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  1. AU="Kasthuri, Thirupathi"
  2. AU="Pirtskhalava, Tamar"
  3. AU="Saridakis, E N"
  4. AU="Vithana, Eranga N"
  5. AU="Suárez-Lledó, M"
  6. AU="Olivo-Marston, Susan"
  7. AU="Denise P Momesso"
  8. AU="Obrecht-Sturm, Denise"

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  1. Artikel ; Online: Rapid-killing efficacy substantiates the antiseptic property of the synergistic combination of carvacrol and nerol against nosocomial pathogens.

    Kasthuri, Thirupathi / Swetha, Thirukannamangai Krishnan / Bhaskar, James Prabhanand / Pandian, Shunmugiah Karutha

    Archives of microbiology

    2022  Band 204, Heft 9, Seite(n) 590

    Abstract: Globally, new classes of synthetic and natural antibiotics and antivirulents have continuously been validated for their potential broad-spectrum antagonistic activity with the aim of identifying an effective active molecule to prevent the spread of ... ...

    Abstract Globally, new classes of synthetic and natural antibiotics and antivirulents have continuously been validated for their potential broad-spectrum antagonistic activity with the aim of identifying an effective active molecule to prevent the spread of infectious agents in both food industry and medical field. In view of this, present study is aimed at evaluating the rapid killing efficacy of bioactive molecules Carvacrol (C) and Nerol (N) through British Standard European Norm 1276: phase2/step1 (EN1276) protocol. Active molecules C and N showed broad-spectrum antimicrobial activity against the test strains Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus hirae at concentration range of 78.125, 625, 156.25 and 312.5 μg/mL, respectively, for C, and 625 μg/mL for N. Whereas, combinatorial approach showed efficient activity with four times reduced concentration of C and N at 78.125 and 156.25 µg/mL, respectively, against test strains. Further, EN1276 results proved the rapid killing efficacy of test strains in 1 min of contact time with significant (> 5 log) growth reduction at 100X concentration of actives. SEM analysis and reduced concentration of protease, lipids and carbohydrate contents of treated group biofilm components ascertained preformed biofilm disruption potential of C + N on polystyrene and nail surfaces. C + N at synergistic concentration exhibited no adverse effect on HaCaT cells at 78.125 µg/mL (C) + 156.25 µg/mL (N). Taken together, based on the observed experimental results, present study evidence the antiseptic/disinfectant ability of C + N and suggest that the combination can preferentially be used in foam-based hand wash formulations.
    Mesh-Begriff(e) Acyclic Monoterpenes ; Anti-Bacterial Agents/pharmacology ; Anti-Infective Agents, Local/pharmacology ; Cross Infection/prevention & control ; Cymenes ; Escherichia coli ; Humans ; Microbial Sensitivity Tests ; Pseudomonas aeruginosa ; Staphylococcus aureus
    Chemische Substanzen Acyclic Monoterpenes ; Anti-Bacterial Agents ; Anti-Infective Agents, Local ; Cymenes ; carvacrol (9B1J4V995Q) ; geraniol (L837108USY)
    Sprache Englisch
    Erscheinungsdatum 2022-09-02
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 124824-8
    ISSN 1432-072X ; 0302-8933
    ISSN (online) 1432-072X
    ISSN 0302-8933
    DOI 10.1007/s00203-022-03197-x
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  2. Artikel: Rapid-killing efficacy substantiates the antiseptic property of the synergistic combination of carvacrol and nerol against nosocomial pathogens

    Kasthuri, Thirupathi / Swetha, Thirukannamangai Krishnan / Bhaskar, James Prabhanand / Pandian, Shunmugiah Karutha

    Archives of microbiology. 2022 Sept., v. 204, no. 9

    2022  

    Abstract: Globally, new classes of synthetic and natural antibiotics and antivirulents have continuously been validated for their potential broad-spectrum antagonistic activity with the aim of identifying an effective active molecule to prevent the spread of ... ...

