Escherichia coli O157: H7 Acid Tolerance and Survival in Apple Cider.

    Miller, Leslie Garland / Kaspar, Charles W

    Journal of food protection

    2019  Band 57, Heft 6, Seite(n) 460–464

    Abstract: The survival of two Escherichia coli O157:H7 (ATCC 43889 and 43895) and a control strain E. coli was compared in apple cider and in Trypticase soy broth (TSB) adjusted to low and high pH. The O157:H7 strains were detectable in apple cider after 14 to 21 ... ...

    Abstract The survival of two Escherichia coli O157:H7 (ATCC 43889 and 43895) and a control strain E. coli was compared in apple cider and in Trypticase soy broth (TSB) adjusted to low and high pH. The O157:H7 strains were detectable in apple cider after 14 to 21 days at 4°C, whereas the control strain could not be detected (> 4-log reduction) after 5 to 7 days. During the first 14 days of storage at 4°C, the levels of strain 43889 decreased by ~3 logs, whereas levels of strain 43895 were unchanged. Survival of O157:H7 strains and the control strain were unaffected by the presence of potassium sorbate or sodium benzoate, except in one instance. Sodium benzoate caused a decrease of 57% in strain 43895 after 21 days, but ~10
    Sprache Englisch
    Erscheinungsdatum 2019-06-06
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 243284-5
    ISSN 1944-9097 ; 0362-028X
    ISSN (online) 1944-9097
    ISSN 0362-028X
    DOI 10.4315/0362-028X-57.6.460
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Decontamination of Plastic and Wooden Cutting Boards for Kitchen Use.

    Ak, Nese O / Cliver, Dean O / Kaspar, Charles W

    Journal of food protection

    2019  Band 57, Heft 1, Seite(n) 23–30

    Abstract: Decontamination of Plastic and wooden cutting boards was studied, with a view to preventing cross-contamination of foods in home kitchens. New and used Plastic (four polymers plus hard rubber) and wood (nine hardwoods) boards were cut into 5-cm square ... ...

    Abstract Decontamination of Plastic and wooden cutting boards was studied, with a view to preventing cross-contamination of foods in home kitchens. New and used Plastic (four polymers plus hard rubber) and wood (nine hardwoods) boards were cut into 5-cm square blocks (25 cm
    Sprache Englisch
    Erscheinungsdatum 2019-06-10
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 243284-5
    ISSN 1944-9097 ; 0362-028X
    ISSN (online) 1944-9097
    ISSN 0362-028X
    DOI 10.4315/0362-028X-57.1.23
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Cutting Boards of Plastic and Wood Contaminated Experimentally with Bacteria.

    Ak, Nese O / Cliver, Dean O / Kaspar, Charles W

    Journal of food protection

    2019  Band 57, Heft 1, Seite(n) 16–22

    Abstract: The microbiology of Plastic and wooden cutting boards was studied, regarding cross-contamination of foods in home kitchens. New and used Plastic (four polymers plus hard rubber) and wood (nine hardwoods) cutting boards were cut into 5-cm squares ("blocks" ...

    Abstract The microbiology of Plastic and wooden cutting boards was studied, regarding cross-contamination of foods in home kitchens. New and used Plastic (four polymers plus hard rubber) and wood (nine hardwoods) cutting boards were cut into 5-cm squares ("blocks"). Escherichia coli (two nonpathogenic strains plus type O157:H7), Listeria innocua , L. monocytogenes , or Salmonella typhimurium was applied to the 25-cm
    Sprache Englisch
    Erscheinungsdatum 2019-06-06
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 243284-5
    ISSN 1944-9097 ; 0362-028X
    ISSN (online) 1944-9097
    ISSN 0362-028X
    DOI 10.4315/0362-028X-57.1.16
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Chronological set of E. coli O157:H7 bovine strains establishes a role for repeat sequences and mobile genetic elements in genome diversification.

    Stanton, Eliot / Wahlig, Taylor A / Park, Dongjin / Kaspar, Charles W

    BMC genomics

    2020  Band 21, Heft 1, Seite(n) 562

    Abstract: Background: Enterohemorrhagic Escherichia coli O157:H7 (EHEC) is a significant foodborne pathogen that resides asymptomatically within cattle and other ruminants. The EHEC genome harbors an extensive collection of mobile genetic elements (MGE), ... ...

