Article ; Online: Milk Allergen Micro-Array (MAMA) for Refined Detection of Cow's-Milk-Specific IgE Sensitization.
2023 Volume 15, Issue 10
Abstract: Background: Immunoglobulin-E(IgE)-mediated hypersensitivity to cow's milk allergens is a frequent cause of severe and life-threatening anaphylactic reactions. Besides case histories and controlled food challenges, the detection of the IgE antibodies ... ...
| Abstract | Background: Immunoglobulin-E(IgE)-mediated hypersensitivity to cow's milk allergens is a frequent cause of severe and life-threatening anaphylactic reactions. Besides case histories and controlled food challenges, the detection of the IgE antibodies specific to cow's milk allergens is important for the diagnosis of cow-milk-specific IgE sensitization. Cow´s milk allergen molecules provide useful information for the refined detection of cow-milk-specific IgE sensitization. Methods: A micro-array based on ImmunoCAP ISAC technology was developed and designated milk allergen micro-array (MAMA), containing a complete panel of purified natural and recombinant cow's milk allergens (caseins, α-lactalbumin, β-lactoglobulin, bovine serum albumin-BSA and lactoferrin), recombinant BSA fragments, and α-casein-, α-lactalbumin- and β-lactoglobulin-derived synthetic peptides. Sera from 80 children with confirmed symptoms related to cow's milk intake (without anaphylaxis: Results: The use of MAMA allowed a component-resolved diagnosis of IgE sensitization in each of the children suffering from cow's-milk-related anaphylaxis according to Sampson grades 1-5 requiring only 20-30 microliters of serum. IgE sensitization to caseins and casein-derived peptides was found in each of the children with Sampson grades of 4-5. Among the grade 1-3 patients, nine patients showed negative reactivity to caseins but showed IgE reactivity to alpha-lactalbumin ( Conclusions: The use of MAMA allows for the detection, using only a few microliters of serum, of IgE sensitization to multiple cow's milk allergens and allergen-derived peptides in cow-milk-allergic children with cow-milk-related anaphylaxis. |
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| MeSH term(s) | Animals ; Female ; Cattle ; Milk ; Allergens ; Caseins ; Lactalbumin ; Anaphylaxis/diagnosis ; Milk Hypersensitivity ; Immunoglobulin E ; Peptides ; Lactoglobulins ; Milk Proteins |
| Chemical Substances | Allergens ; Caseins ; Lactalbumin (9013-90-5) ; Immunoglobulin E (37341-29-0) ; Peptides ; Lactoglobulins ; Milk Proteins |
| Language | English |
| Publishing date | 2023-05-21 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2518386-2 |
| ISSN | 2072-6643 ; 2072-6643 |
| ISSN (online) | 2072-6643 |
| ISSN | 2072-6643 |
| DOI | 10.3390/nu15102401 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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