    Abstract Globally, new classes of synthetic and natural antibiotics and antivirulents have continuously been validated for their potential broad-spectrum antagonistic activity with the aim of identifying an effective active molecule to prevent the spread of infectious agents in both food industry and medical field. In view of this, present study is aimed at evaluating the rapid killing efficacy of bioactive molecules Carvacrol (C) and Nerol (N) through British Standard European Norm 1276: phase2/step1 (EN1276) protocol. Active molecules C and N showed broad-spectrum antimicrobial activity against the test strains Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus hirae at concentration range of 78.125, 625, 156.25 and 312.5 μg/mL, respectively, for C, and 625 μg/mL for N. Whereas, combinatorial approach showed efficient activity with four times reduced concentration of C and N at 78.125 and 156.25 µg/mL, respectively, against test strains. Further, EN1276 results proved the rapid killing efficacy of test strains in 1 min of contact time with significant (> 5 log) growth reduction at 100X concentration of actives. SEM analysis and reduced concentration of protease, lipids and carbohydrate contents of treated group biofilm components ascertained preformed biofilm disruption potential of C + N on polystyrene and nail surfaces. C + N at synergistic concentration exhibited no adverse effect on HaCaT cells at 78.125 µg/mL (C) + 156.25 µg/mL (N). Taken together, based on the observed experimental results, present study evidence the antiseptic/disinfectant ability of C + N and suggest that the combination can preferentially be used in foam-based hand wash formulations.
    Schlagwörter Enterococcus hirae ; Escherichia coli ; Pseudomonas aeruginosa ; Staphylococcus aureus ; adverse effects ; antimicrobial properties ; antiseptics ; biofilm ; carbohydrates ; carvacrol ; cross infection ; disinfectants ; food industry ; growth retardation ; microbiology ; nerol ; polystyrenes ; proteinases
    Sprache Englisch
    Erscheinungsverlauf 2022-09
    Umfang p. 590.
    Erscheinungsort Springer Berlin Heidelberg
    Dokumenttyp Artikel
    ZDB-ID 124824-8
    ISSN 1432-072X ; 0302-8933
    ISSN (online) 1432-072X
    ISSN 0302-8933
    DOI 10.1007/s00203-022-03197-x
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  3. Artikel ; Online: Two novel phages PSPa and APPa inhibit planktonic, sessile and persister populations of Pseudomonas aeruginosa, and mitigate its virulence in Zebrafish model.

    Karthika, Chandrasekar / Malligarjunan, Nambiraman / Jothi, Ravi / Kasthuri, Thirupathi / Alexpandi, Rajaiah / Ravi, Arumugam Veera / Pandian, Shunmugiah Karutha / Gowrishankar, Shanmugaraj

    Scientific reports

    2023  Band 13, Heft 1, Seite(n) 19033

    Abstract: The present study explores the avenue of phage therapy as an alternative antimicrobial therapeutic approach to counter multidrug-resistant (MDR) Pseudomonas aeruginosa infection. Our study investigated two novel virulent phages PSPa and APPa, specific to ...

    Abstract The present study explores the avenue of phage therapy as an alternative antimicrobial therapeutic approach to counter multidrug-resistant (MDR) Pseudomonas aeruginosa infection. Our study investigated two novel virulent phages PSPa and APPa, specific to P. aeruginosa, in which in vitro evaluations were carried out to assess the therapeutic potential of phages. Both the identified phages exhibited host specificity by showing antagonistic activity of about 96.43% (27/28) and 92.85% (26/28) towards the 28 MDR clinical isolates of P. aeruginosa. The PSPa phage was found to have linear dsDNA with a sequence length of 66,368 bp and 92 ORFs, of which 32 were encoded for known functions of the phage life cycle and the remaining 60 were hypothetical functions. The APPa phage was found to have linear dsDNA with 59,591 bp of genome length and 79 ORFs, of which 15 were found to have known phage functions and the remaining 64 were found to be hypothetical proteins. Notably, the genome of both the phages lacks genes coding for tRNA, rRNA, and tmRNA. The phylogenetic analysis revealed that PSPa and APPa share > 95% sequence similarity with previously sequenced Pseudomonas viruses of their respective families. Further, the in vivo efficacy evaluation using the zebrafish model revealed that the treatment with PSPa and APPa has remarkably improved the survival rate of bacterial-infected zebrafish, reinforcing the anti-infective potential of the isolated phages PSPa and APPa against P. aeruginosa infection.
    Mesh-Begriff(e) Humans ; Animals ; Bacteriophages ; Pseudomonas aeruginosa/genetics ; Zebrafish ; Virulence ; Phylogeny ; Plankton ; Pseudomonas Phages
    Chemische Substanzen diadenosine pyrophosphate (14938-28-4)
    Sprache Englisch
    Erscheinungsdatum 2023-11-03
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-45313-x
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: 5-hydroxymethyl-2-furaldehyde impairs Candida albicans - Staphylococcus epidermidis interaction in co-culture by suppressing crucial supportive virulence traits