    Abstract Background: Enterohemorrhagic Escherichia coli O157:H7 (EHEC) is a significant foodborne pathogen that resides asymptomatically within cattle and other ruminants. The EHEC genome harbors an extensive collection of mobile genetic elements (MGE), including multiple prophage, prophage-like elements, plasmids, and insertion sequence (IS) elements.
    Results: A chronological collection of EHEC strains (FRIK804, FRIK1275, and FRIK1625) isolated from a Wisconsin dairy farm (farm X) comprised a closely related clade genetically differentiated by structural alterations to the chromosome. Comparison of the FRIK804 genome with a reference EHEC strain Sakai found a unique prophage like element (PLE, indel 1) and an inversion (1.15 Mb) situated symmetrically with respect to the terminus region. Detailed analysis determined the inversion was due to homologous recombination between repeat sequences in prophage. The three farm X strains were distinguished by the presence or absence of indel 3 (61 kbp) and indel 4 (48 kbp); FRIK804 contained both of these regions, FRIK1275 lacked indel 4, and indels 3 and 4 were both absent in FRIK1625. Indel 3 was the stx2 prophage and indel 4 involved a deletion between two adjacent prophage with shared repeat sequences. Both FRIK804 and FRIK1275 produced functional phage while FRIK1625 did not, which is consistent with indel 3. Due to their involvement in recombination events, direct and inverted repeat sequences were identified, and their locations mapped to the chromosome. FRIK804 had a greater number and overall length of repeat sequences than E. coli K12 strain MG1655. Repeat sequences were most commonly associated with MGE.
    Conclusions: This research demonstrated that three EHEC strains from a Wisconsin dairy farm were closely related and distinguished by variability within prophage regions and other MGE. Chromosome alterations were associated with recombination events between repeat sequences. An inventory of direct and inverted repeat sequences found a greater abundance and total length of repeat sequences in the EHEC strains compared to E. coli strain MG1655. The locations of the repeat sequences were biased towards MGE. The findings from this study expand our understanding of the precise molecular events and elements that contributed to genetic diversification of wild-type EHEC in the bovine and farm environments.
    Mesh-Begriff(e) Animals ; Bacteriophages/genetics ; Cattle ; DNA Transposable Elements ; Escherichia coli Infections ; Escherichia coli O157/genetics ; Prophages/genetics
    Chemische Substanzen DNA Transposable Elements
    Sprache Englisch
    Erscheinungsdatum 2020-08-17
    Erscheinungsland England
    Dokumenttyp Journal Article
    ISSN 1471-2164
    ISSN (online) 1471-2164
    DOI 10.1186/s12864-020-06943-x
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Genome Sequences of 14 Escherichia coli O157:H7 Strains Isolated before and during the Time Frame of the 2018 Multistate Outbreak Associated with Romaine Lettuce.

    Stanton, Eliot / Wagner, Sara / Florek, Kelsey R / Kaspar, Charles W

    Microbiology resource announcements

    2020  Band 9, Heft 29

    Abstract: Several outbreaks ... ...

    Abstract Several outbreaks of
    Sprache Englisch
    Erscheinungsdatum 2020-07-16
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.00458-20
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: A Single Nucleotide Polymorphism in

    Wahlig, Taylor A / Stanton, Eliot / Godfrey, Jared J / Stasic, Andrew J / Wong, Amy C L / Kaspar, Charles W

    Frontiers in microbiology

    2021  Band 12, Seite(n) 671453

    Abstract: The outer membrane ... ...

    Abstract The outer membrane of
    Sprache Englisch
    Erscheinungsdatum 2021-06-02
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2021.671453
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Genome Sequence of the Extremely Acidophilic Fungus Acidomyces richmondensis FRIK2901.

    Rosienski, Matthew D / Lee, Mi-Kyung / Yu, Jae-Hyuk / Kaspar, Charles W / Gibbons, John G

    Microbiology resource announcements

    2018  Band 7, Heft 16

    Abstract: Acidomyces richmondensis is an extremophilic fungal species found in warm, acidic, and metal-rich environments. To improve upon the existing reference genome, we used PacBio and Illumina sequencing to assemble a highly contiguous 29.3-Mb genome of A. ... ...

    Abstract Acidomyces richmondensis is an extremophilic fungal species found in warm, acidic, and metal-rich environments. To improve upon the existing reference genome, we used PacBio and Illumina sequencing to assemble a highly contiguous 29.3-Mb genome of A. richmondensis FRIK2901.
    Sprache Englisch
    Erscheinungsdatum 2018-10-25
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.01314-18
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: Phosphate and carbohydrate facilitate the formation of filamentous Salmonella enterica during osmotic stress.

    Lensmire, Joshua M / Pratt, Zachary L / Wong, Amy C L / Kaspar, Charles W

    Microbiology (Reading, England)

    2018  Band 164, Heft 12, Seite(n) 1503–1513

    Abstract: Salmonella enterica is a human pathogen that can produce filamentous cells in response to environmental stress. The molecular mediators and biosynthetic pathways that contribute to the formation of filamentous cells (>10 µm in length) during osmotic ... ...