    Swetha, Thirukannamangai Krishnan / Subramenium, Ganapathy Ashwinkumar / Kasthuri, Thirupathi / Sharumathi, Rajendran / Pandian, Shunmugiah Karutha

    Microbial pathogenesis. 2021 Sept., v. 158

    2021  

    Abstract: Polymicrobial biofilms involving fungal-bacterial interactions are stated to modulate host immune response and exhibit enhanced antibiotic resistance. In this milieu, clinically important opportunistic pathogens Candida albicans and Staphylococcus ... ...

    Abstract Polymicrobial biofilms involving fungal-bacterial interactions are stated to modulate host immune response and exhibit enhanced antibiotic resistance. In this milieu, clinically important opportunistic pathogens Candida albicans and Staphylococcus epidermidis associate synergistically and instigate implant and blood stream infections. Impediment of virulence traits that support successive pathogenic lifestyle and inter-kingdom interactions without altering the microbial growth represents an attractive alternate strategy. To accomplish this objective, 5-hydroxymethyl-2-furaldehyde (5HM2F), a reported antibiofilm agent against C. albicans, was considered for this study. 5HM2F significantly repressed the biofilm formation of S. epidermidis and mixed-species at 300 μg/mL and 400 μg/mL, respectively without modulating the growth. Microscopic analyses and phenotypic assays explicated the competency of 5HM2F to impede biofilm formation, hyphal growth, initial attachment, intercellular adhesion, and fungal-bacterial interaction. Further, 5HM2F greatly reduced the secreted hydrolases production. Reduced content of biofilm matrix components upon 5HM2F treatment was believed to be the underlying reason for enhanced antibiotic and/antifungal susceptibility. Additionally, qPCR analysis correlated well with in vitro bioassays wherein, 5HM2F was identified to repress the expression of important genes associated with hyphal morphogenesis, adhesion, biofilm formation and virulence in both mono-species and mixed-species. Reduced virulence and colonization of mono-species and mixed-species in 5HM2F treated Caenorhabditis elegans substantiated the antibiofilm and antivirulence potential of 5HM2F. Overall, this study proposes 5HM2F as a potent therapeutic candidate against single and mixed-species biofilm infections of C. albicans and S. epidermidis.
    Schlagwörter Caenorhabditis elegans ; Candida albicans ; Staphylococcus epidermidis ; adhesion ; antibiotic resistance ; antibiotics ; biofilm ; blood flow ; cell adhesion ; coculture ; hydrolases ; hydroxymethylfurfural ; hyphae ; immune response ; microbial growth ; morphogenesis ; pathogenesis ; phenotype ; therapeutics ; virulence
    Sprache Englisch
    Erscheinungsverlauf 2021-09
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel
    Anmerkung NAL-AP-2-clean
    ZDB-ID 632772-2
    ISSN 1096-1208 ; 0882-4010
    ISSN (online) 1096-1208
    ISSN 0882-4010
    DOI 10.1016/j.micpath.2021.104990
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  5. Artikel ; Online: 5-hydroxymethyl-2-furaldehyde impairs Candida albicans - Staphylococcus epidermidis interaction in co-culture by suppressing crucial supportive virulence traits.