    Abstract Salmonella enterica is a human pathogen that can produce filamentous cells in response to environmental stress. The molecular mediators and biosynthetic pathways that contribute to the formation of filamentous cells (>10 µm in length) during osmotic stress are mostly unknown. The comparison of filamentous and non-filamentous cells in this study was aided by the use of a filtration step to separate cell types. Osmotic stress caused an efflux of phosphate from cells, and the addition of phosphate and a carbohydrate to Luria broth with 7 % NaCl (LB-7NaCl) significantly increased the proportion of filamentous cells in the population (58 %). In addition to direct measurements of intracellular and extracellular phosphate concentrations, the relative abundance of the iraP transcript that is induced by phosphate limitation was monitored. Non-filamentous cells had a greater relative abundance of iraP transcript than filamentous cells. IraP also affects the stability of RpoS, which regulates the general stress regulon, and was detected in non-filamentous cells but not filamentous cells. Markers of metabolic pathways for the production of acetyl-CoA (pflB, encoding for pyruvate formate lyase) and fatty acids (fabH) that are essential to membrane biosynthesis were found in greater abundance in filamentous cells than non-filamentous cells. There were no differences in the DNA, protein and biomass levels in filamentous and non-filamentous cells after 48 h of incubation, although the filamentous cells produced significantly (P<0.05) more acetate. This study found that phosphate and carbohydrate enhanced the formation of filamentous cells during osmotic stress, and there were differences in key regulatory elements and markers of metabolic pathways in filamentous and non-filamentous S. enterica.
    Mesh-Begriff(e) Acetates/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biological Transport ; Carbohydrate Metabolism ; Gene Expression Regulation, Bacterial ; Osmoregulation/genetics ; Osmotic Pressure ; Phosphates/metabolism ; Salmonella enterica/cytology ; Salmonella enterica/metabolism ; Salmonella enterica/physiology ; Sodium Chloride/metabolism
    Chemische Substanzen Acetates ; Bacterial Proteins ; Phosphates ; Sodium Chloride (451W47IQ8X)
    Sprache Englisch
    Erscheinungsdatum 2018-10-16
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1180712-x
    ISSN 1465-2080 ; 1350-0872
    ISSN (online) 1465-2080
    ISSN 1350-0872
    DOI 10.1099/mic.0.000731
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel ; Online: Validation of Pepperoni Processes for Control of Escherichia coli O157:H7

    Hinkens, Jay C / Faith, Nancy G / Lorang, Timothy D / Bailey, Phillip / Buege, Dennis / Kaspar, Charles W / Luchansky, John B

    Journal of food protection

    2019  Band 59, Heft 12, Seite(n) 1260–1266

    Abstract: The outbreak of Escherichia coli O157:H7 linked with dry-cured salami in late 1994 prompted regulatory action that required manufacturers of fermented products to demonstrate a 5-log unit reduction in counts of this pathogen during processing. Therefore, ...

    Abstract The outbreak of Escherichia coli O157:H7 linked with dry-cured salami in late 1994 prompted regulatory action that required manufacturers of fermented products to demonstrate a 5-log unit reduction in counts of this pathogen during processing. Therefore, pepperoni batter (75% pork:25% beef with a fat content of ca. 32%) was inoculated with a pediococcal starter culture and a five-strain mixture of E. coli O157:H7 (≥2 × 10
    Sprache Englisch
    Erscheinungsdatum 2019-06-07
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 243284-5
    ISSN 1944-9097 ; 0362-028X
    ISSN (online) 1944-9097
    ISSN 0362-028X
    DOI 10.4315/0362-028X-59.12.1260
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel: 16 Methods for Detecting Microbial Pathogens in Food and Water.

    Kaspar, Charles W / Tartera, Carmen

    Methods in microbiology

    2008  Band 22, Seite(n) 497–531

    Abstract: Newly developed methods for the detection of bacteria and viruses have provided microbiologists with the means to rapidly identify and monitor specific microorganisms in food and water. Traditional methods of testing involve culture techniques to ... ...

    Abstract Newly developed methods for the detection of bacteria and viruses have provided microbiologists with the means to rapidly identify and monitor specific microorganisms in food and water. Traditional methods of testing involve culture techniques to increase the numbers of the organism to a detectable level, followed by isolation and biochemical identification. This chapter focuses on the methodologies to detect pathogens and indicator organisms; however, the methods described are applicable to most bacteria. As detection and isolation methods have improved, a growing number of pathogens have been identified as important food- and waterborne pathogens. This chapter describes the use of nucleic acid and antibody probes that have the potential to circumvent the need to culture the organism prior to identification. Nucleic acid probes have become a valuable diagnostic reagent in the identification of human and animal pathogens and have made possible the identification of viruses and bacteria that are difficult, if not impossible, to cultivate. DNA probes have also proved to be a useful tool for identifying and monitoring the organisms in food and the environment.
    Sprache Englisch
    Erscheinungsdatum 2008-04-28
    Erscheinungsland England
    Dokumenttyp Journal Article
    ISSN 0580-9517
    ISSN 0580-9517
    DOI 10.1016/S0580-9517(08)70253-8
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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