    Swetha, Thirukannamangai Krishnan / Subramenium, Ganapathy Ashwinkumar / Kasthuri, Thirupathi / Sharumathi, Rajendran / Pandian, Shunmugiah Karutha

    Microbial pathogenesis

    2021  Band 158, Seite(n) 104990

    Abstract: Polymicrobial biofilms involving fungal-bacterial interactions are stated to modulate host immune response and exhibit enhanced antibiotic resistance. In this milieu, clinically important opportunistic pathogens Candida albicans and Staphylococcus ... ...

    Abstract Polymicrobial biofilms involving fungal-bacterial interactions are stated to modulate host immune response and exhibit enhanced antibiotic resistance. In this milieu, clinically important opportunistic pathogens Candida albicans and Staphylococcus epidermidis associate synergistically and instigate implant and blood stream infections. Impediment of virulence traits that support successive pathogenic lifestyle and inter-kingdom interactions without altering the microbial growth represents an attractive alternate strategy. To accomplish this objective, 5-hydroxymethyl-2-furaldehyde (5HM2F), a reported antibiofilm agent against C. albicans, was considered for this study. 5HM2F significantly repressed the biofilm formation of S. epidermidis and mixed-species at 300 μg/mL and 400 μg/mL, respectively without modulating the growth. Microscopic analyses and phenotypic assays explicated the competency of 5HM2F to impede biofilm formation, hyphal growth, initial attachment, intercellular adhesion, and fungal-bacterial interaction. Further, 5HM2F greatly reduced the secreted hydrolases production. Reduced content of biofilm matrix components upon 5HM2F treatment was believed to be the underlying reason for enhanced antibiotic and/antifungal susceptibility. Additionally, qPCR analysis correlated well with in vitro bioassays wherein, 5HM2F was identified to repress the expression of important genes associated with hyphal morphogenesis, adhesion, biofilm formation and virulence in both mono-species and mixed-species. Reduced virulence and colonization of mono-species and mixed-species in 5HM2F treated Caenorhabditis elegans substantiated the antibiofilm and antivirulence potential of 5HM2F. Overall, this study proposes 5HM2F as a potent therapeutic candidate against single and mixed-species biofilm infections of C. albicans and S. epidermidis.
    Mesh-Begriff(e) Biofilms ; Candida albicans ; Coculture Techniques ; Furaldehyde/analogs & derivatives ; Staphylococcus epidermidis ; Virulence
    Chemische Substanzen 5-hydroxymethylfurfural (70ETD81LF0) ; Furaldehyde (DJ1HGI319P)
    Sprache Englisch
    Erscheinungsdatum 2021-05-25
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 632772-2
    ISSN 1096-1208 ; 0882-4010
    ISSN (online) 1096-1208
    ISSN 0882-4010
    DOI 10.1016/j.micpath.2021.104990
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: Suppression of Thiol-Dependent Antioxidant System and Stress Response in Methicillin-Resistant Staphylococcus aureus by Docosanol: Explication Through Proteome Investigation.

    Lakshmi, Selvaraj Alagu / Prasath, Krishnan Ganesh / Tamilmuhilan, Kannapiran / Srivathsan, Adimoolam / Shafreen, Raja Mohamed Beema / Kasthuri, Thirupathi / Pandian, Shunmugiah Karutha

    Molecular biotechnology

    2022  Band 64, Heft 5, Seite(n) 575–589

    Abstract: The present study was aimed to investigate the effect of docosanol on the protein expression profile of methicillin-resistant Staphylococcus aureus (MRSA). Thus, two-dimensional gel electrophoresis coupled with MALDI-TOF MS technique was utilized to ... ...

    Abstract The present study was aimed to investigate the effect of docosanol on the protein expression profile of methicillin-resistant Staphylococcus aureus (MRSA). Thus, two-dimensional gel electrophoresis coupled with MALDI-TOF MS technique was utilized to identify the differentially regulated proteins in the presence of docosanol. A total of 947 protein spots were identified from the intracellular proteome of both control and docosanol treated samples among which 40 spots were differentially regulated with a fold change greater than 1.0. Prominently, the thiol-dependent antioxidant system and stress response proteins are downregulated in MRSA, which are critical for survival during oxidative stress. In particular, docosanol downregulated the expression of Tpx, AhpC, BshC, BrxA, and YceI with a fold change of 1.4 (p = 0.02), 1.4 (p = 0.01), 1.6 (p = 0.002), 4.9 (p = 0.02), and 1.4 (p = 0.02), respectively. In addition, docosanol reduced the expression of proteins involved in purine metabolic pathways, biofilm growth cycle, and virulence factor production. Altogether, these findings suggest that docosanol could efficiently target the antioxidant pathway by reducing the expression of bacillithiol and stress-associated proteins.
    Mesh-Begriff(e) Anti-Bacterial Agents/pharmacology ; Antioxidants/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Fatty Alcohols ; Methicillin-Resistant Staphylococcus aureus/genetics ; Microbial Sensitivity Tests ; Proteome/genetics ; Proteome/metabolism ; Sulfhydryl Compounds/metabolism
    Chemische Substanzen Anti-Bacterial Agents ; Antioxidants ; Bacterial Proteins ; Fatty Alcohols ; Proteome ; Sulfhydryl Compounds ; docosanol (9G1OE216XY)
    Sprache Englisch
    Erscheinungsdatum 2022-01-12
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 1193057-3
    ISSN 1559-0305 ; 1073-6085
    ISSN (online) 1559-0305
    ISSN 1073-6085
    DOI 10.1007/s12033-021-00434-4
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4031: Hefte anzeigen Standort:
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  7. Artikel: Suppression of Thiol-Dependent Antioxidant System and Stress Response in Methicillin-Resistant Staphylococcus aureus by Docosanol: Explication Through Proteome Investigation

    Lakshmi, Selvaraj Alagu / Prasath, Krishnan Ganesh / Tamilmuhilan, Kannapiran / Srivathsan, Adimoolam / Shafreen, Raja Mohamed Beema / Kasthuri, Thirupathi / Pandian, Shunmugiah Karutha

    Molecular biotechnology. 2022 May, v. 64, no. 5

    2022  

    Abstract: The present study was aimed to investigate the effect of docosanol on the protein expression profile of methicillin-resistant Staphylococcus aureus (MRSA). Thus, two-dimensional gel electrophoresis coupled with MALDI-TOF MS technique was utilized to ... ...

    Abstract The present study was aimed to investigate the effect of docosanol on the protein expression profile of methicillin-resistant Staphylococcus aureus (MRSA). Thus, two-dimensional gel electrophoresis coupled with MALDI-TOF MS technique was utilized to identify the differentially regulated proteins in the presence of docosanol. A total of 947 protein spots were identified from the intracellular proteome of both control and docosanol treated samples among which 40 spots were differentially regulated with a fold change greater than 1.0. Prominently, the thiol-dependent antioxidant system and stress response proteins are downregulated in MRSA, which are critical for survival during oxidative stress. In particular, docosanol downregulated the expression of Tpx, AhpC, BshC, BrxA, and YceI with a fold change of 1.4 (p = 0.02), 1.4 (p = 0.01), 1.6 (p = 0.002), 4.9 (p = 0.02), and 1.4 (p = 0.02), respectively. In addition, docosanol reduced the expression of proteins involved in purine metabolic pathways, biofilm growth cycle, and virulence factor production. Altogether, these findings suggest that docosanol could efficiently target the antioxidant pathway by reducing the expression of bacillithiol and stress-associated proteins.
    Schlagwörter antioxidants ; biofilm ; biotechnology ; methicillin-resistant Staphylococcus aureus ; oxidative stress ; protein synthesis ; proteome ; stress response ; two-dimensional gel electrophoresis ; virulence
    Sprache Englisch
    Erscheinungsverlauf 2022-05
    Umfang p. 575-589.
    Erscheinungsort Springer US
    Dokumenttyp Artikel
    ZDB-ID 1193057-3
    ISSN 1559-0305 ; 1073-6085
    ISSN (online) 1559-0305
    ISSN 1073-6085
    DOI 10.1007/s12033-021-00434-4
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  8. Artikel: Evaluation of antibiofilm potential of four-domain α-amylase from Streptomyces griseus against exopolysaccharides (EPS) of bacterial pathogens using Danio rerio

    Lakshmi, Selvaraj Alagu / Alexpandi, Rajaiah / Shafreen, Raja Mohamed Beema / Tamilmuhilan, Kannapiran / Srivathsan, Adimoolam / Kasthuri, Thirupathi / Ravi, Arumugam Veera / Shiburaj, Sugathan / Pandian, Shunmugiah Karutha

    Archives of microbiology. 2022 May, v. 204, no. 5

    2022  

    Abstract: Biofilm formation is a major issue in healthcare settings as 75% of nosocomial infection arises due to biofilm residing bacteria. Exopolysaccharides (EPS), a key component of the biofilm matrix, contribute to the persistence of cells in a complex milieu ... ...

    Abstract Biofilm formation is a major issue in healthcare settings as 75% of nosocomial infection arises due to biofilm residing bacteria. Exopolysaccharides (EPS), a key component of the biofilm matrix, contribute to the persistence of cells in a complex milieu and defends greatly from exogenous stress and demolition. It has been shown to be vital for biofilm scaffold and pathogenic features. The present study was aimed to investigate the effectiveness of four domain-containing α-amylase from Streptomyces griseus (SGAmy) in disrupting the EPS of multidrug-resistant bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. In vitro analysis of preformed biofilm unveiled the antibiofilm efficacy of SGAmy against MRSA (85%, p < 0.05) and P. aeruginosa (82%, p < 0.05). The total carbohydrate content in the EPS matrix of MRSA and P. aeruginosa was significantly reduced to 71.75% (p < 0.01) and 74.09% (p < 0.01), respectively. The findings inferred from in vitro analysis were further corroborated through in vivo studies using an experimental model organism, Danio rerio. Remarkably, the survival rate was extended to 88.8% (p < 0.05) and 74.2% (p < 0.05) in MRSA and P. aeruginosa infected fishes, respectively. An examination of gills, kidneys, and intestines of D. rerio organs depicted the reduced level of microbial colonization in SGAmy-treated cohorts and these findings were congruent with bacterial enumeration results.
    Schlagwörter Danio rerio ; Pseudomonas aeruginosa ; Streptomyces griseus ; alpha-amylase ; biofilm ; carbohydrate content ; cross infection ; exopolysaccharides ; health services ; methicillin-resistant Staphylococcus aureus ; microbial colonization ; models ; multiple drug resistance ; plate count ; survival rate
    Sprache Englisch
    Erscheinungsverlauf 2022-05
    Umfang p. 243.
    Erscheinungsort Springer Berlin Heidelberg
    Dokumenttyp Artikel
    ZDB-ID 124824-8
    ISSN 1432-072X ; 0302-8933
    ISSN (online) 1432-072X
    ISSN 0302-8933
    DOI 10.1007/s00203-022-02847-4
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  9. Artikel ; Online: Evaluation of antibiofilm potential of four-domain α-amylase from Streptomyces griseus against exopolysaccharides (EPS) of bacterial pathogens using Danio rerio.

    Lakshmi, Selvaraj Alagu / Alexpandi, Rajaiah / Shafreen, Raja Mohamed Beema / Tamilmuhilan, Kannapiran / Srivathsan, Adimoolam / Kasthuri, Thirupathi / Ravi, Arumugam Veera / Shiburaj, Sugathan / Pandian, Shunmugiah Karutha

    Archives of microbiology

    2022  Band 204, Heft 5, Seite(n) 243

    Abstract: Biofilm formation is a major issue in healthcare settings as 75% of nosocomial infection arises due to biofilm residing bacteria. Exopolysaccharides (EPS), a key component of the biofilm matrix, contribute to the persistence of cells in a complex milieu ... ...

    Abstract Biofilm formation is a major issue in healthcare settings as 75% of nosocomial infection arises due to biofilm residing bacteria. Exopolysaccharides (EPS), a key component of the biofilm matrix, contribute to the persistence of cells in a complex milieu and defends greatly from exogenous stress and demolition. It has been shown to be vital for biofilm scaffold and pathogenic features. The present study was aimed to investigate the effectiveness of four domain-containing α-amylase from Streptomyces griseus (SGAmy) in disrupting the EPS of multidrug-resistant bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. In vitro analysis of preformed biofilm unveiled the antibiofilm efficacy of SGAmy against MRSA (85%, p < 0.05) and P. aeruginosa (82%, p < 0.05). The total carbohydrate content in the EPS matrix of MRSA and P. aeruginosa was significantly reduced to 71.75% (p < 0.01) and 74.09% (p < 0.01), respectively. The findings inferred from in vitro analysis were further corroborated through in vivo studies using an experimental model organism, Danio rerio. Remarkably, the survival rate was extended to 88.8% (p < 0.05) and 74.2% (p < 0.05) in MRSA and P. aeruginosa infected fishes, respectively. An examination of gills, kidneys, and intestines of D. rerio organs depicted the reduced level of microbial colonization in SGAmy-treated cohorts and these findings were congruent with bacterial enumeration results.
    Mesh-Begriff(e) Animals ; Anti-Bacterial Agents/pharmacology ; Bacteria ; Biofilms ; Methicillin-Resistant Staphylococcus aureus ; Microbial Sensitivity Tests ; Pseudomonas aeruginosa ; Streptomyces griseus ; Zebrafish ; alpha-Amylases
    Chemische Substanzen Anti-Bacterial Agents ; alpha-Amylases (EC 3.2.1.1)
    Sprache Englisch
    Erscheinungsdatum 2022-04-05
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 124824-8
    ISSN 1432-072X ; 0302-8933
    ISSN (online) 1432-072X
    ISSN 0302-8933
    DOI 10.1007/s00203-022-02847-4
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: CRISPR based development of RNA editing and the diagnostic platform.

    Satish, Lakkakula / Lavanya, Gunamalai / Kasthuri, Thirupathi / Kalaivaani, Aruchamy / Shamili, Sasanala / Muthuramalingam, Pandiyan / Gowrishankar, Shanmugaraj / Pandian, Shunmugiah Karutha / Singh, Vijai / Sitrit, Yaron / Kushmaro, Ariel

    Progress in molecular biology and translational science

    2021  Band 179, Seite(n) 117–159

    Abstract: Clustered Regularly Interspersed Short Palindromic Repeat-CRISPR-Associated (CRISPR-Cas) system has improved the ability to edit and control gene expression as desired. Genome editing approaches are currently leading the biomedical research with improved ...

    Abstract Clustered Regularly Interspersed Short Palindromic Repeat-CRISPR-Associated (CRISPR-Cas) system has improved the ability to edit and control gene expression as desired. Genome editing approaches are currently leading the biomedical research with improved focus on direct nuclease dependent editing. So far, the research was predominantly intended on genome editing over the DNA level, recent adapted techniques are initiating to secure momentum through their proficiency to provoke modifications in RNA sequence. Integration of this system besides to lateral flow method allows reliable, quick, sensitive, precise and inexpensive diagnostic. These interesting methods illustrate only a small proportion of what is technically possible for this novel technology, but several technological obstacles need to be overcome prior to the CRISPR-Cas genome editing system can meet its full ability. This chapter covers the particulars on recent advances in CRISPR-Cas9 genome editing technology including diagnosis and technical advancements, followed by molecular mechanism of CRISPR-based RNA editing and diagnostic tools and types, and CRISPR-Cas-based biosensors.
    Mesh-Begriff(e) Base Sequence ; CRISPR-Cas Systems/genetics ; DNA ; Humans ; RNA Editing/genetics
    Chemische Substanzen DNA (9007-49-2)
    Sprache Englisch
    Erscheinungsdatum 2021-02-15
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 2471995-X
    ISSN 1878-0814 ; 0079-6603 ; 1877-1173
    ISSN (online) 1878-0814
    ISSN 0079-6603 ; 1877-1173
    DOI 10.1016/bs.pmbts.2020.12.015
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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